IGF-1 has the function of anti-oxidative stress and some critical patients with sepsis have a lower level of serum ***,the relationship between the IGF-1 and severity or prognosis of sepsis is still *** this paper,the...
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IGF-1 has the function of anti-oxidative stress and some critical patients with sepsis have a lower level of serum ***,the relationship between the IGF-1 and severity or prognosis of sepsis is still *** this paper,the relationship between serum IGF-1 level and the severity and prognosis of sepsis is revealed and the possible mechanism is *** clinical characteristics of patients with sepsis were recorded and *** serum IGF-1 levels and microRNA-1 levels were tested using radioimmunoassay and real-time PCR *** A549 cell line and HKC cell line were cultured in vitro and exposure to HO with or without IGF-1treatment,the cell death was detected by cell death detection ELISA kit and microRNA-1 levels were detected after HO exposure using ***-1in cells were up-regulated by transfection and IGF-1 mRNA was detected to determine its relationship with microRNA-1,moreover,the cell death was detected by cell death detection ELISA kit after *** serum IGF-1 levels in patients with sepsis were lower but the microRNA-1 levels were higher（P<0.05 *** control）.The patients in the septic shock subgroup or dead patients had the lowest IGF-1 level and the highest microRNA-1 level（P<0.05 *** and severe sepsis）.The IGF-1 level was inversely proportional to the microRNA-1 *** vitro,IGF-1could reduced the cells death caused by HO,microRNA-1 transfection couldeffectively increase the sensitivity of cells to HO damage by reducing the expression of IGF-1 which could prevent cells from injury caused by *** transfection of microRNA negative control did not influence the level of IGF-1 microRNA and the sensitivity to HO *** conclusion,the low IGF-1 level in patients with sepsis may predict serious condition and poor *** possible mechanism is that the too high microRNA-1 level reduces the IGF-1 level,making insufficient antioxidative stress of IGF-1 which increases the injury caused by oxid
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