Background Diabetic nephropathy(DN) is the leading cause of chronic kidney disease in patients starting renal replacement *** intervention and consequent therapy may prevent or at least delay the development of end-st...
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Background Diabetic nephropathy(DN) is the leading cause of chronic kidney disease in patients starting renal replacement *** intervention and consequent therapy may prevent or at least delay the development of end-stage renal *** aim of this study was to establish a proteomic method for identification of DN-related biomarkers in urine that may serve as diagnostic
Objective To primarily explore CDR3 spectratyping and molecular features of TCR |3 chain in the peripheral blood and tissue of patients with colorectal carcinoma with RQ-PCR with DNA melting curve technique. MethodsBl...
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Objective To primarily explore CDR3 spectratyping and molecular features of TCR |3 chain in the peripheral blood and tissue of patients with colorectal carcinoma with RQ-PCR with DNA melting curve technique. MethodsBlood and tissue samples were collected from seven patients with colorectal carcinoma (CRC),while blood samples were also collected from two healthy controls as *** the
Severe infection has become one of the main reasons for lower limb amputation and death in diabetes *** many broad-spectrum antibiotics are used in the clinics,the incidence of infections remains high due to the emerg...
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Severe infection has become one of the main reasons for lower limb amputation and death in diabetes *** many broad-spectrum antibiotics are used in the clinics,the incidence of infections remains high due to the emergence of resistant strains. The objective of this study was to investigate the effects of Staphylococcus aureus on the apoptosis and expression of iNOS and IL-1βin THP- 1 monocytes in the presence of high concentration of
Background Rapid and reliable determination of genetic relatedness of clinical isolates in microbiologic laboratory is essential in case of nosocomial outbreak *** present,little has been reported on the susceptibilit...
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Background Rapid and reliable determination of genetic relatedness of clinical isolates in microbiologic laboratory is essential in case of nosocomial outbreak *** present,little has been reported on the susceptibility patterns and molecular characterization of multidrug-resistant Acinetobacter baumannii clinical isolates. Methods In this study,24 *** strains isolated from nosocomial infection cases were
Objective Interleukin(IL)-27 is a new member of the IL-6/IL-12 family,composed of two subunits, the Epstein-Barr virus-induced gene 3(EBI3) and p28 chains(p28),and produced by activated monocytes and dendritic ***-27 ...
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Objective Interleukin(IL)-27 is a new member of the IL-6/IL-12 family,composed of two subunits, the Epstein-Barr virus-induced gene 3(EBI3) and p28 chains(p28),and produced by activated monocytes and dendritic ***-27 plays an important role in the regulation of differentiation of naive T helper cells and has diverse effects on innate immune ***,the pro-inflammatory mechanisms of IL-27 are still not well known.
Maple syrup urine disease(MSUD) is an autosomal recessive metabolic disorder affecting branched-chain amino *** in the BCKDHA,BCKDHB and DBT gene impair the branched-chainα-keto acid dehydrogenase complex(BCKD),resul...
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Maple syrup urine disease(MSUD) is an autosomal recessive metabolic disorder affecting branched-chain amino *** in the BCKDHA,BCKDHB and DBT gene impair the branched-chainα-keto acid dehydrogenase complex(BCKD),resulting in the accumulation of branched-chain amino acids and branched-chain a-ketoacid in tissues and *** leads to mental and physical retardation,feeding problems,
Background Asthma is a common polygenic disease,caused by complex interactions between multiple genes and environmental *** of the gene-gene interactions would contribute to a new insight into the pathogenesis and the...
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Background Asthma is a common polygenic disease,caused by complex interactions between multiple genes and environmental *** of the gene-gene interactions would contribute to a new insight into the pathogenesis and therapeutics of *** evaluate the single and combined associations
Background:25-hydroxyvitamin D(D3/D2) is frequently tested in clinical laboratories due to its importance in the management of patients with bone disease and the association between 25-hydroxyvitamin D(25(OH)D) defici...
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Background:25-hydroxyvitamin D(D3/D2) is frequently tested in clinical laboratories due to its importance in the management of patients with bone disease and the association between 25-hydroxyvitamin D(25(OH)D) deficiency/insufficiency and certain cancers,autoimmune disorders,infectious diseases,cardiovascular diseases,and many other *** methodologies for 25(OH)D testing in clinical laboratories include radioimmunoassay, enzyme-linked immunosorbent assay,high-performance liquid chromatography(HPLC),HPLC
Background:Monitoring pain management and illicit drugs/metabolites in urine is an important tool to pain management physicians in order to ensure compliance and detect drug *** spectrometry-based methods have the adv...
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Background:Monitoring pain management and illicit drugs/metabolites in urine is an important tool to pain management physicians in order to ensure compliance and detect drug *** spectrometry-based methods have the advantage of high specificity and sensitivity. Methods:200uL urine was vortex mixed with 100μL internal standard solution and 100μL limpet glucuronidase in 1M sodium acetate *** were incubated at 60℃for 16-24 hours after which 50%methanol(500μL) was *** samples were then centrifuged at 15,500 g
Aim Expression and function of inhibitor of differentiation 3(Id3) are controlled under many complex layers of *** role of Id3 on cell growth of human lung adenocarcinoma cell line(A549) remains *** aim of this study ...
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Aim Expression and function of inhibitor of differentiation 3(Id3) are controlled under many complex layers of *** role of Id3 on cell growth of human lung adenocarcinoma cell line(A549) remains *** aim of this study was to construct eukaryotic expression vector for human Id3(hId3) fused with enhanced green fluorescent protein(pEGFP/Id3),express
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