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检索条件"任意字段=中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会"

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Evaluation of protective immune responses induced by recombinant Trx Lp and ENO2 proteins against chronic toxoplasmosis infection in pigs

Evaluation of protective immune responses induced by recombi...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Meng Wang Nian-Zhang Zhang Xiao-Yu Yang Zhi-Jie Cai De-Lin Zhang Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Animal Husbandry and Veterinary Bureau of Liangzhou District

Toxoplasma gondii is an obligate intracellular parasitic protozoan that can infect almost all species of warm-blooded *** to that no chemical drugs could act against the tissue cyst stage of ***,vaccination may be one of ideal control *** the present study,the recombinant Tg ENO2 and Tg Trx Lp proteins were further evaluated the protective efficacy against chronic toxoplasmosis in pigs and the immune responses were also *** results showed that both of the recombinant proteins,either alone or in combination,could elicit strong humoral immune *** immunization,pigs were induced high levels of IFN-γ,IL-2,IL-12 and some other inflammatory cytokines and costimulatory molecules compared to those in mice from control *** rTg ENO2 + rTg Trx Lp also elicited increased numbers of lymphocytes,CD4+ and CD8+ T *** protection was observed in r Tg ENO2+ r Tg Trx Lp at the chronic phase of infection when compared with control six weeks after the last *** preventable fractions were 52.3%,36.2% and 20.1% in pigs immunized with r Tg ENO2 + r Tg Trx Lp,r Tg Trx Lp and r Tg ENO2 *** results of this study suggest that rTg Trx Lp and rTg ENO2,either alone or in combination,stimulated humoral and cellular immune responses,which were able to partially protect the cyst formation.

关键词： chronic toxoplasmosis pigs thioredoxin-like protein enolase2 protein vaccine protective immunity

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Selection of a Precocious Line of Eimeria magna and Biological Characteristics of it as a Precocious Vaccine

Selection of a Precocious Line of Eimeria magna and Biologic...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Sufang Fang Ping Cui Xiaolong Gu Xianyong Liu Xun Suo College of Animal Science & Technology Hebei North University College of Veterinary Medicine China Agricultural University

Coccidiosis is an important parasitic disease caused by several coccidian species in the genus of Eimeria in *** has posed a great threat to rabbit breeding industry in our *** chemotherapy has been widely used for the control of coccidiosis,alternative approaches have to be developed as more and more resistant strains have appeared and concerns of meat safety because of drug residue is *** this study,coccidian species infecting rabbits and the infection rate were investigated in Zhang Jiakou city,Hebei *** of Eimeria magna were isolated in coccidian-free rabbits using the single oocyst isolation technique,a precocious line was selected,and the biological characteristics using the precocious line as a precocious vaccine was *** research results were as follows: 1 Pathogenesis and immunogenicity of original strain(OS): The patent period and peak of oocyst excretion was consistence but oocyst output varied with inoculation dose,oocyst output was the highest at 5×10 *** with the contral group,each group presented clinic syndrome and lesions to different *** rabbit acquired nearly complete protection against 30000 oocysts of OS when the vaccination dose was 1000 oocysts of OS.2 Selection of precocious line(PL): Coccidian-free rabbits were inoculated with oocysts of 6 OS of *** and the first newly developed oocysts recovered from the intestine and stomach were used for infection of other *** prepatent period was shortened after 20 passages from 156 to 132 hours,shortened by about 24 h and remained stable after 5 passages without any selection *** feature of PL oocyst differed from that of OS(each sporocyst harboured one large refractile body instead of the two smaller ones in the OS),but difference is not significance in size;The production of OS is about 536 times higher than that of ***,Phylogenetic analysis was performed based on 18 S r DNA and ITS-1 sequence d

关键词： Rabbit Eimeria magna Selection of precocious line Vaccine

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Genotyping and virulence analysis of Toxoplasma gondii isolates from a dead human fetus and dead pigs in Jiangsu province,eastern China

