Flow cytometry was used to investigated immune parameters of haemocytes in the thick shell mussel Mytiluscoruscus exposed to different salinities(15‰, 25‰ and 35‰) under two pH levels(7.3 and 8.1) for varied period...
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Flow cytometry was used to investigated immune parameters of haemocytes in the thick shell mussel Mytiluscoruscus exposed to different salinities(15‰, 25‰ and 35‰) under two pH levels(7.3 and 8.1) for varied periods using a two-way experimental approach(in vitro and in vivo). In both the experimental conditions, low p H showed significant effects on all immune parameters throughout the experiment. Total haemocyte counting(THC), phagocytosis(Pha), esterase(Est) and lysosomal content(Lyso) were significantly decreased under low p H level, whereas haemocyte mortality(HM) and reactive oxygen species(ROS) were increased under low pH level. Salinity also showed significant effects on all immune parameters of mussels, but not as strong as *** effect of high salinity on immune parameters of haemocyteswas more significant compared with low salinity. The interactive effects between pH and salinity were observed during both experimental exposure conditions in most haemocyte parameters. This study showed that high salinity, low salinity and low pH have negative and synergistic effects on haemocytes of mussels.
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