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检索条件"任意字段=中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会"

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miRNA profiling of Protoscoleces in Echinococcus granulosus

miRNA profiling of Protoscoleces in Echinococcus granulosus

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： WANG Zheng-rong BO Xin-Wen ZHANG Yan-Yan Ma Xun Wang Zhi-Xin Lu Ping-Ping Meng Ji-meng State Key Labaratory of Sheep Genetic Improvement and Healthy Production/Institute of Animal Husbandry and Veterinary Xinjiang Academy of Agricultural and Reclamation Sciences College of Animal Science and Technology Shehezi University College of Veterinary Medicine China Agricultural University

A peculiar feature of the biology of *** is that the protoscolex has the potential to develop in either of two *** ingested by a dog will develop in a sexual direction to form adult tapeworms in the definitive host *** contrast,if a hydatid cyst ruptures within the intermediate or human host,each released PSC is capable of differentiating asexually into a new hydatid *** unique developmental phenomenon has been well known,but up to date,the regulation mechanism behind the phenomenon is little *** RNAs are endogenous small non encoding RNA molecules,which plays a pivitol role in the animal's post transcriptional regulation *** order to explore the role of mi RNA in the PSC development process,the present study analyzed the mi RNA profiling of *** PSC by RNA *** results showed that there are 109 known mi RNAs and 189 novel mi RNA hairpin precursors detected in the PSC of the *** target genes prediction of these mi RNAs suggested that they are widely involved in the growth of the PSC related Key signaling pathways,such as Wnt,c AMP,Hedgehog,NF-κB,Notch,and bile acids signaling *** further research,the expression and distribution of mi RNAs and thier canidated target genes,which are association with the key genes of classic wnt signaling ***,our findings suggested that there maybe a potential regulatory relationship between HG328413.16258 vs β-catenin,HG328414.16364* vs Dsh and HG328399.15846 vs *** summary,the results of the present study suggest that mi RNAs may participate in a wide range of developmental process in PSC,which needs a further verified.

关键词： miRNA Echinococcus granulosus protoscoleces expression profile target gene

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Seroprevalence of Toxoplasma gondii infection in arthritis patients in eastern China

Seroprevalence of Toxoplasma gondii infection in arthritis p...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ai-Ling Tian Yuan-Lin Gu Na Zhou Wei Cong Guang-Xing Li Hany M.Elsheikha Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Weihaiwei People’s Hospital Affilliated Hospital of Medical College Qingdao University School of Basic Medicine Dali University Faculty of Medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus

Background: There is accumulating evidence for an increased susceptibility to infection in patients with *** sought to understand the epidemiology of Toxoplasma gondii infection in arthritis patients in eastern China,given the paucity of data on the magnitude of *** infection in these ***: Seroprevalence of *** infection in 820 arthritic patients and an equal number of healthy controls,from Qingdao and Weihai cities,eastern China,was assessed by enzyme-linked immunosorbent assay using a crude antigen of the *** on sociodemographic,clinical and lifestyle characteristics of the study participants was also ***: The prevalence of *** Ig G was significantly higher in arthritic patients(18.8%) compared with 12% in healthy controls(P < 0.001).12 patients with arthritis had *** Ig M antibodies comparable with 10 of the controls(1.5% vs 1.2%).Demographic characteristics did not significantly influence these seroprevalence *** highest seropositivity rate of *** infection was detected in patients with rheumatoid arthritis(24.8%),followed by reactive arthritis(23.8%),osteoarthritis(19%),infectious arthritis(18.4%) and gouty arthritis(14.8%).Seroprevalence rates of rheumatoid arthritis and reactive arthritis were significantly higher when compared with controls(P < 0.00 and P < 0.05,respectively).A significant association was detected between the presence of cats in the home and *** infection in arthritic patients(odds ratio [OR],1.68;95% confidence interval [CI],1.24 to 2.28;P = 0.001).Conclusions: These findings are consistent with and extend previous results by providing more evidence to support the link between contact with cats and an increased risk of *** *** study also confirmed an association between *** infection and arthritis patients in China for the first *** for better prevention and control of *** infection in arthritis pati

