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检索条件"任意字段=中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会"

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The Overwintering Study of the Uromyces appendiculatus Teliospore in Heilongjiang

The Overwintering Study of the Uromyces appendiculatus Telio...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zheng Sujiao Li Jili Ke Xiwang Tai Lianmei Zuo Yuhu Heilongjiang Bayi Agricultural University

Adzuki bean rust,caused by Uromyces appendiculatus,has been considered as one of the most serious diseases of adzuki bean in *** investigation in the west of Heilongjiang province showed that the most adzuki bean production areas had nearly 100 percent incidence,serious plot even ***,little information about the primary source of infection and life cycle of *** on adzuki bean in Heilongjiang province has been reported to *** order to clarify whether the teliospore of *** can overwinter in Heilongjiang province,the teliospores were collected at October and monitored the teliospore germination rates,which subjected in different conditions including stored at room temperature,4℃,-20℃,hanging outdoor and underground 10 *** results showed that the teliospore can germinate after treated in the above 5 conditions after 7 months,but with significantly different germination rate(29.9%、 21.2%、 35.2%、 6.8%and 23.1%,respectively).In addition,the results also showed that 27℃.with darkness is suitable for teliospore germination which stored at room temperature,the germination rate is up to 36.7%.In conclusion,the results indicated that teliospore of *** can overwinter in Heilongjiang province,which shed light to clarify the primary source of infection and life cycle of adzuki bean rust,and provide important clues for designing strategies for effective prevention and control of adzuki bean rust.

关键词： Uromyces appendiculatus Teliospore Overwinter

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Control of Phytopathora capsic Causing Pepper Phytopathora Blight by 2-hydroxycycloakylsulfonamides

Control of Phytopathora capsic Causing Pepper Phytopathora B...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： He Ruihong Xiao Shuqin Niu Mengyan Liu Kexin Zhao Tianyu Xue Chunsheng Department of Plant Protection Shenyang Agriculture University

Phytophthora was a kind of wordwide distribution plant pathogen and caused serious *** phytophthora blight was a devastating soil-borne disease caused by Phytophthora *** is one of the useful measures to control Pepper Phytophthora *** this study,20 novel 2-hydroxycycloakylsulfonamides were screened against mycelial growth rate,sporangium formation and zoospore release of Phytophthora *** growth rate of Phytophthora capsici was tested by metalaxyl,flumorph,cyazofamid and 20 novel 2-oxodroxycycloakylsulfonamides.149 and 104 are two of the best compounds that inhibited mycelium growth of Phytophthora capsici,ECvalues of which are 9.58|xg/mL,17.86μg/mL Secondly,we observed the effect of 149 and104 the two compounds on the formation sporangium of Phytophthora *** to the results,both the two compounds demonstrated obvious inhibitory effects on sporangium formation,with the ECvalues of 0.03μg/mL and.4.89μg/mL,respectively.149 showed obvious inhibitory effects than that of *** release inhibitory rate of Phytophthora capsici on glass slides was tested by metalaxyl,flumorph,cyazofamid,149 and *** results showed that 104 exhibited obvious inhibitory effects on zoospores releasing,with the ECvalues of 0.18μg/mL,and it exhibited stronger inhibitory effect than that of metalaxyl control.

关键词： Phytophthora capsici 2-hydroxycycloakylsulfonamides Sporangium Zoospore

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Interaction between Barley yellow dwarf virusGAV and Brachypodium distachyon

Interaction between Barley yellow dwarf virusGAV and Brachyp...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Tao Ye Wu Keke Song Shuang Hao Xingan Wu Yunfeng State Key Laboratory of Crop Stress Biology in Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant ProtectionNorthwest A&F University

Virus-host interaction plays an important role on viral ***,it is difficult to study the interaction of Barley yellow dwarf virus-GAV(BYDV-GAV) and its wheat host in molecular level because of the huge and complex genome of wheat *** further understanding of the molecular mechanism of this progress,a new model plant for cereal,Brachypodium distachyon,was chosen to construct an alternative virus-host interaction *** susceptibility of *** to BYDV-GAV was determined by inoculation of viruliferous aphids into this model *** observation and results of reverse transcription PCR at 14,21 and 35 dpi both indicated the GAV could infect the ***,GAV-host interaction was studied in this system by screening interacted proteins from the cDNA libraries of *** using yeast two hybrid *** different proteins including an annexin D5-like,an uncharacterized protein sharing 85%indentity with OsVOZ and a fructose-bisphosphate aldolase were screened out to interact with CP,MP and P6 of BYDV-GAV,*** further verification of these interactions was still in *** strain of BYDV-GPV,was also studied on the virus-insect interaction using Y2 H and Chemiluminescent co-immunoprecipitation to explain the transmission mechanism of BYDV.

