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干旱胁迫对防风二年生苗根系次生代谢产物的影响

干旱胁迫对防风二年生苗根系次生代谢产物的影响

引用

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者：焦苗苗门玉倩陈贵林内蒙古大学牧草与特色作物生物技术教育部重点实验室内蒙古自治区中蒙药材规范化生产工程技术研究中心生命科学学院

药用植物的药效成分多为植物的次生代谢产物,包括生物碱、黄酮、萜类、酚类、皂苷等。防风(Saposhnikovia divaricata (Turcz.) Schischk)为伞形科植物未抽花茎植株的干燥根,是我国常用大宗中药材之一,一般种植在干旱、半干旱地区。防... 详细信息

药用植物的药效成分多为植物的次生代谢产物,包括生物碱、黄酮、萜类、酚类、皂苷等。防风(Saposhnikovia divaricata (Turcz.) Schischk)为伞形科植物未抽花茎植株的干燥根,是我国常用大宗中药材之一,一般种植在干旱、半干旱地区。防风生长期常常会受到不同程度的干旱胁迫,其干旱胁迫对防风植株生物量和产量影响如何?尤其是对防风次生代谢产物积累代谢有何影响还不清晰。试验于2016年4月到2017年6月在呼和浩特市内蒙古大学南校区温室进行(40°45′N,111°40′E,海拔1040 m),采用盆栽试验(容器规格:上口径18 cm,下口径10.5cm,高25.5cm),土壤为田园土。实验设置了对照,干旱(干旱处理14天)和复水(干旱14天后恢复浇水12天)3个处理。分别测定防风生物量变化,色原酮类成分(主要为升麻素苷和5-O-甲基维斯阿米醇苷)、挥发性成分等次生代谢产物含量的变化,探讨干旱胁迫对二年生防风苗生理生化及次生代谢产物积累的影响。结果表明,持续干旱胁迫14天后,土壤含水量显著降低,与对照相比降低了78.31%。干旱后防风苗叶片萎蔫发黄,叶面积减少,地上部干重降低,而根系干重显著增加,与对照相比增加了42.60%。升麻素苷和5-O-甲基维斯阿米醇苷是药典规定的防风质量分析的指标性成分,与对照相比,干旱胁迫后两种成分含量均显著增加,分别增加了33.11%和63.78%。而且,在各处理条件下,升麻素苷和5-O-甲基维斯阿米醇苷的总含量分别为:对照组0.38%;干旱组0.62%;复水组0.76%,均超过药典所规定的最低含量0.24%的要求,表明实验所用的防风苗品质较好。通过GC-MS分离和鉴定了不同处理条件下防风根系挥发油的化学成分,共检测出了14种挥发性成分,其中倍半萜类化合物4种,芳香醇类化合物7种,酯类化合物、杂环化合物、酚类化合物各一种,芳香醇类化合物占据61%。在干旱条件下,虽有部分挥发性成分的含量有所增加,如β-柏木萜烯、β-Barbatene和β-没药烯等,但总的来说,各挥发性成分相对含量受干旱胁迫的影响效应不一致。因此,对于本实验我们可以得出以下结论,适度的干旱胁迫能增加防风根系生物量,显著增加升麻素苷和5-O-甲基维斯阿米醇苷含量,但对于挥发性成分的影响无明显趋势。

关键词：防风生物量色原酮挥发性成分

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贵州石笔木种子萌发的内源抑制物质及作用研究

贵州石笔木种子萌发的内源抑制物质及作用研究

引用

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者：郭米香刘海燕吕晓梅邹天才贵州大学林学院贵州省植物园贵州科学院

贵州石笔木Tutcheria kweichowensis Chang et ***为常绿乔木,树形美观,材质优良,平均果重65.6g,果仁含脂肪61.7%、纯蛋白20.19%,果肉富含茶多酚、VE、VC、总糖等,是重要速生经济林木。自然分布于28°N的贵州高原亚地区（ⅢD10d）... 详细信息

