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检索条件"任意字段=中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会"

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Evaluation of protective immune responses induced by recombinant Trx Lp and ENO2 proteins against chronic toxoplasmosis infection in pigs

Evaluation of protective immune responses induced by recombi...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Meng Wang Nian-Zhang Zhang Xiao-Yu Yang Zhi-Jie Cai De-Lin Zhang Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Animal Husbandry and Veterinary Bureau of Liangzhou District

Toxoplasma gondii is an obligate intracellular parasitic protozoan that can infect almost all species of warm-blooded *** to that no chemical drugs could act against the tissue cyst stage of ***,vaccination may be one of ideal control *** the present study,the recombinant Tg ENO2 and Tg Trx Lp proteins were further evaluated the protective efficacy against chronic toxoplasmosis in pigs and the immune responses were also *** results showed that both of the recombinant proteins,either alone or in combination,could elicit strong humoral immune *** immunization,pigs were induced high levels of IFN-γ,IL-2,IL-12 and some other inflammatory cytokines and costimulatory molecules compared to those in mice from control *** rTg ENO2 + rTg Trx Lp also elicited increased numbers of lymphocytes,CD4+ and CD8+ T *** protection was observed in r Tg ENO2+ r Tg Trx Lp at the chronic phase of infection when compared with control six weeks after the last *** preventable fractions were 52.3%,36.2% and 20.1% in pigs immunized with r Tg ENO2 + r Tg Trx Lp,r Tg Trx Lp and r Tg ENO2 *** results of this study suggest that rTg Trx Lp and rTg ENO2,either alone or in combination,stimulated humoral and cellular immune responses,which were able to partially protect the cyst formation.

关键词： chronic toxoplasmosis pigs thioredoxin-like protein enolase2 protein vaccine protective immunity

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Molecular identification and determination of the nearly complete mitochondrial genome sequences of two trematodes of birds of Pakistan

Molecular identification and determination of the nearly com...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Suleman Nehaz Muhammad Jun-Jun Ma Miao-Miao Sun Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences

The accurate identification and characterization of any parasites is of great importance for any parasitological *** digenetic trematodes are an important group of parasites of vertebrates including birds and thus need a practical attempt concerning their life-histories,adult morphology and molecular *** specimens of Lyperosomum collurionis Skrjabin and Isaichikov,1927(Dicrocoeliidae) and four specimens of Uvitellina indica Siddiqi and Jairajpuri,1962(Cyclocoeliida) were recovered from the liver of Rufous treepie(Dendrocitta vagabunda) and Red-wattled lapwing(Vanellus indicus),*** mounted slides of two specimens of each species were prepared for detail morphological examination and the remaining samples were used for molecular *** or nearly complete sequences of large(LSU) and small(SSU) subunit r DNA(18 S and 28 S r DNA) as well as the internal transcribed spacers(ITS) of the nuclear r DNA gene were determined and annotated for each *** analysis of 18 S r DNA(1854 bp) and 28 S r DNA(1327 bp) for *** revealed 99% similarities with available sequences of these genes for the mentioned species,recovered from Sylvia atricapilla(Blackcap) with accession numbers AY222143 and AY222259,*** 18 S r DNA and 28 S r DNA sequences of *** show 97% and 90% similarities,respectively with that of Cyclocoelum mutabile;the member of another genus of the family *** sequences of ITS region for each recovered species will be uploaded to NCBI for the first *** complete mitochondrial genome sequences(from partial Cox3 to partial NAD5) of the two species are amplified and sequenced while the remaining region will be amplified very *** is the first attempt to describe molecular identification and determination of the nearly complete mitochondrial genome sequences of two trematodes of birds of Pakistan

关键词： Trematodes Lyperosomum Uvitellina Birds Pakistan

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Selection of a Precocious Line of Eimeria magna and Biological Characteristics of it as a Precocious Vaccine

Selection of a Precocious Line of Eimeria magna and Biologic...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Sufang Fang Ping Cui Xiaolong Gu Xianyong Liu Xun Suo College of Animal Science & Technology Hebei North University College of Veterinary Medicine China Agricultural University

