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检索条件"任意字段=中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会"

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Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus

Characterization of a novel aspartyl protease inhibitor from...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Baojie Li Lixin Xu Xiaokai Song Xiangrui Li Ruofeng Yan College of Veterinary Medicine Nanjing Agricultural University

Background: Aspartyl protease inhibitor(API) was thought to protect intestinal parasitic nematodes from their hostile proteolytic *** in Ostertagia ostertagi,Ascaris suum and Brugia malayi indicated that Aspins might play roles in nematode *** a recent study,proteins differential expressed between free-living third-stage larvae(L3) and activated L3(x L3) of Haemonchus contortus were identified by 2 *** was found down-regulated in x L3 when compared with ***,there was no report about the functions of *** API in the parasite-host *** this study,the gene encoding API from *** was cloned,expressed,and partial of the biological characteristics were ***: A pair of gene specific primers was designed based on the API sequence of ***,and the ORF encoding API was amplified by RT-PCR,followed by cloning into p MD18-T vector,sequencing and *** ORF was sub-cloned into prokaryotic expression vector p ET-32 a(+),the recombined protein was induced by IPTG and purified by Ni-NTA *** serum against the recombinant protein was produced to develop Western blot and to study the localization of the native API in adult worm *** transcriptions of API in different stages(egg,L3,x L3,female and male adult worms) were tested by quantitative RT-PCR(q RT-PCR).The efficiency of the recombinant API in inhibiting pepsin's activity was assayed in *** evaluate the effects of recombinant API on cytokines expression,goat peripheral blood mononuclear cells(PBMCs) were incubated with the recombinant *** m RNAs encoding IL-2,IL-4,IL-10,IL-17,IFN-γ and TGF-β were quantified by q ***: In this study,a DNA fragment of 681 bp was amplified by *** one percent of the amino acid sequence was similar with the Aspin from Ostertagia *** recombinant API protein was fusion expressed with the molecular weight of 48×*** of Western blot showed that

关键词： Haemonchus contortus Aspartyl protease inhibitor Localization Differential expression Inhibitory activity Induction of cytokines

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Dermacentor nuttalli with Gene polymorphism in Inner Mongolia had potential risk to transmit the human and animal brucellosis

Dermacentor nuttalli with Gene polymorphism in Inner Mongoli...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Tianpeng Huang Mi Wu Geriletu Inner mongolia agricultural university

Background: Dermacentor nuttalli(***)is a common tick species in Inner ***,outbreak of brucellosis increases *** ticks carry a variety of pathogens that can spread a wide range of animal *** epidemic may be related to the increased activity of ticks and other *** objective of this study were to determine gene polymorphism from the same area in Inner Mongolia and to investigate the relationship between *** and Brucella specific genes,besides,to assess the potential risk of *** can be a medium to transmit the brucellosis to animals,even ***:1500 tick samples were collected from 15 pastoral sites in the Hulun Buir area of Inner Mongolia during *** species were identified through the phylogenetic tree and gene polymorphism analyses of 16 S r RNA and ITS2 *** the brucellosis pathogen detection,DNA was extracted from ticks at different developmental stages and Brucella specific genes were amplified by standard and Taq Man Real-Time ***:1500 collected ticks were *** showing significant polymorphisms with sequence differences up to 1.7%.Standard PCR analysis of Brucella specific genes showed that a minimum of 50% of tick samples were positive,even up to 90% in some sampling *** them,50 positive and 50 negative samples were randomly selected for the quantification by Real-Time ***,the most copies was 2.44×105(ct 23.67) and 16.39(ct 37.07) at *** up,*** showing genetic polymorphisms is a common tick in the Hulun Buir area of Inner *** PCR showed the presence of Brucella specific genes at all developmental ***:This finding demonstrated several gene polymorphism in ticks from the same area,and analyzed the reason of which might be the ticks in order to adopt to the various environment to show gene *** the meanwhile,it also revealed *** can transmit Brucella vertically and carry bacteria for a long ti

关键词： Dermacentor nuttalli Gene polymorphism Brucella specific genes brucellosis tick borne diseases

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Molecular characterization of calmodulin from Cryptocaryon irritans

