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Investigation of Rabbit Coccidial Species in Hebei Province and Identification of the Eimeria sp.n.

Investigation of Rabbit Coccidial Species in Hebei Province ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ping Cui Sufang Fang Xiaolong Gu Xianyong Liu Xun Suo College of Animal Science & Technology Hebei North University College of Veterinary Medicine China Agricultural University

In this study,coccidian species of rabbit and infection rate were investigated in Hebei *** results demonstrated that there were 13 coccidia species of rabbits in Hebei Province and the dominant species were Eimeria intestinalis(21%),***(20%),***(12%),***(11%),***(10%),***(9.5%),*** highest infection rate was 96.8% in rabbit population of 45～60 days of *** the investigation,one Eimeria strain was obtained by single-oocyst isolation *** found that the oocyst morphology is similar to that of ***,but its prepatent period is 132 h(5.5 d),much shorter than that of *** virtue of biological and molecular studies,we determined the characteristics of this *** research results included as follows: ***: Sporulated oocysts are ovoid or elongate ellipsoidal,with stieda body(SB) and sporocyst residuum(SR),without oocyst residuum(OR).The average size of the oocysts are 37.4±1.9×23.5±1.4 μm(32.6～41.2×20.9～25.5 μm),with a length(L)/width(W) ratio of *** oocyst wall is yellowish,*** micropyle is 6.2 μm( 5～7.5μm).The difference of oocyst size between this strain and *** is *** development and pathogenicity: There are 3 generations of asexual schizogony,which is differed from Eimeria irresidua(4 asexual generations).Two types of meronts(slender type and short type) were observed in each *** prepatent period is 5.5 days and patent period is 11 days with a peak of oocysts excretion on day 10 post *** with more than 3×104 sporulated oocysts could result in gross lesion in ileum and the infected rabbits were depression,piloerection and dull,decreased appetite and drinking,as well as excreting soft faeces and loose *** analysis: 18 S r DNA of this strain were sequenced and analysed by DNASTAR and aligned with corresponding sequence of the seventeen species of Eimeria in the Genbank and the phylogeneti

关键词： Rabbit Eimeria sp n Morphology Biology Endogenous development

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中草药白薇和槟榔熬制剂处理对刚地弓形虫侵染作用的影响

中草药白薇和槟榔熬制剂处理对刚地弓形虫侵染作用的影响

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者：郭海婷陈志宝黑龙江八一农垦大学生命科学技术学院

刚地弓形虫是一种重要的人兽共患性原虫,能感染包括人在内的几乎所有温血动物,对人类健康、食品卫生安全和畜牧业生产危害严重,至今仍无理想疫苗和治疗药物。中草药成分复杂,种类和炮制方法多样,可用来研发抗弓形虫感染特效药。本试验... 详细信息

刚地弓形虫是一种重要的人兽共患性原虫,能感染包括人在内的几乎所有温血动物,对人类健康、食品卫生安全和畜牧业生产危害严重,至今仍无理想疫苗和治疗药物。中草药成分复杂,种类和炮制方法多样,可用来研发抗弓形虫感染特效药。本试验选用清虚热药白薇和驱虫药槟榔药用部位,经称重、炮制、定容和除菌制备熬制剂,旨在研究其抗弓形虫侵染作用。我们首先运用MTT法探究不同浓度熬制剂对宿主Vero细胞增殖能力及其代谢的影响,确定白薇和槟榔熬制剂的适合作用范围;随后以弓形虫ME49株β-actin基因(Toxo DB:TGME4909030)为内参,采用Real Time PCR定量法考察两种熬制剂适合作用范围内各时间点(6 h、12 h、1 d、2 d和3 d)对弓形虫RH株速殖子增殖情况的影响;同时扩增Vero细胞fibrillarin(Gen Bank:JN601043)和弓形虫β-actin基因,运用琼脂糖凝胶电泳对Real Time PCR定量结果进行验证;然后按照每只BALB/c鼠每天灌胃0.16 g生药的剂量,连续给药7 d后经腹腔感染1000个RH速殖子并记录其存活时间,同时设立空白对照组,确定白薇和槟榔熬制剂能否提升BALB/c鼠抗弓形虫侵染效果;为评价两种熬制剂是否对弓形虫感染有治疗作用,腹腔注射1000个RH速殖子4 h后以每天灌胃0.16 g生药的方法连续治疗7 d并观察其存活情况,直至实验结束。结果显示,白薇和槟榔熬制剂浓度(干重)分别达到6.82mg/m L和15.54 mg/m L时,对Vero细胞毒害明显,可显著抑制宿主细胞生长(P<0.05);药物安全浓度范围内两种熬制剂(白薇:1.71 mg/m L;槟榔:1.94 mg/m L)作用3 d时,能明显抑制弓形虫RH株速殖子体外增殖(P<0.05),其他时间点均无统计学差异(P>0.05)。动物试验结果表明,白薇和槟榔熬制剂不能增强机体抗弓形虫感染作用,且对弓形虫急性感染的治疗效果不显著(P>0.05)。总之,Vero细胞对白薇较槟榔更加敏感,且两种熬制剂安全范围内均能明显抑制弓形虫体外增殖,可体内抗感染效果不显著,解析白薇和槟榔有效药物成分及其造成体内外抗弓形虫侵染能力差异的成因将成为未来研究的重点。

