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A short-chain acyl-CoA dehydrogenase is essential for mediating ER-β-oxidation,which is required for conidiogenesis and stress tolerance in the rice blast fungus

A short-chain acyl-CoA dehydrogenase is essential for mediat...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Sami Rukaiya Aliyu Lin Lili Waheed Abdul Chen Xiaomin Lin Yahong Frankine Jagero Otieno Ammarah Shabbir Wajjiha Batool Aron Osakina Tang Wei Wang Zonghua Justice Norvienyeku State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops the School of Life SciencesCollege of Plant ProtectionFujian Agriculture and Forestry University Fujian University Key Laboratory for Plant-Microbe Interaction the School of Life SciencesCollege of Plant ProtectionFujian Agriculture and Forestry University Institute of Oceanography Minjiang University

Short-chain acyl-CoA dehydrogenase(Scad) mediated β-oxidation serves as the fastest route for generating essential energies required to support the survival of organisms under stress or *** this study,we identified three putative SCAD genes in the genome of globally destructive rice blast pathogen Magnaporthe oryzae,named as MoSCAD1,MoSCAD1,and *** elucidate their function,we deployed targeted gene deletion strategy to investigate individual and the combined influence of MoSCAD genes on growth,stress tolerance,conidiation and pathogenicity of the rice blast ***,localization and co-localization results obtained from this study showed that MoScad1 localizes to the peroxisome and endoplasmic reticulum(ER),MoScad2 localize exclusively to the mitochondria while MoScad3 localize partially to mitochondria and peroxisome during all developmental stages of *** mutants showed they are essential in ER-β-*** observation showed that a minimal but significant growth reduction was caused in the deletion mutants ΔMoscad1 andΔMoscad2,while,growth characteristics exhibited by the deletion mutant ΔMoscad3 was similar with the wild-type ***,we observed that deletion of MoSCAD2 resulted in drastic reduction in conidiation,delayed germination,triggered the development of abnormal appressorium and suppressed host penetration and colonization efficiencies of the ΔMoscad1 *** study provides first material evidence confirming the possible existence of ER β-oxidation pathway in *** also infer that mitochondria β-oxidation rather than peroxisomal and ER β-oxidation play an essential role in the vegetative growth,conidiation,appressorial morphogenesis and progression of pathogenesis in ***.

关键词： ER-β-oxidation SCADs lipid peroxidation ROS M.oryzae

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Optimization of fermentation conditions through response surface methodology to enhanced antibacterial metabolite production that Streptomyces sp.1-14 isolated from the Cassava rhizosphere soil

Optimization of fermentation conditions through response sur...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：云天艳 Feng Renjun Zhou Dengbo Pan Yueyun Chen Yufeng Wang Fei Yin Liyan Zhang Yindong 谢江辉 Institute of Tropical Agriculture and Forestry Hainan University Key Laboratory of Biology and Genetic Resources of Tropical Crops Ministry of AgricultureInstitute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Sciences

Streptomyces sp.1-14 isolated from the rhizosphere soil of cassava were evaluated for antibacterial efficacy against Fusarium oxysporum *** race 4（FOC4）.Among 63 strains tested,thirteen recorded potent antibacterial properties and were further screened against eight bacterial pathogens.A strain showing maximum inhibition against all the test pathogens was identified by 16S rDNA sequencing as Streptomyces sp.1-14 and was selected for further *** propagation of Streptomyces 1-14 in soil under simulated conditions,can found that FOC4 had no significant influence on multiplication and survival of Streptomyces 1-14 in soil,but indigenous microorganisms in soil had significant influence on the counts of Streptomyces *** achieve maximum metabolite production was optimized through response surface methodology employing Plackett-Burman,the path of steepest ascent test and Box-Behnken *** composition of the final optimized fermentation conditions was glucose,38.877 g/L;CaCl·2HO,0.161 g/L;temperature,29.97℃;and inoculation amount,8.93%.The optimization resulted antibacterial activity of 56.13%against FOC4,which was 12.33%higher than that before optimization（43.80%）.The results obtained that using response surface methodology to optimize the fermentation medium had a significant effect on the production of bioactive metabolites by Streptomyces *** the other hand,pH,light,storage temperature,*** fermentation must be closely monitored to reduce the formation of fermentation products with reduced antibacterial *** method is useful for further investigation of the production of anti-FOC4 substances,can be used to develop bio-control agents to prevent and control banana fusarium wilt.