Molecular characterization of calmodulin from Cryptocaryon i...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Wei Ni Xiaohong Huang College of Life Sciences Fujian Normal University

Cryptocaryon irritans is a kind of obligatory parasitic ciliate,which can cause the so-called white spot disease on marine *** disastrous outbreak of *** often results in huge financial losses to *** this study,we focused on the molecular characterization and function analysis of Ca M gene getting from the full-length c DNA library of *** trophonts,so as to gain a better understanding of Ci Ca M's role in the development of *** full-length c DNA of Ci Ca M gene is 540 bp,containing an open reading frame（ORF） of 450 bp,which encodes a polypeptide of 16.9 k Da,consisting of 149 amino *** order to make it available for eukaryotic expression,the non-universal codons in the original ORF of Ci Ca M gene has been modified into universal codons by artificial synthesis,followed by subcloning into pGEX-4 T-1 and transforming into *** *** recombinant plasmid was cultured in auto-induced medium ZYM-5052 to express the fusion protein,which was then purified by glutathione sepharose 4 B,followed by the cleavage of GST tag with ***-PAGE was then performed to analyze the molecular mass of the purified *** result of SDS-PAGE showed that the molecular masses of GST-rCiCaM and rCiCaM were 43 k Da and 16.9 k Da respectively,which corresponded with the predicted *** reverse-transcription PCR and western blotting analysis showed that Ci Ca M gene was expressed in all developmental stages of *** at both m RNA and protein *** localization of native Ci Ca M protein by immunofluorescent antibody test showed that Ci Ca M was dispensed all over the cytosol of *** theront,especially abundant around the four large nuclei and the peripheral area of *** overlay assay and Pull-down assay were both used to see if possible interaction between rCiCaM and rCiADF,rCiCaM and rCiProfilin could *** it turned out that rCiCaM could interact with rCiADF in a Ca-dependent way,but no such inte

关键词： Ca2+ calmodulin cloning expression actin depolymerization factor profilin

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柔嫩艾美耳球虫子孢子酵母双杂交cDNA文库的构建及钙依赖蛋白激酶3相互作用蛋白的筛选

柔嫩艾美耳球虫子孢子酵母双杂交cDNA文库的构建及钙依赖蛋白激酶...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者：徐帅兵黄兵赵其平董辉朱顺海吕凌陈婷赵宗平严茗韩红玉中国农业科学院上海兽医研究所农业部动物寄生虫学重点实验室上海师范大学生命与环境科学学院

钙依赖蛋白激酶(Calcium dependent protein kinases,CDPKs)作为多基因编码的大家族中的成员,通过Ca2+信号通路向下游传递磷酸化信号而产生级联放大效应,从而使相关蛋白发挥功能。本实验室前期对EtCDPK3基因特性进行了研究,结果表明EtCD... 详细信息