关键词：刚地弓形虫白薇槟榔熬制剂侵染作用

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Comparison of endogenous development of parental line and precocious line of Eimeria maxima

Comparison of endogenous development of parental line and pr...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Chaoyue Wang Chao Li Xinming Tang Dandan Hu Jingxia Suo Xun Suo Xianyong Liu National Animal Protozoa Laboratory & College of Veterinary Medicine China Agricultural University

Selection of precocious lines is successful for many Eimeria species infecting chickens and *** lines are characterized by shortened endogenous life cycle,decreased reproductive potential,attenuation of virulence and retention of ***,the difference of endogenous development of parental line and precocious line of *** is not *** we developed a precocious line of *** and compared the endogenous development with the parental *** precocious line's prepatent time was shortened from 130 h to 98 h post inoculation,which remained stable without selection *** inoculated with 5000 oocysts per bird,total oocysts output of the precocious line is reduce by 70% compared to its parental line,and the oocysts output peak occurred one day earlier in the precocious *** infected with 105 to 8×10～6 oocysts of either precocious line or parental line were killed at intervals of 8 h between 32 h and 128 h(as well as 140 h and 150 h) *** of duodenum,ileum and jejunum were processed to 5 μm *** of the HE stained sections showed that there were four generations of schizogony in parental line but just three generations in precocious *** 64 h post infection,the second generation of schizonts was observed in epithelial cells of the deep glands of the duodenum and jejunum both in parental line and precocious *** precocious line,the last generation of schizonts was found in the superficial villi and sometimes in their tips in small intestine at 80 h *** parental line developed to 4 th generation of schizonts until 112 *** result strongly suggests that one asexual stage is depleted in precocious *** was shown that the time of the transition from schizogony to gametogony in *** could be altered through pressure selection,suggesting that some of the control of this transition is inherent in the parasite.

关键词： Eimeria maxima endogenous development precocious line

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鸡柔嫩艾美尔球虫微线蛋白1(EtMIC1)抗原表位的筛选及其关键氨基酸的鉴定

鸡柔嫩艾美尔球虫微线蛋白1(EtMIC1)抗原表位的筛选及其关键氨基...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者：赵宁宁李宏梅王方昆赵孝民山东农业大学动物科技学院山东省动物生物工程与疾病防治重点实验室山东省畜禽疫病防制工程技术研究中心

鸡柔嫩艾美尔球虫微线蛋白1(EtMIC1)是微线蛋白家族的一员,属于TRAP蛋白家族。研究表明该蛋白表达于整个内生性发育阶段;参与虫体对宿主细胞的粘附,入侵,等活动,是入侵的关键蛋白;具有良好的免疫原性,可以提供较好的免疫保护力。但是该... 详细信息