关键词： Streptomyces iranensis 1-14 Fusarium oxysporum f.sp.cubense Response surface methodology Bioactive metabolites

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First report of Colletotrichum boninense causing anthracnose of kiwifruit in China

First report of Colletotrichum boninense causing anthracnose...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Deng Lei Pan Hui Chen Meiyan Zhang Shengju Li Li Zhong Caihong Key Laboratory of Plant Germplasm Enement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of Sciences

In the autumn of 2016,typical anthracnose symptoms were observed on kiwifruit leaves of cultivar ' Hongyang' in kiwifruit orchards of Taishun County,Wenzhou City,*** foliar symptoms were characterized by brown irregular-shaped ***,the middle lesion of each leaf spot became gray,while the edge turned dark brown.A small portion(< 1 cm) of symptomatic leaf tissue was surface-sterilized and plated aseptically onto 1.5%potato dextrose agar(PDA).Hyphal-tip from the growing edge of colonies cultured for three days at 25℃ was transferred to PDA to obtain pure *** 5 days at 25℃,Two single-spore isolate(CB-1、CB-2) was recovered on *** colony of the isolate that grew on PDA had white margins with circular,dull orange *** conidia were hyaline,ellipsoid and cylindrical,obtuse at both ends,(10.3～ 13.5) μm×(4.4～5.8) μ*** upon these characteristics,the causal agent was identified as *** confirm the identity of the isolates,the ITS region of two isolate was obtained and sequenced by PCR amplification using the ITS1/ITS4 universal primers and Col1/ITS4 species-specific *** ITS sequences were identical(Accession ***314166 and MF314167) sharing 100%homology with the sequence of *** extype strain S38 in GenBank(Accession ***076585).Pathogenicity of the isolate was confirmed by inoculating 12 wounded/non-wounded leaves of kiwifruit cultivar ' Hongyang' with a droplet(10μL) of conidial suspension(10～6 conidia/mL) and colonized PDA pieces(5 mm diameter) from 7-day-old cultures of the fungus in Petri *** numbers of control leaves were dropped with 10μL sterile distilled water and agar *** test was performed *** incubation in a controlled environment at25℃ with 80%～85%humidity for 5 days,typical anthracnose symptoms appeared on the wounded inoculated leaves while control and unwounded leaves remained *** was consistently reisolated from symptomatic tissue and confirmed by

关键词： Kiwifruit Anthracnose Colletotrichum boninense

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FgSec2A,a guanine nucleotide exchange factor of FgRab8,is important for polarized growth,pathogenicity and DON production in Fusarium graminearum

FgSec2A,a guanine nucleotide exchange factor of FgRab8,is im...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Zheng Huawei Li Lingping Miao Pengfei Wu Congxian Chen Xiaomin Yuan Mingyue Fang Tian Justice Norvienyeku Li Guangpu Zheng Wenhui Wang Zonghua Zhou Jie State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops College of Plant ProtectionFujian Agriculture and Forestry University Fujian Province Key Laboratory of Pathogenic Fungi and Mycotoxins College of Life SciencesFujian Agriculture and Forestry University College of Ocean Science Minjiang University

Sec4/Rab8 is one of the well-studied members of the Rab GTPase family,findings have shown that Sec4/Rab8 crucially promotes the pathogenesis of phytopathogens,but the up stream regulators of Rab8 are still ***,we identify two Sec2 homologs FgSec2A and FgSec2B in devastating fungal pathogen Fusarium graminearum,and investigate their role and relationship with FgRab8 by live-cell imaging,genetic and functional *** two-hybrid assay shows that FgSec2A specifically interacts with FgRab8 DN(N123I) and ***,FgSec2A is required for growth,conidiation,DON production,and virulence of ***-cell imaging shows that FgSec2A and FgSec2B are localized at the tip region of hyphae and *** N-terminal region and Sec2 domain of FgSec2A are essential for its function,but not for localization,while the C-terminal region is important for its polarized *** active FgRab8 CA(Q69L) partially rescue defects exhibited by the ΔFgsec2A ***,FgSec2A is required for the polarized localization of ***,FgSec2A and FgSec2B have redundant functions,but FgSec2A not interact and colocalize with *** together,these results indicate that FgSec2A acts as a FgRab8 guanine nucleotide exchange factor and is required for polarized growth,DON production and pathogenicity in ***.