Coccidiosis is an important parasitic disease caused by several coccidian species in the genus of Eimeria in *** has posed a great threat to rabbit breeding industry in our *** chemotherapy has been widely used for the control of coccidiosis,alternative approaches have to be developed as more and more resistant strains have appeared and concerns of meat safety because of drug residue is *** this study,coccidian species infecting rabbits and the infection rate were investigated in Zhang Jiakou city,Hebei *** of Eimeria magna were isolated in coccidian-free rabbits using the single oocyst isolation technique,a precocious line was selected,and the biological characteristics using the precocious line as a precocious vaccine was *** research results were as follows: 1 Pathogenesis and immunogenicity of original strain(OS): The patent period and peak of oocyst excretion was consistence but oocyst output varied with inoculation dose,oocyst output was the highest at 5×10 *** with the contral group,each group presented clinic syndrome and lesions to different *** rabbit acquired nearly complete protection against 30000 oocysts of OS when the vaccination dose was 1000 oocysts of OS.2 Selection of precocious line(PL): Coccidian-free rabbits were inoculated with oocysts of 6 OS of *** and the first newly developed oocysts recovered from the intestine and stomach were used for infection of other *** prepatent period was shortened after 20 passages from 156 to 132 hours,shortened by about 24 h and remained stable after 5 passages without any selection *** feature of PL oocyst differed from that of OS(each sporocyst harboured one large refractile body instead of the two smaller ones in the OS),but difference is not significance in size;The production of OS is about 536 times higher than that of ***,Phylogenetic analysis was performed based on 18 S r DNA and ITS-1 sequence d

关键词： Rabbit Eimeria magna Selection of precocious line Vaccine

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Genotyping and virulence analysis of Toxoplasma gondii isolates from a dead human fetus and dead pigs in Jiangsu province,eastern China

Genotyping and virulence analysis of Toxoplasma gondii isola...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhaofeng Hou Yonghua Zhou Dandan Liu Shijie Su Zhenxing Zhao Jinjun Xu Chen Qian Jianping Tao College of Veterinary Medicine Yangzhou Universityand Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosesand Jiangsu Key Laboratory of Zoonosis Jiangsu Institute of Parasitic Diseases

Genetic and virulence data for Toxoplasma gondii isolates from humans and animals in China have been ***,most DNA samples used in genotyping of *** isolates were prepared from tissues of humans and animals,and *** strains used for virulence analysis were isolated mainly from *** our study,three Chinese Toxoplasma gondii isolates(one from a dead human fetus and two from dead pigs) were comfirmed as virulent strains in *** the *** isolate from the dead human fetus was identified as type I(Toxo DB #10) while the two isolates from dead pigs were type Chinese I(Toxo DB #9) with PCR-restriction fragment length polymorphism using 10 markers(SAG1,SAG2,SAG3,BTUB,GRA6,c22-8,c29-2,L358,PK1 and Apico).By cloning and sequences analysis,all isolates contained a Pvu II restriction site(572-577 bp) in the KHB fragment and five tandem repeats in the 5′ UTR region of SAG1,which were associated with *** ***,the type Chinese I isolates contained two deletions of 15 and 3 bp at positions 635 to 649 and 658 to 660,respectively,in the GRA6 *** our knowledge,this is the first report to document the presence of Chinese I genotype of *** in pigs in Jiangsu province and identify associations of sequences of the KHB fragment and SAG1 with virulence of this *** is one of the few researchs on molecular biology of type Chinese I isolates widely prevalent in China's mainland.