鸡柔嫩艾美尔球虫微线蛋白1(EtMIC1)是微线蛋白家族的一员,属于TRAP蛋白家族。研究表明该蛋白表达于整个内生性发育阶段;参与虫体对宿主细胞的粘附,入侵,等活动,是入侵的关键蛋白;具有良好的免疫原性,可以提供较好的免疫保护力。但是该蛋白在免疫过程中起关键作用的氨基酸序列还不清楚。为了探究EtMIC1在免疫过程中起关键作用的氨基酸序列,本研究从抗原表位入手,应用实验室制备的两株抗EtMIC1单克隆抗体对EtMIC1线性B细胞表位进行筛选鉴定;表位氨基酸定点突变鉴定表位的关键氨基酸。将EtMIC1蛋白分成相互重叠的三段(EtMIC1-1-1(37-254aa);EtMIC1-1-2(248-466aa);EtMIC1-1-3(460-713aa)),以PGEX-6P-1为载体进行原核表达。诱导表达的分段GST融合短肽以抗EtMIC1单克隆抗体为一抗,通过Westernblot实验筛选阳性反应肽段;阳性反应肽段进一步分段筛选,逐步缩小表位的范围。最后分别从N端和C端进行逐个氨基酸删除,经Westernblot分析,成功筛选鉴定了两株单抗识别的精确的B细胞表位:A(N-LITFATRSK-C);H(N-ESLISAGE-C)。表位氨基酸进行逐个的丙氨酸替换,以表位对应的单克隆抗体为一抗进行Westernblot分析。结果发现任意一个表位氨基酸的改变都会使表位失去与单抗的反应原性,筛选鉴定的氨基酸序列是表位的关键氨基酸序列。分析发现,两个表位分别位于蛋白预测的胞外区和胞内区。鉴于两个表位所处位置的差异,应用间接免疫荧光技术对EtMIC1进行亚细胞定位。结果显示膜外表位(A)对应的单抗在非透化的条件下可以定位在细胞膜上;胞内表位(H)对应的单抗只有在透化的条件下才能定位在细胞膜上。这表明EtMIC1是跨膜蛋白,H表位位于膜内。本研究筛选鉴定了EtMIC1两个精确的线性B细胞表位,并对其关键氨基酸序列进行了鉴定,这是首次对EtMIC1蛋白表位鉴定的报道;间接证明EtMIC1是跨膜蛋白,为EtMIC1功能的深入研究奠定了基础。

关键词：柔嫩艾美耳球虫 EtMIC1 Western-blot 抗原表位

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额尔齐斯河复口吸虫(Diplostomum)种类研究

额尔齐斯河复口吸虫(Diplostomum)种类研究

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者：郭爱民杨昆明段成任焦丽马江霞谢志胜党瑞岳城新疆农业大学动物医学学院新疆农业大学动物科学学院

为探究额尔齐斯河复口吸虫的种类以及感染情况,本研究于2009年4月至2016年7月在额尔齐斯河(中国段)野外采样调查,通过染色观察复口吸虫的形态特征,对其特征结构进行绘图、测量及分析比较研究,并标记线粒体COI基因对复口吸虫物种分类鉴... 详细信息

为探究额尔齐斯河复口吸虫的种类以及感染情况,本研究于2009年4月至2016年7月在额尔齐斯河(中国段)野外采样调查,通过染色观察复口吸虫的形态特征,对其特征结构进行绘图、测量及分析比较研究,并标记线粒体COI基因对复口吸虫物种分类鉴定。结果表明:(1)于额尔齐斯河流域发现复口吸虫囊蚴有6种,默氏复口吸虫(***)、假尾复口吸虫(***)、匙型复口吸虫(***)、伪匙型复口吸虫(***)、棒状瘤宫吸虫(***)、瘤宫吸虫未定种(***);测量体长度(BL)等囊蚴的形态学特征结构参数16个,统计学分析方法表明形态学特征结构具有显著差异性。同时,经Gen Bank比对N1、N2分别为匙形复口吸虫、棒状瘤宫吸虫,同源性为99.4%(17/621)、97.3%(604/621),N1与伊拉克的KR271465.1和KR271440.1聚为一支,支持率99,N2与KR271475.1聚为一支,支持率99。(2)运用形态学和线粒体COI基因鉴定2种复口吸虫尾蚴,默氏复口吸虫和棒状瘤宫吸虫。测量体长度(BL)等尾蚴的形态学参数11个,统计分析得出形态学特征结构差异不显著,将试验序列与Gen Bank中BLAST网站进行分析,N4、N5默氏复口吸虫KR149525.1的虫种比对一致,同源性为99%,Gen Bank登录号KR271543.1;棒状瘤宫吸虫KR271480.1的虫种比对一致,同源性为99%,Gen Bank登录号KR272544.1。序列经Gen Bank比对、遗传距离计算和系统发育分析鉴定结果一致。此外,通过RSCU和组成偏差分析,得出复口吸虫同义密码子使用率不同,具有偏好性。额尔齐斯河地理位置特殊处于新疆维吾尔自治区北部阿勒泰地区,穿经阿尔泰山脉汇入鄂毕河。是新疆维度最高的一大水系,也为我国唯一一条北冰洋水系的河流。该流域的鱼类属于北界、全北区、围极亚区中的西伯利亚分区,正因为额尔齐斯河鱼类在世界淡水鱼类分布的独特性,可以判断该流域复口吸虫的感染分布与我国内陆水系的复口吸虫区系分布特点应该是截然不同的。复口吸虫生活史复杂,第二中间宿主鱼类在繁殖季节洄游产卵,终末宿主鸥鸟具有迁徙性,在外界环境下造成了额尔齐斯河鱼类复口吸虫区系组成与欧洲等领域寄生虫组成方面的相似性,因此,本次发现的虫种与欧洲地区复口吸虫寄生虫区系有共通性。本研究对复口吸虫进行了详细的研究,通过研究复口吸虫的形态、测定基因序列为额尔齐斯河地区复口吸虫种群的遗传特性和寄生虫区系研究提供详实的科学系资料。深入阐明额尔齐斯河北界、全北区、围极亚区、西伯利亚分区独特的地域复口吸虫区系相关科学问题,为今后的研究提供理论依据。