关键词： Toxoplasma gondii Autoimmunity Arthritis Seroprevalence Risk factors

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Protective efficacy of recombinant Cryptosporidium parvum CpPRP1 sushi domain against *** infection in mice

Protective efficacy of recombinant Cryptosporidium parvum Cp...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yan HUANG Wei CAO Kai SHI Rongsheng MI Xiangan HAN Zhaoguo CHEN Key Laboratory of Animal Parasitology of Ministry of Agriculture Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards (Shanghai) of Ministry of AgricultureShanghai Veterinary Research InstituteChinese Academy of Agricultural Sciences

Until now there are no completely effective parasite-specific pharmaceuticals or immunotherapies for treatment against the zoonotic *** one Cryptosporidium parvum rhoptry protein candidate related to Tg RON1 of *** and Pf ASP of *** has been *** domain(Cp Sushi) is an important functional domain in Cryptosporidium parvum putative rhoptry protein-1(Cp PRP1) which is the only reported *** rhoptry protein and may play key role in the course of ***,subcellular fractionation of *** sporozoites was performed to obtain highly purified ***-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis followed by liquid chromatography coupled with mass spectrometry was applied for fraction analysis.A 708 bp fragment encoding the Cp Sushi domain was amplified and expressed in *** detection showed that Cp Sushi was located on the surface of *** oocysts and the apical pole to the sporozoites that belonged to the position of ***-week-old female ICR mice were used for detecting the immunoreactions and immunoprotection of recombinant Cp Sushi(r Cp Sushi) to artificial *** *** results indicated that 22 potential novel rhoptry proteins were detected by protein domain analysis using online softwares.A significant increase of anti-Cp Sushi antibody response was induced by the recombinant *** to blank,Tris-EDTA(TE) buffer and adjuvant controls mice,r Cp Sushi-immunized mice produced specific in vitro spleen cell proliferation as well as enhanced IL4,IL5,IL12 p70 and TNF-α *** reduction rate of parasites shedding in stool in mice immunized with r Cp Sushi was 68.91% after challenging with *** results suggested that Cp Sushi could be a new promising cryptosporidiosis vaccine candidate antigen composition.

关键词： Cryptosporidium parvum putative rhoptry protein-1 sushi domain localization protective immunity

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CRISPR/Cas9-based construction of AaaH1/2 deficient strain of Toxoplasma gondii and its functional study

CRISPR/Cas9-based construction of AaaH1/2 deficient strain o...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yushu Qiu Hongmei Chen Min Liu Xiaoguang Chen Kun Wu Guangdong Provincial Key Laboratory of Tropical Disease Research Department of Pathogen BiologySchool of Public HealthSouthern Medical University

Toxoplasma gondii is a highly neurotropic parasite in *** infection rates are estimated at 5–30% globally.A growing number of evidence indicates that latent *** infection in the central nervous system（CNS） may be responsible for behavioral changes even in immunocompetent mammalian hosts,including *** experiments in vitro and in vivo showed that *** infection could affect the dopamine level of the *** phenomenon suggests that dopamine pathway may be one of the mechanisms for behavioral changes of *** hydroxylase catalyzes the conversion of tyrosine to levodopa（L-DOPA） is the rate limiting step in the synthesis of *** contains two genes encoding tyrosine hydroxylase: Aaa H1 and Aaa *** recent years,bioinformatics analysis revealed that Tg Aaa H contains a precursor of signal peptide and could be secreted outside the parasite to *** Aaa H1 was expressed in tachyzoites and bradyzoites,while Tg Aaa H2 was only expressed in the latter ***,it is significant to analyze the function of Tg Aaa H at different *** our study,we cloned and sequenced Tg Aaa H of RH and Pru strain,and identified the copy number of Tg Aaa H in RH,ME49 and Pru strains *** Aaa H1/2 deficient strain was constructed based on CRSPR/Cas9 system.E-CRISP database was used to design g RNA;mutation of g RNA in p SAGl::Cas9-U6::sg UPRT plasmid was performed by using site-directed mutagenesis to construct p SAGl::Cas9-U6::sg Aaa H *** p UCl9 donor DNA plasmid was constructed by infusion enzyme and fragments were amplified by *** plasmid p SAGl:Cas9-U6:sg Aaa H and donor DNA fragments were electroporated into RH and Pru strain *** electroporation,suspension was inoculated into human foreskin fibroblast cells（HFF）.The 3μ mol/L pyrimethamine was used to screen the electroporated parasite and monoclone was detected by *** research of the function of Tg Aaa H1/2 is still in progress.