关键词： Toxoplasma gondii Genotyping Virulence analysis GRA6 SAG1 China

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The Metabolic Profile of Aedes albopictus with Deltamethrin Resistanceby 1H-NMR-based Metabolomics

The Metabolic Profile of Aedes albopictus with Deltamethrin ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Lianfen Huang Ruili Xie Lilan Peng Xiaoting Luo Yao Zhou Xiaoguang Chen Hua Li Department of Pathogen Biology and Experimental teaching center of Preventive Medicine Guangdong Provincial Key Laboratory of Tropical DiseaseSchool of Public HealthSouthern Medical University

Insecticide resistance of Aedes albopictus presents a global threat to vector borne disease like Dengue and Zika virus *** common resistance mechanism for mosquito is by adapting its physiology and metabolism for its survival in the presence of insecticide,and the metabolomic characterization could reveal biochemical fingerprints in correspondence to specific ***: To understand the adaptive metabolic changes and seek for differential metabolic biomarkers and pathways in accordance with the resistance phenotype alterations in Aedes albopictus with deltamethrin ***: Themetabolites in Aedes albopictus were detect by 1 hydrogen-nuclear magnetic resonance spectroscopy(1 H-NMR),and the comparative 1 H-NMR metabolomic analysis was performed on a laboratory deltamethrin-resistant strain(DR) with acquired resistance to deltamethrin and its parental deltamethrin-susceptible strain(DS) at the stage of both larva and adult in Aedes albopictus,followed by multivariate statistical analysis with the orthogonal pattern recognition methods(PCA,PLS-DA and OPLS-DA).The metabolic pathway analysis was performed by metaboanalyst version 3.0 and KEGG metabolic pathway ***: A total of 61 metabolites,including 25 amino acids and their derivatives,5 amines and ammoniums compounds,12 organic acids,6 sugars,9 nucleic acid components and 4 other metabolites were detected in Aedes *** separation of DS and DR phenotypic metabolic profiles was found at both larval and adult *** were 9 differential metabolites(VIP value>1 &P<0.05) in larvae,including glucose,maltose,lactate,tau-Mehis,Arg,Ser,Ala,Tyr and 2-hydroxuglutin,and another 9 differential metabolites in adults,including sucrose,maltose,trehalose,lactate,Pro,Ala,Glu,Lys and *** were 17 differential pathways( impact value>0.10) were found at both larval and adult stage,such as Trp metabolism,β-Ala metabolism,Phe metabolism,***,the Tyr metabolism pa

关键词： Aedesalbopictus Deltamethrin Resistance NMR Metabolomics

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Structural and functional characterization of Ss-riok-2,an RIO protein kinase encoding gene of Strongyloides stercoralis

Structural and functional characterization of Ss-riok-2,an R...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Wei-Qiang Lei Huan Zhou Jinyang Hu Ying Zhang Min Hu State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural University

The atypical serine protein kinase RIOK-2 is essential for ribosome biogenesis and the cell-cycle progression in yeast and ***,there is a paucity of information about their functions in parasitic *** goal of the present work is to investigate the function of RIOK-2 protein kinase(Ss-RIOK-2) encoding gene(Ss-riok-2) from Strongyloides stercoralis,a parasitic nematode infecting humans and *** c DNA of Ss-riok-2 is 1572 bp in length,encoding a predicted protein of 523 amino acids containing a conserved N-terminal winged helix(w HTH) domain and a RIO kinase domain.A three-dimensional structure(3 D) model of ATP bound and an unbound form of Ss-RIOK-2 was generated using the Chaetomium thermophilum RIOK-2 protein kinase(Ct-RIOK-2) crystal structure 4 GYG as a template employing Modeller *** characterization identified some critical sites for ATP binding and metal *** newly binding sites potentially give opportunity to design a specific inhibitor against the Ss-RIOK-2 protein kinase and so may contribute to drug *** revealed that Ss-riok-2 is transcribed throughout all developmental stages with the highest transcript abundance in free-living females and parasitic *** putative promoter of Ss-riok-2 is 1308 bp in length and contains a number of conserved *** of transcriptional reporter expression in transgenic *** larvae indicated that Ss-RIOK-2 is expressed in intestinal *** addition,the putative promoter of Ce-riok-2,a RIOK-2 protein kinase encoding gene of the free-living nematode Caenorhabditis elegans,drives GFP expression in intestinal cells of ***,silencing of Ce-riok-2 by RNA interference caused arrested development,sterility and protruding vulva phenotype in a wild-type *** study demonstrates significant advancement in molecular and cellular biological study of *** and of parasitic nematodes generally,and provides a foundation

