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OsPhtl;8,a rice phosphate transporter,regulates plant innate immunity and plant growth

OsPhtl;8,a rice phosphate transporter,regulates plant innate...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Dong Zheng Liu Jing Li Wei Dai Liangying Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and College of Plant Protection Hunan Agricultural University

The disease resistance and absorption of inorganic nutrients are two essential physiological activities in ***,it is still unclear whether there is cross talk between their molecular signaling ***12,a rice mitogen-activated protein kinase,is a positively regulator of plant innate *** this study,we identified OsPht1;8,a member of phosphate transporters(PTs) Pht1 family in rice(Oryza sativa),as an interactor of OsMPK12 and characterized the functions of OsPht1;*** low phosphate(Pi) conditions,the transcription level of OsPht1;8 is induced obviously and the OsPht1;8 over-expression plants show shorter root length and higher plant height than the non-transgenic ***,the differences in development between OsPht1;8 over-expression plants and the non-transgenic plants are reduced upon the increase of Pi concentration in growth ***,we found over-expression of OsPht1;8 suppresses rice disease resistance against the pathogens Magnaporthe oryzae and Xanthomonas oryzae *** which cause rice blast and bacterial leaf blight disease,***,the transcription level of the resistance related genes,such as OsPR10 and OsNPR1,and OsMPK12 are inhibited in OsPht1;8 over-expression plants after inoculation of the above mentioned *** the OsMPK12 gene is also suppressed,whereas,the OsPht1;8 gene is induced obviously after the pathogens *** addition,we also found OsPht1;8 is involved in regulating the PAMP flagellum and chitin triggered immunity(***).These results demonstrate that OsPht1;8 is a integrated regulator that it is involved in transduction of Pi signaling for development and negatively regulates rice innate immunity.

关键词： OsPhtl 8,a rice phosphate transporter,regulates plant innate immunity and plant growth

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Metabolomic profiling revealed general and specific chemical elicitors of rice defense response against Magnaporthe oryzae

Metabolomic profiling revealed general and specific chemical...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Justice Norvienyeku Lin Lili Sami Rukaiya Aliyu Chen Xiaomin LinLianyu Franklin Jagero ZhouJie Wang Zonghua Fujian University Key Laboratory for Plant-Microbe Interaction the School of Life SciencesCollege of Plant ProtectionFujian Agriculture and Forestry University State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops College of Plant ProtectionFujian Agriculture and Forestry University Minjiang University

Generally,plants exposed to persistent irritations from microbial pathogens,insect pest,parasitic plants and unfavorable environmental factors(biotic and abiotic stress) tend to generate and accumulate a host of phytochemicals(secondary metabolites) in different tissues as reactive chemicals to ward-off or kill invading pathogens and *** secondary metabolites also serve as a signaling cue for initiating hormonal signaling processes necessary for mitigating their successful adaptation and subsequent survival under harsh environmental *** metabolomics studies identified metabolomics divergence between japonica and indica rice *** insights gained from population genetic studies as well as rice blast disease epidemiology studies showed that japonica and indica rice species exhibit different levels of resistance and susceptibility against cosmopolitan and the most economically destructive rice blast pathogen ***,the impact of metabolomics differentiations on differential resistance of different rice cultivars from japonica and indica species against *** is still not well *** this study,we used UPLC-TOFF mediated non-targeted whole metabolome profiling techniques to investigate metabolomics divergence prevailing between whole leaf tissues and leaf trichome of four homogeneously susceptible and five differentially resistant rice cultivars from both japonica and indica *** also monitored differential *** mediated metabolomics reprogramming and reconstitution events occurring in respective rice cultivars during host-pathogen *** study identified set of metabolites that functions as phytochemicals in susceptible and resistant rice species under infection,among the disease resistance related phytochemicals identified exclusively resistant rice seedlings inoculated with the blast fungus includes,sakuranin(sakuranetin) and other reactive *** leaf trichome metabolome an

关键词： Phytochemical Saponin Metabolomics reprogramming UPLC-TOFF

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Biological activity of pterostilbene against Peronophythora litchii,the litchi downy blight pathogen

