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检索条件"任意字段=中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会"

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Screening of small molecular inhibitors targeting at Toxoplasma gondii autophagy-related protein TgAtg8-TgAtg3 interaction

Screening of small molecular inhibitors targeting at Toxopla...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Shu Xian Liu Yan Wang Xin Hu Feng Tan Department of Parasitology in Wenzhou Medical University Institute of Tropical Medicine in Wenzhou Medical University

Objective: To acquire small molecule inhibitors that could significantly reduce the proliferation of Toxoplasma gondii and have no(or low) toxicity to human ***: Primarily,Alphascreen and SPR assays were used to detect the inhibitory activity of small molecule compounds on human LC-Atg3 、 rat Atg8-Atg3 protein-protein interaction,select compounds that had no effect on human Atg8-Atg3 interaction,thus obtain the small molecule compounds which could specifically impact on Toxoplasma gondii and have no influence in Atg8-Atg3 interaction of human ***,we evaluated the specific inhibition effects of small molecule inhibitors at the level of cytology experiments: we used CCK-8 assays and plaque assays to choose the small molecule compounds that have no toxicity to host cells but inhibit the growth of Toxoplasma gondii,then further groped the concentration of the chosen inhibitors to identity their IC50 to Toxoplasma gondii through intracellular replication *** estimate the internal effect of Toxoplasma gondii infected mice model by ***: Experiments of Alphascreen and SPR had already screened 12 small molecule inhibitors and a small molecule agonist,these 13 small molecule compounds are clinical *** assays and intracellular replication assays indicated that 7 small molecular compounds in our lab have definitely inhibitory activity on the proliferation of Toxoplasma gondii and D4,D6 in 7 small molecular compounds could signifcantly reduce the proliferation of Toxoplasma gondii whose IC50 to Toxoplasma gondii is around 4.5 u M and 1.2 u M,***,CCK-8 assays indicate that the IC50 of D4,D6 to Toxoplasma gondii have no toxicity to human ***: It was illuminated preliminary that D4 and D6 could reduce the proliferation of Toxoplasma gondii

关键词： Toxoplasma gondii protein-protein interaction Atg8 Atg3 small-molecule inhibitor

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Influence of Mosquito Age on the Susceptibility of Anophele stephensi to Plasmodium Infection

Influence of Mosquito Age on the Susceptibility of Anophele ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： SONG Xiu-mei WANG Jing-wen School of Life Sciences Fudan University

Objective To study the influence of Anophelesstephensi age on mosquito's ability to transmit Plasmodiumberighi and to elucidate the possible *** To study and compare the susceptibility of *** of different ages to Plasmodium,4-day and 25-day female *** were feed with BALB/c mice blood which was infected with 4%-8% Plasmodium *** 24 h after blood feeding,the mosquitoes that were not fully fed with blood were abandoned.8 days after infection,mosquitoes were dissected and microscopy was used to check the oocysts numbers in mosquito midgut,and the oocysts numbers in the 4-day and 25-day mosquitos were *** study bacteria load in these two group mosquitos,LB plate was used to detect the cultural bacteria and q PCR was performed to check the total bacteria load in the two *** expression levels of major immune response effectors including cecropin(CEC1,CEC3),defensine(DEF),gambicin(GAM),attinsin(ATT),nitric oxide synthase(NOS),dual oxidase(DUOX),and thioester protein 1(TEP1) in 4-day and 25-day mosquitos were compared by q *** At 8-day after infected,the median oocysts number in 4-day mosquitos was 139,which was nearly 46 times of that in 25-day mosquitos(median,3)(P<0.01).There was also a significant difference in the intestinal bacteria load between 4-day and 25-day *** of the q PCR show that the total bacteria load in 25-day mosquitos was 1.5 times of that in 4-day mosquito(P<0.05).By LB plate culture,proliferation of 28 889 cfu bacteria was found in 25-day mosquitos,which was 9 times of that in 4-day mosquitos(3 200 cfu)(P<0.05).In addition,the immune response effectors between 4-day and 25-day mosquitos were significantly *** NOS expression level in 25-day was 2.4 times of that in 4-day mosquitos(P<0.01),while the expression levels of antimicrobiota peptide ATT,DEF,CEC3,and CEC1 in 4-day mosquitos were only 27%,48%,14%,61% of those in 25-day mosquitos,respectively(P<0.05).Conclusion T

关键词： age immune response microbiota Plasmodium berghei

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Elucidation of Transformer-2in Aedes albopictus and its effect on ovarian development