关键词： FgSec2 Guanine nucleotide exchange factor Sec4/Rab8 DON Pathogenicity Fusarium graminearum

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蛭石基质中微型薯疮痂病防治药剂的筛选

蛭石基质中微型薯疮痂病防治药剂的筛选

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：邱雪迎李寿如刘红博赵伟全刘大群河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心辽宁本溪市马铃薯研究所中国农业科学院研究生院

马铃薯是小麦、水稻和玉米后的第四大主粮作物,在我国粮食安全、农民增收和保证农业可持续发展等方面具有重要作用。随着我国马铃薯种植规模的不断增长,病害对马铃薯生产的影响日益突出,尤其是各类土传病害传播蔓延的速度明显加快。目... 详细信息

马铃薯是小麦、水稻和玉米后的第四大主粮作物,在我国粮食安全、农民增收和保证农业可持续发展等方面具有重要作用。随着我国马铃薯种植规模的不断增长,病害对马铃薯生产的影响日益突出,尤其是各类土传病害传播蔓延的速度明显加快。目前马铃薯疮痂病作为典型的土传与种传病害,已在我国各马铃薯主产区普遍发生。为探索马铃薯疮痂病的防治方法,本研究在蛭石基质微型薯繁育过程中进行了疮痂病防治试验。首先对微型薯疮痂病斑进行菌株分离纯化,经科赫氏法则验证致病性后,采用CTAB法提取3个菌株的基因组DNA,采用链霉菌16S rRNA基因的通用引物16S1:5′-CATTCACGGAGAGTTTGATCC-3′,16S2:5′-AGAAAGGAGGTGATCCAGCC-3′进行扩增,回收测序后,通过构建系统发育树对菌株进行分子鉴定,确定3个菌株均为疮痂病原菌***。采用纸碟法对29种不同药剂进行皿内抑菌筛选,获得了效果较好的8种药剂,将其与2种酸处理和ZWQ-1生防菌剂同时进行微型薯苗床防治试验。结果表明,在本研究使用的测试药剂浓度条件下,在测试的11个处理中,ZWQ-1菌剂和福美双处理在收获前植株长势良好,无死亡现象发生;72%农用链霉素、咯菌腈、百菌清、柠檬酸和四霉素等处理均出现明显的影响,植株出现黄化,部分死亡,但后期会新生部分叶片;EDTA、亿度勇、氰烯菌酯和三唑酮处理植株均有大量植株死亡。微型薯收获后,经过对薯块数量和病害防治情况进行统计,清水对照收薯189粒,发病率为70.90%;咯菌腈和福美双收薯122粒和120粒,减产明显,但对病害的防治效果较好,分别为72.60%和57.92%;ZWQ-1菌剂、百菌清和柠檬酸处理收薯198粒、216粒和123粒,防效均较低,分别为33.26%、23.68%和19.11%;其余处理由于薯块收获粒数过少均不适合用于病害防治。综合几种处理的表现,福美双和ZWQ-1菌剂对植株的生长有促进作用,咯菌腈对植株生长有一定影响,因此结合使用这3种处理方法也许可以为蛭石基质苗床中***引起的微型薯疮痂病的防治探索一种有效的方法。尽管疮痂病的防治较为困难,但在广大研究者们的不懈努力下,充分结合各项有利因素,一定会探索出有效的防治方法,为马铃薯的优质生产提供有力保障。

关键词：马铃薯疮痂病 S.scabies 药剂筛选蛭石基质防治试验

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中蔗系列甘蔗品种耐寒性及抗黑穗病评价

中蔗系列甘蔗品种耐寒性及抗黑穗病评价

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：张姗姗张沛然兰仙软李茹陈保善广西大学生命科学与技术学院亚热带农业生物资源保护与利用国家重点实验室

甘蔗是一种多年生的宿根热带植物,是我国食糖的主要来源之一。我国蔗区主要分布在广西、广东、云南、海南和福建,寒害和黑穗病一直是影响甘蔗产量的重要因素。广西历年出现不同程度的低温霜冻寒害,造成不同品种蔗苗死亡,导致产量降低。... 详细信息