贵州石笔木Tutcheria kweichowensis Chang et ***为常绿乔木,树形美观,材质优良,平均果重65.6g,果仁含脂肪61.7%、纯蛋白20.19%,果肉富含茶多酚、VE、VC、总糖等,是重要速生经济林木。自然分布于28°N的贵州高原亚地区（ⅢD10d）赤水桫椤国家级自然保护区,自然繁殖率低、Meta-种群生态特征明显,已成为珍稀濒危植物。利用石油醚、乙醚、乙酸乙酯和甲醇为浸提溶剂,采用系统分离法得到种子、种皮、胚乳各有机相的浸提液,对浸提液中的物质进行浓缩后,经气相色谱―质谱联用仪（GC―MS）鉴定分析其抑制物质成分及含量。用不同部位、溶剂、温度和浓度处理的浸提液浸种白菜种子,检测其抑制物质的生理活性及对白菜种子萌发的影响。结果表明:（1）贵州石笔木种子内源抑制物质主要存在于种皮和胚乳中,在种皮和胚乳中的含量分别为有机酸类80.49%、95.95%(油酸CHO、亚油酸CHO、十六烷酸CHO、乙酸CHO、十八烷酸CHO、2-甲基丁酸CHO、2-甲基巴豆酸CHO),烯类10.37%、1.59%(三十碳六烯CH),醛类2.92%和0.26%(5-（羟甲基）糠醛CHO、糠醛CHO、乙醇醛CHO),酯类2.83%、0.68%(2-羟基-γ-丁内酯CHO、单乙酸甘油酯CHO、亚油酸甲酯CHO、油酸甲酯CHO、甘油二乙酸酯CHO),醇类2.34%、0.67%(丙酮醇CHO、糠醇CHO、1,3-丁二醇CHO),酚类1.03%、0.84%（苯酚CHO）。（2）种皮中酸类有机化合物相对含量总数为80.49%,胚乳中酸类有机化合物相对含量总数为95.95%,但由于胚乳中含有的油酸和亚油酸偏高,而使烯类、酯类、醇类、醛类、酚类有机化合物的相对含量偏低,因此,在贵州石笔木种子萌发的过程中,种皮中抑制物质起到的影响作用较大。（3）种皮浸提液抑制作用最大,种子浸提液次之,胚乳浸提液的抑制作用最小,具有显著差异（P<0.01,P<0.05）。（4）各个处理的浸提液均显著抑制白菜Brassica pekinensis Rupr.种子的发芽率、主根长和幼苗高,浸提液抑制物活性表现为醇溶剂大于水溶剂,并随着浸提液浓度的升高而增大、随着浸提温度的升高表现为先增大后减小,在30℃时达到最大值,分别为12.5%、81.1%、27.74%。（5）浸种的初始温度为100℃时,抑制物质活性达到最大值,白菜种子萌发率显著低于60℃～90℃处理的白菜种子（P<0.01,P<0.05）。在贵州石笔木种子播种前,可用初始温度为100℃的水中进行多次浸种,使种子内源抑制物质更多的溶于水中,可有效降低其自身萌发过程中的抑制作用,更好的促进种子的萌发和生长。

关键词：种子萌发内源抑制物质有机化合物部位含量及作用贵州石笔木

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唐古特白刺类黄酮3-O-葡萄糖基转移酶基因(UTGT)的功能分析

唐古特白刺类黄酮3-O-葡萄糖基转移酶基因(UTGT)的功能分析

引用

中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者：汤宇晨王佳王迎春内蒙古大学牧草与特色作物生物技术教育部重点实验室

强旱生盐生植物唐古特白刺(Nitrariaria tangutorum Bobr.)为中国特有的蒺藜科白刺属多年生灌木,具有极强抗逆能力,是土壤荒漠化和盐碱化防治的先锋植物,对维持沙漠生态平衡和防止盐碱化具有重要意义。在植物中,类黄酮3-O-葡萄糖基转移... 详细信息

