检索结果-内蒙古大学图书馆

您好，读者！请登录

内蒙古大学图书馆

首页
概况
党建
资源
服务
科研支持
- 论文收录引用证明
- 科技查新
知识产权
档案馆
帮助

咨询与建议

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

您的常用邮箱：*

您的手机号码：*

问题描述：

当前已输入0个字，您还可以输入200个字

全部搜索
期刊论文
图书
学位论文
标准
纸本馆藏
外文资源发现
数据库导航
超星发现

高级检索

时间限定

出版年份：

文献类型

图书期刊文献学位论文多媒体

馆藏选择

电子馆藏纸本馆藏

核心期刊

全部期刊 SCI 收录期刊 SSCI 收录期刊 EI 收录期刊 CSCD 收录期刊 CSSCI 收录期刊

语言

中文英文

文献类型

期刊文献图书学位论文标准纸本馆藏

帮助

文字说明：

T=题名（书名、题名），A=作者（责任者），K=主题词，P=出版物名称，PU=出版社名称，O=机构（作者单位、学位授予单位、专利申请人），L=中图分类号，C=学科分类号，U=全部字段，Y=年（出版发行年、学位年度、标准发布年）

检索规则说明：

AND代表“并且”；OR代表“或者”；NOT代表“不包含”；(注意必须大写,运算符两边需空一格)

检索范例：

范例一：(K=图书馆学 OR K=情报学) AND A=范并思 AND Y=1982-2016
范例二：P=计算机应用与软件 AND (U=C++ OR U=Basic) NOT K=Visual AND Y=2011-2016

分类表

所选分类

>> <<

限定检索结果

文献类型

504 篇 会议

馆藏范围

504 篇 电子文献
0 种 纸本馆藏

日期分布

学科分类号

501 篇 农学
- 484 篇 植物保护
- 16 篇 作物学
- 2 篇 园艺学
9 篇 理学
- 5 篇 生态学
- 4 篇 生物学

主题

12 篇 致病性
10 篇 禾谷镰刀菌
9 篇 pathogenicity
9 篇 水稻
9 篇 鉴定
8 篇 生物防治
8 篇 稻瘟菌
8 篇 稻瘟病
7 篇 phytophthora inf...
7 篇 稻瘟病菌
6 篇 小麦
6 篇 基因功能
6 篇 原核表达
6 篇 杀菌剂
6 篇 多样性
6 篇 sclerotinia scle...
5 篇 抗病性
5 篇 致病力
5 篇 效应蛋白
5 篇 生防菌

机构

49 篇 中国农业大学
15 篇 海南大学
15 篇 华南农业大学
15 篇 福建农林大学
14 篇 西南大学
12 篇 江苏省农业科学院...
12 篇 海南大学植物保护...
12 篇 广西大学
12 篇 华中农业大学
10 篇 河北农业大学
10 篇 河南农业大学
9 篇 fujian key labor...
9 篇 沈阳农业大学
8 篇 安徽农业大学
8 篇 青岛农业大学
8 篇 长江大学
8 篇 内蒙古大学
7 篇 黑龙江八一农垦大...
7 篇 state key labora...
7 篇 宁夏大学

作者

24 篇 缪卫国
18 篇 刘文波
12 篇 林春花
9 篇 韩成贵
9 篇 王颖
9 篇 孙文献
8 篇 哈达
8 篇 洪霓
8 篇 王国平
8 篇 zhan jiasui
8 篇 姜道宏
8 篇 谢甲涛
7 篇 刘大群
7 篇 张宗英
7 篇 刘永锋
7 篇 wang zonghua
7 篇 李保华
7 篇 程家森
7 篇 陈保善
6 篇 靳鹏飞

语言

367 篇 中文
137 篇 英文

检索条件"任意字段=中国植物病理学会第十一届全国会员代表大会暨2018年学..."