甘蔗是一种多年生的宿根热带植物,是我国食糖的主要来源之一。我国蔗区主要分布在广西、广东、云南、海南和福建,寒害和黑穗病一直是影响甘蔗产量的重要因素。广西历年出现不同程度的低温霜冻寒害,造成不同品种蔗苗死亡,导致产量降低。另外,宿根蔗的黑穗病发病情况严重,广西崇左市扶绥县以ROC22和GT42发病最为严重,而新植蔗种植成本较高,因此选择高抗黑穗病的甘蔗品种尤为重要。中蔗1号、中蔗6号和中蔗9号为本实验室自育品种,田间综合表现优良。本研究以中蔗1号、中蔗6号、中蔗9号和田间主栽品种ROC22为材料,分别进行芽期0℃不同时长冷冻处理和苗期低温处理,通过芽期萌芽率与苗期最大光化学效率(Fv/Fm)、电导率、叶绿素、丙二醛、脯氨酸、可溶性糖、可溶性蛋白含量和SOD、POD酶活性综合评价4个参试甘蔗品种的耐寒性。实验结果表明,种茎经0℃冷冻处理0～6h,4个甘蔗品种萌芽率均稳定在90%左右,品种间没有明显差异。0℃冷冻处理9h后,中蔗1号、中蔗6号、中蔗9号和ROC22萌芽率分别为22.22%、48.15%、74.19%和100%。当种茎于0℃冷冻处理12h后,中蔗1号萌芽率为零,而中蔗6号、中蔗9号和ROC22的萌芽率分别为3.70%、61.29%和74.07%。苗期低温处理结果也表明中蔗9号较中蔗1号、中蔗6号更为耐寒,与ROC22耐寒能力相当但恢复能力更强。综上结果表明,中蔗系列中中蔗9号最为耐寒。为了评价3个中蔗系列品种对甘蔗黑穗病的抗性,我们用4份来源不同的黑穗病菌单倍体配合悬浮液注射接种蔗芽,统计不同品种的发病潜伏期、发病率,结合大田调查结果进行黑穗病抗性评价。中蔗1号、中蔗6号、中蔗9号和ROC22经人工催芽注射接种后发病潜伏期分别为67d、77d、81d和62d(以第一株发病时间计),发病率分别为42.85%、37.50%、42.85%和71.43%。大田调查发现,中蔗1号、中蔗6号、中蔗9号和ROC22一年宿根黑穗病发病率分别为0、0、0.48%和5.71%(以丛计算)。综上结果表明,中蔗系列整体较ROC22对甘蔗黑穗病具有更强的抗性。

关键词：中蔗系列甘蔗低温胁迫耐寒性甘蔗黑穗病

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Effect of phosphate and potassium solubilizing bacteria on tobacco plant growth

Effect of phosphate and potassium solubilizing bacteria on t...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Sabai Thein Zhang Chengkang Wang Shumin Liu Hong Stefan Olsson Wang Zonghua College of Resource and Environmental Science Fujian Agriculture and Forestry University Department of Botany University of Yangon College of Plant Protection Fujian Agriculture and Forestry University

In this research,investigation of（6） phosphate and potassium solubilizing bacteria from the total of（37） strains uiat isolated from（4） different soil samples of *** solubilizing activities of isolated bacteria were analyzed qualitative and qualitative by agar and broth media as PVK,NBRIP for phosphate solubilizing and AKD for *** the（6） solubilizing strains,S was represented the maximum solubilizing abilities in broth media of PVK,NBRIP（48.71μg/mL,59.66μg/mL） and AKD media（20.49μg/mL）.S16 showed as the second highest activities in PVK（36.42μg/mL）,NBRIP（38.10μg/mL） and AKD（24.00μg/mL）.The sugar fermentation,protease enzyme and siderophore production tests were supported the P,K solubilizing activities of selected *** the basic of 16S rRNA gene sequencing,all（6） strains were closely related widi each ***（4） strains such as S,S,S and S were represented as Bacillus thuringiensis and（2） strains S and S were observed as Lysinibacillus *** examination with gyrB gene sequencing processes,S,S,Sand S were also shown as Bacillus thuringiensis serovar poloniensis,Bacillus thuringiensis serovar kenyae,Bacillus thuringiensis serovar nigeriensis and Bacillus thuringiensis serovar ***,Swas associated with Lysinibacillus sphaericus and S was Lysinibacillus *** to the analyzed of solubilizing acitivites and phylogenetic analysis,S31（Lysinibacillus sphaericus） indicated that the highest P,K solubilizing bacteria among the other ***,the isolated bacteria S16,S31,（S16 + S31） and Sbm（positive control） were used as biofertilizer effect on tobacco plant growth in green *** different ratio of chemical fertilizer and bacteria were utilized to determine the effect of PSB,KSB on tobacco plant *** this experiment,（17） treatments were designed with（3） *** this experiment,plant parameters（plant height,total leaves area and plant biomass） were *** plant samples an