强旱生盐生植物唐古特白刺(Nitrariaria tangutorum Bobr.)为中国特有的蒺藜科白刺属多年生灌木,具有极强抗逆能力,是土壤荒漠化和盐碱化防治的先锋植物,对维持沙漠生态平衡和防止盐碱化具有重要意义。在植物中,类黄酮3-O-葡萄糖基转移酶(UFGT)将不稳定的花色素转变为稳定的花色素苷,是花色素苷形成的关键酶,植物可通过积累花青素来抵抗逆境胁迫。本研究从唐古特白刺中克隆花青素合成途径中的关键基因类黄酮3-O-葡萄糖基转移酶NtUFGT,分析其序列信息、系统进化及表达特性,同时构建真核表达载体,探讨其在拟南芥抵抗非生物胁迫过程中发挥的作用。通过对唐古特白刺转录组测序结果分析,从中成功克隆UFGT基因的一个开放阅读框(NtUFGT),利用生物学软件和数据库,对该基因的结构进行生物信息学分析。其ORF长1407bp,编码468个氨基酸组成的蛋白质,理论分子量为51.37kDa,理论等电点pI为5.77。NtUFGT基因编码的氨基酸序列与山竹子、荔枝、桑树和葡萄的同源性为58%,与拟南芥的同源性为56%。利用qRT-PCR技术对NtUFGT基因在唐古特白刺不同组织及发育过程中果实的表达情况进行分析,该基因在根茎叶中的表达较低,在花中表达最高,这可能与花中花青素合成有关。在果实中的表达量也较根茎叶中高,并且随着果实的发育,该基因的表达呈明显升高趋势,表明在果实发育的后期NtUFGT基因对果实中花青素的合成积累具有重要作用。构建pPZP221-NtUFGT真核表达载体,通过农杆菌侵染法转化拟南芥,过量表达NtUFGT基因,验证其生物学功能。对T3代NtUFGT转基因拟南芥在水分胁迫、NaCl胁迫下进行根长、鲜重、叶绿素含量等生理指标测定,发现转基因拟南芥生长状况均优于野生型。不同处理下拟南芥中抗氧化酶SOD、POD、CAT的活性升高,H2O2及MDA含量显著降低。以及检测了拟南芥中花青素、原花青素、总黄酮的含量,证实NtUFGT的过表达能诱导花青素大量积累。这些结果表明转入NtUFGT能促进拟南芥中花青素的合成,提高拟南芥抗氧化能力,减轻了膜损伤程度,从而改善了转基因植株在干旱和盐胁迫环境下的生长状况,增强其抗逆性。证实了NtUFGT的过表达和花青素的积累对拟南芥在应对非生物胁迫的过程中发挥了积极的作用。

关键词：唐古特白刺花青素非生物胁迫 UFGT基因抗氧化

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凋落叶中木质素的降解特征及其微生物影响机制——以亚热带山地常绿落叶阔叶混交林优势植物小花木荷和亮叶水青冈为例

凋落叶中木质素的降解特征及其微生物影响机制——以亚热带山地常...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者：贺美田秋香赵汝东王兴刚刘峰中国科学院武汉植物园水生植物与流域生态重点实验室中国科学院大学

木质素是地球上含量最丰富的芳香族化合物,是决定凋落物质量好坏及分解快慢的关键指标。凋落物质量及土壤微生物是影响凋落物分解的主要因素,因森林群落植被组成差异而变化,明确它们对凋落叶木质素单体降解的影响对森林生态系统碳代谢... 详细信息