钙依赖蛋白激酶(Calcium dependent protein kinases,CDPKs)作为多基因编码的大家族中的成员,通过Ca2+信号通路向下游传递磷酸化信号而产生级联放大效应,从而使相关蛋白发挥功能。本实验室前期对EtCDPK3基因特性进行了研究,结果表明EtCDPK3蛋白在球虫子孢子阶段高表达,在虫体入侵和逸出宿主细胞过程中发挥一定的作用。为了筛选EtCDPK3的作用底物,本研究构建了柔嫩艾美耳球虫子孢子酵母双杂交cDNA文库,以EtCDPK3为诱饵构建重组质粒pGBKT7-EtCDPK3,对子孢子cDNA文库进行筛选,并对筛选阳性克隆的EST序列进行Blast比对和生物信息学分析,筛选到11个阳性质粒。随机挑取3个阳性质粒(编号:Et12、Et14、Et27)进行回复杂交验证,发现在高度缺陷性培养基上有蓝色单克隆长出,推测这些阳性质粒与诱饵质粒之间可能存在互作关系。为了进一步验证它们的关系,我们用PCR技术扩增了3个基因的ORF序列,并成功构建了用于BiFC系统的真核重组表达质粒pBiFC-VC155-Et12、pBiFC-VC155-Et14、pBiFC-VC155-Et27,同时将EtCDPK3连接到pBiFC-VN155载体构建了重组质粒pBiFC-VN155-EtCDPK3,将鉴定正确的3个阳性重组质粒分别与pBiFC-VN155-EtCDPK3共转染到DF-1细胞中,倒置荧光显微镜下观察,结果显示除阳性对照有绿色荧光以外,阴性对照和样品组均没有绿色荧光存在;同时又构建了真核重组质粒pcDNA3.1-flag-Et12、pcDNA3.1-flag-Et14、pcDNA3.1-flag-Et27与pcDNA3.1-EtCDPK3,间接免疫荧光和Westernblot的结果表明重组质粒在DF-1细胞中能够成功表达。经免疫共沉淀实验验证,结果表明Flag标签单抗可以沉淀Et12、Et14、Et27表达的蛋白,但是不能沉淀EtCDPK3表达的蛋白。上述实验结果表明,Et12、Et14、Et27与EtCDPK3可能不存在相互作用关系。为了进一步验证EtCHP12的功能,PCR扩增了EtCHP12基因的ORF全长,连接到原核表达载体pET-28a成功构建了原核重组表达质粒pET-28a-EtCHP12,并在大肠杆菌BL21中成功表达,获得可溶性的重组蛋白。将纯化的重组蛋白免疫新西兰大白兔制备多克隆抗血清。体外抑制实验结果表明,纯化后的抗rEtCHP12多抗作用子孢子后,与正常兔血清IgG组相比子孢子入侵DF-1细胞的能力明显下降,且随着抗体浓度的不断增大,子孢子入侵力逐渐降低,抑制率逐渐上升,当抗体浓度为100μg/mL,抑制率高达60%。说明EtCHP12蛋白在子孢子入侵过程中发挥了重要的作用。

关键词：球虫 Ca2+信号通路钙依赖蛋白激酶作用蛋白

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中国野生猕猴感染的诺氏内阿米巴虫株的遗传多样性的研究

中国野生猕猴感染的诺氏内阿米巴虫株的遗传多样性的研究

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者：卫苗冯萌管悦周航付永锋蔡俊龙程训佳复旦大学基础医学院病原生物学系

目的诺氏内阿米巴是非人灵长目动物流行的内阿米巴虫株。对我国广西、贵州和四川部分地区野生猕猴的内阿米巴流行病学调查发现,诺氏内阿米巴感染率为16%至62%。前期的研究发现诺氏内阿米巴虫株遗传多样性和地理位置有关,但同时也发现同... 详细信息

目的诺氏内阿米巴是非人灵长目动物流行的内阿米巴虫株。对我国广西、贵州和四川部分地区野生猕猴的内阿米巴流行病学调查发现,诺氏内阿米巴感染率为16%至62%。前期的研究发现诺氏内阿米巴虫株遗传多样性和地理位置有关,但同时也发现同一地区分离的诺氏内阿米巴虫株基因型不同的现象,提示可能存在其他因素影响诺氏内阿米巴遗传多样性。本实验调查了黄山藏酋猴内阿米巴流行情况,合并其他地区收集的样本,分析了诺氏内阿米巴遗传多样性的可能影响因素。方法从中国安徽黄山共收集60份野生藏酋猴的粪便样品。粪便经包囊富集后镜检。同时从粪便中的提取基因组DNA后,分别以特异性引物扩增溶组织内阿米巴、迪斯帕内阿米巴、诺氏内阿米巴、结肠内阿米巴、夏氏内阿米巴和波列基内阿米巴的18S核糖体RNA基因,调查内阿米巴流行情况。对从藏酋猴粪便中分离的诺氏内阿米巴进行培养,抽提基因组DNA后进行PCR,扩增多丝氨酸内阿米巴蛋白(SRP)和t RNA间隔区的短重复序列(t RNA-STR),进行克隆测序和多态性分析。同时扩增我国广西龙虎山、贵州黔灵山、四川峨眉山和安徽黄山的野生猕猴的线粒体HVR-I基因和HVS基因,进行克隆测序和多态性分析。结果 PCR结果显示,60份粪便样品中诺氏内阿米巴、结肠内阿米巴和夏氏内阿米巴的阳性率分别为58.3%、25%和86.7%,而溶组织内阿米巴、迪斯帕内阿米巴和波列基内阿米巴均为阴性。成功从藏酋猴粪便中分离培养了6株诺氏内阿米巴并进行遗传多态性分析。基于SRP基因和t RNA-STR基因进行群体遗传结构分析显示,中国不同地区诺氏内阿米巴种群各具特征,其中贵阳恒河猴分离株的遗传多样性明显。Mental检验结果显示使用t RNA-STR基因计算的中国诺氏内阿米巴各地理种群间的遗传距离与地理距离存在显著的相关性(R2=0.809),表明中国诺氏内阿米巴基于t RNA-STR基因的遗传分化关系满足距离隔离模式。而多因素回归分析发现,使用t RNA-STR基因计算的中国诺氏内阿米巴各地理种群间的遗传距离与地理距离、使用HVR-I和HVS基因计算的野生猕猴各地理种群间的遗传距离有更显著的相关性(R2=0.852),表明宿主遗传多样性也是诺氏内阿米巴遗传多样性的影响因素。结论本研究显示中国黄山野生藏酋猴有较高的诺氏内阿米巴感染率,目前本实验室调查的所有中国野生猕猴种群均有诺氏内阿米巴的流行。通过对比分析诺氏内阿米巴各地理种群具分类意义的基因型,发现中国不同地区诺氏内阿米巴种群遗传多态性各具特征,其影响因素可能包括地理和宿主遗传多样性因素,为进一步对诺氏内阿米巴虫株溯源提供理论依据。

