检索结果-内蒙古大学图书馆

您好，读者！请登录

内蒙古大学图书馆

首页
概况
党建
资源
服务
科研支持
- 论文收录引用证明
- 科技查新
知识产权
档案馆
帮助

咨询与建议

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

您的常用邮箱：*

您的手机号码：*

问题描述：

当前已输入0个字，您还可以输入200个字

全部搜索
期刊论文
图书
学位论文
标准
纸本馆藏
外文资源发现
数据库导航
超星发现

高级检索

时间限定

出版年份：

文献类型

图书期刊文献学位论文多媒体

馆藏选择

电子馆藏纸本馆藏

核心期刊

全部期刊 SCI 收录期刊 SSCI 收录期刊 EI 收录期刊 CSCD 收录期刊 CSSCI 收录期刊

语言

中文英文

文献类型

期刊文献图书学位论文标准纸本馆藏

帮助

文字说明：

T=题名（书名、题名），A=作者（责任者），K=主题词，P=出版物名称，PU=出版社名称，O=机构（作者单位、学位授予单位、专利申请人），L=中图分类号，C=学科分类号，U=全部字段，Y=年（出版发行年、学位年度、标准发布年）

检索规则说明：

AND代表“并且”；OR代表“或者”；NOT代表“不包含”；(注意必须大写,运算符两边需空一格)

检索范例：

范例一：(K=图书馆学 OR K=情报学) AND A=范并思 AND Y=1982-2016
范例二：P=计算机应用与软件 AND (U=C++ OR U=Basic) NOT K=Visual AND Y=2011-2016

分类表

所选分类

>> <<

限定检索结果

文献类型

826 篇 会议
18 篇 期刊文献

馆藏范围

844 篇 电子文献
0 种 纸本馆藏

日期分布

学科分类号

566 篇 医学
- 330 篇 公共卫生与预防医...
- 237 篇 临床医学
- 26 篇 基础医学(可授医学...
- 25 篇 医学技术（可授医学...
- 25 篇 护理学（可授医学、...
- 21 篇 药学(可授医学、理...
- 9 篇 中西医结合
- 5 篇 中医学
- 2 篇 中药学(可授医学、...
7 篇 工学
- 4 篇 生物医学工程（可授...
- 3 篇 材料科学与工程（可...
2 篇 管理学
- 2 篇 公共管理

主题

86 篇 hbv
57 篇 慢性乙型肝炎
37 篇 肝衰竭
35 篇 抗病毒治疗
32 篇 hbeag
21 篇 hcv
17 篇 肝性脑病
16 篇 慢加急性肝衰竭
16 篇 乙型肝炎
15 篇 乙型肝炎病毒
14 篇 慢性乙型肝炎患者
14 篇 hbsag
14 篇 肝硬化
14 篇 chb
14 篇 拉米夫定
14 篇 重型肝炎
12 篇 慢性丙型肝炎
12 篇 感染者
12 篇 乙肝病毒
11 篇 临床意义

机构

112 篇 第三军医大学西南...
23 篇 解放军第302医院
22 篇 第三军医大学附属...
21 篇 重庆医科大学附属...
20 篇 重庆医科大学附属...
17 篇 第四军医大学唐都...
17 篇 华中科技大学同济...
15 篇 上海交通大学医学...
15 篇 浙江大学医学院附...
13 篇 首都医科大学附属...
12 篇 复旦大学
10 篇 北京大学第一医院
10 篇 青岛市传染病医院
10 篇 第三军医大学第一...
10 篇 南方医科大学南方...
10 篇 深圳市第三人民医...
10 篇 解放军302医院
10 篇 institute of inf...
10 篇 解放军第三○二医院...
9 篇 中国医科大学附属...