Biological activity of pterostilbene against Peronophythora ...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Xu Dandan Deng Yizhen Xi Pinggen Gao Lingwang Jiang Zide Department of Plant Pathology Guangdong Province Key Laboratory of Microbial Signals and Disease ControlSouth China Agricultural University College of Plant Protection China Agricultural University

Peronophythora litchii,the oomycete pathogen of litchi downy blight,infects leaves,flowers and fruits of litchi,leading to great economic loss during fruit riping,storage and ***,the new effective,environment-friendly fungicide(s) is urgently *** this study,eight phenolic compounds which exhibited strong bioactivity on Botrytis cinerea were selected to test their effectiveness on the inhibition of ***,which conducted by treating sporangia suspension with different tested phenolic compounds at the concentration of 10 and 50 mg/*** results indicated that pterostilbene showed strongest antifungal activity on sporangia germination among all tested compounds,which substantially suppressed sporangia germination of ***,with inhibition rate of 100%in the treatment of pterostilbene at 32 mg/L,and its IC value on zoospore release was evaluated to be 6.8 mg/***,Sytox green uptake assay and ultrastructure observation were used to explore to the action mechanism of pterostilbene on the inhibition of ***,the results showed that pterostilbene significantly inhibit the mycelial growth and induced deleterious morphological modifications and ultrastructural alteration,including deformation and shrinking of mycelia and sporangia,the damage of cell wall,plasma membrane and *** fruit decay and peel browning caused by *** during postharvest storage or transportation are two major factors affecting the commercial value of litchi fruit,and the effectiveness of pterostilbene on litchi downy blight control was *** assay demonstrated pterostilbene application significantly reduced the decay of litchi fruit and leaf and peel browning caused by ***,with the decay index reduction up to 48.6%and peel browning of litchi reduced from 3.5 to 1.8 after treated with 1 600 mg/L *** research provides further proof that pterostilbene is a potential and better candidate agent for antifungal agrochem

关键词： Litchi postharvest rot pterostilbene antifungal activity peel browning

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Screening and functional analysis of GSTF genes related to Sclerotinia sclerotiorum resistance in Brassica napus

Screening and functional analysis of GSTF genes related to S...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Tian Shifu Gao Xiuqin He Dou Wang Airong State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops College of Plant Protection Fujian Agriculture and Forestry University

*** is a significant oilseed crop in *** stem spot caused by *** is the most devastating disease in *** present,there are no immune or high-resistance varieties in *** is a difficult problem to urgendy solve the rapeseed germplasm resource that is resistant to *** is unknown whether rapeseed Glutathione-s-transferase（GST） is involved in the immune response to *** study was based on the results of previous studies of GST gene family resistance in Arabidopsis thaliana,using RNAseq,RT-PCR techonology to screen pathogen or hormone-induced up-regulated genes from *** and obtain seven *** to the degree of induced expression,BnaA02g20640D,BnaC03g30870D and BnaA03g14140D were studied *** three genes belong to the GSTF family,we cloned BnaA03g14140D,BnaA02g20640D,BnaC03g30870D into different overexpression vectors（pEG103 or pCAMBIA3301） and transformed into the Nicotiana benthamiana by Agrobacterium-mediated injection of leaf tissue ***,the target genes were stably expressed in *** leaves and *** inoculated in the injection area of the *** inoculation results showed that the average lesion size of wild type was 160.6 mm,while the average lesion size of 35 S::BnaA03g14140D-GFP,35 S::BnaA02g20640D-GFP and 35 S::BnaC03g30870D-GFP were smaller than that of wild *** were 83.4 mm,144.9 mm,and104.7 mm,*** results preliminarily demonstrated that these four genes are likely to be closely related to the resistance to disease,pending further study.