共 504 条记录，以下是401-410 订阅

全选清除本页清除全部题录导出标记到"检索档案"

详细简洁

排序：

SsFDH1,a formaldehyde dehydrogenase,regulates the sclerotial development and pathogenicity in Sclerotinia sclerotiorum

SsFDH1,a formaldehyde dehydrogenase,regulates the sclerotial...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Zhu Genglin Li Jingtao Lv Xingming Zhang Xianghui Liu Jinliang Zhang Yanhua Pan Hongyu College of Plant Sciences Jilin University

Sclerotinia sclerotiorum(Lib.) de Bary is notorious phytopathogenic fungus with broad host ***-dependent formaldehyde dehydrogenases(GD-FDHs) represent a ubiquitous class of enzymes in both prokaryotes and eukaryotes,but the functions of FDHs in necrotrophic plant pathogen Sclerotinia sclerotiorum have not been *** this study,we functionally characterized a GD-FDH orthologous protein SsFDH1(SS1G0135)in ***1 was firstly identified as an interacting protein of our previously characterized GATA transcription factor SsNsdl,which regulates asexual-sexual development and appressoria formation in *** to its predicted biochemical function as one of the FDHs in formaldehyde detoxification,SsFDH1 deletion mutants(△SsFDH1-KOs) were lethal when grown in formaldehyde-containing medium and showed sensitivity to exogenous nitrosative stress as ***,△SsFDH1-KOs showed altered sclerotial development,reduced production of compound appressoria,as well as increased sensitivity to different osmotic and oxidative ***,the △SsFDH1-KOs exhibited attenuated virulence on both healthy and wounded common bean *** with the previously generated △SsNsd-KOs,△SsFDH1-KOs produced smaller-sized sclerotium,but showed similar attenuated ***,we found that the expression levels of both SsNsd1 and SsFDH1 displayed spatial and temporal synchronization as revealed by quantitative real-time PCR(qRT-PCR) analysis in different developmental *** together,our results suggested that SsFDH1,as a SsNsd1-interating protein,might function jointly with SsNsd1 in vegetative development and pathogenicity in ***.

关键词： Sclerotinia scierotiorum SsFDH1 Vegetative development Appressorium Pathogenicity SsNsd1

来源：评论

学校读者我要写书评

暂无评论

Different strategies to properly manage the citrus huanglongbing in China

Different strategies to properly manage the citrus huanglong...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Shahzad Munir Li Yongmei He Pengfei Wu Yixin He Pengjie Cui Wenyan 何月秋 Faculty of Plant Protection Yunnan Agricultural University

Huanglongbing(HLB) also known as citrus greening is one of the serious diseases spread all over citrus growing regions of the *** causal agent is Candidatus Liberibacter asiaticus,a phytopathogenic bacterium that can infect all Citrus *** causal agents of HLB have been putatively identified,and their transmission pathways and worldwide population structure have been extensively ***,very little isknown about the epidemiologic relationships of ***,which has limited the scope of HLB research and especially the development of control ***-affected plants produce damaged fruits and die within several *** candidate compounds show promise in eliminating or suppressing disease symptoms;however,others need to be evaluated in the form of antibiotics with less ***,thermotherapy can be useful for combating *** application of epibrassinolide reduces the causal agent of HLB and might provide a useful tool within an integrated management *** in oil(W/O) nanoemulsion formulation may provide a useful model for the effective delivery of chemical compounds into citrus phloem via a foliar spray for controlling HLB citrus *** production of transgenic plants with modified volatile profiles that are more tolerant to citrus greening disease can be helpful in reducing the disease ***,the potential impact on HLB should be conducted for discovering novel antibacterial *** research is needed to provide an eco-friendly control strategy for HLB in the form of ***,unfortunately,no study has reported the successful control of the disease by microbial control agents *** HLBresistant citrus varieties are being developed,it will presumably take many years before they are ***,alternative measures are being used to reduce the incidence of this disease or HLB pathogens in the *** far,there are no curative methods to control th

关键词： HLB Different strategies to properly manage the citrus huanglongbing in China

