Vitamins are a well-known group of compounds that are essential for human health and are classified into two main groups, water-soluble and fat-soluble. Reversed-phase HPLC is a well-suited technique for vitamin analy...
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Vitamins are a well-known group of compounds that are essential for human health and are classified into two main groups, water-soluble and fat-soluble. Reversed-phase HPLC is a well-suited technique for vitamin analysis. Commonly, water-soluble vitamins are determined using an aqueous mobile phase with low-organic solvent content, whereas fat-soluble vitamins are determined using mobile phases with high organic solvent contents.
N-methylcarbamates are widely used pesticides in agriculture. Reversed-phase high performanceliquid chromatography (RP-HPLC) with fluorescence detection following postcolumn derivatization,I.e. EPA Method 531.2,1 is t...
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N-methylcarbamates are widely used pesticides in agriculture. Reversed-phase high performanceliquid chromatography (RP-HPLC) with fluorescence detection following postcolumn derivatization,I.e. EPA Method 531.2,1 is the typically used method for the sensitive determination of carbamates,and Thermo Scientific has published a detailed method2,3 that is consistent with the EPA *** an HPLC with UV absorbance detection method is used, the sample preparation procedures ofliquid-liquid extraction or off-line solid phase extraction (SPE) is required to increase the detectionsensitivity;however, these procedures are time-consuming, require large volumes of organicsolvents, and are deficient in terms of process control.
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