Genotyping and virulence analysis of Toxoplasma gondii isola...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhaofeng Hou Yonghua Zhou Dandan Liu Shijie Su Zhenxing Zhao Jinjun Xu Chen Qian Jianping Tao College of Veterinary Medicine Yangzhou Universityand Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosesand Jiangsu Key Laboratory of Zoonosis Jiangsu Institute of Parasitic Diseases

Genetic and virulence data for Toxoplasma gondii isolates from humans and animals in China have been ***,most DNA samples used in genotyping of *** isolates were prepared from tissues of humans and animals,and *** strains used for virulence analysis were isolated mainly from *** our study,three Chinese Toxoplasma gondii isolates(one from a dead human fetus and two from dead pigs) were comfirmed as virulent strains in *** the *** isolate from the dead human fetus was identified as type I(Toxo DB #10) while the two isolates from dead pigs were type Chinese I(Toxo DB #9) with PCR-restriction fragment length polymorphism using 10 markers(SAG1,SAG2,SAG3,BTUB,GRA6,c22-8,c29-2,L358,PK1 and Apico).By cloning and sequences analysis,all isolates contained a Pvu II restriction site(572-577 bp) in the KHB fragment and five tandem repeats in the 5′ UTR region of SAG1,which were associated with *** ***,the type Chinese I isolates contained two deletions of 15 and 3 bp at positions 635 to 649 and 658 to 660,respectively,in the GRA6 *** our knowledge,this is the first report to document the presence of Chinese I genotype of *** in pigs in Jiangsu province and identify associations of sequences of the KHB fragment and SAG1 with virulence of this *** is one of the few researchs on molecular biology of type Chinese I isolates widely prevalent in China's mainland.

关键词： Toxoplasma gondii Genotyping Virulence analysis GRA6 SAG1 China

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Cell cycle events during in vitro cultivation blood stream forms of zoonotic pathogen Trypanosoma lewisi

Cell cycle events during in vitro cultivation blood stream f...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Xuan Zhang De-Hua Lai Zhao-Rong Lun Center for Parasitic Organisms State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-Sen University

It is a precisely controlled procession that one cell producing two identical daughter cells during a eukaryotic cell *** are kinds of relatively primitive eukaryotes in which includes many human and animal ***,a typical trypanosomatid protozoa contains a single nucleus and a network of mitochondrial DNA organized as "a kinetoplast".The kinetoplast is situated adjacent to the basal body of the flagellum.A cell cycle could be described by main processes: the duplication and separation of nuclei DNA and k DNA,and assemble of the new *** lewisi is a parasitic flagellate in rats(Rattus spp) distributed globally and is transmitted by *** studies concluded that *** is potentially a neglected human ***,despite of a few morphological distinct cell types were found in *** bloodstream stages,no evidence could tell which actual time point of cell cycle representing *** this research,hence,we have produced a comprehensive and quantitative description of *** cell cycle events by in vitro cultivation at 37 *** initial morphological analysis established the basic order and timing of major morphological *** DAPI staining,Ed U staining,we able to determine the division time(G2/M phase) and synthesis time(S phase) of nuclei or *** labeling was used to reveal the events of *** results showed that most of cells went through binary fission division,with a population double time of 13.3 *** addition,the nuclear and kinetoplast events were different in *** as comparison with *** and ***,which means kinetoplsat DNA replication and division is prior to the nuclei *** the G2-stage,the new flagellum emerges from the flagellar pocket and growth unattached to the old one and reaches its final size during cytokinesis until cell body is *** addition,we also shown multiple fission cells takes 3.7% of the whole cells,with multiple nuclei

关键词： Trypanosoma lewisi in vitro cell cycle flagellum growth unequal multiple fission

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Structural and functional characterization of Ss-riok-2,an RIO protein kinase encoding gene of Strongyloides stercoralis

