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检索条件"任意字段=中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会"

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大豆孢囊线虫3号和4号生理小种不同龄期差异表达基因分析

大豆孢囊线虫3号和4号生理小种不同龄期差异表达基因分析

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：王高峰彭德良高丙利黄文坤孔令安龙海波彭焕简恒中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室中国农业大学植物病理系中国科学院上海生命科学研究院湖州现代农业生物技术产业创新中心中国热带农业科学院环境与植物保护研究所热带作物虫害综合治理重点实验室

大豆孢囊线虫Heterodera glycines是大豆上重要病源线虫,而有关大豆孢囊线虫不同生理小种与寄主互作机理差异的研究仍十分缺乏。本研究通过分析大豆孢囊线虫3号与4号生理小种同大豆高感品种中黄13互作阶段的转录水平差异来探讨3号与4号... 详细信息

大豆孢囊线虫Heterodera glycines是大豆上重要病源线虫,而有关大豆孢囊线虫不同生理小种与寄主互作机理差异的研究仍十分缺乏。本研究通过分析大豆孢囊线虫3号与4号生理小种同大豆高感品种中黄13互作阶段的转录水平差异来探讨3号与4号生理小种对大豆寄主中黄13的应答差异。最终,应用有限的线虫材料通过抑制性消减杂交技术分别构建了大豆孢囊线虫侵染后二龄、三龄和四龄雌虫这3个龄期共6个cDNA抑制消减文库。在这6个cDNA抑制消减文库中共发现689个候选的差异表达基因。通过qRT-PCR分析最终确定92个为3号与4号生理小种之间的差异表达基因,其中包括8个效应蛋白编码基因。并且,在3号与4号生理小种寄生阶段后期鉴定到更多的差异表达基因。对侵染后三龄和四龄雌虫阶段的差异表达基因进行GO分析后发现,这些基因的功能主要涉及线虫的物质代谢。此外,以其中包括8个效应蛋白编码基因在内的13个差异表达基因为研究对象,应用qRT-PCR技术比较分析了这13个基因在3号与4号生理小种侵染前和侵染后二龄、三龄以及四龄雌虫这4个不同发育阶段的相对表达量,结果发现这些基因的表达丰度在3号与4号生理小种的一个或多个龄期表现差异,包括侵染前二龄阶段。同时,比较分析这13个基因在3号与4号生理小种间的发育表达模式后发现,其中有6个基因的发育表达趋势在这两个生理小种间存在差异,这一现象首次在大豆孢囊线虫中观察到。

关键词：大豆孢囊线虫生理小种转录水平差异 cDNA抑制消减文库

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黄瓜绿斑驳花叶病毒葫芦上的种传规律及热处理效果评价研究

黄瓜绿斑驳花叶病毒葫芦上的种传规律及热处理效果评价研究

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：宋顺华宫国义耿丽华吴萍孟淑春邢宝田北京市农林科学院蔬菜研究中心/农业部华北地区园艺作物生物学与种质创制重点实验室/农业部都市农业(北方)重点实验室

黄瓜绿斑驳花叶病毒（CGMMV）以带毒种子为主要初侵染源,给我国西甜瓜生产造成了严重的威胁。在西瓜大量采用嫁接栽培技术的生产方式下,该病毒主要通过砧木种子带毒传播,西瓜种子很少带毒,甚至不带毒。加强砧木种子的带毒检测和使用有... 详细信息