木质素是地球上含量最丰富的芳香族化合物,是决定凋落物质量好坏及分解快慢的关键指标。凋落物质量及土壤微生物是影响凋落物分解的主要因素,因森林群落植被组成差异而变化,明确它们对凋落叶木质素单体降解的影响对森林生态系统碳代谢与循环有重要意义。选取湖南省八大公山国家自然保护区核心的主要优势群落亮叶水青冈(***)和小花木荷(***),设置***和***凋落叶(初始木质素含量差异显著)分别接种***和***群落表层土壤微生物的4个处理,利用CuO氧化-气相色谱法追踪木质素酚单体香草基(V)、丁香基(S)和肉桂基(C)的变化,磷脂脂肪酸法(PLFAs)分析微生物群落动态。结果表明,经过半年的分解,***和***凋落叶质量平均损失率分别为15.4%和27.9%,无论接种哪个群落微生物,***凋落物降解率均显著高于***(P<0.05);重复测量方差分析表明,凋落物分解受凋落物质量(P<0.0001)和培养时间(P<0.0001)及它们交互的显著影响(P<0.05),土壤微生物对其无显著影响。经过半年降解,***和***木质素平均损失率为22.2%和18.5%;无论接种哪个群落微生物,*** S单体损失率均显著高于***(P<0.05),C单体损失率均显著低于***(P<0.05);***木质素和C单体降解均表现出主场优势效应;木质素及S单体浓度均仅受凋落物质量显著影响(P<0.0001),C单体浓度受凋落物质量(P<0.0001),时间(P<0.0001),凋落物质量与微生物(P<0.0001)及与时间交互(P<0.05)的共同影响。接种***群落土壤微生物后,*** V单体损失率显著高于*** (P<0.05),接种***群落土壤微生物,二者则无显著差异;与主场优势相反,*** V单体损失率接种外源微生物显著高于接种本土微生物3.8%(P<0.05);V单体浓度受凋落物质量(P<0.0001)和时间(P<0.01)的显著影响。综合各分解时期PCA分析,G+/G-与木质素及各单体含量始终显著相关,真菌,真菌细菌比和真菌放线菌比与S单体和V单体酸醛比均显著相关,表明真菌在木质素单体降解中的重要作用。本研究通过互换凋落叶和土壤接种液的实验初步了解凋落物质量和微生物在木质素降解过程中的相对重要性,发现凋落物质量在决定木质素各单体初期分解速率方面比土壤微生物扮演更加重要的角色。

关键词：凋落叶分解木质素单体微生物主场优势

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The effect of winter chilling temperature on light-energy utilization and dissipation in eight tropical tree species planted in a southern subtropical city

The effect of winter chilling temperature on light-energy ut...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： John Sunoj Valiaparambil Sebastian Yin Wen Aidan William Short Wenhao Zeng Kunfang Cao State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources Guangxi University Guangxi Key Laboratory of Forest Ecology and Conservation College of ForestryGuangxi University

Photosynthesis of tropical tree species is highly sensitive to chilling temperature,consequently limiting their movement to higher latitudes in facing to global *** southern subtropical cities,tropical plants are cultivated for ornamental purpose,where short-term chilling temperature incidents(≤10°C for few days) can occur during *** a chilling period,the photosynthesis and photochemical efficiency of tropical plants may be reduced,but their non-photochemical quenching may be increased to dissipate excessive light *** study was conducted to investigate the efficiency of photosystems and role of photo-protection in tropical tree species to survive in such unfavorable growth *** diverse eight tropical tree species grown in an open field were selected for this *** exchange,photochemical efficiency of photosystem II(PSII) and redox state of PSI,cyclic electron flow(CEF) and chlorophyll index were measured in winter and *** results revealed that four out of eight selected species showed significant reduction in photosynthetic rate(PN),carboxylation capacity(PN/Ci),photochemical efficiency of PSII in the dark adapted state(Fv/Fm) and photochemical efficiency of PSII in the light adapted state(Fv'/Fm') in ***,an increase in maximum efficiency of PSI in the light adapted state(Pm') in winter was observed in all species;and changes in chlorophyll index shown a mixed *** non-photochemical quenching(NPQ) and cyclic electron flow(CEF) were activated in summer as compared to the winter in all species except CEF in Woodfordia fruticose *** was higher in both seasons along with a higher PN and PN/Ci than other species;and activation of NPQ and CEF in summer can be attributed to the high intensity of *** results from the current study specifies the important role of CEF in maintaining the physiological activities in the most chilling tolerant tropical tree species Woodfordia fruticose L.