Structural and functional characterization of Ss-riok-2,an R...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Wei-Qiang Lei Huan Zhou Jinyang Hu Ying Zhang Min Hu State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University

The atypical serine protein kinase RIOK-2 is essential for ribosome biogenesis and the cell-cycle progression in yeast and ***,there is a paucity of information about their functions in parasitic *** goal of the present work is to investigate the function of RIOK-2 protein kinase(Ss-RIOK-2) encoding gene(Ss-riok-2) from Strongyloides stercoralis,a parasitic nematode infecting humans and *** c DNA of Ss-riok-2 is 1572 bp in length,encoding a predicted protein of 523 amino acids containing a conserved N-terminal winged helix(w HTH) domain and a RIO kinase domain.A three-dimensional structure(3 D) model of ATP bound and an unbound form of Ss-RIOK-2 was generated using the Chaetomium thermophilum RIOK-2 protein kinase(Ct-RIOK-2) crystal structure 4 GYG as a template employing Modeller *** characterization identified some critical sites for ATP binding and metal *** newly binding sites potentially give opportunity to design a specific inhibitor against the Ss-RIOK-2 protein kinase and so may contribute to drug *** revealed that Ss-riok-2 is transcribed throughout all developmental stages with the highest transcript abundance in free-living females and parasitic *** putative promoter of Ss-riok-2 is 1308 bp in length and contains a number of conserved *** of transcriptional reporter expression in transgenic *** larvae indicated that Ss-RIOK-2 is expressed in intestinal *** addition,the putative promoter of Ce-riok-2,a RIOK-2 protein kinase encoding gene of the free-living nematode Caenorhabditis elegans,drives GFP expression in intestinal cells of ***,silencing of Ce-riok-2 by RNA interference caused arrested development,sterility and protruding vulva phenotype in a wild-type *** study demonstrates significant advancement in molecular and cellular biological study of *** and of parasitic nematodes generally,and provides a foundation

关键词： Strongyloides stercoralis Caenorhabditis elegans RIOK-2 GFP RNAi

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Trichinella spiralis infection suppresses collagen-induced arthritis via PD-1-mediated Th2 polarization

Trichinella spiralis infection suppresses collagen-induced a...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yuli Cheng Xing Zhu Xiaohuan Wang Xu Huyan Jingjing Huang Xinping Zhu Department of Medical Microbiology and Parasitology School of Basic Medical SciencesCapital Medical University

PD-1 is an important co-inhibitory molecule that regulates T cell tolerance and *** is evidence that helminthes exploit the PD-1 pathway to minimize excessive *** PD-1 in CD4 T cells play a major role in rheumatoid arthritis（RA）,we used the murine model to examine the role of PD-1 in CD4 T cells in effects of Trichinella spiralis infection against collagen-induced arthritis（CIA）.We observed up regulation of PD-1 in spleen CD4 T cells in mice infected with *** was suppressed in DBA/1 mice infected with ***,whereas PD-1 blockade efficiently reversed the amelioration of CIA in ***-infected *** addition,CIA mouse model infected with *** produced significantly lower levels of Th1 cytokine IFN-γ and Th17 cytokine IL-17,and higher level of Th2 cytokine IL-4,IL-5,IL-13 compared to mice without *** further used PD-1 deficient(PD-1) C57 BL/6 mice to examine the role of PD-1 in regulating CD4 T cell differentiation and proliferation during *** ***,the Th2 polarization induced by *** in wild type（WT） mice was effectively diminished in *** proliferative ability of CD4 T cells from infected WT mice was decreased compared to uninfected ***,suppressed proliferation of CD4 T-cell induced by *** infection was only marginally restored in *** study demonstrates that PD-1 plays a critical role in ***-attenuated arthritis by facilitating Th2 polarization to a greater extent than CD4 T cell hyporesponsiveness.