来源：评论

学校读者我要写书评

暂无评论

解淀粉芽胞杆菌B9601-Y2对玉米防病促生研究

解淀粉芽胞杆菌B9601-Y2对玉米防病促生研究

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：崔文艳何朋杰何鹏飞吴毅歆 Shahzad Munir 何月秋云南农业大学植物保护学院微生物菌种筛选与应用国家地方联合工程研究中心云南农业大学农学与生物技术学院

玉米是我国主要粮食作物,病害是影响玉米产量和品质的主要因素之一。小斑病和茎基腐病是我国危害大、分布广且逐年加重的主要玉米病害。目前主要依靠化学农药,但长期大量使用化学药剂会带来一系列环境问题,因此,采用新型的防治策略迫在...

玉米是我国主要粮食作物,病害是影响玉米产量和品质的主要因素之一。小斑病和茎基腐病是我国危害大、分布广且逐年加重的主要玉米病害。目前主要依靠化学农药,但长期大量使用化学药剂会带来一系列环境问题,因此,采用新型的防治策略迫在眉睫。生物防治因具有环境友好且可持续等特点,已成为当今研究的热点领域。B9601-Y2(Y2)是本实验室分离的一株解淀粉芽胞杆菌植生亚种(Bacillus amyloliquefaciens ***)菌株,具有促进种子萌发和植物的插条生根、提高作物出苗率、加快植物生长等特点。Y2可以分泌伊枯草菌素、丰齐素和聚酮类抗生素,能够拮抗纹枯病菌、炭疽病菌等多种常见病原真菌和细菌病害。本文通过研究Y2对玉米小斑病菌和茎基腐病菌的室内平板拮抗效果以及在玉米盆栽实验中的防病促生表现,为Y2菌株对玉米的促生效果及相关机制研究和为将其生产成控病、减肥增效的玉米微生物肥料奠定了基础。平板对峙培养结果表明,Y2发酵液、无菌滤液及菌体悬液对玉米小斑病菌和茎基腐病原菌均有拮抗作用;固体培养和液体培养结果表明,Y2发酵液、无菌滤液及菌体悬液均能够不同程度地抑制菌丝生长和孢子萌发,显微结构观察显示Y2发酵液能够裂解菌丝和孢子。盆栽防病实验结果显示,3种处理对玉米小斑病防效60%左右,对茎腐病防效50%左右,防效依次为Y2发酵液>菌体悬液>无菌滤液,且使用Y2的防治效果好于治疗效果。单独使用Y2发酵液、无菌滤液及菌体悬液处理的苗根长、植株型高、鲜重和干重均显著高于清水和病原菌对照20%以上,喷施或灌溉接种病原菌的玉米苗生长量显著高于单独接种病原菌处理,有效消除了病原菌对植株生长的影响;此外,从植株生长情况来看,先喷施或灌溉生防菌Y2后接种病原菌的植株生长量显著高于先接种病原菌后喷施或灌溉生防菌Y2的植株。因此,生防菌Y2具有用于防治玉米病害和具有根肥及叶面肥的潜力。

关键词：

来源：评论

学校读者我要写书评

暂无评论

In vitro and in vivo effectiveness of phenolic compounds for the control of postharvest gray mold of table grapes

In vitro and in vivo effectiveness of phenolic compounds for...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Xu Dandan Deng Yizhen Xi Pinggen Wang Qi Jiang Zide Gao Lingwang College of Plant Protection China Agricultural University Department of Plant Pathology Guangdong Province Key Laboratory of Microbial Signals and Disease ControlSouth China Agricultural University