关键词：诺氏内阿米巴恒河猴藏酋猴遗传多样性

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Genome Sequencing of Duddingtonia flagrans and Transcriptome Analyses of Nematode-trapping Related Genes in Duddingtonia flagrans

Genome Sequencing of Duddingtonia flagrans and Transcriptome...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Rui Wang Wei Zhang Meng Liang Xinfang Wang Xiaorui Zhu Wenrui Wang Xiaoping Luo Lianru Yang Xiaoye Yang College of Veterinary Medicine Inner Mongolia Agricultural University Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences

Duddingtonia flagrans is one of the most well-studied and widely utilised nematode-trapping fungi,as it has high environmental tolerance and can pass through the gastro-intestinal tract of grazing livestock without reducing its germination rate and the efficiency of ***,*** has been widely used as a biological agent in prevention and control of animal parasitic *** far,*** has been studied on ultrastructure observation,biology research to evaluation of clinical *** *** has been widely used as a promising biocontrol agent,little is known how it possessed the ability to control nematodes and its trophic modes at the molecular *** order to investigate the features and advantages of *** in control of nematodes,the draft genome and transcriptome of *** were sequenced and studied by comparative *** genome and transcriptome analysis provided a series of differently expressed genes related to nematode-trapping and some of the genes were validated by Real-time quantitative ***,the interaction between *** and infective larvae of Strongylus equinus was checked with optical microscope as well as scanning electron *** order to provide the research material for the study of gene function using gene knockout technology,the protoplast-producing efficiency of *** was evaluated by fluorescence *** main research achievements were as follows:(1) The method of genome DNA extraction of *** was explored,and the result revealed that the DNA extraction method of solid culture systems with dialysis membrane combined with spin columns was simple and *** genome survey was carried out to determine the research strategy which was fit for the *** result showed the average genome size of *** was about 37.6 Mb and GC content was as high as 44.95% with low hybridity *** genome survey has laid a theory foundation for the detailed genome

关键词： Nematode-trapping fungus Duddingtonia flagrans Genome Transcriptome Fluorescence labeling of protoplast

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Prevalence of Cryptosporidium,Microsporidium and Isospora infection in HIV-infected people: a global systematic review and meta-analysis

Prevalence of Cryptosporidium,Microsporidium and Isospora in...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ze-Dong Wang 刘全 Huan-Huan Liu Shuang Li Li Zhang Yong-Kun Zhao 朱兴全 State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Military Veterinary Institute Academy of Military Medical SciencesKey Laboratory of Jilin Province for Zoonosis Prevention and Control Central Laboratory Inner Mongolia General Forestry Hospital College of Life Science Jilin Agricultural University