Elucidation of Transformer-2in Aedes albopictus and its effe...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Xiaocong Li Binbin Jin Yunqiao Dong Zhijian Tu Xiaoguang Chen Jinbao Gu Guangdong Provincial Key Laboratory of Tropical Disease Research Department of Pathogen BiologySchool of Public HealthSouthern Medical University Reproductive Medical Centre of Guangdong Women and Children Hospital Department of Biochemistry Virginia Tech

In Drosophila melanogaster,transformer-2(tra-2) gene plays an essential regulatory role in sex-specific splicing of conserved master-switch gene doublesex(dsx) and courtship master regulator gene fruitless(fru)in sex determination cascade,and triggers somaticand germline sexual differentiation and,also regulates sexual *** the other hand,the molecular characteristicsand function of mosquitotra-2 remains to be further *** the present study,sequences and expression profiles oftra-2 s in the Asia tiger mosquito,Aedes albopictus were ***-2 paralogues,designated as Aalbtra2-α,Aalbtra2-β and Aalbtra2-γwere *** analysis revealsfirst duplication event occured in common ancestor of Culicidae,and generated Aalbtra2-β,thesecond duplication is estimated to have occurred after the divergence of *** and ***,that give a rise of Aalbtra2-γ.Besides of the conserved RNA-recognition motif(RRM),arginine-rich/serine-rich regions(RS-domains)and a linker region were identified,a glycine-rich region,located between RRM and RS2 was observedin Aalbtra2-α and Aalbtra2-γ,which first reported in Diptera insecttra-2 *** real-time PCR showed that all threetra-2 paralogueswere relatively highly expressed in the fertilized embryo,suggesting their maternal origin in early embryonic developmental ***,all Aalbtra-2 s were significantly highly expressed in the ovary,while Aalbtra-2 s was highly expressed in male *** explore whether Aalbtra-2 s involved in regulation sex-specific alternative splicing of dsx and ***-mediated knockdown of Aalbtra-2 s revealed that all Aalbtra-2 s showed no effect both on the variation in the splicing pattern of dsxand frupre-m RNA and the relative expression levelof dsxand *** downregulation of Aalbtra2-γ and Aalbtra2-α in female adult can still causedecrease in ovariole number and average follicle size post blood meal bypass the dsx,these results indicate that

关键词： Sex determination Aedes albopictus transformer-2 RNAi-mediated knockdown doublesex fruitless

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Evidence of increased multiple-insecticide resistance in urban Aedes albopictus population

Evidence of increased multiple-insecticide resistance in urb...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yiji Li Jiabao Xu Daibin Zhong Hong Zhang Wenqiang Yang Guofa Zhou Xinghua Su Yang Wu Kun Wu Songwu Cai Guiyun Yan Xiao-Guang Chen Department of Pathogen Biology Guangdong Provincial Key laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical University Key Laboratory of Translation Medicine Tropical Diseases of Ministry of Education Hainan Medical UniversityHaikouHainanChina Department of Pathogen BiologyHainan Medical University Program in Public Health School of MedicineUniversity of CaliforniaIrvineUSA Department of Vector Control Centers for Disease Control and Prevention of Guangdong Province

Introduction:Aedes albopictus(Skuse) is an invasive mosquito that has become an important vector of chikungunya,dengue and Zika *** the absence of specific antiviral therapy and vaccine,vector management is the sole method available for reducing Aedes-induced disease *** the resistance status of *** to insecticides and to explorethe resistance mechanisms is essential for future vector control ***: *** larvae and pupae were sampled from six sites(two sites each from urban,suburban and rural) in *** resistance bioassays were conducted against Bacillus thuringiensis ***(Bti),deltamethrin,propoxur and malathion for larvae,and deltamethrin,DDT,propoxur and malathion for adults.P450 monooxygenase(P450 s),glutathione S-transferase(GSTs) and carboxylesterase(COEs) activities of adult mosquitoes were *** at the knockdown resistance(kdr) gene were analyzed,and association between kdr mutations and phenotypic resistance was ***: Adult bioassays revealed varied resistance levels against DDT and deltamethrin in the six *** populations with corrected mortalities ranging between 74 to 97% and 88 to 99%,*** mosquito populations showed higher resistance levels against DDT and deltamethrin than populations from suburban and rural *** populations tested were fully susceptible to propoxur and malathion except urban strain(UYS) and rural strain(RCD).Larval bioassays results indicated that all populations of *** were sensitive to the larvicide Bti and *** the populations showed resistance to deltamethrin and the average RR50 in urban,suburban and rural areas compared to the control is 27,14 and 18 fold,*** presence of F1534 S and F1534 L mutations was significantly associated with deltamethrinand DDT-resistant *** assays indicated that GSTs may play a role in DDT resistance in adult ***