关键词： Effect of phosphate and potassium solubilizing bacteria on tobacco plant growth AKD

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假禾谷镰孢菌全基因组分泌蛋白的预测

假禾谷镰孢菌全基因组分泌蛋白的预测

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者：王利民张银山张梦娟丁胜利李洪连河南农业大学植物保护学院

假禾谷镰孢菌(Fusarium pseudograminearum)是引起小麦茎基腐病的主要病原菌,2012年本课题组在国内首次发现并报道该病原菌,并对该病原菌做了一部分工作。本课题对引起小麦茎基腐的一株高致病力菌株-假禾谷镰孢菌WZ-8A进行了全基因组测... 详细信息

假禾谷镰孢菌(Fusarium pseudograminearum)是引起小麦茎基腐病的主要病原菌,2012年本课题组在国内首次发现并报道该病原菌,并对该病原菌做了一部分工作。本课题对引起小麦茎基腐的一株高致病力菌株-假禾谷镰孢菌WZ-8A进行了全基因组测序,通过多个软件对假禾谷镰孢菌的分泌蛋白组做了初步的预测分析。根据该本地菌株WZ-8A全基因组测序结果,假禾谷镰孢菌共有12 342个编码蛋白的基因。通过信号肽预测软件SignalP v4.1分析,发现其中编码含有信号肽的基因有1 266个,进一步通过Protcomp-v9.0分析编码蛋白的亚细胞定位,752个蛋白具有胞外分泌定位信号,TMHMM-v2.0分析结果显示,744个蛋白不含有或含有1个跨膜域。将744个预测的分泌蛋白通过big-PI Predictor程序进行锚定蛋白的预测分析,688个为非GPI锚定蛋白。最后通过TargetP-v1.1程序进一步排除非胞外分泌蛋白,共有678个蛋白含胞外定位信号。通过上面5个软件的预测,最终预测有678个基因编码分泌蛋白,约占全基因组的5.5%。于钦亮等根据网站已公布的禾谷镰刀菌基因组11640个蛋白质氨基酸序列,通过SignalP v3.0、TargetP v1.01、Big-PI Predictor和TMHMM v2.0四个分析软件预测出606个潜在的分泌蛋白编码基因,约占全基因组的5.2%。陈继圣等对11108个稻瘟菌的ORF进行分析,通过SignalP v3.0、Protcomp-v6.0、TMHMM-v2.0、big-PI Predictor、TargetP-v1.1软件分析,预测出共有1235个ORF可编码分泌蛋白,约占全基因组的11.1%。假禾谷镰孢菌的分泌蛋白组与禾谷镰刀菌基本相似,与稻瘟病菌比较分泌蛋白组在全基因组中占的比例较少,可能与病原菌的种属差异和侵染方式的不同有关。总之,12 342个假禾谷镰孢菌基因所编码的产物通过Signa1P、Protcomp-v9.0、TMHMMv2.0、big-PI Predictor和TargetP-v1.1等程序分析,有678个满足分泌蛋白的特点。因此,初步推测假禾谷镰孢菌全基因组中有678个基因编码的产物为分泌蛋白。本课题组将对预测的分泌蛋白,进一步通过实验验证其预测的可靠性,并对分泌蛋白进行功能分析,期望获得与致病因子相关的分泌蛋白和激发子,并对其功能加以深入的研究。

关键词：禾谷镰孢菌分泌蛋白组全基因组测序基因编码分泌蛋白全基因组

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First report of seedborne and stem necrosis caused by Stenotrophomonas maltophilia on biomes energy plant(Jatropha curcas L.) in China