Background: Diarrhea caused by opportunistic intestinal protozoa is a common problem in HIV *** aimed to establish the prevalence of Cryptosporidium,Microsporidia,and Isospora in HIV-infected people,which is central to develop public policy and clinical ***: In this systematic review and meta-analysis,we searched Pub Med,Embase,Google scholar,Science Direct,Chinese Web of Knowledge,Wanfang,and Chongqing VIP databases for studies reporting Cryptosporidium,Microsporidium,or Isospora infection in HIV-infected *** extracted the numbers of people with HIV and protozoa infection,and estimated the pooled prevalence of parasite infection by a random effects ***: Our research identified 130 studies that reported Cryptosporidium,Microsporidium,and Isospora infection in HIV-infected *** estimated the pooled prevalence to be 14.0%(95% CI 13.0–15.0,3,283/43,218) for Cryptosporidium,12.4%(10.5–14.2,985/15,334) for Microsporidium,and 2.5%(2.1–2.9,788/105,922) for *** prevalence of Microsporidium and Isospora infection was found in high-income countries,and high prevalence of Cryptosporidium and Isospora infection was found in sub-Saharan *** also detected high prevalence of Cryptosporidium,Microsporidium,and Isospora infection in diarrhea *** analysis showed that three studies significantly affect prevalence of Isospora,which was adjusted to 5.0%(4.1–5.9,469/8,570) by excluding these ***: Our findings suggest that HIV-infected people have a high prevalence of Cryptosporidium,Microsporidium,and Isospora infection in low-income countries and diarrhea people,especially in sub-Saharan Africa,reinforcing the importance of routine surveillance for opportunistic intestinal protozoa in HIV-infected people.

关键词： HIV Cryptosporidium Microsporidium Isospora Meta-analysis

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Evidence of Triodontophorus species belong to Cyathostominae from the mtDNA sequences

Evidence of Triodontophorus species belong to Cyathostominae...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yuan Gao Jun-Feng Gao Hong Duan Wen-Wen Xu Yan Zhang Chun-Ren Wang College of Animal Science and Veterinary Medicine Heilongjiang Bayi Agricultural University College of Life Science and Biotechnology Heilongjiang Bayi Agricultural University

Equine strongyles are classified into Strongylinae(large strongyles) and Cyathostominae(small strongyles) based on the morphological *** the significant large intestinal nematode pathogens of horses,the classification of these parasitic nematodes is ***(mt) genomes are used to address classification ***,the objectives of this study were to determine four Cyathostominae nematode species(Cylicocyclus nassatus,Cyathostomum catinatum,Cylicostephanus minutus,and Poteriostomum imparidentatum) and four Strongylinae nematode species(Triodontophorus brevicauda,Triodontophorus nipponicus,Triodontophorus serratus,Strongylus equinus) complete mt genomes,compared with all the Strongylidae nematodes and reconstruct the phylogenetic relationships among Strongyloidea to test the hypothesis that Triodontophorus *** to *** mt genomes of ***(13,846 bp),***(13,838 bp),***(13,826 bp),***(13,817 bp),***(14,305 bp),***(13,701 bp),and ***(13,794 bp) ***(14,545 bp) contained 12 protein-coding genes(nad1–nad6 and nad4 L,cox1–cox3,cytb,atp6),22 t RNA genes,two r RNA genes,and were all transcribed in the same *** analysis showed that Triodontophorus species had relatively high identity with Cyathostominae nematodes,rather than Strongylus(*** and ***) species of the same subfamily(Strongylinae).Compared the mt DNA gene order of Strongyloidea nematodes sequenced to date revealed that all the nematodes in this superfamily possess identical gene *** analyses employed mt DNA data showed that three Triodontophorus species were closer to Cyathostominae species instead of Strongylus *** present study first determined the complete mt genome sequences of eight Strongylidae nematodes,which provide novel genetic markers for further studies of Strongylidae taxonomy,population genetics,and ***,sequen

关键词： Complete mitochondrial genome Strongylidae Cyathostominae Strongylinae Comparative Analysis Phylogenetic Analysis

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Deltamethrin exposure induced alteration of amino acids level in the wild deltamethrin-resistant strain of Aedes albopictus

