False smut of rice,caused by Ustilaginoidea virens,has been reported in most ricegrowing areas of China and emerged as one of the major rice diseases *** pathogen is predicted to secrete a large array of effector cand...
详细信息
False smut of rice,caused by Ustilaginoidea virens,has been reported in most ricegrowing areas of China and emerged as one of the major rice diseases *** pathogen is predicted to secrete a large array of effector candidates,which play important roles in *** ***,the specific virulence function has not been reported for any individual *** *** this study,we confirmed that the small cysteine-rich effector candidate SCREl in *** expressed SCRE1 in *** inhibited hypersensitive responses induced by BAX,INF1 and Burkholderia glumae *** expression of SCREl in rice suppresses the oxidative burst and PR gene expression triggered by MAMP,and also enhances disease *** further demonstrated that the effector candidate SCREl entered the plant cell during the infection,indicating that the secreted protein is a bona fide *** interactor of SCRE1 has been identified and to be *** study aims to uncover a novel mechanism for SCRE1 to suppress plant *** findings provided information and materials for understanding molecular mechanisms underlying virulence and pathogenesis of ***.
NLP(Necrosis and ethylene inducing peptide like protein)是微生物中广泛存在的一类可以引起双子叶植物细胞死亡和诱导乙烯生成的蛋白。目前对于NLP引起双子叶植物细胞死亡的机理还了解不多。本研究借助拟南芥这一模式植物来解析NLP...
详细信息
NLP(Necrosis and ethylene inducing peptide like protein)是微生物中广泛存在的一类可以引起双子叶植物细胞死亡和诱导乙烯生成的蛋白。目前对于NLP引起双子叶植物细胞死亡的机理还了解不多。本研究借助拟南芥这一模式植物来解析NLP引起细胞死亡的遗传机制。在前期工作基础上,我们发现NLP引起的拟南芥细胞死亡反应是一种数量性状,并且在Zu-1 XCol-0重组自交系中发现了两个贡献表型变异的位点QZ1.1和QZ4.1。其中QZ4.1位于SNPCZ427和SNPCZ103之间,对表型贡献率达到18%。为了解析QZ4.1调控NLP激发细胞死亡的分子机制,我们对该位点进行了精细定位。首先我们在F2群体中挑选QZ4.1区间纯合植株为母本,构建回交群体。通过筛选42个回交二代群体发现QZ4.1位点质量化群体ZC46Z1。该群体在NLP处理后,纯合Col-0基因型和杂合植株死亡率显著高于纯合Zu-1基因型植株,从而确定在QZ4.1位点Col-0基因型决定了植物对NLP的敏感性。通过筛选ZC46Z1群体约8 000个植株,共计获得768个在SNPCZ427和SNPCZ103之间发生遗传交换的重组单株。通过对重组单株进行表型分析,进一步将候选区间缩小到约100kb的区间。该区间内含有18个基因,编码多个TIR-NB-LRR蛋白,跨膜蛋白及激酶等潜在的与受体识别及信号转导相关的蛋白质。我们推测QZ4.1可能是一个TIR-NBS-LRR蛋白,它可能直接识别NLP并将信号传递到下游,从而调控细胞死亡。目前正在对这些候选基因进行功能互补验证,其结果将为深入认识NLP激发双子叶植物细胞死亡的分子机制奠定基础。
暂无评论