作者

73 篇 王宇明
28 篇 毛青
26 篇 王小红
26 篇 邓国宏
21 篇 辛绍杰
20 篇 郑瑞丹
18 篇 yuming wang
18 篇 谭朝霞
18 篇 朱研
17 篇 王慧芬
16 篇 贾战生
16 篇 侯金林
15 篇 张长江
15 篇 何登明
15 篇 游绍莉
15 篇 宁琴
15 篇 qin ning
13 篇 臧红
13 篇 任红
12 篇 徐成润

语言

721 篇 中文
123 篇 英文

检索条件"任意字段=中华医学会病毒性肝炎慢性化、重症化基础与临床研究进展学术会议"

共 844 条记录，以下是491-500 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

Long-term Clinical Outcomes after Withdrawal of Nucleos(t)ide Analogues in Patients with Chronic Hepatitis B

Long-term Clinical Outcomes after Withdrawal of Nucleos(t)id...

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：何登明 Xianli Chen Guohua Yan Jie Wang Maoshi Li Shimin Guo Peng Zhu Hongfei Huang Yuming Wang Institute of Infectious Diseases Southwest HospitalThird Military Medical University

The management strategy of chronic hepatitis B in patients treated with nucleos(t)ide analogues(NAs) is a topic of *** achieving specific endpoints,the outcome after withdrawal of NAs was *** 2002 and 2009,a total of 155 patients who achieved hepatitis B surface antigen(HBsAg) loss,hepatitis B e antigen(HBeAg) seroconversion(for HBeAg-positive patients),or maintained undetectable HBV DNA(for HBeAg-negative patients) and had discontinued treatment for at least 96 weeks were included in the *** DNA and alanine aminotransferase(ALT) level were monitored regularly after withdrawal of treatment. Relapse was determined by twice continuous HBV DNA over 200 IU/mL and ALT over 2 time upper limits of *** median duration of consolidation treatment was 120 weeks (interquartile range,72-144 weeks),96 weeks(interquartile range,48-144 weeks) in HBeAg-positive patients and 144 weeks(interquartile range,96-168 weeks) in HBeAg-negative *** the 155 patients,24(16%) demonstrated evidence of relapse(8%of HBeAg-positive and 28%of HBeAg-negative,P<0.01).No relapse was observed in HBsAg loss *** of relapses occurred within 96 weeks of *** median duration of relapse was 36 weeks(interquartile range,13-48 weeks).Elevation of HBV DNA and ALT level over baseline was only observed in approximately 10%of relapse *** was no significant difference of the baseline characteristic(sex,HBV genotype,age,medicine,or ALT level) and duration of treatment between relapse patients and sustained response *** withdrawal is feasible after achieving some specific *** time of consolidation treatment in HBeAg-positive patients and maintaining consistent undetectable HBV DNA in HBeAg-negative patients are effective strategy to reduce the relapse rate after *** than 96 weeks of sustained response after withdrawal is a predictive marker for long-term sustained response.

关键词： HBeAg HBV t)ide Analogues in Patients with Chronic Hepatitis B Long-term Clinical Outcomes after Withdrawal of Nucleos

来源：评论

学校读者我要写书评

暂无评论

Role of A20 in IFN-α-mediated functional restoration of myeloid dendritic cells in hepatitis C virus infection

Role of A20 in IFN-α-mediated functional restoration of mye...

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：张颖 Li Ma Yun Zhou Yonghong Guo Yu He Jiuping Wang Jianqi Lian Chunqiu Hao Zhansheng Jia Department of Infectious Diseases Tangdu HospitalThe Fourth Military Medical University

Hepatitis C virus(HCV) infection places a heavy burden on health care services worldwide. More than 80%of patients infected with HCV show chronic infection and immunosuppression, which may be due to defects in antigen presentation by myeloid dendritic cells(mDCs).These defects appear to be remedied by treatment with interferon alpha(IFN-α)-based antiviral therapies. However,the molecular mechanisms underlying mDC dysfunction in HCV infection and restoration by IFN-αtreatment are *** ubiquitin-editing protein,A20,which negatively modulates NF-κB signaling,plays a crucial role in controlling the maturation,cytokine production,and immunostimulatory potency of *** thus propose that A20 expression is correlated with the function of mDCs during HCV chronic infection by IFN-α*** present study demonstrated that A20 expression in mDCs isolated from chronically HCV-infected patients was significantly higher than that in healthy subjects or patients achieving SVR(sustained virological response) following ***,A20 expression in cultured mDCs in response to poly-Ⅰ:C treatment differed between HCV patients and healthy subjects;this difference was abrogated by treatment with exogenous IFN-α.A20 expression by poly-Ⅰ:C-activated mDCs(either with or without IFN-αtreatment) was negatively correlated with the expression of HLA-DR,CD86 and CCR7,and with the production of IL-12,but positively correlated with the production of IL-10. Silencing A20 expression using small interfering RNAs increased the production of IL-12 production in mDCs of chronically HCV-infected *** findings suggest that A20 plays a crucial role in negative regulation of innate immune responses during chronic viral infection and may be a novel target for immunotherapy to HCV infection.