First report of seedborne and stem necrosis caused by Stenot...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Wang W.W. Yang J.J. Tang W. Ye X.Y. School of Life Science Yunnan Normal University Joint Academy of Potato Science Yunnan Normal University

Barbados Nut(Jatropha curcas L.) is one of the most important sustainable energy plant and mainly distributed to tropical and subtropical zone around the *** plant is also grows in southwestern China,especially in Yunnan,Sichuan and Guizhou *** seeds contained higher oil up to 60%,and has a broad prospect for biomass *** July 2017,a new disease was observed on seeds brought from local market which harvested last ***,the seeds germination rate almost decreased to 20%-30%when we induce with 1.3%CuSO disinfection(30 minutes),washed twice by stilled water,placed on sterilizing filter paper and maintained in 24℃.The discolor progressed over time and finally the whole seeds became yellowish *** germinated but infected plants also showed stem yellow *** many case,stems and roots developed a necrosis symptom within 3-5 days after *** strains were isolated from symptomatic seeds and stems by dilution plating on LB medium cultured in 24℃.Morphological test showed Gram-negative and rod shaped and motile with a few polar flagella(1).Total genomic DNA was extracted from 5 strains of bacteria、 2 asymptomatic seeds and 5 healthy stems by using bacterial DNA kit(DP302,TIANGEN).PCR was carried out with bacterial universal primer set 27 F/1492 R followed by(2),resulting in DNA fragment that were 1.5 kb,respectively,all asymptomatic samples were not *** product were cloned into a pGEM-T Easy vector(Promega) and *** analysis in NCBI shared 99%and above similarity with that species of Stenotrophomonas maltophilia(GenBank Accession ***268318.1).Phylogenetic tree based on Stenotrophomonas *** combine other distinct genus 16S rDNA analysis also proved the sequence belong to *** sub *** test pathogenicity,the healthy seeds were immersed in bacterial suspension(10 CFU/liter) in 30 minutes,and inducing germination as above,meanwhile,the plant stems were also inoculated by spray wit

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Lignin metabolism involves Botrytis cinerea BcGsl-induced defense response in tomato

Lignin metabolism involves Botrytis cinerea BcGsl-induced de...

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中国植物病理学会第十一届全国会员代表大会暨2018年学...

作者： Yang Chenyu Liang Yingbo Qiu Dewen Zeng Hongmei Yuan Jingjing Yang Xiufen Key Laboratory of Control of Biological Hazard Factors(Plant Origin) for Agri-product Quality and Safety Ministry of AgricultureInstitute of Plant Protection

BcGs1,a cell wall degration enzyme(CWDE) is originally derived from Botrytis *** previous study revealed BcGs1 could trigger defense response and protect plants against various *** this study,two domans of Glyco-hydro 15(GH15) and CBM20lucoamylase(CBM20) of BcGs1 were transiently expressed in Nicotiana benthamiana and the results revealed that the two domains were required for BcGs1 full necrosis *** best understand the defense response mechanism underlying this BcGs1 elicitation in tomato,the differential protein expression profiles between BcGs1 and TrisHCl buffer treatment plants was performed using the iTRAQ-based quantitative proteome *** 71 up-regulated with ratio >1.3 and 38 down-regulated proteins with ratio <0.77 were *** analyses and functional assignments indicated a significant expression complexity in differentially expressed *** up-regulated proteins,major was focus on pathogenesis-related protein,peroxidases,Glucan endo-1,3-β-glucosidase,chitinase,biosynthesis of secondary *** real-time PCR(qPCR) was performed to investigate gene transcriptional profile post BcGs1 *** genes encoding above differential proteins were expressed with 5-20 folds *** to the up-regulated proteins and genes,oxidative metabolism and phenylpropanoid metabolism were speculated to be involved in BcGsl-triggered defense response in ***,the experimental evidence showed BcGsl triggered ROS burst,increased level of phenylalanine-ammonia lyase(PAL) and peroxidase(POD)enzyme activity and lignin ***,histochemical analysis revealed that BcGsl infiltration tomato leaves exhibited cell wall thickness compared with untreated ***,BcGs1 activated basal defense response and lignin metabolism contributed to BcGs1-induced resistance to *** infection in tomato.

关键词： Fungal protein elicitor Defense response Phenylpropanoid Lignin Botrytis cinerea

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