关键词： Aedes albopictus Insecticide resistance Biochemical assay Kdr Urbanization Guangzhou

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Down-regulation of mmu-miR-181a-5p suppresses the microglia apoptosis induced by excretory and secretory products of Angiostrongylus cantonensis via Bcl-2/Bax

Down-regulation of mmu-miR-181a-5p suppresses the microglia ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Wei Y Wang HJ Liu JM Zhou XM Li H Department of Pathogen Biology and Experimental Teaching Centre of Preventive Medicine School of Public HealthSouthern Medical University

Objective Our previous study found that the mi R-181 a-5 p was down-regulated in the brain of mice infected with Angiostrongylus *** study is to verify the regulatory function of mir-181 a-5 p in mice *** The murine microglia were stimulated with excretory and secretory products(ESP) of Angiostrongylus cantonensis after transfection with mi R-181 a-5 p agomir and *** expression levels of mi R-181 a-5 p,target gene Bcl-2 and its conjugate Bax were detected by real-time quantitative PCR,Western blot and immunocytochemistry *** cell proliferation index was detected by flow *** In the microglia transfected with mi R-181 a-5 p agomir,the expression of mi R-181 a-5 p increased significantly(P<0.01).Accordingly,the m RNA and protein expression level of bcl-2 decreased(P<0.05) *** the contrary,transfected with mi R-181 a-5 p antagomir,the expression of mi R-181 a-5 p decreased significantly(P<0.01),and the m RNA and protein expression level of bcl-2 increased to(P<0.05).Besides,when the microglia were stimulated with ESP,the expression level of mi R-181 a-5 p similarly decreased(P<0.01),and the levels of bcl-2 m RNA and protein increased significantly(P<0.05) after ESP stimulation for 12 h,24 h,36 h and 48 *** ratio of bcl-2/bax was significantly decreased(P<0.05),and the apoptotic rate increased obviously(P<0.01) after ESP ***,the ratio of bcl-2/bax was significantly decreased(P<0.05),and the apoptotic rate of microglial cells increased(P<0.05) after transfected with mir-181 a-5 p agomir and stimulated with *** ratio of bcl-2/bax was significantly increased(P<0.05),and the apoptotic rate decreased(P<0.01) after transfected with mir-181 a-5 p antagomir and stimulated with *** Downregulation of mi R-181 a-5 p may promote the expression of bcl-2 in mice microglia and lead to the increase of the bcl-2/bax ratio,which could inhibit microglia apoptosis caused by the

关键词： Angiostrongylus cantonensis excretory and secretory products miR-181-5p bcl-2 apoptosis

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Prevalence of piroplasma in ticks from Guangxi,South China determined by reverse line blot hybridization assay

Prevalence of piroplasma in ticks from Guangxi,South China d...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Yali Sun Qin Liu Yi Zhang Lan He Min Hu Bang Shen Long Yu Junlong Zhao State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine Huazhong Agricultural University National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionKey Laboratory of Parasite and Vector BiologyMinistry of HealthWHO Collaborating Center for Tropical Diseases Key Laboratory of Animal Epidemical Disease and Infectious Zoonoses Ministry of AgriculturalHuazhong Agricultural University

Piroplasmosis,a disease of domestic and wild animals,is caused by tick-borne protozoa of the genera Theileria and *** leads to huge economic losses to the livestock *** disease has been frequently reported in subtropical and tropical regions ***,information on the prevalence of piroplasma in ticks is lacking in most *** assess the potential threat of piroplasmosis in South China,671 ticks were collected from Guangxi *** was extracted from these ticks to evaluate the presence of piroplasma through a reverse line blot(RLB) hybridization assay,which used the hyper-variable region 4 of the piroplasmic 18 S rRNA genes as probes to report Theileria and Babesia *** RLB results indicated that 144/671(21.46%) of the ticks were infected with piroplasma species belonging to the genera Theileria or *** buffeli,Babesia vogeli,***,and *** were present in ticks at frequencies of 60/671(8.94%),21/671(3.13%),6/671(0.89%),and 6/671(0.89%),***-infections with two or more piroplasma species were present in 8/671(1.19%) of the tick *** hyper-variable V4 region of the piroplasmic 18 S rRNA gene from six tick DNA samples with single infections was cloned,sequenced and aligned with related sequences from Gen *** and Babesia sequences were analysed *** and *** 18 S rRNA gene fragment sequences were compared with reported homologous sequences *** three *** species examined in this study were grouped into the same cluster and belonged to the same *** present study provides important epidemiological information for piroplasmosis occurrence in *** existence of tick-borne piroplasma likely leads to high infection risks for local animals in the studied areas.