Deltamethrin exposure induced alteration of amino acids leve...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ruili Xie Lianfen Huang Lilan Peng Xiaoguang Chen Hua Li Department of Pathogen Biology & Experimental teaching center of preventive medicine School of Public HealthSouthern Medical University

Objective Observe the level alteration of amino acids in aedes albopictus larvae undergoing deltamethrin exposure in oder to explore the biomarkers related to deltamethrin toxic ***: The Aedes albopictus larvae were collected from the Yuexiu district of Guangzhou city where was reported that the mosquitoesare highly resistant to *** F1 generation larvae were determined deltamethrin resistance according to WHO recommended detection method and were treated with 0.035 ppm deltamethrin(dissolved in acetone) and acetone respectively(10 larvae in each group).The amino acids level in the larvae before and after deltamethrinand acetone exposure were detected by LC-MS/MS,and 6 times repeated detection were completed for each ***: The LC50 of the F1 generation larvae of the wild strain aedes albopictus was 0.064806 ppm,which reached 10.249 times of that of the sensitive strain(LC50=0.006323 ppm).It can be identified as resistant strain according to WHO criteria(more than 5 times).There were 11 of the 17 common amino acids showed differences in level post drug exposure(p<0.05) in the larvae by LC-MS/MS test,in which,the level of 10 amino acids(alanine,citrulline,glutamate,glycine,leucine,ornithine,phenylalanine,proline,serine,threonine) reduced and the level of histidineelevated after exposure(p<0.05).The level alteration of 7 amino acids( glutamate,glycine,ornithine,proline,serine,threonine and histidine) were related to acetone exposure(p<0.05) and the level reduction of 4 amino acids(alanine,citrulline,leucine and phenylalanine) were attributed to deltamethrin exposure(p<0.05) eliminated the effect of ***: Both deltamethrin and acetone may act the effect factors of the level alteration of amino acids in the Aedes albopictus larvae,and deltamethrin exposure could result in the level alteration of alanine,leucine,phenylalanine and citrulline in the *** 4 amino acids might become thecandidate biomarkers for deltamethri

关键词： deltamethrin Aedes albopictus resistance amino acids

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A recombinant multi-epitope vaccine induced T follicular helper cells for the enhancement of humoral immunity against Trichinella spiralis

A recombinant multi-epitope vaccine induced T follicular hel...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yuan Gu Bo Li Ximeng Sun Xinping Zhu Department of Medical Microbiology and Parasitology School of Basic Medical SciencesCapital Medical University

Background: As one of the most popular parasitic zoonoses,Trichinellosis is eliminated through humoral immunity in a great deal.T follicular helper cells（Tfh） are a newly identified CD4 T cells subset which plays an important role in providing of cognate B cell *** cells are critical for humoral immunity,including the generation of long-lived and high affinity plasma cells and memory cells which are crucial for long-term protection against *** previous studies have identified that mice immunized with a recombinant multi-epitope vaccine（r MEP） with Th2 epitope and B cell epitope in series produced long-term antibody responses and protective immunity against Trichinella spiralis *** is speculated that Tfh cells may play an important role in supporting B cell responses and the aim of this study is to explore the mechanism of Tfh in humoral responses induced by r ***: Female BALB/c mice were subcutaneously immunized with r MEP emulsified with ISA 50 V2 adjuvant.r MEP-specific IgG,IgG1 and IgG2 a antibody responses were assessed by *** cells of spleen and inguinal lymph nodes,GC B cells and memory B cells of splenocytes were analysed by flow ***: r MEP induced high levels of anti-r MEP specific IgG and subclass IgG1 and IgG2 a,with IgG1 *** to the control,Tfh cells of spleen and inguinal lymph nodes,GC B cells and memory B cells of splenocytes,as well as the IL-4,IFN-γ and IL-21 Tfh cells,increased in r MEP immunized group,as analysed by flow ***: r MEP induced a mixed Th1/Th2 immune response in mice,with Th2 *** cells,GC B cells as well as memory B cells induced by r MEP played an important role in generating long-lasting and effective humoral immune responses.

关键词： Trichinella spiralis humoral immunity T follicular helper cells multi-epitope vaccine

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