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检索条件"任意字段=中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议"

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Early Second-Trimester Serum MiRNA Profiling Predict Gestational Diabetes Mellitus

Early Second-Trimester Serum MiRNA Profiling Predict Gestati...

引用

中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Zhao Chun~(1,2),Dong Jing~3,Pan Shiyang~1,Hu Zhibi n~3,Sha Jiahao~2,Xu Junrong~1,Huang Peijun~1 1 Department of Laboratory Medicine,The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,China 2 Laboratory of Reproductive Medicine,Nanjing Medical University,210029 Nanjing,China 3 Department of Epidemiology and Biostatistics, School of public health,Nanjing Medical University,Nanjing 210029,China

Objective To systematically assess whether the serum microRNAs(miRNAs) profiling can predict gestational diabetes mellitus(GDM) in advance of the measurement of blood glucose. Methods We used systematically TaqMan Low Density Array followed by individual quantitative reverse transcription polymerase chain reaction assays to screen miRNAs in serum which collected at 16-19 gestational *** regression was

关键词： GDM miRNAs Early Second-Trimester Serum MiRNA Profiling Predict Gestational Diabetes Mellitus

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Expression of BMI-1 in human eervical tissue-a diagnostic and prognosis marker in patients with uterine cervical cancer

Expression of BMI-1 in human eervical tissue-a diagnostic an...

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中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Xin Zhang Chuanxin Wang Chengbao Zhu Jian ZHANG Shifeng Kan Lumo Du Wei Li Lili Wang Shun Wang Department of Clinical Laboratory Qilu Hospital Shandong UniversityJinan 250012China

@@ Uterine cervical cancer(UCC)remains the second most frequent malignancy in women *** infection alone is not sufficient to induce malignant changes，suggesting additional host genetic factors may be involved in the development and progression of UCC. B-cell-specific Moloney murine leukemia virusintegration site 1(ami—11 is a member ofpolycomb group，which participates in axial patteming，hematopoiesis， cell cycle regulation， and ***，overexpression of Bmi-1 has been reported in various human cancers and proved to be associated with poor *** aim of this study was to investigate the expression of Bmi-1 protein in (UCC)and explore its associations with clinicopathological factors and prognosis.

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Molecular engineering of enzymes to serve as ELISA labels for the enhanced quantification sensitivity：a future direction

Molecular engineering of enzymes to serve as ELISA labels fo...

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中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Fei LIAO Xiaolan YANG Chun ZHANG Hongbo LIU Key Laboratory of Medical Laboratory Diagnostics (the Ministry of Education)and the Department of Laboratory Medicine Chongqing Medical UniversityChongqing 400016China

@@ Enzyme-linked immunosorbent assay(ELISA) iS routinely used for the selective assay of a hapten， all antigen(Ag)，or an antibody(Ab)in specimens. The quantification sensitivity of ELISA is by no means satisfactory，*** principal external factors affecting ELISA sensitivity include the number of an enzyme conjugated to all Ab，the catalytic eHlclency ot tlae conjugate，me reacuon period of the alzyme on its substrate and the sensitivity to quantify enzyme product(s).For practical efficiency，the reaction period is *** the reactivity to all analyte of interest，the number of the enzyme conjugated to the Ab is *** catalytic efficiency of alzynle·Ab conjugates，together with the sensitivity to quantify enzyme products，should be improved primarily.

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Study on the pathogenesis of PROC P275S mutation in protein C deficiency and the role of its corresponding loop

Study on the pathogenesis of PROC P275S mutation in protein ...