关键词：复口吸虫瘤宫吸虫虫种鉴定密码子偏好性系统发育分析

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转录组测序与在螨类的应用

转录组测序与在螨类的应用

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者：赵亚娥牛栋玲杨瑞胡丽西安交通大学基础医学院病原生物学与免疫学系

转录组测序是近十余年蓬勃兴起的崭新技术,不仅能够快速获得大量遗传数据进行转录本结构、变异以及非编码区域功能研究,而且对有无基因组数据物种的转录组均可进行有效注释,发现未知或低丰度转录本,更重要的是能够揭示不同状况下基因表... 详细信息

转录组测序是近十余年蓬勃兴起的崭新技术,不仅能够快速获得大量遗传数据进行转录本结构、变异以及非编码区域功能研究,而且对有无基因组数据物种的转录组均可进行有效注释,发现未知或低丰度转录本,更重要的是能够揭示不同状况下基因表达的变化,目前在节肢动物门昆虫纲和蛛形纲蜱螨亚纲蜱类已有广泛应用。但在螨类的研究却明显滞后,可能与螨虫个体微小,绝大多数不能培养,虫源获取困难有关。本文对转录组测序与在螨类的应用现状进行介绍,旨在抛砖引玉,推动螨类转录组测序的不断深入。结果显示,在自然界5万余种螨类中,检索到有转录组测序报道的仅为20种,研究对象以农业螨虫为主,包括叶螨、植绥螨、根螨、厉螨和瓦螨等,而与人和动物健康密切相关的医学螨虫仅涉及兔痒螨、腐食酪螨、犬疥螨、粉尘螨和鸡皮刺螨等。二斑叶螨是第一篇报道的个体最小的转录组测序物种,由加拿大学者于2011年发表在《Nature》上,该研究通过比较叶螨从喜食的大豆叶转移到拟南芥和番茄叶后基因表达量变化,发现CYP450、GST、CCEs等基因参与摄食与解毒,从而揭示了叶螨应对宿主变化的分子机制。2015年我国学者通过转录组测序发现泛醌-细胞色素C还原酶结合蛋白是粉尘螨引起过敏反应的一种新变应原,已被命名为Der f 24。最近,我们课题组基于转录组测序对兔痒螨的应激调节机制进行了研究,发现44℃高温组有808个基因上调,814个基因下调;-5℃低温组有769个基因上调,1530个基因下调;进一步利用生物信息学手段分析发现,主要参与应激的基因不仅包括文献报道的HSP、肌肉LIM蛋白Mlp84B、表层蛋白等基因,而且包括DFP1和DFP2类蛋白、filboin重链、肽脯氨酰顺反异构酶等新发现的基因。截止目前,有关螨类耐药、生长发育和致病等相关功能的转录组测序数据已陆续被报道,为今后这些基因的功能研究奠定了坚实的分子基础。然而,转录组测序在螨类应用仍然面临挑战:一是大部分螨虫获取困难,提取的RNA质量难以满足测序要求;二是Gen Bank中有关螨类的分子数据非常缺乏,缺乏规范统一的存放与共享数据平台;三是测序结果拼接错误时有发生,需要人工校对与验证;四是大部分研究者生物信息学知识有限,挖掘有用信息和个性化分析面临困难。但是可以期望的是,随着速度更快、精度更高及通量更高更新的单分子测序技术平台的完善和推广,螨类因个体微小、RNA提取质量不能满足测序要求等问题将得以解决,必将推动医学螨虫转录组学以及功能基因研究的快速发展,从而有效解决重要医学螨虫对人畜的危害。