Botrytis cinerea is the pathogen of gray mold disease leads to huge postharvest losses and is considered as the main postharvest decay of table grapes（Vitis vinifera）.In consideration of the hazardous effect of SO application on human health and environment,alternatives to SO are urgently *** the natural antimicrobial metabolites,phenolic compounds were reported to be effective in the inhibition of phytopathogenic fungi,including the postharvest decay ***,comprehensive study on the biological activity of phenolic compounds and their application on controlling postharvest gray mold of table grapes is *** this study,the antifungal effect of 18 natural or synthetic phenolic compounds purchased from commercial suppliers,including simple phenolic,phenolic acids,stilbenes and flavonoids,were determined on four gray mold strains by the agar dilution ***,seven phenolic compounds（naringenin,coumarin,resveratrol,ferulic acid,catechol,piceatannol and pterostilbene） were effective on inhibiting *** growth and were selected to test their activity on conidial germination as well as in vivo application on grape *** showed the highest antifungal activity and greatly reduced the growth of the mycelia,caused hyphae deformation,suppressed conidial germination of ***,and completely inhibited the germination of conidia at the concentration of 50 mg/***,treatment of grape berries with pterostilbene and piceatannol significantly reduced the disease incidence and *** results demonstrate the antifungal activity of phenolic compounds and highlight the great potential of natural compounds to be used as alternative strategy to traditional fungicides in the control of postharvest gray mold of table grapes.

关键词： Antifungal activity Piceatannol Postharvest decay Pterostilbene

来源：评论

学校读者我要写书评

暂无评论

Transcriptome profiling of maize reveals resistance gene changes in response to DZSY21 induction

Transcriptome profiling of maize reveals resistance gene cha...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Wang Qi Chen Xiaojie Gu Shuangyue Zhang Xinyue Hang Tianlu Ding Ting College of Plant Protection Anhui Agricultural UniversityAnhui Key Laboratory of Crop Biology

Endophytic bacteria DZSY21 can stably colonize and enhance the defense response in ***,this study was designed to predict the molecular mechanism of maize defense response induced by endophytic bacteria DZSY21 at transcription *** transcriptome sequencing technology were used to analyze the differential expression pattern of genes in maize leaves after treatment with DZSY21 for different *** in the expression level of Fold change ≥2 and FDR<0.01 were recognized as differentially *** sample inoculated DZSY21 for Oh was set as *** this criterion was applied,a total of 2 413 genes showed significantly altered expression levels in response to strain DZSY21 for 12 *** these,1 278 were up-regulated and 1 135 were *** inoculating with DZSY21 for 24 h,737 genes including 538 up-regulated DGEs and 199 down-regulated DGEs were differentially *** to the results of functional annotation,218 differentially expressed genes(DEGs) involved in 26 resistance pathways were obtained after inoculating for 12 h,which including Bifunctional inhibitor/lipid-transfer protein,MATE efflux family protein and LysM domain receptor-like *** were 71 DEGs were involved in 30 resistance pathways after inoculated 24 h,including Isoflavone reductase,Probable cellulose synthase,Phenylalanine ammonia-lyase,L-ascorbate peroxidase,Probable transcription factor GLK and ACC oxidase,The above results showed that the endophytic bacteria DZSY21 was introduced into corn and could regulate the expression of resistancerelated genes in maize *** laid the foundation for further searching for resistance genes and their function identification.

关键词： DZSY21 Maize Resistance gene Transcriptome

来源：评论

学校读者我要写书评

暂无评论

Secondary metabolites and antimicrobial activities of Chaetomium

Secondary metabolites and antimicrobial activities of Chaeto...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：欧阳锦逵 Wu Chunyin Mao Ziling 单体江 Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitectureSouth China Agricultural University

Chaetomium were the most abundant and significant fungal species of Ascomycota,belonging to Ascomycota,Pyrenomycetes,Sordariomycetidae,Sordariales and *** globosum was the model species and more than 400 taxa were described since the establishment of this *** were important microbial resources that can produce polytype secondary *** date,more than 200 compounds were isolated and reported,including Azaphilones(36),Chaetoglobosins(34),Diketopiperazines(21),Depsidones(14),Orsellides(10),Steroids(8),Chromones(5),Tetramic acids(4),Isoquinolines(3),Terpenoids(3),Pyrones(2) and other types *** them,more than 160 were new compounds,which accounting for up to 80%.However,there were few reports about antimicrobial activity of secondary metabolites isolated from *** now,only seven compounds showed antibacterial activities and six compounds displayed antifungal *** displayed the best antibacterial activities and the MIC values of Chaetocin and Chetracins A-C against Staphylococcus aureus were 0.025-0.39μg/*** FR207944,which was a rare triterpenoid glycoside,showed the best antifungal activities on Aspergillus fumigatus and the MIC value was 0.039μg/*** study indicated that the strain of Chaetomium was worthy for further *** article would provide important theoretical basis for the development of novel biological pesticides and the comprehensive development and utilization of Chaetomium microbial resources.