关键词： Trichinella spiralis Rheumatoid Arthritis PD-1 Type Ⅱ collagen CD4+ T cell

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Research in experimental infection of Yellow-Feather broilers with Histomonas meleagridis

Research in experimental infection of Yellow-Feather broiler...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhuo Zhen-nan Guo Ping Wang Zi-jing Liu Dan-dan Tao Jian-ping Xu Jin-jun Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Ministry of Education Key Lab for Avian Preventive Medicine Yangzhou University

Histomonas meleagridis(***) is a pleomorphic parasite causing histomoniasis,a common parasitic disease,which is also called infectious cecum hepatitis or blackhead *** mostly occurs in turkeys,and also is popular in chickens,guinea fowl,quail and *** is characterized by ulceration,enlargement and embolism of the cecum,white or yellow green round necrotic lesions in the *** disease often leads to a higher incidence and mortality in turkeys,causes serious economic losses,and also does some harm in the chicken *** a long time,China has been lacked of systematic and detailed study of *** study carried out the research on experimental infection of yellow-feather broilers with *** dynamic distribution of *** in yellow-feather broilers post-infection was explored.15 days old yellow-feather broilers were infected with JSYZ-A strains via the cloacal *** 7-31 days post-infection,5 chickens were slaughtered every third day and different organs and were collected and detected by PCR and pathology *** samples were also collected and analyzed at physiological and biochemical *** results showed that *** was usually detected in the cecum and liver from the 7 days post-infection and occasionally appeared in the heart,duodenum,jejunum,lung,spleen,bursa,proventriculus,and ***,it has not been found in the pancreas and brain *** persistent infection was only observed in cecum and *** liver and cecum lesions reached the biggest values at 10-16 days and decreased after 19 *** results of PCR were basically consistent with the results of pathological *** results indicated that liver and cecum were the major target organs of ***,and other organs also could be ***,the detailed route of infection remains to be further *** also lead to the weight lose in chickens and disorders of a series of rela

关键词： Histomonas meleagridis polymerase chain reaction pathology dynamic distribution biochemical indicator

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Studies on the biology of Cichlidogyrus sclerosus and the seasonal dynamics of monogenean populations and communities on three strains of tilapia in net cages

Studies on the biology of Cichlidogyrus sclerosus and the se...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ting-Bao Yang Yin-Yin Zhou School of Life Sciences Sun Yat-sen University

Tilapias are widely cultured in the world,and monogeneans are common parasitic pathogens of these fishes in *** present study on the ecology of monogeneans included the indoor experiments focusing on the egg laying and hatchment,larval infection and development mainly related to temperature,and the seasonal dynamics of monongenean communities and populations on three strains of host in the experimental cages in a small *** indoor experiments revealed that the larva of Cichlidogyrus sclerosus on the host Oreochromis niloticus reached sexual maturity in vivo on day 12 and 6 post infection at 20 ℃and 25 ℃respectively,the egg-laying peaks observed on day 23-26,13 ± 3 eggs/day at 20℃,and 14-18 days post infection,16 ± 2 eggs/day at temperature 25℃.Eggs production at 30 ℃was highest among three *** from herd infection were consistent with those by individual *** of 1 ppt can promote in vitro eggs *** low temperature(at 20℃) can prolong the lifespan of parasites in vitro and delay the egg *** for the egg hatchment,the first larvae was observed on 5-6,2,3 days,with hatching peak at 7,3,4 days at 20℃,25℃,30 ℃*** could tolerate low temperature to a certain extent although hatchment rate decreased after low temperature *** outdoor experiments on the seasonal dynamics of monogenean populations and commnunities on three strains of tilapias caged in the west lake of south campus of SYSU revealed that *** on the three strains of host had significant seasonal population variation,showing higher mean intensities and abundances in winter months than those of summer *** usually became dominant species of gill parasite communities;The *** had higher prevalences,abundances and mean intensities on the three strains of tilapias in summer seasons,and generally became dominant species of gill parasites communities in months between summer and *** longicornis had low

