Huanglongbing(HLB) is the most destructive disease of citrus *** disease is caused by Candidatus Iiberibacter spp.,which vectored by the psyllids Diaphorina citri Kuwayama and Trioza *** proteins are important in bact...
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Huanglongbing(HLB) is the most destructive disease of citrus *** disease is caused by Candidatus Iiberibacter spp.,which vectored by the psyllids Diaphorina citri Kuwayama and Trioza *** proteins are important in bacterial pathogenesis and structure *** of them express at high *** obtain at least one high-level expression of secretory protein genes(SPGs),ten candidated SPGs were chosen from the Candidatus Iiberibacter asiaticus by bioinformatic *** SPGs were tested by PCR,qPCR and RT-qPCR method,*** result showed that two SPGs,408 and pap,are highly *** 408 and pap genes were further constructed into the plant expression vector pCAMBIA 1300(GV1300:GFP) and expressed in tobacco leaf epidermal cells for subcellular localization *** transient expression results indicated that 408 protein is located in the nucleus and cytoplasm of tobacco leaf ***,pap protein was located in the radially arranged micro-fibrils of tobacco guard cells that may relate to help pathogen invade into plant *** this study,408 and pap secretory protein genes of Candidatus Iiberibacter asiaticus were obtained and the subcellular localization of these two genes in tobacco leaves was *** research laies an important foundation for the preparation of the antiserum against the Candidatus Iiberibacter asiaticus and the early detection and prevention of citrus HLB.
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