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中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Tingting Yu~1,Jing Dai~2,Jian Wang~1,Xuefeng Wa ng~2,Qihua Fu~1 1 Department of Laboratory Medicine,Shanghai Children's Medical Center,Shanghai Jiaotong University School of Medicine 2 Department of Clinical Transfusion,Ruijin Hospital,Shanghai Jiaotong University School of Medicine

Background Protein C（PC） deficiency,caused by molecular genetic defects of PC gene（PROC）,has been reported to be associated with an increased risk of venous *** have identified a homo2ygous PC P275S mutation in a Chinese pedigree of type II PC deficiency.P275 is located in a loop which consists of the region from A264 to D280(Loop). Objective To study the molecular mechanisms of PC P275S mutation in PC deficiency and the role of

关键词： Study on the pathogenesis of PROC P275S mutation in protein C deficiency and the role of its corresponding loop

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A Novel Monoclonal Antibody Specific to Non-small Cell Lung Cancer Inhibits Tumor Cell Growth and Blocks Tumor Growth in A Xenograft Model

A Novel Monoclonal Antibody Specific to Non-small Cell Lung ...

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中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Pan Shiyang~(,2),Wang Fang~(,2),Xu Ting~(,2),Zhang Lixi a~(,2),Huang Peijun~(,2),Xu Jian~(,2),Zhao Chun~(,2),Xia We nying~(,2) 1 Department of Laboratory Medicine,the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road,Nanjing 210029,China 2 National Key Laboratory for Laboratory Medicine of China

Objectives We aim to prepare and characterize a monoclonal antibody(McAb) against human non-small cell lung cancer(NSCLC),and to further investigate the inhibitory effect of this McAb on the proliferation of lung adenocarcinoma cell line SPC-A1 in-vitro and in-vivo. Methods BALB/c mice were immunized with human lung adenocarcinoma cells and the spleen cells of the immunized mice were conventionally fused with SP2/0 myeloma *** resulting

关键词： NSCLC A Novel Monoclonal Antibody Specific to Non-small Cell Lung Cancer Inhibits Tumor Cell Growth and Blocks Tumor Growth in A Xenograft Model Model

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Association of Short Tandem Repeat Polymorphism in The Promoter of Prostate Cancer Gene Antigen 3 Gene With The Risk of Prostate Cancer

Association of Short Tandem Repeat Polymorphism in The Promo...

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中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Wu Zhou~1,Zhanguo Chen~1,Wangqiang Hu~1,Mo S hen~1,Xiaoxia Zhang~2,Chengdi Li~1,Zhiliang Wen~1, Xiuling Wu~1,Yuanping Hu~1,Xiaohua Zhang~1,Xi uzhi Duan~1,Xiucui Han~1,Zhihua Tao~1 1 The First Affiliated Hospital of Wenzhou Medical College 2 Ningbo Municipal Hospital of Traditional Chinese Medicine

Background:PCA3(prostate cancer gene antigen 3) gene is one of the most prostate cancer-specific genes at ***,the prostate-specific expression and the sharp up-regulation of PCA3 mRNA in prostate cancer suggest a unique transcriptional regulation,which possibly can be attributed to promoter *** our study,we evaluated whether there is polymorphism in PCA3 promoter region and we wanted to assess the association of the

关键词： Association of Short Tandem Repeat Polymorphism in The Promoter of Prostate Cancer Gene Antigen 3 Gene With The Risk of Prostate Cancer

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A single-nucleotide polymorphism of the STAT4 gene is associated with systemic lupus erythematosus(SLE) in female Chinese population

A single-nucleotide polymorphism of the STAT4 gene is associ...

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中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Haixia Luan~1,Ping Li~1,Chunwei Cao~2,Chaohua L i~2,Chaojun Hu~1,Shulan Zhang~1,Xiaofeng Zeng~1, Fengchun Zhang~1,Changqing Zeng~2,Yongzhe Li~* 1 Department of Rheumatology and Clinical Immunology,Peking Union Medical College Hospital,Peking Union Medical College & Chinese Academy of Medical Sciences,Beijing,China 2 Laboratory for personalized genomic medicine, Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing,China

Aims Systemic lupus erythematosus(SLE) (OMIM 152700) is a chronic autoimmune disease that can affect any part of the *** the detailed etiology of SLE remains elusive,it was widely accepted that genetic factors together with the environment factors contribute to the pathogenesis of SLE through abnormal immune *** disease prevalence is nine times more common in women than in men,and the

关键词： SLE A single-nucleotide polymorphism of the STAT4 gene is associated with systemic lupus erythematosus in female Chinese population

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The regulatory effect of calcineurine inhibitors on peripheral regulatory T cells and inhibitory costimulators in allo-renal recipients

The regulatory effect of calcineurine inhibitors on peripher...