关键词： Toxoplasma gondii Tryrosine hydroxylase CRISPR/Cas9 system

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Studies on the Biological Characteristics and Fermentation

Studies on the Biological Characteristics and Fermentation

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Xiaorui Zhu Meng Liang Xinfang Wang Wenrui Wang Xiaoping Luo Lianru Yang Xiaoye Yang Rui Wang College of Veterinary Medicine Inner Mongolia Agricultural University Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences

Pochonia chlamydosporia is an important biocontrol *** fungus can be used to control not only plant parasitic nematodes but also animal parasitic *** has great potential to control parasitic *** order to obtain more information about *** *** morphological characteristics,fermentation broth and it’s effect on animal parasitic nematodes were *** results are listed as follows: The colony morphology growth rate of the fungus grew on the 0.4 g/L corn-meal-agar medium was slightly different with that grew on Savary-gilliard glucose agar *** colony radius was 2.4 cm on CMA and 3.0 cm on SDA,*** hyphae on the two medium were both radial,thick,and *** were single or verticillate born in *** conidiophore had 4 branches,and there was an oval or round transparent conidium on the top of each *** with thick wall was formed in the aged hyphae and each was brick lattice shape and *** Caenorhabditis elegans and eggs,Strongylus larvae and eggs,Ascaris suum eggs were put on the CMA medium surface with the *** results showed that the infection rate of the fungus to *** eggs and Strongylus eggs was only 2%;The fungus can break the eggshell and infect the eggs,and the infection rate was 29.6% after one *** different liquid medium were used to obtain fermentation broth of *** *** fermentation broth filtrations were collected,and the protein content,enzyme activity,phosphatase activity and so on were *** results showed that the indicators of the fermentation broth were also different in different *** protein content and enzyme activity of LMZ medium,which also had the highest inhibition rate to the tested nematodes,was the highest among the four and the corn was the lowest *** the study,the morphological characteristics,the effect on animal parasitic nematodes and growth in four different li

关键词： Pochonia chlamydosporia Conidiophore Chlamydospore Liquid Fermentation

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Serum levels of cytokines in water buffaloes experimentally infected with Fasciola gigantica

Serum levels of cytokines in water buffaloes experimentally ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Fu-Kai Zhang Ai-Jiang Guo Jun-Ling Hou Miao-Miao Sun Zhao-An Sheng Xiao-Xuan Zhang Wei-Yi Huang Hany M.Elsheikha Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences College of Animal Science and Technology Guangxi University Faculty of Medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Fasciola gigantica infection in water buffaloes causes significant economic losses especially in developing *** modulation of the host immune response by cytokine neutralization or vaccination is a promising approach to control infection with this parasite,our understanding of cytokine's dynamic during *** infection is *** address this,we quantified the levels of serum cytokines produced in water buffaloes following experimental infection with *** buffaloes were infected via oral gavage with 500 viable *** metacercariae and blood samples were collected from buffaloes one week before infection and for 13 consecutive weeks *** levels of 10 cytokines in serum samples were simultaneously determined using *** failed to elicit the production of various pro-inflammatory cytokines,including interleukin-1β(IL-1β),IL-2,IL-6,IL-12,and IFN-γ.On the other hand,evidence of a Th2 type response was detected,but only early in the course of parasite colonization and included modest increase in the levels of IL-10 and *** results also revealed suppression of the immune responses as a feature of chronic *** infection in *** together,*** seems to elicit a modest Th2 response at early stage of infection in order to downregulate harmful Th1-and Th17-type inflammatory responses in experimentally infected *** full extent of *** immune response and its relation to pathogenesis requires further study.

