Objectives We hypothesized that intermittent myocardial ischemia induced by exercise can promote collateral formation of coronary vessels through in- creased expression of vascular endothelial growth factor(VEGF)and i...
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Objectives We hypothesized that intermittent myocardial ischemia induced by exercise can promote collateral formation of coronary vessels through in- creased expression of vascular endothelial growth factor(VEGF)and its recep- tor fetal liver kinase-1(Flk-1). Methods A balloon constrictor was implanted on the proximal first obtuse marginal coronary artery(OM1)of Guangxi BA-MA miniature pig to establish an
Background--raumatic brain injury (TBI) has considerable morbidity and mortality,so it's necessary to further determine the long-term outcomes following inpatient rehabilitation after TBI in China,such as the qual...
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Background--raumatic brain injury (TBI) has considerable morbidity and mortality,so it's necessary to further determine the long-term outcomes following inpatient rehabilitation after TBI in China,such as the quality of life,level of function and employability,frequency of worsen outcome et al. Objective o determine the long-term outcomes of TBI and identify some fac- tors that influence *** and Results?02 patients with TBI were followed up at 2-11 years after *** two of them died and eighteen (17. 7%) had worsen *** the environmental factors,posttraumatic sei- zure influence the grade of GOS,DRS-F and DRS-E significantly as well as the score of QLI,while in our study the age only influence the latter *** all the scales we used in our study,there were significant correlation between each *** ge maybe an important factor that influence the long-term outcomes of *** further studies are required to determine if it is a seperate factor as well as age.
Objective To survey the conditions of the motor function of upper limbs improvement and cerebral functional reorganization after the constraint-in- duced therapy (CIMT therapy or CIT) in cerebral stroke *** 10 cerebra...
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Objective To survey the conditions of the motor function of upper limbs improvement and cerebral functional reorganization after the constraint-in- duced therapy (CIMT therapy or CIT) in cerebral stroke *** 10 cerebral stroke patients received 5h/d enhanced CIMT therapy and 8h/d re- strained activities of upper limbs on healthy side for 3 *** and after CIMT therapy,the flexibility of their upper limbs on affected side was assessed through WMFT,the daily use of their affected limbs was assessed through MAL,and the conditions of their cerebral functional reorganization were assessed through fMRI *** Under WMFT and MAL examination, the functional score and time score of WMFT and the quantity score and quality score of MAL were significantly different before and after CIMT therapy (P＜0. 01).Under fMRI examination,just scattered activation points existed only on focal side before CIMT therapy,and the range of activation area increased and the stimulation intensity enhanced after CIMT therapy;both activation volume and stimulation intensity in primary sensorimotor area were significantly different before and after CIMT therapy (P＜0.01);primary sensorimotor area on focal side was still the main cortical innervation area of movement at af- fected side after cerebral injury;the activation area increased and adjusted, and both auxiliary movement area and premotor area were main cortical compen- satory area after CIMT *** CIMT therapy could significantly improve the movement ability and daily use of upper limbs on affected side, and promote cerebral functional reorganization.
Embryonic stem cells are derived from inner cell mass of blastocysts. They have infinite proliferative capability and can differentiate into all types of adult cells (heart cells,skin cells,neural cells and et al.) of...
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Embryonic stem cells are derived from inner cell mass of blastocysts. They have infinite proliferative capability and can differentiate into all types of adult cells (heart cells,skin cells,neural cells and et al.) of the body under special conditions,therefore,providing unique opportunities for rehabilitation of diseases and disorders with no therapies or at best,par- tially effective ones such as cerebral ischemia,diabetes,heart attack and et ***,immune rejection can lead to the failure of human embryonic stem cell (hES cell) *** approach to address the problem is to establish hES cell line banks that have cells of various HLA *** to the limited source of human embryos and to ethical reasons,the hES cell lines are not readily *** the blastocysts that develop from those discarded D3 embryos with low morphological scores can be used as a source to establish hESC lines,the problem with availability of human embryos may be *** study was undertaken to determine whether discarded day 3 embryos with low morphological scores could develop into blastocysts and produce hES cell lines. Methods D3 embryos with low morphological scores were assessed according to Steer CV scoring system and cultured in sequential culture medium to blasto- cyst *** were evaluated by Gardner's scale and the relation between blastocyst formation rate and D3 score was investigated. (1) Trophectoderm cells were separated from the ICMs by immunosurgery and isolated ICMs were cultured for 5-8 days on mitomycin-treated mouse embryonic fibroblasts (MEF).Colonies derived from the ICMs were passed every 4-7 days and evaluated for cell surface markers including AKP,OCT-4,SSEA-4,SSEA-1, TRA-1-60,TRA-1-81,differentiation potentials and karyotypes. Results (1) A total of 19 blastocysts were obtained from 130 embryos (quality score＜ 16)and blastulation rate was 14.62%.Most of blastocysts cavitated or ex- panded on day 6.A total of 10.17% had full or ex
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