哺乳动物卵母细胞成熟质量决定了早期胚胎能否正常发育.在临床上,对于有排卵障碍的病例而言,生发泡(Germinal Vesicle,GV)期卵母细胞体外成熟、是人类辅助生殖技术的重要组成部分,因此,筛选优良发育潜能的GV期卵母细胞具有重要临床意义.卵丘细胞层厚度(thickness of cumulus layers,C)、透明带厚度(thickness of zona pellucida,Z)和带下间隙宽度(perivitelline space,P)是卵母细胞形态学评价的三个重要参照物.本实验以小鼠为研究对象,对卵丘细胞层厚度、透明带厚度和带下间隙宽度进行分级,研究了这三个形态学特征与GV期卵母细胞质量的关系.更进一步,通过改变卵丘细胞层厚度、透明带厚度和带下间隙宽度,研究了这三个形态学指标作为评价卵母细胞发育潜能标准的可靠性.
In mammals, oocytes within the primordial follicles require a number of essential factors to maintain their ***, the survival factors for activated oocytes have been poorly *** we reported that damaged DNA binding pro...
In mammals, oocytes within the primordial follicles require a number of essential factors to maintain their ***, the survival factors for activated oocytes have been poorly *** we reported that damaged DNA binding protein-1 (DDB 1), the linker subunit of the cullin ring-finger ubiquitin E3 ligase-4 (CRL4) complex, and its substrate adaptor, DDB1-CUL4 associated factor-1 (DCAF1), were essential for primordial follicle *** this study we specifically deleted these in the oocytes of growing follicles, to investigate if DDB1 and DCAF1 were also survival factors for activated *** the ovaries of Ddb1fl/fl,;Zp3-Cre mice, the primordial follicle pool was intact, but awakened oocytes and growing follicles beyond the primary stage were rapidly *** the ovaries of Dcaf1fl/fl;Ptenfl/fl;Gdf9-Cre and Ddb1fl/fl;Ptenfl/fl;Gdf9-Cre mice, global primordial follicle activation was stimulated by enhanced PI3K signaling, but the awakened oocytes were rapidly lost due to no CRL4DCAF1 *** mouse models provided original evidence that CRL4DCAF1 was essential for maintaining oocyte survival, not only those in dormancy at the primordial follicle stage, but also naturally awakened oocytes and those awakened by hyper-activation of PI3K ***, the oocyte-specific Ddb1 or Dcaf1 knockout mice had ovulation defects even before oocyte ***4DCAF1 within oocytes was required for cumulus expansion and ovulation-related somatic gene expression in a cell non-autonomous *** cells that surrounded these Ddb1 or Dcaf1-deleted oocytes exhibited increased rates of apoptosis and showed poor responses to ovulation *** results suggested that CRL4 in oocytes also regulated granulosa cell functions in a cell non-autonomous manner.
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