黄瓜绿斑驳花叶病毒（CGMMV）以带毒种子为主要初侵染源,给我国西甜瓜生产造成了严重的威胁。在西瓜大量采用嫁接栽培技术的生产方式下,该病毒主要通过砧木种子带毒传播,西瓜种子很少带毒,甚至不带毒。加强砧木种子的带毒检测和使用有效方法处理种子可以从源头控制该病害的蔓延和流行,是防控CGMMV的关键措施。本研究以带毒的葫芦种子作为材料,采用DAS-ELISA、RT-PCR与生物学检测相结合的方法检测葫芦种子的带毒率和传毒率,评价干热处理对病毒的钝化效果。6个批次的葫芦种子有5个批次种子阳性,带毒率在14%00%,贮藏1年后传毒率在2.3%.6%。DAS-ELISA灵敏度检测种子带毒量在感染种子研磨液与健康种子研磨液的体积比例为1:1 000时仍能检测出阳性。干热处理6个批次的葫芦商品种子（5个品种）,其中4个批次的种子为CGMMV阳性,只有1个批次阳性种子的实生苗出现1株阳性。本研究表明:RT-PCR检测比DAS-ELISA灵敏度高,两种方法均能从种子浸泡液准确的检测葫芦种子的带毒情况,使之应用检测大批量商品葫芦种子更加简便可行;种传率的高低与种子的带毒率有关,葫芦种子的种传率低而带毒率高,但收获1年以后的种传率与其他研究的新鲜葫芦种子相差不大,说明葫芦种子以表面带毒为主,而且非常稳定;72℃/72h干热处理种子能有效的钝化CGMMV,防治效果显著,但其防效没有100%,还应结合使用其他防治方法;生物学检测方法可以检测病毒的传染性,证明病毒与病害发生的直接相关性,是种子处理后的唯一检测方法;CGMMV在葫芦作物上的隐性带毒现象非常普遍,这在病害的防控上是一个严重的安全隐患,也是限制生物学检测方法应用的一个瓶颈,必须结合室内其他鉴定方法以提高检测结果的准确性。

关键词：黄瓜绿斑驳花叶病葫芦种子带毒率传毒率干热处理

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Molecular Design and Synthesis of Salicyl Glycoconjugates as Plant Activators

Molecular Design and Synthesis of Salicyl Glycoconjugates as...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Cui Zining Jun Ito Yoshimiki Amemiya Yoshihiro Nishida Li Ying Zhang Lianghui Guangdong Province Key Laboratory of Microbial Signals and Disease Control Department of Plant PathologyCollege of Natural Resources and EnvironmentSouth China Agricultural University State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Graduate School of Horticulture Chiba University College of Chemistry and Material Science Shandong Agricultural University

During the long process of co-evolution,plants had evolved lots of defense mechanisms to deal with pests and *** plant-pathogen interaction,a number of plant defense responses could be induced(e.g.,callus deposition,P... 详细信息

关键词： Molecular Design and Synthesis of Salicyl Glycoconjugates as Plant Activators

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Biocontrol of Litchi Downy Blight(Peronophythora litchi) by Zeamines,a New Type of Phytotoxin Producing by Dickeya zeae

Biocontrol of Litchi Downy Blight(Peronophythora litchi) by ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Liao Lisheng Liu Shiyin Lv Mingfa Gu Yanfang Zhou Jianuan Chen Shaohua Zhang Lianhui Guangdong Province Key Laboratory of Microbial Signals and Disease Control College of Natural Resources and EnvironmentSouth China Agricultural University Institute of Molecular and Cell Biology Department of Biological Sciences National University of Singapore

Absract:The antifungal activity of a new type of long chain aminated polyketide named zeamines(ZMS),against Peronophythora litchi both in vitro and in vivo was *** treatment significantly suppressed mycelial growth and spore germination of *** in *** at 5|xg/mL inhibited95.8%of the germination of *** *** results from scanning electron microscopy(SEM) and transmission electron microscopy(TEM) showed ZMS caused the ultrastructural modifications of ***,including the distortion and depression of cell wall and endomembrane system,especially the plasma membrane,mitochondria,and vacuoles,which resulted in the cellular integrity ***,utilizing of ZMS significantly(P < 0.05) reduced decay and subdued peel browning of postharvest litchi fruit inoculated with *** during storage at ***,these results suggested that ZMS showed outstanding antifungal activity against *** both in vitro and in vivo,which might provide a new strategy for the control of postharvest decay of litchi fruit.

