Insulin like growth factors-Ⅰ(IGF- 1) is essential for longitudinal bone growth,as it stimulates proliferation and differentiation of chondrocytes in the epiphyseal *** aim of this study is to measure the serum IGF-I...
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Insulin like growth factors-Ⅰ(IGF- 1) is essential for longitudinal bone growth,as it stimulates proliferation and differentiation of chondrocytes in the epiphyseal *** aim of this study is to measure the serum IGF-I level and BMD in postmenopausal osteoporotic *** IGF-Ⅰlevel of 50 postmenopausal osteoporotic women(≥50 years) with
目的:近年来研究发现骨质疏松患者骨髓中有大量脂肪细胞浸润且与骨量减少与骨丢失是成正比的。本实验旨在研究骨髓间充质干细胞来源的脂肪细胞在成骨细胞发生分化及骨形成过程中的抑制作用机制。方法:Ⅰ.细胞共培养使用三种不用浓度的脂肪细胞诱导剂诱导骨髓间充质干细胞向脂肪细胞方向分化,14天后与骨髓间充质干细胞共培养,同时向成骨细胞分化方向诱导。共培养有两种模式:脂肪细胞和干细胞直接接触(ModeA)和非直接接触(Mode B)。Ⅱ.形态学分析细胞向成骨细胞分化方向诱导14天后进行碱性磷酸酶(ALP)染色,28天后进行钙化结节染色。Ⅲ.mRNA microarray分别收集Mode B 3组下层细胞提取RNA进行比较分析。ⅣTwo-dimensional Gel Electro-
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