关键词： Hepatitis C virus chronic infection myeloid dendritic cells IFN-α A20 anti-HCV therapy

来源：评论

学校读者我要写书评

暂无评论

肝硬化失代偿期患者细菌感染及治疗现状

肝硬化失代偿期患者细菌感染及治疗现状

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：丁胜楠杜娜杨伟民牛俊奇吉林大学第一医院肝病科

肝硬化患者因免疫功能低下、肠道通透性增加等因素易发生各种机会性感染。失代偿期肝硬化患者较代偿期肝硬化患者更易发生细菌感染。国内最常见的感染是自发性细菌性腹膜炎,其次是呼吸道感染、肠道感染、菌血症或者败血症、尿路感染以... 详细信息

肝硬化患者因免疫功能低下、肠道通透性增加等因素易发生各种机会性感染。失代偿期肝硬化患者较代偿期肝硬化患者更易发生细菌感染。国内最常见的感染是自发性细菌性腹膜炎,其次是呼吸道感染、肠道感染、菌血症或者败血症、尿路感染以及皮肤软组织感染等。患者合并细菌感染后病死率明显升高,及时合理的抗感染治疗能够提高治愈率,缩短住院时间,减少治疗费用。目前推荐第三代头孢菌素为一线用药。然而,首选的抗生素对院内感染的有效性要明显低于社区获得性感染。这可能与多重耐药细菌尤其是产超广谱β内酰胺酶肠杆菌在院内感染中逐渐增多有关。在细菌培养及药敏结果回报前,第三代头孢菌素仍是许多社区获得性感染的首选药物,而院内感染应根据当地抗生素的耐药情况选择经验性治疗的抗生素。抗生素预防治疗容易发生细菌耐药,但消化道出血后,既往有SBP病史,或既往无SBP病史但腹水蛋白<15g/L患者,仍推荐预防性给予抗生素治疗。应用抗生素治疗自发性细菌性腹膜炎,如同时静脉输注白蛋白可减少肝肾综合征的发生,减少病死率。

关键词：肝硬化病原菌耐药

来源：评论

学校读者我要写书评

暂无评论

Interference with novel gene KCTD9inhibits NK cell activation and ameliorates Murine Hepatitis Virus Type 3-induced fulminant hepatitis in BALB/cJmice

Interference with novel gene KCTD9inhibits NK cell activatio...

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：陈韬 Lin Zhu Lin Ding Li Song Xiaoping Zhang Aichao Shi Hui Guo Li Zhou Yaoyong Zhou Bin Pi Xiaojuan Liu Xiaoping Luo Qin Ning Department and Institute of Infectious Disease Department of Pediatric DiseaseTongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

Background/aims:Our previous reports,both experimental and human studies,have shown the novel geneKCTD9 contributed to liver injuiythrough hepatic NK cell activation in HBV induced acute-on-chronic liver *** study aims to elucidate the therapeutic role of KCTD9 in a mice model. Methods:murine hepatitis virus strain 3(MHV-3) induced fulminant viral hepatitis (MHV-3-FVH) mice model was adopted in the *** mouse KCTD9(mKCTD9) expression plasmid and anshRNA plasmid specifically targeting this molecule were constructed and introduced into infected mice by hydrodynamic *** expression of KCTD9 as well as function of hepatic NK cells was detected,respectively. Results:The results showed abundant expression of KCTD9 in hepatic NK cells from mice with MHV-3 *** significantly inhibited KCTD9 expression in hepatic NK cells with decreased CD69 expression,cytotoxicity andameliorated MHV-3-FVHin these micedemonstratingas an increased survival,improved liver functions and histopathology *** contrast,delivery of the KCTD9 expression plasmid to MHV-3-infected mice led to a profound progression of liver disease. Conclusion:Our study further elaborated the novel KCTD9 gene contributes to liver injury through NK cell activation in virus-induced liver failure,while interference targeting KCTD9 gene could ameliorated the disease,which provide a potential therapeutic target for virus induced liver failure.