关键词： Zeamines Antifungal activity Peronophythora litchi Dickeya zeae

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Genome Sequence and Molecular Analysis of an Isolate of the Bean Common Mosaic Virus from Hyacinth Bean Plants in Shandong

Genome Sequence and Molecular Analysis of an Isolate of the ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Su Wenmin Liu Aixin Li Gang Zhao Liming Li Dongxiang Zhu Xiaoping Department of Plant Pathology College of Plant ProtectionShandong Agricultural University

Potyviruses are responsible for substantial losses to bean crop farmers in many parts of the *** such virus,the bean common mosaic virus(BCMV),has been isolated from hyacinth bean leaves in the Tai'an region of the Shandong province of *** complete genomic sequence of this isolate,which we have called BCMV-HB,was obtained and then used to conduct molecular genetic analyses to infer its taxonomic relationship with other members of the *** results indicate that the BCMV-HB isolate is phylogenetically distinct from other strains of the BCMV *** data also suggest that the taxonomic status of the BCMV-HB isolate falls somewhere between peanut strip virus and other BCMV *** addition,recombination was identified in the nuclear inclusion ' b' protein region of the BCMV-HB *** results of this study indicate that BCMV-HB,which infects hyacinth bean plants,is a new strain of BCMV.

关键词： Bean common mosaic virus Potyviruses Hyacinth bean China Complete genome sequence

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Molecular Detection and Complete Genome Sequences of Tomato Chlorosis Virus Isolates from Infectious Outbreaks in Shandong

Molecular Detection and Complete Genome Sequences of Tomato ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhao Liming Li Gang Gao Ying Liu Yongjie Sun Guozhen Zhu Xiaoping College of Plant Protection Shandong Agricultural University

Tomato chlorosis virus(ToCV) is a whitefly-transmitted,phloem-limited,bipartite *** 2012,severe interveinal symptoms characteristic of ToCV infections were observed in greenhouse tomato plants in the Shandong *** levels of infestation by whiteflies(Bemisia tabaci),which transmit ToCV,were also observed on tomato plants in all the greenhouses *** presence of ToCV was confirmed by specific RT-PCR either in the sampled plants or in the whiteflies collected from the ventral surface of the leaves of diseased *** complete genomic nucleotide sequences(RNA1 and RNA2) of the Shandong isolate of ToCV(ToCV-SDSG) were determined and ***-SDSG RNA1 consisted of 8594 nucleotides encompassing four open reading frames(ORFs).ToCV-SDSG RNA2 consisted of 8242 nucleotides encompassing nine *** analysis suggests that the Chinese ToCV-SDSG isolate is most similar to the ToCV-florida isolate.

关键词： China Crinivirus Tomato Tomato chlorosis virus

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Microscopic Examination of Developmental and Infection Processes with a GFP labelled Taphrina deformans Transformant

Microscopic Examination of Developmental and Infection Proce...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhang Yimei Liu Huiquan Zhang Qiang Xu Jinrong Purdue-NWAFU Joint Research Center and State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University

Taphrina deformans is a biotrophic pathogen that causes peach leaf curl,a worldwide disease that is potentially devastating to both yield and tree *** has a dimorphic life cycle with a hyphal stage and a yeast *** yeast phase resulting from budding of ascospores is saprophytic and can be cultured on artificial *** hyphal form is associated with plant infection and obligate *** the underlying mechanism is not clear,dimorphic switching in this homothallic fungus is assumed to be related to sexual reproduction and *** a plant pathogen in Taphrinomycotina,*** has great potentials for being used to study mechanisms of fungal-plant interactions and evolution of effectors and *** this study,we tested with different transformation protocols and developed an electroporation transformation procedure with *** transforming vectors contained the GFP reporter gene either fused to Hi-histone gene of *** or under its promoter *** transformation efficiency was compared with different forms of transforming *** resulting transformants were used to examine nuclear behaviors during blastospore germination and dimorphic *** hyphae growing in peach tissues also were examined with leaves inoculated with GFP-labelled txansformants.A model depictive of different fungal development and infection stages of *** will be presented.