关键词： Buffaloes Cytokines Fasciolosis Th1/Th2 paradigm

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Metabolomic profiling of Eimeria tenella life cycle stages using liquid chromatography-mass spectrometry

Metabolomic profiling of Eimeria tenella life cycle stages u...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Shenquan Liao Nanshan Qi Caiyan Wu Juan Li Minna Lv Xuhui Lin Linzeng Yu Jianfei Zhang Mingfei Sun Institute of Animal Health Guangdong Academy of Agricultural SciencesOpen Laboratory of Veterinary Public Health and Key Laboratory of Livestock Disease Prevention of Guangdong Province

Purpose: The protozoan parasite Eimeria tenella,is considered one of the most pathogenic species and causes great economic loss to the poultry *** has a complex developmental life cycle including an exogenous phase in the environment and an endogenous phase in the *** the life cycle parasites need to adapt to different circumstances and undergo metabolism ***,little is known about the metabolic pathways in ***,especially the metabolite changes during the invasive *** conducted untargeted metabolomic analysis of four life cycle stages of ***(unsporulated oocyst,sporulated oocyst,sporozoite,and second-generation merozoite).Methods: Unsporulated oocysts of ***(Guangdong strain) were harvested from *** oocysts were sporulated in vitro for 48 *** were obtained from cleaned oocysts by in vitro excystation and purified over ***-generation merozoites were recovered from the caecal mucosa of chickens 120 h after *** purified samples were collected by centrifugation and frozen at-80 °C *** methanolic extracts were prepared and analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Results: The metabolomics analysis detected approximately 933 metabolite *** partial least squares discriminant analysis(PLS-DA),a total of 20,56 and 74 differential metabolites were identified in the positive ion(ESI+) mode,34,33 and 46 differential metabolites were detected in the negative ion(ESI-) mode in sporulated oocyst,sporozoite and second-generation merozoite compared to unsporulated oocyst,*** these metabolites,41 were shared between sporozoite and second-generation merozoite compared to unsporulated oocyst in the ESI+ mode,and 17 were shared in the *** 58 differential metabolites were involved in carbohydrate metabolism,lipid metabolism,amino acid metabolism,cofactor and vitamin *** results showed the metabolic chan

关键词： Eimeria tenella metabolomic profiling liquid chromatography mass spectrometry

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Diversity and Function of Bacterial Microbiota in Aedes albopictus

Diversity and Function of Bacterial Microbiota in Aedes albo...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Xiaoming Wang Kexing Huang Yijia Guo Kun Wu Guiyun Yan Xiao-Guang Chen Key Laboratory of Prevention and Control for Emerging Infectious Diseases of Guangdong Higher Institutes Department of Pathogen BiologySchool of Public HealthSouthern Medical University Program in Public Health School of MedicineUniversity of CaliforniaIrvineUSA

Background: Aedes albopictus(Diptera: Culicidae) is the most important vector of arboviruses such as dengue and Zika ***'s also a highly invasive and very aggressive *** population control is currently the only effective method for dengue *** microbiota is ubiquitous and is intimately associated with invertebrates and vertebrates in ***,it's still unknown about the effects of microbiota on mosquito development and ***: Life table studies were conducted to determine the impact of bacterial microbiota in larval habitats on Aedes albopictus mosquito larval survivorship and *** Seq pyrosequencing of 16 S r RNA gene V4 hyper-variable region was used to examine the diversity and dynamics of microbiota in aquatic habitats,larvae and adult mosquitoes under the laboratory and natural *** assemblages of bacterial microbiota in Aedes albopictus wildtype strain,Wolbachia-free strain and a strain transfected by new types of Wolbachia were also examined by High-Throughput ***: Life table analysis found 6% larval-to-pupal survival rate in water from natural larval habitats,bacterial removed by filtration led to 3% pupation rate,but bacterial depletion of the same water using ampicillin antibiotics resulted in 0% pupation *** bacterial microbiota diversity was found in aquatic habitats,and each type of aquatic habitats possessed a large number of unique *** of adult mosquitoes differed significantly from those in the habitat *** diversity generally decreased from aquatic stage to *** content of Wolbachia increased with the age of mosquitoes in both laboratory strain and wild strain.q PCR revealed that w Alb A and w Alb B strains were the major components,and w Alb B was the dominant *** of Wolbachia from Aedes albopictus from the use of antibiotics and transfection of exogenous Wolbachia strain reshaped the composition of bacterial