关键词： Interference with novel gene KCTD9inhibits NK cell activation and ameliorates Murine Hepatitis Virus Type 3-induced fulminant hepatitis in BALB/cJmice

来源：评论

学校读者我要写书评

暂无评论

A Common Promoter Variant of TBX21 is Associated with Allele Specific Binding to Yin-Yang 1 and Reduced Gene Expression

A Common Promoter Variant of TBX21 is Associated with Allele...

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：李继荣 Medical Team Force 75135 of Chinese People's Liberation ArmyGuigang Municipality

T-bet is a key regulator for the lineage commitment in CD4 T helper(Th) 1 cells by activating the hallmark production of interferon-c,and its expression level is linked to autoimmune,infectious, and allergic diseases.A T to C base substitution has been identified at position -1993 in the TBX21(encoding T-bet) promoter and has been associated with asthma and systemic lupus *** study aimed to investigate the molecular mechanisms responsible for the influence of the T-1993C polymorphism on transcription and its functional effect by luciferase reporter,EMSAs,Chromatin immunoprecipitation assay,and flow cytometric analysis of intracellular T-bet,IFN-c and IL-4 expression in activated CD4+ T *** presence of a -1993T allele obviously increases promoter activity compared with that of a promoter with a - 1993C allele. TBX21 promoter carrying -1993C allele possesses significantly stronger binding affinity to the Yin Yang 1(YY1) transcription factor than that carrying -1993T ***1 overexpression decreased TBX21 promoter function in a T cell line, demonstrating that this element functions as a *** C to T base exchange relieves the repression mediated by *** individuals carrying -1993C allele were determined to have significantly diminished expression of TBX21 and IFN-c and increased IL-4 production in cells compared with the individuals carrying -1993T allele(P < 0.05).These findings demonstrate that the TBX21 T-1993C polymorphism represses TBX21 expression and Th1 cytokine production through control of YY1,which might result in the imbalance between Th1 and Th2 immune responses in autoimmune or allergic diseases.

关键词： A Common Promoter Variant of TBX21 is Associated with Allele Specific Binding to Yin-Yang 1 and Reduced Gene Expression

来源：评论

学校读者我要写书评

暂无评论

Relationship between HLA-DP Gene Polymorphisms and Clearance of Chronic Hepatitis B Virus Infections

Relationship between HLA-DP Gene Polymorphisms and Clearance...

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：晏泽辉 Shun Tan Guohong Deng Yuming Wang Institute of Infectious Diseases Southwest HospitalThird Military Medical University

Hepatitis B virus(HBV) infection is a serious public health problem *** common genetic variants(rs3077 and rs9277535) of Human Leukocyte Antigen DP(HLA-DP) have been reported to be associated with persistent HBV infection in populations of Japan and Thailand. To confirm whether the association can be replicated in Chinese populations,an independent case-control study were conducted,and two polymorphisms(rs3077 and rs9277535) were genotyped using the TaqMan SNP genotyping assay in 282 persistent chronic HBV carriers and 64 spontaneously HBV recovered *** provide a more definitive conclusion,a meta-analysis combining and summarizing 5 studies was performed by random-effects model using the DerSimonian and Laird's *** using logistic regression analysis with adjustment for covariates,including age,sex,and alcohol consumption,the results of our independent case-control study showed that the minor allele's homozygote(AA genotype) of rs3077 and rs9277535 was significantly associated with decreasing risk/protection of HBV persistent chronic infection(for rs3077:P= 0.0017,OR=0.29,95%CI =0.13-0.62;for rs9277535,P= 0.0004,OR =0.26,95%CI =0.12-0.54).The results of meta-analysis pooling all eligible studies also showed that rs3077-A and rs9277535-A alleles were associated with an increased clearance rate to HBV infection(rs3077:OR =0.57,95%CI=0.44-0.75;rs9277535:OR =0.56,95%CI= 0.47-0.63).These results further confirmed the strong influence of HLA-DP gene variants on risk of spontaneous HBV clearance from persistent HBV *** A alleles of HLA-DP SNP rs3077 and rs9277535 showed strong protective effects for spontaneous HBV clearance from persistent HBV infection in the Han Chinese population.

