Cotton(Gossypium)is one of the important fiber crops of the *** stress affects the nor-mal growth of cotton in every *** it is a complicated mechanism to response cold stress,RNA-Seq technology provides a new strategy...
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Cotton(Gossypium)is one of the important fiber crops of the *** stress affects the nor-mal growth of cotton in every *** it is a complicated mechanism to response cold stress,RNA-Seq technology provides a new strategy to reveal *** diploid species Gossypium thurberi and Gossypium trilobum exposed to cold stress(4℃)were used to study the cotton cold tolerance mechanisms by the next-generation RNA-Seq *** and physiological analysis proved that *** was better at regulating to adapt cold stress than *** mapped RNA-Seq data to reference genome(***),then 23 726 and 23 820 genes had been found in *** and ***,*** is more,there are 4227 and 4174 differentially expressed genes(DEGs)were found in *** and ***,***,we found signal transduction,transcription factors,and carbohydrate transport and metabolism play important roles in responding to cold stress by DEGs enrichment ***,in this study,we found that *** accumulated higher levels ofabscisic acid(ABA)than ***-mediated CBF4 signal network have been predicted by our ***2,CBF4,RAP2.7,key genes of ABA biosynthesis,that belong to CBF4 regulated network,were identified and ***,these genes expression were up-regulated because of cold *** data suggest that ABA mediated CBF4 regulated network also play a core role in ***,this study not only provides materias and a basic theory for cotton cold tolerance mechanism,but also a new idea to study abiotic stress deeply.
Upland cotton(Gossypium hirsutum L.) is one of the world's most important fiber crops,accounting for more than 90%of all cotton *** their wild progenitors have relatively short and coarse,often tan-colored fibers,...
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Upland cotton(Gossypium hirsutum L.) is one of the world's most important fiber crops,accounting for more than 90%of all cotton *** their wild progenitors have relatively short and coarse,often tan-colored fibers,modern cotton cultivars possess longer,finer,stronger,and whiter *** this study,the wild and cultivated cottons(YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis(DPA),20 DPA and mature stages at the morphological *** order to explore the effects of domestication,reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality,an iTRAQ-facilitated proteomic method was performed on developing *** were 6990 proteins identified,among them 336 were defined as differentially expressed proteins(DEPs) between fibers of wild versus domesticated *** down-or up-regulated proteins in wild cotton were involved in phenylpropanoid biosynthesis,zeatin biosynthesis,fatty acid elongation and other *** analysis between transcriptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 *** in proteomics have been verified at the mRNA level by qPCR and have been validated at the physiological and biochemical levels by POD(peroxidase) activity assays and ZA(zeatin) content *** work corroborates the major pathways involved in cotton fiber development and demonstrates that POD activity and zeatin content have a great potential related to fiber elongation and thickening.
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