关键词： Tropical tree photosynthesis cyclic electron flow chilling temperature photochemical efficiency

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Rain-shelter cultivation improves the vegetative and reproductiv characters of cherry(Prunus pseudoceresus) in Southwest China

Rain-shelter cultivation improves the vegetative and reprodu...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Tian Tian Xiaopeng Wen Guang Qiao Bin Deng Zhuang Wen Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Institute of Agro-bioengineering/College of ForestryGuizhou University Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) Institute of Agobioengineering/College of Life ScienceGuizhou University

In the whole stage of blossom and fruit development,hail and rain can cause yield losses of cherry(Prunus pseudoceresus) up to 90% under the traditional shelter-free cultivation in southwestern ***,the rain-shelter cultivation was increasingly applied to prevent flower and fruit from being damaged by excessive rainfall as well as hail in Southwest ***,whether the rain-shelter cultivation severely affects the growth characteristic and fruit quality of cherry had not yet been fully clear so ***,the 5-year-old field-grown plants were subjected to the trials as covered with polyethylene film from early phase of flowering to fruit-picking ends for one year(1 a-shelter) and two years(2 a-shelter).The results demonstrated that width,diameter of leaves in shelter were superior to those in *** rain-shelter cultivation highly increased the content of chlorophyll and *** shelter trial somewhat reduced the net photosynthetic rate,and it enhanced the total organic matter because no noon-slump in photosynthetic rate was investigated from the *** practice significantly reduced the dropping rate and cracking rate(to 5%) of fruit in comparison to that of shelter-free(92%),thus remarkably increased the yield by over ***,the average fruit weights of the 1 a-shelter(6.7 g) and 2 a-shelter(6.4 g) trial were significantly higher than that of the shelter-free(5.7 g),and average total soluble solid content(SSC) of the 1 a-shelter(12.4%) and 2 ashelter(11.6%) were also highly enhanced in comparison to those of the shelter-free,however,no significant differences in fruit soluble sugar content were observed between the shelter and ***,the rain-shelter cultivation during the flowering and fruit development stages didn't significantly affect the daily photosynthetic efficiency and fruit quality,and substantially enhance fruit yield,which may be helpful for the improvement of cher

关键词： rain-shelter Prunus pseudoceresus vegetable growth fruit quality cracking rate yield Southwest China

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Economic plant resources of Lal Suhanra national park of bahawalpur, pakistan: anthropogenic factors and conservation strategies

Economic plant resources of Lal Suhanra national park of bah...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Hafiz Muhammad Wariss Wei-Bang Sun Yong Peng Ma Lin-Lin Zhao Shazia Anjum Saeed Ahmad Khurshid Alam Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations Kunming Institute of BotanyChinese Academy of Sciences Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of Sciences University of Chinese Academy of Sciences Cholistan Institute of Desert Studies TheIslamia University of Bahawalpur University College of Agriculture University of Sargodha Department of Forestry Rang & Wildlife ManagementTheIslamia University of Bahawalpur

