Cucumber green mottle mosaic virus(CGMMV) is an important pathogen of cucumber(Cucumis sativus).The molecular mechanisms mediating host-pathogen interactions are likely to be strongly influenced by microRNAs(miRNAs),w...
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Cucumber green mottle mosaic virus(CGMMV) is an important pathogen of cucumber(Cucumis sativus).The molecular mechanisms mediating host-pathogen interactions are likely to be strongly influenced by microRNAs(miRNAs),which are known to regulate gene expression during the disease *** current study focused on six known miRNAs(miR159,miR169,miR172,miR838,miR854 and miR5658),eight novel ones(csa-miRnl-3p,csa-miRn2-3p,csa-miRn3-3p,csa-miRn4-5p,csa-miRn5-5p,csa-miRn6-3p,csa-miRn7-5p and csa-miRn8-3p) and their target *** data collected was used to construct a regulatory network of miRNAs and target genes associated with cucumber-CGMMV interaction,which identified 608 potential target genes associated with all the miRNAs except *** of the miRNAs including miR159,miR838,miR854,miR5658 and csamiRn6-3p,were found to be mutually linked by target genes,while another eight including miR169,miR172,csa-miRnl-3p,csa-miRn2-3p,csa-miRn3-3p,csa-miRn4-5p,csa-miRn5-5p and csamiRn8-3p formed sub-networks that did not display any connectivity with other miRNAs or their target *** transcript quantitative real-time PCR(RT-qPCR) was used to analyze the expression levels of the different miRNAs and their putative target genes in leaf,stem,and root samples of cucumber over a 42-day period post-inoculation with CGMMV.A positive correlation was found between some of the miRNAs and their respective target genes,although for most the response varied greatly depending on the time point post inoculation,which indicated that additional factors are likely to be involved in the interaction between cucumber miRNAs and their target *** miRNAs,including miR159 and csamiRn6-3p were linked to target genes that have been associated with plant responses to disease and a hypothetical model linking miRNAs,their targets and downstream biological processes was proposed to indicate the role of these miRNAs in the cucumber-CGMMV interaction.
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