Plus end tracking proteins(TIPs) are a unique group of microtubule binding proteins that dynamically track microtubule(MT) plus *** is a highly conserved TIP with a fundamental role in MT dynamics,but it remains poorl...
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Plus end tracking proteins(TIPs) are a unique group of microtubule binding proteins that dynamically track microtubule(MT) plus *** is a highly conserved TIP with a fundamental role in MT dynamics,but it remains poorly understood in fungal *** to the previous study,we propose that the alterations in α-and β-tubulin of fungal pathogen has changed the interaction between EBl and microtubule which enhanced the resistance to drugs inhibit microtubule *** order to figure out this problem,we expressed His-tagged EBl and GST-tagged EBl in *** two recombinant EBl were purified by Ni and GSH sepharose affinity chromatography,*** yields of two recombinant EBl were 4 and 6 mg/L,respectively,with 90%***-Pull down demonstrated that no interactions between EBl and tubulin monomers were ***,EBl can enhance the polymerization of microtubule in ***,the co-localization of EBl and microtubule were observed by GFP-labeling technic and this result indicated that EBl may bind to the polymerized *** all,these results are helpful for us to better understand the role of EBl in microtubule assembly.
KH domain-containing proteins are widely conserved and play diverse roles in post-transcriptional gene ***,few studies deal with their roles in pathogenesis of plant pathogenic ***,we identified a novel virulence gene...
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KH domain-containing proteins are widely conserved and play diverse roles in post-transcriptional gene ***,few studies deal with their roles in pathogenesis of plant pathogenic ***,we identified a novel virulence gene PCG6 that encodes a protein with five KH domains in the rice blast fungus Magnaporthe *** of PCG6 led to severely attenuated virulence toward host *** observation showed that the deletion mutant was reduced in appressorium formation,penetration peg formation,and development of invasive *** addition,the PCG6 deletion mutant was also significantly reduced in vegetative hyphal growth and ***6 encodes a cytoplasmic protein expressing in mycelia,conidia,appressoria,and infection ***,Pcg6 was co-purified and physically interacted with several proteins involved in nonsense mediated mRNA decay(NMD).Interestingly,the expression level of GLD1,a gene for glutamate dehydrogenase,was markedly increased in the PCG6 deletion mutant as compared with the wild ***,over-expression of GLD1 in the wild-type strain exhibited similar phenotypes as the PCG6 deletion *** together,we proposed that the multiple KH domain protein Pcg6 regulates the glutaminolysis by NMD to control infection-related morphogenesis and developments in the rice blast fungus.
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