关键词： Hepatitis B virus(HBV) Human Leukocyte Antigens DP(HLA-DP) Meta-analysis Polymorphism

来源：评论

学校读者我要写书评

暂无评论

Quantitative hepatitis B surface antigen titers in Chinese chronic hepatitis B patients over 4 years of entecavir treatment

Quantitative hepatitis B surface antigen titers in Chinese c...

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：陈恩强唐红王婷婷白浪陶传敏梁韬刘聪廖娟四川大学华西医院感染性疾病中心

Background & Aims:The clinical value of quantitative hepatitis B surface antigen(qHBsAg) titer in patients taking nucleot(s)ide analogs(NAs) is still *** study aims to investigate the dynamic changes of qHBsAg titers and its significance for predicting virological response(VR) and serological response(SR) to long-term entecavir(ETV) treatment. Methods:Forty-eight ETV-nai've patients were enrolled and followed prospectively for 4 years, and 32 of them were HBeAg-positive at *** alanine aminotransferase(ALT),qualitative hepatitis B virus(HBV) serological markers,and HBV DNA were detected;and qHBsAg titers were measured using Elecsys? HBsAgⅡQuant Assay. Results:The mean baseline HBV-DNA and qHBsAg were 7.51 log copies/mL and 3.78 log IU/mL,*** 48 months of ETV treatment,the rates of VR(<291 copies/mL),ALT normalization and SR(HBeAg/anti-HBe) were 89.6%(43/48),89.6%(43/48) and 34.4%(11/32), *** was a decline in qHBsAg titers from baseline to months 48,ranging from 3.78 to 3.10 log IU/*** greatest decline of HBsAg was observed in the first 3 months of treatment (0.47 log10 IU/mL),which was significantly correlated with corresponding HBV-DNA decreasing (3.89 log copies/mL)(P=0.032).By using ROC analysis,qHBsAg titers at baseline(AUC=0.647) and 3 months after treatment(AUC=0.586) had poor power in predicting of 48-month VR;qHBsAg titers at baseline(AUC=0.779) and 3 months after ETV treatment(AUC=0.658) had poor power in predicting of 48-month SR in HBeAg-positive patients at ***,the declines of qHBsAg in the first 3 months of treatment also had poor power in predicting of either 48-month VR or SR. Conclusions:ETV is efficacious in NAs-nai've patients,and qHBsAg titers declined significantly in the first 3 months of ETV ***,qHBsAg titer is not a good predictor of 4-year virological and HBeAg/anti-HBe serological responses in this cohort.

关键词： Quantitative hepatitis B surface antigen titers in Chinese chronic hepatitis B patients over 4 years of entecavir treatment

来源：评论

学校读者我要写书评

暂无评论

混合型生物人工肝治疗HBV相关慢加急性肝衰竭患者的初步探讨

混合型生物人工肝治疗HBV相关慢加急性肝衰竭患者的初步探讨

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：游绍莉刘鸿凌荣义辉朱冰臧红刘婉姝万志红貌盼勇辛绍杰解放军第三○二医院

目的:利用自行构建的混合型生物人工肝系统,探讨其治疗HBV相关慢加急性肝衰竭患者的安全性和有效性。方法:采用转染人肝再生增强因子(hALR)的HepG2细胞为生物材料,构建中空纤维生物反应器。2009年5月-2011年8月于我院住院HBV相关慢加急... 详细信息