关键词： Hormone induction Brassica napus Sclerotinia sclerotiorum Glutathione-s-transferase

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Research progress on Litchi disease and their prevention and control

Research progress on Litchi disease and their prevention and...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：王松 Mao Ziling 单体江 Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitectureSouth China Agricultural University

Litchi chinensis Sonn was one of the most important economic fruit trees in South *** the large-scale planting and the growing number of cultivated varieties,litchi diseases were becoming more and more *** 10 litchi diseases were reported at home and abroad,namely Litchi downy blight,Acidosis,Anthracnose,Ulcer disease,Leaf spot,Powdery mildew,Dark mildew,Broomstick disease,Felt disease and Algae spot disease,*** downy blight,Acidosis,Broomstick disease,and Felt disease caused by Aceria litchi(Keifer) were the main diseases,and the damages were also the most *** diseases were mainly fungal *** them,Anthracnose,Leaf spot and Dark mildew mainly damaged to the leaves;Ulcer disease mainly caused damages on the trunk;Powdery mildew can cause damages on the leaves and *** disease was a virus disease caused by LWBV and the transmission vectors were Comegenapsylla sinica(Linnaeus) and Tessaratoma papillosa *** spot disease mainly had damages on the leaves and always occurred in the litchi garden with high humidity and poor *** prevention and control were still the most important means for the diseases of ***,the problems caused by chemical pesticides also became increasingly prominent,such as pesticide residues,food safety,reduced natural enemies,environmental pollution and increasing drug *** prevention and control of litchi diseases was a comprehensive and persistent *** chemical control no longer met the needs of today's society and *** litchi diseases,prevention was the prerequisite,mainly depended on agricultural prevention,physical prevention and biological control,supplemented by chemical *** was necessary for the growers to master the law of occurrence and development of diseases,to explore three-dimensional and ecological cultivation,and to use various control *** addition,the global climate was complex and changeab

关键词： Litchi Disease Prevention and control Research progress

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Rapid mapping of a stripe rust resistance gene in wheat cultivar Zhongliang 31 using BSA combined with high-throughput SNP genotyping arrays

Rapid mapping of a stripe rust resistance gene in wheat cult...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Liu Huan Su Wenwen Chao Kaixiang Li Qian Yue Weiyun Wang Baotong Li Qiang State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University Tianshui Institute of Agricultural Sciences

Stripe rust,caused by Puccinia striiformis ***（Pst）,is one of the most important diseases of wheat（Triticum aestivum L.） and causes substantial yield losses in many wheat-growing regions of the *** and utilization of new resistance genes is the most effective way for achieving durable disease *** 31,a common wheat highly resistant to Chinese predominant Pst races both in the seedling and adult-plant stages,has been grown widely in Longnan region in Gansu Province where is the hotspot for stripe rust *** analysis of F and F populations indicated that the stripe rust resistance of Zhongliang 31 is controlled by a single dominant gene,temporarily designated as *** segregant analysis（BSA） combined with wheat 660 K single nucleotide polymorphism（SNP） array showed 2050 of 9118 polymorphic SNPs located on chromosome 2 B.141 kompetitive allele specific PCR（KASP） selected from the 2050 SNPs and 165SSR markers on chromosome 2 B were used to map *** analysis indicated that 49 markers were linked to Yrzl31 and the genetic distances of two closest flanking markers KASP AX-111650565 and AX-110196738 were2.8 cM and 4.1 cM,***,Yrzl31 was located on wheat chromosome 2 *** chromosome location,resistance and molecular tests suggested that Yrzl31 may be a novel *** this gene is present at low frequency in the current wheat germplasms in China,it can be pyramided with other effective stripe rust resistance genes using marker-assisted selection（MAS）.

关键词： Puccinia striiformis f.sp.tritici Resistance gene Mapping BSA SNP

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Isolation and identification of an antagonistic actinomycete from rhizosphere soil of tobacco

Isolation and identification of an antagonistic actinomycete...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：陈奇园 Bian Chuanhong Chen Qianqian 康业斌 College of Forestry Henan University of Science and Technologe