关键词：螨类测序与注释转录组学功能基因

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Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes,microglia and neuron caused by Angiostrongylus cantonensis infection

Apoptosis and necroptosis of mouse hippocampal and parenchym...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhang Mengying Xu Yiyue Pan Tong Hu Yue Yanin Limpanont Huang Ping Kamolnetr Okanurak Wu Yanqi Paron Dekumyoy Zhou Hongli Dorn Watthanakulpanich Wu Zhongdao Wang Zhi Lv Zhiyue Zhongshan School of Medicine Sun Yat-sen University Key Laboratory of Tropical Disease Control (Sun Yat-sen University) Ministry of Education Faculty of Tropical Medicine Mahidol University Hunan Agriculture University

Background: Angiostrongyliasis,which is prevalent in China's mainland,the Taiwan region and Thailand and sporadic in South Africa,Brazil and Australia,is a food-borne parasite characterized by eosinophilic meningitis or *** cantonensis has been the only parasite among Angiostrongylidae to cause human central nervous system infection,which is characterized by severe headache,nuchal rigidity,and *** *** larvae cause extensive neurological impairments after invading the brains of their hosts and why paraesthesia and numbness persist long after treating *** infection with combinations of regimens and glucocorticoids remain *** death mechanisms include apoptosis,necroptosis and autophagy,which are associated with the pathogenesis of central nervous system parasitic infections,including cerebral malaria,cerebral toxoplasmosis,cerebral amebiasis,neurocysticercosis and *** aim of the present study was to investigate apoptosis,necroptosis and autophagy in the brains of mice infected with ***,which will be valuable for better understanding the pathogenesis of angiostrongyliasis ***: Functional and histological neurological impairments of brain tissues from mice infected with *** were measured by the Morris water maze test and haematoxylin and eosin(H&E) staining,*** transcriptional levels of apoptosis-related genes(caspase-2,-3,-4,-6,-9,cytochrome C,Smac,Bcl-2,Bad,and Bcl-XI),necroptosis-related genes(RIP3) and autophagy-related genes(FADD and LC3 B) were quantified by quantitative real-time polymerase chain reaction(RT-PCR).The translational levels of genes encoding caspase-3,-4,and-6,as well as cleaved caspase-3 and-6,were assessed by western blot and immunohistochemistry(IHC) *** and necroptotic cells and their distributions in infected brain tissues were analysed by flow cytometry and transmission electron microscopy(T

关键词： Angiostrongylus cantonensis pathogenesis apoptosis necroptosis

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Use of a recombinant mosquito densovirus as a gene delivery vector for the functional analysis of genes in mosquito larvae

Use of a recombinant mosquito densovirus as a gene delivery ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Pei-Wen liu Jia-Bao Xu Xiao-Guang Chen Jin-Bao Gu Department of Pathogen Biology Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical University

In vivo microinjection is the most commonly used gene transfer technique for analyzing the gene functions of an individual ***,this technique is technically demanding and involves complicated procedures,especially when used in larvae due to the small size,delicate cuticle and high death ratio,which limit its *** contrast,viral-based delivery systems have been developed to overcome extracellular and intracellular *** have the advantages of easy manipulation,higher transfection efficiency,long-term expression,and more persistent effects in *** densoviruses(MDVs) are mosquito-specific,small single-strand DNA viruses that may be an effective delivery vector for foreign nucleic acids into mosquito cells;however,because nearly all of the genome sequences are essential to viral proliferation,the replacement or insertion of foreign genes will cause the recombinant viruses to lose their packaging and/or replication abilities,which is a barrier to the development of MDVs for viral ***,we report using an artificial intronic small RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus(Aae DV) in vivo delivery system.A detailed procedure regarding the construction,packaging,and quantitative analysis of the r Aae DV vectors as well as larvae infection is *** study demonstrated the first construction of a non-defective recombinant MDV mi RNA expression system,which represents a tool for the functional analysis of mosquito genes and lays the foundation for the application of viral paratransgenesis for dengue virus *** demonstrated that Aedes albopictus 1 st instar larvae could be more easily and effectively infected by introducing the virus into the water body of the larvae breeding site,and these r Aae DVs could be used to overexpress or knock down the expression of a specific target gene in larvae,which represents a tool for the functional analysis of mosquito genes.