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中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Yangjuan Bai~1,Yunying Shi~2,Yuangao Zou~1,Bei Cai~1,Yi Li~1,Zhuochun Huang~1,Lanlan Wang~1 1 Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,P. R.China 2 Division of Nephrology,Department of Medicine, West China Hospital,Sichuan University,Chengdu 610041,P.R.China

Objective:To explore the regulatory effect of FK506 and CsA on the expression of peripheral regulatory T cells(Tregs) and inhibitory costimulators CD152 and PD-1 on T cells and Tregs and the role of the effect in the induction and maitenance of transplant tolerance in allo-renal recipients. Method:The expression of CD4+CD25+FOXP3+Treg,CD8+CD28-Treg, CD152 and PD-1 in peripheral blood of stable allo-renal recipients receiving FK506 or CsA treatment was analyzed by ***

关键词： Tregs The regulatory effect of calcineurine inhibitors on peripheral regulatory T cells and inhibitory costimulators in allo-renal recipients

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Lower decreased serum C3 associate with microRNA146a rs2910164 heterozygote in systemic lupus erythematosus

Lower decreased serum C3 associate with microRNA146a rs29101...

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中华医学会第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： Junlong Zhang~1,Bin Yang~1,Binwu Ying~1,Dongdo ng Li~1,Yunying Shi~2,Xinbo Song~1,Bei Cai~1,Zhuo chun Huang~1,Yongkang Wu~1,Lanlan Wang~1 1 Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,P. R.China 2 Division of Nephrology,Department of Medicine, West China Hospital,Sichuan University,Chengdu 610041,P.R.China

Objectives:MicroRNAs(miRNAs) are noncoding RNAs that inhibit the expression of protein-coding genes by either translational repression or messenger RNA *** works have shown that microRNA-146a is as a negative regulator of innate immunity,and under-expression of microRNA-146a negatively correlated with clinical disease activity in *** nucleotide polymorphisms(SNPs) may significantly alter miRNA expression and *** analysis showed that G/C polymorphism of

关键词： microRNA Lower decreased serum C3 associate with microRNA146a rs2910164 heterozygote in systemic lupus erythematosus

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Setup of Hematology Quality Control Procedures with Retained Patient Samples

Setup of Hematology Quality Control Procedures with Retained...

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中华医学第九次全国检验医学学术会议暨中国医院协会临床检验管理专业委员会第六届全国临床检验实验室管理学术会议

作者： WANG HOUFANG YU JUNFENG MU YU SUN FEI Department of Laboratory Medicine Beijing United Family HospitalChina

@@ Patient blood samples constitute ideal quality control material in many *** principal challenges involve the design of a system which combines excellent performance characteristics with a minimum of extra *** study is to setup a Hematology quality control procedure with retained pmiem ***500 Hematology analyzer was monitored daily using 3 levels of 5C controls from BECKMAN·COULTER *** or threeanalyzed patient specimens were retained at 2～-8℃ per day(Day 1)，each specimen with at least one high value of WBC、RBC、HGB and PLT ***-analyze retained specimens on the second day(Day 2).Collect one momh *** WBC，RBC，MCV，HGB and PLT values were recorded_in an EXCEL *** thedifference percentage(Delta percentage.△％)ofWBC、RBC、HGB、MCV、PLT parameters according to the following equation.

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