目的:利用自行构建的混合型生物人工肝系统,探讨其治疗HBV相关慢加急性肝衰竭患者的安全性和有效性。方法:采用转染人肝再生增强因子(hALR)的HepG2细胞为生物材料,构建中空纤维生物反应器。2009年5月-2011年8月于我院住院HBV相关慢加急性肝衰竭患者作为治疗对象,随机分为2组,每组10例,治疗组进行混合型生物人工肝治疗,对照组进行普通血浆置换治疗。应用SAS统计软件进行分析,两组间均数比较采用成组t检验,治疗前后比较采用配对t检验。结果:治疗组10例患者中,7例经住院治疗临床好转出院,其余1例因肝性脑病死亡,1例因肝肾综合征死亡,1例出院后死于肝衰竭。对照组10例患者中存活5例,其余1例肝移植,4例因肝衰竭死亡。2组患者治疗前MELD评分分别为24.26+2.54及24.71+2.79,差异无统计学意义(t=1.971,P=0.064)。治疗组治疗3d、1、4周MELD评分平均分别为21.71±2.92、22.10±4.46、19.90±5.43。跟踪随访1a,治疗组及对照组患者血清甲胎蛋白平均值为14.24 ng/ml及11.32 ng/ml,腹部B超检查均未发现肝脏占位性病变。结论:自行构建的混合型生物人工肝支持系统治疗HBV相关慢加急性肝衰竭患者具有一定安全性和有效性。

关键词：肝衰竭生物人工肝治疗

来源：评论

学校读者我要写书评

暂无评论

自然免疫状态下HCV病毒E2区宿主内及宿主间病毒进化规律

自然免疫状态下HCV病毒E2区宿主内及宿主间病毒进化规律

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：熊瑜琳朱研张长江袁婧胡亚君谭朝霞王小红第三军医大学西南医院全军感染病研究所

目的:分析自然免疫压力下慢性1b型HCV感染者E2基因宿主内及宿主间病毒进化模式及规律,探索HCV免疫逃逸的序列变异规律及特征性变异位点。方法:未抗病毒治疗的14例慢性1b型HCV感染者及其系列血清样本均来自中国人HCV感染患者系列标本库(C... 详细信息

目的:分析自然免疫压力下慢性1b型HCV感染者E2基因宿主内及宿主间病毒进化模式及规律,探索HCV免疫逃逸的序列变异规律及特征性变异位点。方法:未抗病毒治疗的14例慢性1b型HCV感染者及其系列血清样本均来自中国人HCV感染患者系列标本库(CQueen cohort)。对每例感染者3个连续时间点(间隔半年以上)的血清样本进行HCV 5'端6 000bp基因组的扩增、克隆,每个时间点随机挑选25～33个克隆对E2基因测序,用MEGA软件及MJN进行序列及系统进化分析,Accelrys GCG进行序列的装备及遗传距离的分析,HyPhy进行选择压力的分析。结果:10HCV慢性感染者3个不同时间点血清标本成功获得HCV C-NS3A区6 030 bp的目的片段,4例患者获得2个不同时间点HCV C-NS3片段。所有时间点的C-NS3A区6 030bp均成功克隆,并完成所有准种克隆(1117株)E2区(396bp)序列测定,确立了动态优势变异株。慢性HCV感染者体内病毒准种的复杂程度并非随着感染时间的延长而进行性增加,在某个时间点上准种变异株的序列可能会趋于一致,不同时间点存在不同优势变异株,一些变异株可长期存在宿主体内。不同时间点HCV核苷酸差异度进展不同,大多呈现起伏状态。感染过程中,多数患者始终处于负选择压力状态下,部分感染者经历正选择压力后,进入到负选择压力状态下。结论:自然免疫状态下,不同时间宿主体内HCV存在不同的优势变异株。不同感染者,不同感染时期,HCV进化模式及进化频率存在差异;HCV慢性感染过程中,病毒的变异和进化多是在负选择压力下进行,病毒载量的持续可能和有效地中和免疫应答降低相关,而并非HCV适应性的增强。研究揭示了HCV感染者体内病毒复杂的动态变化及进化模式,为HCV C-NS3基因序列的分析奠定了良好的基础。