From May to September 2014,the antagonistic actinomyces strain LA5 with a strong inhibition activity to Phytophthora parasitica was isolated from rhizosphere soil of tobacco in Luoning county of Luoyang,Henan *** strain were identified by using gram staining,observing the morphological characteristics by using oil immersion lens and cultural characteristic,determining physiological and biochemical characteristic,and analyzing the sequences of 16S *** research achievements as follows:（1） The Gram stain test showed that the strain LA5 is *** and straight spore chains were observed,rod-shaped spores and sporangia were found,and pear-shaped sporangia developed on the substrate mycelia under the light microscopy.（2） On the Gause No.1 medium,the strain LA5 produces ivory yellow substrate mycelia and snowwhite aerial mycelia,no soluble pigment.（3） The strain LA5 is positive for hydrolysis of starch,coagulation and peptonization of milk,liquefaction of gelatin,production of HS and negative for decomposition of *** show that this strain can produce amylase,protease,which does not produce cellulase.（4） The almost-complete 16S rDNA gene sequence of strain LA5 was determined and deposited in the GenBank databases as *** to Streptornyces lincolnensis were greater than 98%.Phylogenetic trees were constructed with the neighbour-joining algorithm using Molecular Evolutionary Genetics Analysis（MEGA） software version *** data suggest that strain LA5 should be Streptornyces lincolnensis.

关键词： Antagonistic actinomycetes Morphological identification Physiological and biochemical characteristics Molecular identification Rhizosphere soil Tobacco

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Rab GTPases are important for retromer complex-mediated trafficking in Fusarium graminearum

Rab GTPases are important for retromer complex-mediated traf...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Qiu Han Wu Huiming Fang Wenqin Qin Wusa Zhang Jing Lu Guodong Zheng Wenhui State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University

The retromer complex,composed of a cargo-selective complex(Vps35-Vps29-Vps26)and a Vps5-Vps17 dimer,mediates the sorting and retrograde transport of cargo proteins from the endosomes to trans-Golgi network in eukaryotic ***35-Vps29-Vps26 trimer has been shown to interact with cargo proteins while Vps5-Vps17 dimer binds the endosomal membranes for proper anchorage of the *** proteins belong to the Ras superfamily of small GTPases and regulate many trafficking events including vesicle formation,budding,transport,tethering,docking and fusion with target *** both of them play similar roles in endosomal trafficking,we investigated the potential relationship between the retromer complex(Vps35,Vps17) and the Rab proteins(Rab2,Rab4,Rab5,Rab7,Rab7,Rab8,Rabx,yptA) in Fusarium *** expressed Vps35-GFP and Vps17-GFP in each RAB gene deletion mutant and then observed localization in each transformant using highresolution laser confocal *** found that the Vps35-GFP and Vps17-GFP are both mislocalized and diffused in the cytoplasm of ArabS mutant as compared with their punctate nature within the endosomes of the wild ***,the Vps35-GFP and Vps17-GFP florescent dots are highly accumulated in the sub-tip of growing hyphae in Δrab7 *** addition,the Vps35-GFP and Vps17-GFP expressed in the Δrab8 mutant showed larger punctation size than the wild ***,we conclude that the Rab proteins Rab5,Rab7 and Rab8 play important roles in regulating retromer-mediated trafficking in ***.

关键词： Retromer Rab GTPases Fusarium graminearum Vesicle trafficking

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N gene enhances resistance to Chilli veinal mottle virus and hypersensitivity to salt stress in Nicotiana tobacum

N gene enhances resistance to Chilli veinal mottle virus and...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Yang Ting Lv Rui Xu Zhenpeng Zhu Lisha Peng Qiding Qiu Long Lin Honghui Xi Dehui Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University