The Lal Suhanra National Park(LSNP) with its 65790.36 hectares surface area is located on southeastern part of the Punjab Province of *** distinguished by its unique topography and habitat heterogeneity,is comprised of three different ecosystems namely irrigated forest plantation,desert area/dry land and Pati Sir Lake(Pond area).Due to high diversity of wildlife,microhabitats and natural vegetation,picnic spots and fishing in the pond area,in 1972 it was designated as Biosphere Reserve Protected *** vegetation is threatened by expanding agriculture,deforestation and climate *** presented an ethnobotanical inventory among local communities and discuss the effects of vegetation change on the availability of useful *** organized expeditions to explore the whole area and collected plant *** used semi-structured,in-depth interviews to gather the ethnobotanical information about plant *** LSNP flora consists of 212 species belonging to 162 genera and 50 *** was the leading family that contributed 43 grasses,followed by Fabaceae(16 spp.),Asteraceae(15 spp.),Chenopodiaceae(10 spp.).We encountered 126 useful plants,Poaceae was the most species-rich family in economic value,followed by Asteraceae,Chenopodiaceae,and *** studies revealed that the inhabitants are very well familiar with diversity of the medicinal plant and now the use of these diversified medicinal plants has been extended even to the national *** has become a common treatment for their ailments on the one hand and also serves the purpose of their everyday requirement of food,fodder,shelter,fuel wood and *** was observed that the local people were least concerned about the conservation status of these natural resources and exploit them ruthlessly even sometimes to the level of their ***,the study area is facing a severe threat of anthropogenic interference that is entirely destructive for the endemic plant spec

关键词： National park plant diversity economic value anthropogenic factors conservation

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MYB regulator FhPAP1 from Freesia hybrida shows higher transactivation property compared with homologs from core eudicot plants Arabidopsis and tabacco in the biosynthesis of anthocyanins

MYB regulator FhPAP1 from Freesia hybrida shows higher trans...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Yueqing Li Xiaotong Shan SongYang Ruifang Gao Taotao Han Liudi Zhou Tingting Bao Shucai Wang Xiang Gao Li Wang Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology Northeast Normal University

The emergency of anthocyanin biosynthetic pathway has played vital roles in plant development,MYB regulators involved has been reported to arise early during land plant *** the division of monocots and dicots,they might function divergently suffered from diverse selection *** regulation mechanisms were found in dicot and monocot plants represented by Arabidopsis thaliana and Zea mays,*** far,amounts of anthocyanin-promoting R2 R3-MYBs have been identified and well elucidated in core dicot plants,whereas they were studied randomly in monocot plants,which are of crucial importance to decipher functional diversities among angiosperms during *** this study,one R2 R3-MYB gene FhPAP1 leading to the accumulation of anthocyanin was isolated from flowers of one monocot plant,Freesia *** and spatial expression profiles revealed FhPAP1 showed positive correlations with anthocyanin biosynthetic genes especially the late biosynthetic *** analysis indicated that it could form sets of MBW(MYB-bHLH-WD40) complexes with FhTT8 L,FhGL3 L and FhTTG1 ***-of-function experiments in Arabidopsis and Nicotiana and transient protoplasts transfection assays in Freesia further proved that FhPAP1 was sufficient to interacted with the bHLH proteins to regulate the expression of anthocyanin biosynthetic ***,other partners in the MBW complex,i.e.,WD40 regulators,could strengthen the transcription activation ***,FhPAP1 showed higher transactivation property when compared with AtPAP1 and NtAN2 from dicot plants Arabidopsis and tobacco,which resulted in different increments of target gene expression ***,it can be deduced that the MYB regulator from Freesia hybrida might have similar target genes and function conservatively compared with core eudicot plants,but its significant transactivation property will privilege it in crop improvement and also shed light on the mechanisms of anthocyani

关键词： Anthocyanin MYB regulator MBW complex transactivation property angiosperm

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Over-expression of GmKR3, a TIR-NBS-LRR type R gene, confers resistance to multiple viruses in soybean

Over-expression of GmKR3, a TIR-NBS-LRR type R gene, confers...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Hongwei Xun Xiangdong Yang Hongli He Meng Wang Peng Guo Ying Wang Jinsong Pang Yingshan Dong Xianzhong Feng Shucai Wang Bao Liu Key Laboratory of Molecular Epigenetics of MOE & Institute of Genetics and Cytology Northeast Normal University Jilin Academy of Agricultural Sciences Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of Sciences