关键词： Piroplasma Ticks Reverse line blot Piroplasmosis Theileria Babesia

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Prevalence and burden of Toxoplasma gondii infection in HIV-infected people: a systematic review and meta-analysis

Prevalence and burden of Toxoplasma gondii infection in HIV-...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ze-Dong Wang Huan-Huan Liu Shuang Li Li Zhang Feng Wei Xing-Quan Zhu 刘全 State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences Military Veterinary Institute Academy of Military Medical SciencesKey Laboratory of Jilin Province for Zoonosis Prevention and Control Central Laboratory Inner Mongolia General Forestry Hospital College of Life Science Jilin Agricultural University

Background: Worldwide,30% of the world's population have antibodies to the intracellular protozoan parasite Toxoplasma gondii and about 36.7 million people are infected with HIV,but little is known about the prevalence of co-infection with T gondii and *** aimed to characterise the epidemiology and burden of T gondii co-infection in people with HIV ***: In this systematic review and meta-analysis,we searched Pub Med,Embase,Google scholar,Science Direct,Chinese Web of Knowledge,Wanfang,and Chongqing VIP databases for studies reporting T gondii infection in HIV-infected people from inception to Feb 29,*** were included if they investigated people with HIV infection and presented data that allowed us to establish the prevalence of T gondii *** excluded reviews,repeated studies,or animal studies,as well as studies that provided the final results without raw data,had a sample size less than 30 people,had unclear diagnostic methods of T gondii infection,or that included populations with increased risk of T gondii *** extracted the numbers of patients with HIV infection and T gondii-coinfection from the identified *** estimated pooled prevalence of T gondii infection in HIV-infected people by a random-effects model,and evaluated its overall infection burden ***: Our search identified 7843 records and after removal of duplicates and initial screening,we reviewed 312 studies in *** these articles,238 were excluded,leaving 74 studies that included 25 989 HIV-infected people from 34 *** these people,7 326 had T gondii co-infection and we estimated the pooled worldwide prevalence of T gondii infection to be 35.8%(95% CI 30.8–40.7).2353 of 8837 of people in Asia and the Pacific had co-infection with T gondii and HIV(prevalence 25.1%,95% CI 19.0–31.2),and prevalence was low in this region compared with that in sub-Saharan Africa(44.9%,32.3–57.5,2129/5686;odds ratio [OR] 0.61),Latin America and the Cari

关键词： HIV Toxoplasma gondii Meta-analysis

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Identification of two novel host proteins interacting with Toxoplasma gondii 14-3-3 protein by yeast two-hybrid system

Identification of two novel host proteins interacting with T...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Fa-Cai Li Qing Liu Hany M.Elsheikha Wen-Bin Yang Jun-Ling Hou Xing-Quan Zhu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences College of Animal Science and Veterinary Medicine Shanxi Agricultural University Faculty of Medicine and Health Sciences School of Veterinary Medicine and ScienceUniversity of NottinghamSutton Bonington Campus College of Veterinary Medicine Northwest A&F University

Toxoplasma gondii deploys many effector proteins in order to hijack and manipulate host cell signalling pathways,allowing parasite colonization,subversion of immune responses and disease *** effector protein 14-3-3(Tg14-3-3) promotes parasite dissemination inside the body,by enhancing the migratory ability of infected microglia and dendritic *** both the mechanism of action,and the host targets of Tg14-3-3 effector is important because of their importance to the parasite's *** aim of the present study was to explore the function of Tg14-3-3 by utilizing the yeast two hybrid system(Y2 HS) to identify novel Tg14-3-3 interactors/substrates in host cells.A human c DNA library was screened using Tg14-3-3 as the ***14-3-3(RH strain,Type I) was cloned into the p GBKT7 vector and expressed in the Y2 HGold yeast *** bait protein expression was validated by Western blotting analysis,auto-activation and toxicity investigation compared with control(Y2 HGold yeast strain transformed with empty p GBKT7 vector).Two positive Tg14-3-3 interactors identified by this screening,h CG1821272 and e IF5 B(Eukaryotic translation initiation factor 5 B),were isolated and *** approach made it possible to gain a better understanding of the function of Tg14-3-3 in regulating host proteins involved in key cellular processes,such as translational initiation and cell migration.