关键词： Taphrina deformans Dimorphism Transformation

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Characterization and Genetic Analysis of a Rice Mutant Exhibiting Compromised Non-host resistance to Puccina striiformis ***(Pst)

Characterization and Genetic Analysis of a Rice Mutant Exhib...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhao Jing Yang Yuheng Xing Huijun Zhan Gangmin Kang Zhensheng State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University

Nonhost resistance is the most basic and common form of plant *** efforts have focused on nonhost resistance owing to its broad spectrum and *** previous studies,we got a rice mutant crrl（compromised resistance to rust 1） that was more vulnerable to Puccina striiformis ***（Pst）,the causal agent of wheat stripe rust More and larger infection sites were produced on crrl compared with wild type Nipponbare when inoculated with *** analysis revealed that the mutant phenotype was controlled by one locus localized between molecular marker R.M25761 and RM25792 on chromosome *** hydrogen peroxide（HO） was detected at the infection sites on crrl as well as wild plants,the invasion and growth of Pst were not affected by the production of HO in the *** implied that HO may act as signal molecules and mutant gene function at downstream of the pathway.

关键词： nonhost resistance rice mutant stripe rust hydrogen peroxide

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Role of Salicylic Acid Pathway in N-acyl-homoserine Lactones-elicited Plant Resistance

Role of Salicylic Acid Pathway in N-acyl-homoserine Lactones...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Liu Fang Zhao Qian Huang Yali Song Shuishan Biology Institute Hebei Academy of Sciences and Hebei Engineering and Technology Center of Microbiological Control on Main Crop Disease

Many gram-negative bacteria use N-acyl-homoserine lactones(AHL) as quorum-sensing signals to coordinate their collective *** evidence indicates that plants can respond to ***,little is known about the molecular mechanism of plants reacting to these bacterial *** we show that treatment of Arabidopsis roots with the N-3-oxo-octanoyl-homoserine lactone(30C8-HSL) significantly increased plant resistance to infection of pathogen bacteria Pseudomonas syringae *** DC3000.30C8-HSL treatment enhanced the concentration of salicylic acid,followed by a reactive oxygen burst,as well as the expression of PATHOGENESIS-RELATEDl *** contrast to wild type Arabidopsis,nprl mutant and the transgenic lines nahG are compromised in the AHL effect,suggesting that systemic acquired resistance(SAR) is required for AHL-induced *** data showed that AHLs-elicited resistance depends on cross-talk with plant hormones salicylic acid pathway.

关键词： N-acyl-homoserine lactones Salicylic acid Plant resistance Cross-talk

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Population Genetic Structure of Phytophthora infestans in Fujian,China

Population Genetic Structure of Phytophthora infestans in Fu...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhu Wen Yang Lina Shang Liping Zhan Jiasui Key Lab for Biopesticide and Chemical Biology Ministry of EducationFujian Agriculture and Forestry University Fujian Key Lab of Plant Virology Institute of Plant VirologyFujian Agriculture and Forestry University

Late blight,caused by ***,is the most distinctive disease of potato(Solanumtuberosum).This study was aimed to understand the population genetic structure of Phytophthora infestans in Fujian,China,and inferred the evolutionary mechanisms governing the structure.534 isolates were sampled from eight locations in Fujian province from 2010 to 2012 and assayed the isolates with eight simple-sequence repeat(SSR) markers.30 alleles and 49 genotypes were detected among the 534 *** analyses reveal that *** populations in Fujian province are made of three differentiated genetic *** A were self-fertility,while cluster B include mating types A1 and self-fertility,cluster C include only mating types *** analysis indicated that Cluster C were likely to have experienced a recent population *** propose that genetic variation of *** population in Fujian is due to the infected seeds imported from other parts of *** implications of these results are discussed.

关键词： Phytophthora infestans Genetic diversity Population genetic structure

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