关键词： Strongyloides stercoralis Caenorhabditis elegans RIOK-2 GFP RNAi

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Cell cycle events during in vitro cultivation blood stream forms of zoonotic pathogen Trypanosoma lewisi

Cell cycle events during in vitro cultivation blood stream f...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Xuan Zhang De-Hua Lai Zhao-Rong Lun Center for Parasitic Organisms State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-Sen University

It is a precisely controlled procession that one cell producing two identical daughter cells during a eukaryotic cell *** are kinds of relatively primitive eukaryotes in which includes many human and animal ***,a typical trypanosomatid protozoa contains a single nucleus and a network of mitochondrial DNA organized as "a kinetoplast".The kinetoplast is situated adjacent to the basal body of the flagellum.A cell cycle could be described by main processes: the duplication and separation of nuclei DNA and k DNA,and assemble of the new *** lewisi is a parasitic flagellate in rats(Rattus spp) distributed globally and is transmitted by *** studies concluded that *** is potentially a neglected human ***,despite of a few morphological distinct cell types were found in *** bloodstream stages,no evidence could tell which actual time point of cell cycle representing *** this research,hence,we have produced a comprehensive and quantitative description of *** cell cycle events by in vitro cultivation at 37 *** initial morphological analysis established the basic order and timing of major morphological *** DAPI staining,Ed U staining,we able to determine the division time(G2/M phase) and synthesis time(S phase) of nuclei or *** labeling was used to reveal the events of *** results showed that most of cells went through binary fission division,with a population double time of 13.3 *** addition,the nuclear and kinetoplast events were different in *** as comparison with *** and ***,which means kinetoplsat DNA replication and division is prior to the nuclei *** the G2-stage,the new flagellum emerges from the flagellar pocket and growth unattached to the old one and reaches its final size during cytokinesis until cell body is *** addition,we also shown multiple fission cells takes 3.7% of the whole cells,with multiple nuclei

关键词： Trypanosoma lewisi in vitro cell cycle flagellum growth unequal multiple fission

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Trichinella spiralis infection suppresses collagen-induced arthritis via PD-1-mediated Th2 polarization

Trichinella spiralis infection suppresses collagen-induced a...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yuli Cheng Xing Zhu Xiaohuan Wang Xu Huyan Jingjing Huang Xinping Zhu Department of Medical Microbiology and Parasitology School of Basic Medical SciencesCapital Medical University

PD-1 is an important co-inhibitory molecule that regulates T cell tolerance and *** is evidence that helminthes exploit the PD-1 pathway to minimize excessive *** PD-1 in CD4 T cells play a major role in rheumatoid arthritis（RA）,we used the murine model to examine the role of PD-1 in CD4 T cells in effects of Trichinella spiralis infection against collagen-induced arthritis（CIA）.We observed up regulation of PD-1 in spleen CD4 T cells in mice infected with *** was suppressed in DBA/1 mice infected with ***,whereas PD-1 blockade efficiently reversed the amelioration of CIA in ***-infected *** addition,CIA mouse model infected with *** produced significantly lower levels of Th1 cytokine IFN-γ and Th17 cytokine IL-17,and higher level of Th2 cytokine IL-4,IL-5,IL-13 compared to mice without *** further used PD-1 deficient(PD-1) C57 BL/6 mice to examine the role of PD-1 in regulating CD4 T cell differentiation and proliferation during *** ***,the Th2 polarization induced by *** in wild type（WT） mice was effectively diminished in *** proliferative ability of CD4 T cells from infected WT mice was decreased compared to uninfected ***,suppressed proliferation of CD4 T-cell induced by *** infection was only marginally restored in *** study demonstrates that PD-1 plays a critical role in ***-attenuated arthritis by facilitating Th2 polarization to a greater extent than CD4 T cell hyporesponsiveness.

