Beta-cell apoptosis induced by adipokines may result in beta-cell dysfuction in type 2 ***,an adipokine-linked obesity with type 2 diabetes,impairs glucose-stimulated insulin secretion(GSIS)in ***,the effects of resis...
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Beta-cell apoptosis induced by adipokines may result in beta-cell dysfuction in type 2 ***,an adipokine-linked obesity with type 2 diabetes,impairs glucose-stimulated insulin secretion(GSIS)in ***,the effects of resistin on rat insulinoma cells RINm5F were *** of RINm5F with resistin induced cell *** Inhibitor of Metalloproteinase-1(TIMP-1)protected resistin-mediated cytotoxicity in *** with resistin up-regulated caspase-3 activity and induced the formation of a DNA ***-1 attenuated these *** molecular mechanism of TIMP-1 inhibition of resistin-mediated cytotoxicity appeared to involve Akt phosphorylation and activation of IκB-α*** treatment suppressed Akt phosphorylation and activated IκB-αphosphorylation,which could be attenuated by *** conclude that resistin can induce beta-cell apoptosis and that resistin-related beta-cell apoptosis can be prevented by TIMP-1.
Background To gain insight into the later stages of nephrogenesis,we detected the alterations in gene expression in rat kidney at two postnatal stages,P0(0 day after birth),the time at which nephrogensis is still acti...
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Background To gain insight into the later stages of nephrogenesis,we detected the alterations in gene expression in rat kidney at two postnatal stages,P0(0 day after birth),the time at which nephrogensis is still active,and P21(21 days after birth), when nephrogenesis is *** Sprague Dawley rats were *** were dissected from embryos at E15,E17, and E19,and from postnatal days P0,P7,P21,and adult *** subtractive hybridization(SSH)analysis was performed and highly expressed genes were evaluated as molecular markers by real-time reverse transcripfion-polymerase chain reaction (RT-PCR),in situ hybridization,and Western *** Several differentially expressed genes were identified,including rPEA3,a member of the PEA3 subfamily of Ets domain transcription *** time RT-PCR analysis revealed that rPEA3 exhibited dynamic developmental regulation,with high levels of expression from embryonic day E15 until birth,and declining levels *** in sitn hybridization,rPEA3 mRNA was detected in the ureteric bud(UB)and surrounding metanephric mesenchyme of the kidneys from E15 until birth,but was undetectable in mature *** These studies provide compelling evidence that the newly identified gene rPEA3 may play an important role in kidney development and differentiation.
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