关键词： Mosquito densovirus gene delivery vector small RNA Aedes albopictus vector control artificial intron

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Effects of temperature on the susceptibility of Aedes albopictus to dengue virus 2

Effects of temperature on the susceptibility of Aedes albopi...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhuanzhuan Liu Zhenhong Zhang Zetian Lai Tengfei Zhou Zhirong Jia Jinbao Gu Kun Wu Xiao-Guang Chen Department of Pathogen Biology Key Laboratory of Tropical Disease Research of Guangdong ProvinceSchool of Public HealthSouthern Medical University

Dengue is a mosquito-borne disease that has been an epidemic in China for many *** albopictus is the dominant Aedes mosquito species and the main vector of dengue in ***,dengue mainly occurs in Guangdong Province;it does not occur or rarely occurs in other areas of China's *** distribution may be associated with climate,mosquito density and other factors in different regions;however,the effect of temperature on the vector competence of *** for dengue viruses remains *** this study,*** was orally infected with dengue virus 2(DENV-2) and reared at constant temperatures(18°C,23°C,28°C and 32°C) and a fluctuating temperature(28-23-18°C).The infection status of the midguts,ovaries and salivary glands of each mosquito was detected by RT-PCR at 0,5,10 and 15 days post-infection(dpi).DENV-2 RNA copies from positive tissues were quantified by q *** 18°C,DENV-2 proliferated slowly in the midgut of ***,and the virus could not spread to the salivary *** 23°C and 28°C,DENV-2 was detected in the ovaries and salivary glands at 10 *** rates of infection,dissemination,population transmission and DENV-2 copies at 28°C were higher than those at 23°C at any time *** 32°C,the extrinsic incubation period for DENV-2 in *** was only 5 dpi,and the vector competence was the highest among all the *** with 28°C,at 28-23-18°C,the positive rate and the amount of DENV-2 in the salivary glands were significantly ***,temperature is an important factor affecting the vector competence of *** for *** the suitable temperature range,the replication of DENV-2 in *** accelerated,and the extrinsic incubation period was shorter with a higher *** results provide a guide for vector control and an experimental basis for differences in the spatial distribution of dengue cases.

关键词： Aedes albopictus dengue virus 2 temperature vector competence viral titration

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Prevalence,risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu province,eastern China

Prevalence,risk factors and genetic characterization of Toxo...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhaofeng Hou Dandan Liu Shijie Su Zhenxing Zhao Jinjun Xu Chen Qian Jianping Tao College of Veterinary Medicine Yangzhou Universityand Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosesand Jiangsu Key Laboratory of Zoonosis

Toxoplasma gondii is an obligate intracellular parasitic protozoan with a worldwide *** parasites in edible tissues of pigs and oocysts from cats are the major sources of *** infection in ***,there are no data from sick pigs in veterinary clinics or from stray cats in Jiangsu province,eastern *** total,biological samples from 141 sick pigs and 64 stray cats were collected from this *** rate of *** infection in sick pigs was 46.81% using a polymerase chain reaction(PCR),and the overall prevalence of toxoplasmosis in stray cats was 34.38% by PCR and an enzyme-linked immunosorbent assay(ELISA).This indicated a widespread exposure to *** among sick pigs and stray cats in this *** toxoplasmosis was more prevalent in sick pigs in veterinary clinics than in pigs from local farms(P < 0.01).Age and geographic region were considered to be the main risk factors associated with *** infection in these *** of these,17 sick pigs and seven stray cats,were genotyped by multilocus PCR-restriction fragment length polymorphism(PCR-RFLP) with 10 genetic markers [SAG1,SAG2(5’-3’SAG2,***2),SAG3,GRA6,PK1,c22-8,c29-2,BTUB,L358 and Apico].Six distinct genotypes were found,which were designated Toxo DB PCR-RFLP genotypes #9(Chinese I),#10(Type I),#213,and #89,and New 1 and New *** I is the most prevalent *** genotype in this *** two new genotypes(designated New 1 and New 2) are reported and the Toxo DB PCR-RFLP genotype #89 is found for the first time in *** information will be useful for the prevention,diagnosis and treatment of porcine toxoplasmosis in Jiangsu province,eastern China.

关键词： Toxoplasma gondii Prevalence Risk factor Genotyping Sick pigs Stray cats

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