关键词： Chaetomium Secondary metabolites Antimicrobial activities

来源：评论

学校读者我要写书评

暂无评论

A reverse genetics platform for tobacco bushy top disease complex

A reverse genetics platform for tobacco bushy top disease co...

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Zhao Xingneng Zhang Lifang Xu Ping Li Yanqiong Xia Zhenyuan Chen Hairu Mo Xiaohan Yunnan Academy of Tobacco Agricultural Sciences College of Plant Protection Yunnan Agricultural University

Tobacco bushy top disease(TBTD) is the only plant disease caused by a luteovirus/umbravirus complex in China and its causal agent,TBTD complex,is one of the most complicated plant virus *** coat proteins of the virions of TBTD complex are encoded by tobacco vein distorting virus(TVDV) only,while there are five distinct viral RNA components being encapsidated in,namely,TVDV,tobacco bushy top virus(TBTV),tobacco vein distorting virus-associated RNA(TVDVaRNA),TBTV satellite RNA,and an unidentified viral ***-mediated infectious clones of TVDV,TBTV,TVDVaRNA and TBTV satellite RNA were constructed,serving as a reverse genetics platform to investigate the interactions among these viral components and their roles in the symptom developments and aphid *** preliminary application of this reverse genetics platform shed light on the interactions among different viral agents in the TBTD *** alone was able to replicate and move systematically and cause mild symptoms in planta(Nicotiana tabacum and ***).While the satellite RNA was dependent on TBTV for its replication and systematic movement,it boosted the symptom severity of the disease when coinfected with *** satellite RNA stimulated mildly the accumulation of TBTV genomic RNA in ***,while in ***,it downregulated the accumulation of TBTV genomic RNA slightly,indicating host specificity in the association of TBTV and its satellite *** alone could infect *** systemically without prominent *** plants developed intensive disease symptoms when coinfected with TVDV and TVDVaRNA,and the two viral components were able to move systemically in the plants,while TVDVaRNA alone could not infect *** *** results indicated that TVDV could facilitate the systemic movement of TVDVaRNA in planta,and TVDVaRNA helped TVDV in boosting the development of disease symptoms in *** this reverse genetics platform

关键词： A reverse genetics platform for tobacco bushy top disease complex

来源：评论

学校读者我要写书评

暂无评论

中国不同地区泡桐越冬休眠枝的植原体检测和组织培养研究

中国不同地区泡桐越冬休眠枝的植原体检测和组织培养研究

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：孔德治田国忠张文鑫于少帅王圣洁宋传生林彩丽中国林业科学研究院森林生态环境与保护研究所/国家林业局森林保护学重点实验室中国林业科学研究院热带林业研究所菏泽学院

泡桐(Paulownia spp.)是我国重要的速生用材树种,其自然分布范围较广,横跨亚热带与暖温带地区,涵盖了9个种、变种及丰富的遗传变异类型。而由植原体引起的泡桐丛枝病是危害泡桐的主要病害之一。前期的研究检测到不同地区发病泡桐体内的... 详细信息