Soybean mosaic virus(SMV) is found in many soybean producing areas,and SMV infection is one of the prevalent viral diseases that can cause significant yield losses in *** plants,resistance(R) genes are involved in pathogen reorganization and innate immune response *** R proteins have nucleotide-binding site and leucine-rich repeat(NBS-LRR) domains,and some of the NBS-LRR type R proteins in dicots have TIR(Toll/Interleukin-1 Receptor) *** report here the analysis of the over-expression of Gm KR3,a soybean TIR-NBS-LRR type R gene on virus resistance in *** over-expressed in soybean,GmKR3 enhanced the plant's resistance to several strains of SMV,the closely related potyviruses bean common mosaic virus(BCMV) and watermelon mosaic virus(WMV),and the secovirus bean pod mottle virus(BPMV).Importantly,over-expression of GmKR3 did not affect plant growth and development,including yield and the qualities of the *** analysis showed that abscisic acid(ABA) content increased in the 35 S:GmKR3 transgenic plants,and in both wild-type and 35 S:GmKR3 transgenic plants in response to virus *** with this observation,we found that the expression of two ABA metabolism genes was down-regulated in 35 S:GmKR3 transgenic *** also found that the expression of Gm04.3,an ABA responsive gene encoding BURP domain-containing protein,was up-regulated in 35 S:GmKR3 transgenic *** together,our results suggest that overexpression of Gm KR3 enhanced virus resistance in soybean,which was achieved at least in part via ABA signaling.

关键词： GmKR3 virus biotic stress ABA soybean

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Study on the extraction and activity of the active components of Cajanus cajan

Study on the extraction and activity of the active component...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Yu Kong Zuofu Wei Peng Wang Sundong Zhang Yujie Fu Key Laboratory of Forest Plant Ecology Ministry of EducationNortheast Forestry University Engineering Research Center of Forest Bio-preparation Ministry of EducationNortheast Forestry University

Pigeon pea [Cajanus cajan(L.) Millsp.],a member of the Cajanus genus,is one of the most valuable perennial or annual leguminous food crops in Asia,Africa and some parts of South America,and plays an important role in the field of agriculture and *** addition to being an agricultural and food resource,many folk medicinal uses for antibacterial,antioxidant,anti-inflammatory,abirritative and inhibition of capillary permeability activities have also been *** our study,an enzyme pretreatment and negative-pressure cavitation extraction(E-NPCE) method was investigated for extraction genistein and apigenin from pigeon pea roots,Under optimal conditions,the yields of genistein and apigenin were 0.628 mg/g and 0.359 mg/g,which represented an increase of 44.70% and 53.05%,respectively,compared to standard *** Supercritical fluid carbon dioxide(SF-CO2) extraction(SFE) was also developed and investigated for the extraction of Pigeon pea [Cajanus cajan(L.) Millsp.].The optimum extraction yields of CO2 flow rate,modifier composition and liquid to solid ratio were firstly optimised(12 kg/h,and 80% EtOH with a liquid to solid ratio of 10 ml/g raw material,respectively),and then the optimal parameters of extraction pressure,temperature and time on the extraction yields of CSA and PI were 30 MPa,60 °C and 2 *** pharmacological activities study demonstrated that cajanin stilbene acid(CSA) inhibited breast cancer cells by DNA damage and cell cycle-related signaling pathways leading to cell cycle arrest and apoptosis,along with the inhibited Bcl-2 expression and induced Bax expression to desintegrate the outer mitochondrial membrane and causing cytochrome c release by activation caspase-3 *** addition,previous study also showed cajaninstilbene acid(CSA) exerts cytoprotective effects against oxidative stress through the Nrf2-dependent antioxidant *** the best of our knowledge,it provides a material basis for the development of high efficiency and low toxic

关键词： Enzyme pretreatment and negative-pressure cavitation extraction Cajanus cajan(L.) Millsp.Cajaninstilbene acid.Anticancer Antioxidant

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