@@ Enzyme-linked immunosorbent assay(ELISA) usually tolerates a nalTOW range to quantify an analyte of *** routine practice。activities of enzymes as labels in ELISA ale exclusively measured by the classical initial r...
@@ Enzyme-linked immunosorbent assay(ELISA) usually tolerates a nalTOW range to quantify an analyte of *** routine practice。activities of enzymes as labels in ELISA ale exclusively measured by the classical initial rate method using synthetic chromogenic *** to potential interference from contaminants in these chromogenic substrates,their cost and potential *** substrate levels are always lower than those for saturating enzyme labels in ***,ELISA inherently has a narrow quantifiable range of an analyte of interest.
Genetic immunity is a new promising approach for the development of novel tuberculosis *** this study,it is shown that DNA vaccines expressing the fusion protein of antigen 85B (Ag85B) and antigenic target antigen MPT...
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Genetic immunity is a new promising approach for the development of novel tuberculosis *** this study,it is shown that DNA vaccines expressing the fusion protein of antigen 85B (Ag85B) and antigenic target antigen MPT83 can induce high levels of specific IgG2a antibody subtype in the mice,the ratio of antibody subtypes IgG2a to IgG1 changes 4weeks after immunization, and IgG1 is gradually shifted to the main antibody
Background As the expression of humen sperm protein 17(Sp17) in normal tissue is limited and the function is obscure,its aberrant expression in malignant tumors make it to be a candidated molecular marker for tumor im...
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Background As the expression of humen sperm protein 17(Sp17) in normal tissue is limited and the function is obscure,its aberrant expression in malignant tumors make it to be a candidated molecular marker for tumor imaging diagnosis and targeting therapy of the *** evaluate the targeting effects of anti-sperm protein 17 monoclonal antibody(anti-Sp17) on cancer in vivo and investigate its usefulness as a reagent for molecular imaging diagnosis.
Objective To develop a safe and sensitive the method of gas chromatography mass spectrometry(GC-MS) using solid phase extraction to simultaneously determine 8 pesticides(including Methamidophos,Acephate,Dimethoate,Par...
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Objective To develop a safe and sensitive the method of gas chromatography mass spectrometry(GC-MS) using solid phase extraction to simultaneously determine 8 pesticides(including Methamidophos,Acephate,Dimethoate,Parathion methyl,Malathion,Parathion,Fenvalerate,and Decamethrin) in human plasma. Methods 1mL pH6.0 phosphate buffer was added into 1mL plasma sample containing 8 pesticides.
Background & objective The role of common polymorphisms of the estrogen receptor alpha gene in cardiovascular disease was investigated in several populations,but the data regarding the relationship between ESR-a g...
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Background & objective The role of common polymorphisms of the estrogen receptor alpha gene in cardiovascular disease was investigated in several populations,but the data regarding the relationship between ESR-a gene PvuⅡsite polymorphisms and coronary artery disease(CAD) are conflicting by reported *** aim of this study was to investigate the association between the polymorphism of the estrogen receptor alpha gene PvuⅡsite and coronary heart disease in Chinese population.
Catecholamines such as dopamine and noradrenaline play important roles as neuromuscular transmitters and modulators in all parasitic helminthes,including Schistosoma *** tyrosine hydroxylase (SjTH) was amplified by ...
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Catecholamines such as dopamine and noradrenaline play important roles as neuromuscular transmitters and modulators in all parasitic helminthes,including Schistosoma *** tyrosine hydroxylase (SjTH) was amplified by rapid amplification of cDNA ends polymerase chain reaction(RACE PCR) that shows strong homology to *** H
Aims Primary biliary cirrhosis(PBC) is a progressive autoimmune liver disease characterized by the chronic inflammation and destruction of intra-hepatic small bile ducts,leading to liver fibrosis,cirrhosis,and eventua...
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Aims Primary biliary cirrhosis(PBC) is a progressive autoimmune liver disease characterized by the chronic inflammation and destruction of intra-hepatic small bile ducts,leading to liver fibrosis,cirrhosis,and eventual liver *** cellular components in human innate immune system,such as natural kill cells(NK),natural kill T cells(NKT),monocytes,macrophages,and dendritic cells(DC),represent a first-line defensive
Background Cervical cancer is one of the most common cancers *** and molecular evidence has demonstrated that high-risk human papillomaviruses(HPVs) are the main etiologic agents in the progression of cervical cancer,...
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Background Cervical cancer is one of the most common cancers *** and molecular evidence has demonstrated that high-risk human papillomaviruses(HPVs) are the main etiologic agents in the progression of cervical cancer,with at least 90%of cervical carcinomas harboring HPV DNA.E6 and E7 genes encode the major HPV *** E7 protein of high-risk HPV types disturbs cell cycle control and down-regulates components of the antigen
Objective:Chronic infection and inflammation have been regarded as one of main course of carcinogenesis and ***-like receptors(TLR) expressed on inflammatory cells play a key role in host defense against pathogens, be...
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Objective:Chronic infection and inflammation have been regarded as one of main course of carcinogenesis and ***-like receptors(TLR) expressed on inflammatory cells play a key role in host defense against pathogens, benefiting the ***,recent studies showed that TLRs were also expressed by tumor ***, the mechanisms by which TLR-triggered tumor progression remain to be fully ***
Althoughmast cells have been found in increased numbers in bronchial epithelium in asthma patients,the pathogenic role of the interaction of mast cells with bronchial epithelial cells in the development of local infla...
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Althoughmast cells have been found in increased numbers in bronchial epithelium in asthma patients,the pathogenic role of the interaction of mast cells with bronchial epithelial cells in the development of local inflammation in asthma is not well understood. In this study,primary human bronchial epithelial cells and a human mast cell line(HMC-1) were cultured either together or *** IL-6,and chemokines including CXCL1 and
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