关键词： Toxoplasma gondii Tg14-3-3 host-pathogen interaction protein-protein interaction yeast two-hybrid system

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A genome-scale CRISPR screen defines host dependency factors required by Toxoplasma gondii infection

A genome-scale CRISPR screen defines host dependency factors...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Hai-Xia Wei Shui-Zhen Wu Hong-Juan Peng Department of Pathogen Biology Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical University

Background: Toxoplasma gondii are obligate intracellular protozoa,and due to their small genomes and limited number of encoded proteins,they have to exploit host facors for entry,replication,and *** host proteins can be called as host dependency factors(HDFs).HDFs are dispensable for cellular viability yet critical for pathogen infection,which can be ideal targets for therapeutic *** previous research showed that host Rho A and Rac1 GTPases accumulated on the parasitophorous vacuole membrane(PVM),and they are indispensible for Toxoplasma gondii *** also has been reported that host hypoxia-inducible transcription factor 1(HIF-1)/hexokinase 2(HK2) can reprogram the host cell's metabolism to create an environment conducive for parasite replication at physiological oxygen *** gondii lacks the enzymes required for de novo arginine biosynthesis and has to exploit the arginine transported from host cell ***,the other information about the HDFs of Toxoplasma gondii is highly ***: The genes of human foreskin fibroblast(HFF) cells were comprehensively edited using lentiviral CRISPR-Cas9-sg RNA libraries,and then the lentivirus-treated cells were infected with Toxoplasma gondii for 9 *** survived cells were used for sg RNA *** sg RNA sequences matched genes are potential ***: The preliminary experiment showed that the cells in the lentivirus-treated group can survive longer than which in the untreated control group after both being infected by Toxoplasma gondii tachyzoites at a multiplicity of infection(MOI) of 1.21 days post-infection with Toxoplasma gondii,the remained cells in the lentivirus treated group were *** sequencing with the universal primers for sg RNAs,22 potential genes were *** of them with the highest reads is Casitas B-lineage lymphoma-b(Cbl-b) gene,which can negatively regulate C-type lectin receptor-mediated innate *** exact

关键词： Toxoplasma gondii CRISPR-Cas9 host dependency factors Casitas B-lineage lymphoma-b

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Host regulatory responses to Trichinella spiralis depend on regulatory T cells differentiated from CD4 T cells driven by activating dendritic cells

Host regulatory responses to Trichinella spiralis depend on ...

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中国动物学会寄生虫学专业委员会第十六次全国学术会议暨第七次国际寄生虫学学术研讨会

作者： Ximeng Sun Kai Guo Chunyue Hao Yuan Gu Jing Yang Yuli Cheng Xinping Zhu Department of Medical Microbiology and Parasitology School of Basic Medical SciencesCapital Medical University

Objectives: To study the host regulatory responses to Trichinella spiralis（***）,the differentiation characteristics of CD4 T cells during *** infection and the roles of excretory-secretory products（ES） on the polarization of regulatory T cells（Treg） were detected,especially Tregs induction during *** ***,the dendritic cells（DCs） are the master regulators of T cell responses for many infectious diseases,*** ES as an immunomodulatory agent,we hypothesized that ES could activate mouse bone marrow-derived dendritic cells（BMDCs） and increase ***: To analyze the impacts of *** infection on Treg induction,BALB/c mice were each infected with 400 infective *** muscle larvae by oral gavage,subsequently the levels of the CD4 subpopulations from mouse spleens in different stages of *** infection-intestinal phase（Day 6）,NBL migration（Day 15）,larva capsule（Day 30） and convalescent phase（Day 60）-were detected by fluorescence-activated cell sorting（FACS）.Meanwhile,Mouse BMDCs were incubated with ES products from *** adult worms（AES） and *** muscle larvae（MES） for *** of DC was determined by FACS *** cytometry was used to detect the presence of CD4CD25Foxp3 T cells after co-incubation of ES-activated BMDCs and na?ve CD4 T ***,the proportions of CD4CD25Foxp3 T cells or CD4CD25-Foxp3 T cells in ES-immunized mice splenocytes were also detected by Flow cytometry,and T cell receptor（TCR） pathway of FOXP3 gene expression was preliminarily determined by western blot and real-time polymerase chain reaction（PCR）.Cytokines were detected by and enzyme-linked immunosorbent assay（ELISA）.Results: After day 6 *** infection,the increased percentage of Th2 cells and Tregs（CD4CD25Foxp3 and CD4CD25–Foxp3） in CD4 T cells were observed,but not Th1 cells after 15 days ***,there was no significantly change that proportion of Th1,Th2 cells and Tregs

关键词： Trichinella spiralis CD4+ T cell dendritic cell regulatory T cell

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