关键词： Tilapia Monogenean Biology Ecology

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Dynamic transcriptomes identify biogenic amines and insect-like hormonal regulation for mediating reproduction in Schistosoma japonicum

Dynamic transcriptomes identify biogenic amines and insect-l...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Jipeng Wang Ying Yu Haimo Shen Tao Qing Yuanting Zheng Qing Li Xiaojin Mo Shuqi Wang Nana Li Riyi Chai Bin Xu Mu Liu Paul J.Brindley Donald P.McManus Zheng Feng Leming Shi Wei Hu State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory of Contemporary Anthropology Collaborative Innovation Center for Genetics and DevelopmentSchool of Life SciencesFudan University National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionKey Laboratory of Parasite and Vector Biology of the Chinese Ministry of HealthWHO Collaborating Center for MalariaSchistosomiasis and Filariasis Department of Microbiology Immunology & Tropical Medicineand Research Center for the Neglected Diseases of PovertySchool of Medicine & Health SciencesGeorge Washington University

Eggs produced by the mature female parasite are responsible for the pathogenesis and transmission of *** schistosomes rely on a unique male-induced strategy to accomplish reproductive development,a process that is incompletely *** we map detailed transcriptomic profiles of male and female Schistosoma japonicum across eight time points throughout the sexual developmental process from pairing to *** dynamic gene expression pattern data reveal clear sex-related characteristics,indicative of an unambiguous functional division between males and females during their *** analysis,in situ hybridization and RNAi assays indicate that males likely use biogenic amine neurotransmitters through the nervous system to control and maintain pairing with *** addition,the analyses indicate that reproductive development of females involves an insect-like hormonal *** datasets and analyses serve as a foundation for deeper study of sexual development in this pathogen and identification of novel anti-schistosomal interventions.

关键词： Dynamic transcriptomes identify biogenic amines and insect-like hormonal regulation for mediating reproduction in Schistosoma japonicum

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Development of a new type of mosquito trapping device for Aedes mosquitoes

Development of a new type of mosquito trapping device for Ae...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Kexing Huang Yijia Guo Peiqi Zeng Xiaoguang Chen Kun Wu Key Laboratory of Prevention and Control of Emerging Infectious Diseases of Guangdong Higher Education Institutes Department of Pathogen Biology.School of Public HealthSouthern Medical University

Background:Aedes albopictus and Aedes aegyptiare mainly active in the daytime to suck blood and transmit mosquito-borne diseases such as *** present,the traditional trapping tools and monitoring methods for Aedes mosquitoes have their own shortcomings: CDC lamp-traps mainly catch Culex and Anopheles,and Mosq-ovitrapsare difficult to catch adults of Aedes,and the method using human snare raises ethical *** far there is lack of safe and effective mosquito monitoring tools in ***:We plan to develope a new type of mosquito trap for dengue vectors Aedes albopictus and Aedes aegypti,which can also be used for monitoring mosquito density and population change in ***:In our study,we combined with a variety of methods,such as adding CO2 sustained-release agent in mosquito bait as attractant,using 365 nm ultraviolet light which is sensitive to mosquito,to enhance the trapping *** design of small water plate is to simulate mosquitoes breeding and spawning *** order to achieve the mosquitoes control effect,we use the vortex airflow generated by high-powered fan to volatilize the smell of baitand to suck the mosquitos near the *** the trapped mosquitoes was used for further monitoring ***: A mosquito trapping device was successfully designed and printed out by 3 D *** experimentsmet the design requirements and field experimentsare still *** device was applied for *** : Combinedwith a variety of mosquito trapping methods,this newmosquito trapping device has high specificity and high security for monitoring and prevention of Aedes mosquito,and has wide prospect of application.

关键词： mosquito trapping device Aedes albopictus Aedes aegypti

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