泡桐(Paulownia spp.)是我国重要的速生用材树种,其自然分布范围较广,横跨亚热带与暖温带地区,涵盖了9个种、变种及丰富的遗传变异类型。而由植原体引起的泡桐丛枝病是危害泡桐的主要病害之一。前期的研究检测到不同地区发病泡桐体内的植原体株系之间存在着明显的分子变异,但尚不清楚这些遗传变异是否与病害发生与流行相关。本研究通过对不同株系、生境的发病泡桐越冬枝条外植体的组织培养获得不同遗传背景的植原体一寄主共生体,旨在阐明泡桐丛枝植原体不同株系间的致病性变异及其与寄主的互作关系。本研究分别从福建、四川、浙江、江苏、山西、陕西以及北京等地调查采集到不同品种的感病泡桐越冬休眠枝,进行室内水培试验,然后用水培萌发的嫩枝芽作为外植体进行组织培养。同时以新鲜叶片提取的总DNA为模板,进行植原体16S rDNA的直接PCR、巢式PCR和tuf-LAMP检测。结果显示:中国不同地区泡桐丛枝病发生程度差异很大,其中华北和黄淮海地区危害较重,南方地区危害较轻。各地采集的越冬存活病枝大多能水培萌芽,且部分病枝萌条即表现小叶和腋芽萌生等病状。对新鲜叶片样品提取的DNA采用常规的直接PCR检测,其产物均未检测到植原体的存在,而巢式PCR检测到部分样品植原体的繁殖;以本实验室建立的16SrⅠ-LAMP检测方法,同样检测到部分样品植原体的繁殖,进一步验证了巢式PCR检测结果。以休眠枝萌条和直接采自野外发病新鲜枝条作为外植体分别进行组织培养,发现来源于休眠枝萌条外植体的组培污染程度较轻,易于组培成功;根据外植体组培实验观察记录发现,升汞消毒时间控制在8～10min为宜。现已成功获得的福建、四川、江苏三省的泡桐带毒外植体组培苗中,部分组培苗已表现出植原体感染的典型丛枝、小叶症状。根据本研究初步推断我国不同株系、生境的泡桐丛枝植原体都能够在树枝部顺利越冬存活,次年随嫩枝萌芽而运转繁殖致病,而不必运转至地下根部越冬。为下一步对不同菌株组培苗的致病性的差异研究奠定基础。

关键词：泡桐丛枝病植原体 16S rDNA 休眠枝组织培养 LAMP检测

来源：评论

学校读者我要写书评

暂无评论

A model bacterial community of maize roots

A model bacterial community of maize roots

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Niu Ben Joseph Paulson Zheng Xiaoqi Wang Pengchao Yuan Zhibo Roberto Kolter Discipline of Forest Biological Engineering College of Life ScienceNortheast Forestry University Department of Microbiology and Immunobiology Harvard Medical School Department of Biostatistics and Computational Biology Dana-Farber Cancer Institute Department of Biostatistics Harvard T.H.Chan School of Public Health Department of Mathematics Shanghai Normal University

Plant-associated microbes are crucial for the health of their ***,the high complexity of plant microbiomes challenges detailed studies to define experimentally the mechanisms underlying the beneficial effects of such microbiota on plant hosts and the dynamics of community *** host-mediated selection,we obtained a greatly simplified synthetic bacterial community consisting of seven strains(Enterobacter cloacae,Stenotrophomonas maltophilia,Ochrobactrum pituitosum,Herbaspirillum frisingense,Pseudomonas putida,Curtobacterium pusillum and Chryseobacterium indologenes) representing three of the four most dominant phyla found in maize *** planta and in vitro,this model community inhibited the phytopathogenic fungus Fusarium verticillioides indicating a clear benefit to the *** utilizing a selective culture-dependent method to track the abundance of each strain we investigated the role that each plays in community assembly on roots of axenic maize *** the removal of *** led to the complete loss of the community and *** took *** suggests that *** plays the role of keystone species in this model ***,our synthetic seven-species community has the potential to serve as a useful system to dissect the beneficial effects of root microbiota on hosts and explore how bacterial interspecies interactions affect root microbiome assembly under laboratory conditions in future.

