The protein structural entries grew far slower than the sequence *** is partly due to the bottleneck in obtaining diffraction quality protein crystals for structural determination using X-ray *** first step to achieve...
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The protein structural entries grew far slower than the sequence *** is partly due to the bottleneck in obtaining diffraction quality protein crystals for structural determination using X-ray *** first step to achieve protein crystallization is to find out suitable chemical ***,it is not an easy *** trial and error tests of numerous
Protein-protein interaction is closely related with protein crystallization,and it changes along with changing of pH *** effect of pH on protein crystallization screening and reproducibility,and its correlative with p...
Protein-protein interaction is closely related with protein crystallization,and it changes along with changing of pH *** effect of pH on protein crystallization screening and reproducibility,and its correlative with protein-protein interaction were studied *** screening experiments were carried out by"dry drop method"(drops were mixed by precipitant regents from commercial screening kits,after drying,added protein solution with
It was reported that protein crystals of larger size and higher quality can be obtainedinspace environment.A superconducting magnet is thought one of the best ground-based simulation techniques at *** the first time,c...
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It was reported that protein crystals of larger size and higher quality can be obtainedinspace environment.A superconducting magnet is thought one of the best ground-based simulation techniques at *** the first time,crystals of lysozyme were obtained successfully from levitated containerless droplets in microgravity condition generated by the superconducting
Recognition of methylated histone tail lysine residues by tudor domains playsimportant roles inepigenetic control of gene expression and DNA damage *** studies revealed thebinding of methyllysine in a cage of aromatic...
Recognition of methylated histone tail lysine residues by tudor domains playsimportant roles inepigenetic control of gene expression and DNA damage *** studies revealed thebinding of methyllysine in a cage of aromatic residues,but the molecular mechanism by whichthe sequence specificity for surrounding histone tail residues is achieved remains *** the crystal structure of a trimethylated histone H3K4 peptide bound to
Nit proteins,as the branch 10 sub-family of nitrilase superfamily,are wildly dispersed in mammals,plants,fungi and even *** on sequence similarity,yeast Nit2 is one of the most recently discovered homolog of human ***...
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Nit proteins,as the branch 10 sub-family of nitrilase superfamily,are wildly dispersed in mammals,plants,fungi and even *** on sequence similarity,yeast Nit2 is one of the most recently discovered homolog of human *** to NitFhit tumor suppressor fusion protein,the homologue in fly and worm,human Nit1 is also a tumor suppressor but with additional *** this study,an apo structure,an alpha-ketoglutarate covalently binding
The lecture will give an introduction to the method(part 1)and examples for state-of-the-art applications(part 2).*** pioneering experiments performed hundred years ago,diffraction has developed to maturity.X-rays int...
The lecture will give an introduction to the method(part 1)and examples for state-of-the-art applications(part 2).*** pioneering experiments performed hundred years ago,diffraction has developed to maturity.X-rays interact with electrons;therefore,all scattering experiments with this kind of radiation in principle determine the electron density in a *** experiments can
In proteins,conformational change impacting the function is well-investigated in the past decades,which was named‘Allostery’.But in DNA-protein interactions,the DNA affects a DNA binding protein only by‘allosterism...
In proteins,conformational change impacting the function is well-investigated in the past decades,which was named‘Allostery’.But in DNA-protein interactions,the DNA affects a DNA binding protein only by‘allosterism’(different conformations)without sequence changed is not well ***,it could exist in nucleosome,as Nucleosomal DNA is much less flexible and accessible than free DNA as its bending,fixing and blocking by histone
With the virtue of its symmetry selectivity optical second harmonic generation(SHG)is particularly attractive for probing the ferroelectric *** second-order nonlinear optical polarization is given by P(2ω)=ω(2)E2(ω...
With the virtue of its symmetry selectivity optical second harmonic generation(SHG)is particularly attractive for probing the ferroelectric *** second-order nonlinear optical polarization is given by P(2ω)=ω(2)E2(ω)with electric fields E(ω)of the incident light *** the second-order susceptibilityω(2)ω0 for noncentrosymmetric systems only,SHG effect directly couples to the symmetry-breaking ferroelectric order ***,under the external stimuli conditions
Rational designed nucleases for gene editing(DNA targeting)have become feasible toolsdue to the application of TALEN(TAL Effector Nuclease)*** are engineered proteins composed of easy designableDNA binding domains of ...
Rational designed nucleases for gene editing(DNA targeting)have become feasible toolsdue to the application of TALEN(TAL Effector Nuclease)*** are engineered proteins composed of easy designableDNA binding domains of bacterial transcription activator-like effector(TALE),and FokI DNA-cleavage *** consist of individual near identicaltetratricopeptide repeat(TPR)units of mainly 34 residues with repeat-variable diresidue
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