关键词： Trichinella spiralis Rheumatoid Arthritis PD-1 Type Ⅱ collagen CD4+ T cell

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Identification and characterization of Eimeria tenella autophagy marker protein ATG8

Identification and characterization of Eimeria tenella autop...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Qi Nanshan Liao Shenquan Li Juan Lv Minna Wu Caiyan Lin Xuhui Hu Junjing Yu Linzeng Liu Baozhen Xiao Wenwan Sun Mingfei Li Guoqing College of Veterinary Medicine South China Agricultural University Institute of Animal Health Guangdong Academy of Agricultural Sciences

Autophagy is a "self-eating" process in eukaryotic cells involved in the targeted degradation of damaged or senescent cells,organelles,denatured proteins,nucleic acids and other biological macromolecules to maintain the cell *** now,about 35 conserved autophagy-related genes have been identified in yeast and mammalian study,but there were no reports about autophagy study in Eimeria *** our early study,about 11 orthologs of autophagy genes were found in the *** genome database,including the autophagy marker atg8,which is conserved from apicomplexan to *** this study,Etatg8,a 375 bp open reading frame encoding a protein of 124 amino acids was identified from ***,which was highly conserved from Eimeria to *** q RT-PCR and western blotting results revealed the Etatg8 gene was expressed in all four developmental stages(unsporulated oocysts,sporulated oocysts,sporozoites and second-generation merozoites),but the transcript level was significant higher at unsporulated oocyst stage than other three *** immunofluorescence assay(IFA) showed that the Et ATG8 was generally distributed throughout the cytosol of sporozoites and merozoites in complete Dulbecco's modified Eagle medium(DMEM).After incubation in Hank's balanced salt solution(HBSS) or DMEM with autophagy inducer of rapamycin or monencin,the sporozoites and merozoites displayed one and more punctuate Et ATG8 positive signal in the apical ***,our results suggest that Et ATG8 was an autophagy marker protein,which might play an important role in the autophagy of ***.

关键词： Eimeria tenella Autophagy related gene atg8 Cloning and expression qRT-PCR and western blotting Immunofluorescence assay

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Research in experimental infection of Yellow-Feather broilers with Histomonas meleagridis

Research in experimental infection of Yellow-Feather broiler...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Zhuo Zhen-nan Guo Ping Wang Zi-jing Liu Dan-dan Tao Jian-ping Xu Jin-jun Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Ministry of Education Key Lab for Avian Preventive Medicine Yangzhou University

Histomonas meleagridis(***) is a pleomorphic parasite causing histomoniasis,a common parasitic disease,which is also called infectious cecum hepatitis or blackhead *** mostly occurs in turkeys,and also is popular in chickens,guinea fowl,quail and *** is characterized by ulceration,enlargement and embolism of the cecum,white or yellow green round necrotic lesions in the *** disease often leads to a higher incidence and mortality in turkeys,causes serious economic losses,and also does some harm in the chicken *** a long time,China has been lacked of systematic and detailed study of *** study carried out the research on experimental infection of yellow-feather broilers with *** dynamic distribution of *** in yellow-feather broilers post-infection was explored.15 days old yellow-feather broilers were infected with JSYZ-A strains via the cloacal *** 7-31 days post-infection,5 chickens were slaughtered every third day and different organs and were collected and detected by PCR and pathology *** samples were also collected and analyzed at physiological and biochemical *** results showed that *** was usually detected in the cecum and liver from the 7 days post-infection and occasionally appeared in the heart,duodenum,jejunum,lung,spleen,bursa,proventriculus,and ***,it has not been found in the pancreas and brain *** persistent infection was only observed in cecum and *** liver and cecum lesions reached the biggest values at 10-16 days and decreased after 19 *** results of PCR were basically consistent with the results of pathological *** results indicated that liver and cecum were the major target organs of ***,and other organs also could be ***,the detailed route of infection remains to be further *** also lead to the weight lose in chickens and disorders of a series of rela

关键词： Histomonas meleagridis polymerase chain reaction pathology dynamic distribution biochemical indicator

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