关键词： Maize Synthetic community Community assembly Biological control

来源：评论

学校读者我要写书评

暂无评论

侵染猕猴桃的一种Emaravirus属新病毒的鉴定

侵染猕猴桃的一种Emaravirus属新病毒的鉴定

引用

中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：王雁翔王国平洪霓华中农业大学植物科技学院/湖北省作物病害监测与安全控制重点实验室华中农业大学/农业微生物学国家重点实验室

欧洲山棒环斑病毒属(Emaravirus)是近年来成立的一个新属,已划分到布尼亚病毒目(Bunyavirales)无花果花叶病毒科(Fimoviridae)。Emaravirus属病毒基因组由4～8条负义单链RNA组成,每条RNA的互补链包含1个开放阅读框(ORF),RNA1-RNA4分别... 详细信息

欧洲山棒环斑病毒属(Emaravirus)是近年来成立的一个新属,已划分到布尼亚病毒目(Bunyavirales)无花果花叶病毒科(Fimoviridae)。Emaravirus属病毒基因组由4～8条负义单链RNA组成,每条RNA的互补链包含1个开放阅读框(ORF),RNA1-RNA4分别编码依赖于RNA的RNA聚合酶(RdRp)、糖蛋白(GP)、核衣壳蛋白(NP)和运动蛋白(MP),其他RNA链编码蛋白的功能未知。本研究室前期通过高通量测序技术鉴定到Emaravirus属的一种新病毒,并命名为猕猴桃褪绿环斑相关病毒(Actinidia chlorotic ringspot associated virus,AcCRaV)。本研究利用基于Emaravirus属病毒的RNA1保守区域设计的简并引物,对一株表现明显花叶症状的猕猴桃叶片样品进行RT-PCR分析,对扩增产物进行测序获得390bp的序列,BLASTX比对分析发现该序列与已报的无花果花叶病毒(FMV)的RdRp的部分氨基酸序列相似性为78.1%,初步推测该序列可能来源于一种Emaravirus属的病毒。采用基于Emaravirus属病毒末端序列设计的保守引物进行PCR扩增,电泳分析发现5条分子量大小为1 200～2 200bp的条带,对这些扩增产物分别进行了测序,获得了RNA2-5的序列,并根据已报道的Emaravirus属病毒RNA1的保守区域和已经获得的序列设计引物扩增出完整的RNA1序列。比对分析发现该病毒的RNA1～RNA6编码的蛋白列与Emaravirus属成员所编码的蛋白序列的相似性在10.7%～77.3%。其中,RNA3所编码的核衣壳蛋白序列与本研究室前期鉴定的AcCRaV编码的核衣壳蛋白序列相似性为37.4%。对RNA1-RNA4所编码的蛋白进行系统进化分析,发现该病毒与FMV和木豆不育花叶病毒2(PPSMV-2)聚为一个亚组,与AcCRaV的遗传距离较远。因此,认为该病毒是Emaravirus属一种新病毒,也是从猕猴桃上鉴定的第二种Emaravirus属病毒,根据猕猴桃症状表现,将该病毒暂命名为猕猴桃花叶相关病毒(Actinidiamosaic associated virus,AcMaV)。

关键词：猕猴桃新病毒 Emaravirus RNA 序列相似性核衣壳蛋白保守区域扩增产物

来源：评论

学校读者我要写书评

暂无评论

没有更多数据了...

全选清除本页清除全部题录导出标记到“检索档案”

共51页 << < 37 38 39 40 41 42 43 44 45 46 > >>

检索报告对象比较合并检索0

隐藏清空

合并搜索

回到顶部

执行限定条件

内容：

评分：

请选择保存的检索档案：

请选择收藏分类：

订阅名称：

通借通还

温馨提示：

图书名称：

借书校区：

取书校区：

手机号码：

邮箱地址：

一卡通帐号：

电话和邮箱必须正确填写，我们会与您联系确认。

联系人：

所在院系：

联系邮箱：

联系电话：

内蒙古自治区呼和浩特市赛罕区大学西街235号邮编: 010021

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：