关键词： Aedes albopictus Bacterial microbiota Diversity Function Wolbachia

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Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis

Profiling of the perturbed metabolomic state of mouse spleen...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Xiao-Qing Chen Chun-Xue Zhou Hany M.Elsheikha Shuai He Gui-Xue Hu Xing-Quan Zhu Department of Veterinary Medicine College of Animal Science and TechnologyJilin Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Department of Parasitology Shandong University School of Basic Medicine Faculty of Medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Animal Science and Technology Anhui Agricultural University

Toxoplasma gondii,a common opportunistic protozoan,is a leading cause of illness and mortality among immunosuppressed individuals and during congenital *** therapeutic strategies for toxoplasmosis are not fully effective at curtailing disease progression in these *** the parasite's ability to influence host immunity and metabolism,understanding of the metabolic alterations in the host's immune organs during *** infection may enhance understanding of the molecular mechanisms that define the pathophysiology of *** *** investigated the global metabolic changes in the spleen of BALB/c mice at early and late stage of infection with *** using LC-MS/MS-based *** data analysis methods,principal components analysis(PCA) and partial least squares discriminant analysis(PLS-DA),were used to identify metabolites that are influenced by *** *** analyses clearly separated the metabolites of spleen of infected and control mice.A total of 132 differential metabolites were identified,23 metabolites from acutely infected versus control mice and 109 metabolites from chronically infected versus control ***,hormones,lactones,acids,peptides,antibiotics,alkaloids and natural toxins were the most influenced chemical *** were 12 shared differential metabolites between acutely infected versus control mice and chronically infected versus control mice,of which 4,4-Dimethyl-5 alpha-cholesta-8,14,24-trien-3 beta-ol was significantly upregulated and Ubiquinone-8 was significantly *** perturbed metabolic pathways included primary bile acid biosynthesis,steroid hormone biosynthesis,biotin metabolism,and steroid biosynthesis,with arachidonic acid metabolism being the most significantly impacted *** metabolic changes suggest a multifactorial nature of the immunometabolic responses of mouse spleen to *** *** study demonstrated that *** infection can

关键词： Toxoplasma gondii Spleen Mass spectrometry Metabolome Non-targeted metabolomics Pathway enrichment analysis

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First genetic characterization of Toxoplasma gondii infection in poultry meat intended for human consumption in Eastern China

First genetic characterization of Toxoplasma gondii infectio...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Lan-Bi Nie Yang Zou Nian-Zhang Zhang Xiao-Jie Xu Feng-Cai Zou Xing-Quan Zhu Wei Cong State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences College of Animal Science and Technology Jilin Agricultural University College of Marine Science Shandong University at Weihai College of Animal Science and Technology Yunnan Agricultural University

Toxoplasma gondii can infect nearly all warm-blooded animals including ***,limited information on the molecular epidemiology and genotypes of *** infecting poultry is available in ***,the present study characterized *** genotypes in poultry meat in eastern *** August 2015 and September 2016,muscle tissue samples collecting from 414 poultries(257 chickens,115 ducks and 42 geese) in Shandong provinces were used to detect the *** B1 gene by a semi-nested PCR,and the positive samples were genotyped at 10 nuclear loci(i.e.,SAG1,alternative SAG2,5'-and 3'-SAG2,SAG3,L358,BTUB,c22-8,GRA6,c29-2,PK1) and an apicoplast locus Apico by multi-locus PCR-RFLP ***-two(7.37%) samples from 414 poultry meat were *** B1 gene *** had the highest *** prevalence(8.17%),followed by ducks(7.83%) and geese(4.76%).Furthermore,only one genotype(Toxo DB#9) was *** is the first genetic characterization of *** isolates from poultry meat in Shandong province,eastern China and also the first report of genotype Toxo DB#9 was found in poultry in China,which provide basic data for the surveillance and control of *** infection in poultry,other animals and humans.

关键词： Toxoplasma gondii Poultry meat Genetic characterization China

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