关键词：丙型肝炎病毒包膜2基因适应性进化优势变异株

来源：评论

学校读者我要写书评

暂无评论

干扰素对慢性乙型肝炎患者外周血单个核细胞hTERT mRNA表达的影响

干扰素对慢性乙型肝炎患者外周血单个核细胞hTERT mRNA表达的影响

引用

第三届全国病毒性肝炎慢性化重症化基础与临床研究进展学术会议

作者：朱传武罗湘蓉吴建鸿王海燕朱莉李明杨齐英朱伟张雪华叶建中常建国胥萍陈明王丽华苏州市第五人民医院肝病科徐州医学院附属医院感染病科

目的:探讨干扰素治疗对慢性乙型肝炎(CHB)患者外周血单个核细胞(PBMCs)表达hTERT mRNA的影响。方法:用淋巴细胞分离液分离37例干扰素治疗CHB患者(干扰素治疗组),36例CHB对照患者(患者对照组)和10例健康人(健康对照组)的PBMCs,用Real-tim... 详细信息

目的:探讨干扰素治疗对慢性乙型肝炎(CHB)患者外周血单个核细胞(PBMCs)表达hTERT mRNA的影响。方法:用淋巴细胞分离液分离37例干扰素治疗CHB患者(干扰素治疗组),36例CHB对照患者(患者对照组)和10例健康人(健康对照组)的PBMCs,用Real-time PCR方法定量检测各组PBMCs的hTERTmRNA及其相应的β-actinmRNA,计算出标化的hTERT mRNA(NhTERT mRNA);用Taqman荧光定量PCR方法检测患者HBV DNA载量;用Real-timePCR-荧光探针技术检测HBV基因型;用流式细胞技术检测T淋巴细胞亚群。结果 PBMCs表达NhTERT mRNA的水平与研究对象年龄、患者ALT和HBV DNA未见显著相关性关系(均为P>0.05),而与患者CD3+T淋巴细胞和CD4+/CD8+比值呈显著正相关关系(分别为P=0.026和P=0.041);不同HBV基因型组别之间NhTERT mRNA的均值无显著性差异(P=0.664);正常对照组、患者对照组和干扰素治疗组NhTERT mRNA的均值分别为1.042×10-3,0.857×10-3和0.959×10-3,健康对照组显著高于患者对照组(P<0.001)和干扰素治疗组(P=0.041),而干扰素治疗组的均值显著高于患者对照组(P=0.001)。干扰素治疗后获得HBeAg转阴患者的NhTERT mRNA均值显著高于未转阴患者(P=0.015),且与健康对照组比较未见显著性差异(P=0.479),而在HBV DNA载量小于3 logl0组和ALT正常组中,NhTERT mRNA的均值与其相应组比较均未达到统计学差异(分别为P=0.059和P=0.249)。结论本研究发现CHB患者PBMCs表达hTERT mRNA的水平与T淋巴细胞总数和CD4+/CD8+比值具有密切的关系;患者PBMCs表达的端粒酶活性低于健康人,干扰素抗病毒治疗可以上调hTERT mRNA的表达,尤其是在获得HBeAg转阴的患者中hTERT mRNA的表达可以达到健康人水平。提示在CHB的抗病毒治疗中干扰素还能够以上调淋巴细胞端粒酶活性的方式增强患者的免疫功能。

关键词：干扰素治疗 CHB 慢性乙型肝炎患者 hTERT mRNA 外周血单个核细胞

来源：评论

学校读者我要写书评

暂无评论

没有更多数据了...

全选清除本页清除全部题录导出标记到“检索档案”

共85页 << < 46 47 48 49 50 51 52 53 54 55 > >>

检索报告对象比较合并检索0

隐藏清空

合并搜索

回到顶部

执行限定条件

内容：

评分：

请选择保存的检索档案：

请选择收藏分类：

订阅名称：

通借通还

温馨提示：

图书名称：

借书校区：

取书校区：

手机号码：

邮箱地址：

一卡通帐号：

电话和邮箱必须正确填写，我们会与您联系确认。

联系人：

所在院系：

联系邮箱：

联系电话：

内蒙古自治区呼和浩特市赛罕区大学西街235号邮编: 010021

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：