Banana bunchy top virus(BBTV) is a circular sinG1 e-stranded DNA virus with *** infection mechanism and pathogenesis is remaining *** this study,the BBTV B2 isolate from the Southeast-Asia group was used for PCR *** C...
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Banana bunchy top virus(BBTV) is a circular sinG1 e-stranded DNA virus with *** infection mechanism and pathogenesis is remaining *** this study,the BBTV B2 isolate from the Southeast-Asia group was used for PCR *** CP gene(528 bp) and NSP gene(479 bp) were subsequently constructed into the plant expression vector pCAMBIA1300(GV1300;GFP),*** recombinant plasmids were transformed into Nicotiana benthamiana leaves by Agrobacterium tumefaciens GV3101 strain and subcellular localization of CP and NSP proteins in *** leaves were ***,total DNAs of four BBTV infected banana leaves of different cities in China were conducted to analyze the relative DNA amount of CP and NSP genes by the quantitative PCR(qPCR) *** results showed that CP protein was located in the nucleus,while NSP protein was distributed in the nucleus and *** analysis indicated that the DNA content of CP and NSP genes are varieties of different BBTV *** DNA content of CP was very high in B2(Haikou,China) and FS1(Chengmai,China),and was medium in FZ1(Fuzhou,China),but the lowest in YC5(Sanya,China).For NSP gene,the DNA content of B2,FS1 and FZ1 was at least 50 000 times higher than of *** this study,the subcellular localization of BBTV CP and NSP proteins in tobacco plant leaves was clarified,and the relative DNA content of CP and NSP genes of different BBTV isolates was *** study provides an important basis for further clarifying the infection mechanism and pathogenesis of BBTV CP and NSP genes.
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