Plants use multiple mechanisms to fight against pathogen *** resistance(R) gene which specifically mediate plant defense is one of the major mechanisms,while recent studies have shown that R genes have broad spectrum effects in response to various stresses.N gene is the resistance gene specifically resistant to Tobacco mosaic virus(TMV).However,the role of N gene in abiotic stress and other viral response remains *** veinal mottle virus(ChiVMV),a member of Potyvirus genus which contain the largest group of known plant viruses and comprise many agriculturally and economically important *** this study,we investigated the mechanisms by which N regulated plant defense responses under ChiVMV infection and salt ***,we monitored the physiological and molecular changes of tobacco plants under virus *** results showed that when tobacco and tobacco plants were challenged with ChiVMV,tobacco plants displayed more susceptibility at 5 days post infection(dpi),while tobacco plants exhibited more susceptibility at 20 *** accumulation of ROS and NtHIN1 expression were higher in tobacco than tobacco plants at 5 dpi,and the defense gene NtPR1,the activities of antioxidant enzymes and salicylic acid(SA) content were increased in tobaccoplants compared with tobacco *** was suggested that N gene induced hypersensitive response(HR) and enhanced the systemic resistance of plants in response to ChiVMV via a SA-dependent signaling pathway,which could be supported by some studies that systemic necrosis shared HR attributes.N gene induced HR and enhanced the systemic resistance of plants in response to ChiVMV.N gene could also be induced significandy by salt ***,tobacco plants showed hypersensitivity toward increased salt stress,and this hypersensitivity was dependent on abscisic acid and jasmonic acid but not *** together,our results indicated that N gene appeared to important for tobacco plants in response to ChiVMV infect

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辽宁省西瓜细菌性果斑病病原鉴定及其ITS序列分析

辽宁省西瓜细菌性果斑病病原鉴定及其ITS序列分析

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者：于海博夏博夏子豪安梦楠吴元华沈阳农业大学

西瓜细菌性果斑病(watermelon bacterial fruit blotch)是西瓜上的重要病害。2017年在辽宁省锦州市右卫镇大面积暴发,发病面积达500余亩,发病率高达80%,几乎近绝产。病害症状为叶片出现不规则褐色坏死病斑,偶尔叶斑上溢出白色菌脓;果实... 详细信息

西瓜细菌性果斑病(watermelon bacterial fruit blotch)是西瓜上的重要病害。2017年在辽宁省锦州市右卫镇大面积暴发,发病面积达500余亩,发病率高达80%,几乎近绝产。病害症状为叶片出现不规则褐色坏死病斑,偶尔叶斑上溢出白色菌脓;果实上有明显水渍状暗绿色大斑,后期果皮龟裂,表面溢出黏稠、透明的琥珀色菌脓。从田间发病西瓜植株上分离获得菌株JZ17,致病性测定表现出与田间病害相同的症状,且从接种发病植株重新分离到完全相同的细菌。该供试菌株在KB培养基上呈现乳白色圆形菌落,光滑,不透明,无黏性,中间稍突起,边缘整齐,不产荧光,菌落大小为1～2mm,能在41℃下生长,不能在4℃下生长。光学显微镜观察表明,病原菌菌体短杆状,大小为(0.2～0.8)μm×(1.0～5.0)μm,革兰氏阴性,严格好氧,极生单根鞭毛。采用西瓜噬酸菌(Aac)检测试剂对病原菌进行DAS-ELISA血清学鉴定,结果表明供试菌株的A405值分别为3.784、3.767和3.775,远大于2倍的阴性对照A405值(0.144);利用特异性引物WFB1和WFB2对菌株16SrDNA基因序列进行扩增,获得了360bp的DNA片段,菌株JZ17与4cidovorax citrulli各菌株16SrRNA的同源性均高达99%。因此将此致病菌株JZ17鉴定为西瓜嗜酸菌(Acidovorax citrulli)。利用pilL基因设计的特异性引物PL1、PL2扩增目的片段,结果表明供试菌株JZ17和对照菌株AAC00-1(亚群Ⅱ)可扩增出大小为332bp的DNA片段,而对照菌株pslbtw8(亚群Ⅰ)不能扩增出条带,因此将菌株JZ17鉴定为亚群Ⅱ型菌株。根据噬酸菌属ITS序列比对,设计特异性引物YH1/YH2,对供试菌株JZ17的16SrDNA部分片段、16S-23SrDNA全部片段及23SrDNA部分片段进行扩增及序列测定分析,获得了850bp的DNA目的片段,并将序列上传到NCBI(序列号为MG655621),采用MEGA 6.05构建系统发育进化树,结果表明菌株JZ17只与西瓜噬酸菌不同菌株聚为一类,自展支持率达100%,具有较近的亲缘关系。

关键词：西瓜细菌性果斑病病原菌鉴定西瓜嗜酸菌亚群鉴定

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