关键词： Interaction between Barley yellow dwarf virusGAV and Brachypodium distachyon

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HrcT is a Key Component of the Type Ⅲ Secretion System in Xanthomonas and also Regulates the Expression of the Key hrp Transcriptional Activator HrpX

HrcT is a Key Component of the Type Ⅲ Secretion System in X...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Liu Zhiyang Zou Lifang Xue Xiaobo Cai Lulu Ma Wenxiu Xiong Li Ji Zhiyuan Chen Gongyou School of Agriculture and Biology and State Key Laboratory of Microbial MetabolismShanghai Jiao Tong University

The type Ⅲ secretion system(T3SS),encoded by hrp(hypersensitive response and pathogenicity) genes in Gram-negative phytopathogenic bacteria,delivers repertoires of T3 SS effectors(T3SEs) into plant cells to trigger the hypersensitive response(HR) in nonhost or resistant host plants and promote pathogenicity in susceptible *** expression of hrp genes in Xanthomonas is regulated by two key regulatory proteins,HrpG and ***,the interactions between hrp gene products in directing secretion are largely *** we demonstrated that HrcT of *** ***(Xoc)functions as a T3 S component and positively regulates the expression of *** of hrcT occurs via two distinct promoters,one is with the hrpB operon(T1) and the second(T3) within *** either Tl or T3 promoter,the defect in Hrp phenotype by hrcT mutant was restored in the presence of hrcT only from Xanthomonas species,but not other phytopathogenic *** N-terminally truncated HrcT was able to bind the hrpX promoter and activate the expression of hrpX,supporting that HrcT is a positive regulator of hrpX.A revised model showing the regulatory interactions between HrcT,HrpX and HrpG is proposed.

关键词： Xanthomonas HrcT HrpX T3SS Regulation Pathogenicity

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The Preliminary Functional Study of Two Hexokinase Gene in Valsa mali

The Preliminary Functional Study of Two Hexokinase Gene in V...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Dai Qingqing Song Na Huang Lili State Key Laboratory of Crop Stress Biology for Arid Areas/College of Plant Protection Northwest A&F University

Vmhxk1,a pathogenicity-related gene predicted to encode hexokinase,was obtained by amplifying the flanking sequence of T-DNA of one pathogenicity weakened ***1,encoding glucokinase,another gene of hexokinase gene family was acquired by the genomic information of *** this study,the two gene Vmhxk1 and Vmgxk1 function were analyzed by gene knockout and complementation *** result showed that Vmgxk1-deficient mutants were indistinguishable from the wild-type in all growth parameters tested except the time of conidia formation which was delayed for 15 *** contrast,△Vmhxk1 mutants lacking Hxkl showed a pleiotropic growth *** artificial media,vegetative growth was retarded,and the time of conidia formation postponed very *** with the wild type 03-8,the pathogenicity of △Vmhxk1 mutants was *** complementation of△Vmhxk1 and △Vmgxk1 mutants showed similar phenotype and pathogenicity to *** results indicated that Vmgxk1 was involved in the conidia formation and Vmhxk1 was not only related to conidia formation,but also mycelium growth and pathogenicity.

关键词： Valsa mali flanking sequence Hexokinase gene knockout pathogenicity analyse

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MiR482 Negatively Regulates the Expression of CC-NBS-LRR Resistance Genes in Tomato

MiR482 Negatively Regulates the Expression of CC-NBS-LRR Res...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Jiang Ning Luan Yushi Sun Guangxin School of Life Science and Biotechnology Dalian University of Technology

NBS-LRR disease-resistant gene family is the most representative family of resistance *** studies have shown that miR482 targetes NBS-LRR mRNA sequences and regulates the immune response in *** study the interaction between different miR482 family members and their target genes in tomato,we downloaded the sequences of three miR482 family members(miR482a,miR482 b,miR482c) from the miRBase,following by the prediction of their target *** to the predicted results and the relative reports,we selected Solyc02g036270.2.1(CC-NBS-LRR) to study the interaction relationship between the target gene and the three miR482 family *** assayed the changes of expression of miR482 family members and their target gene by quantitative real-time PCR after Phytophthora infestans induced for different time *** results of quantitative real-time PCR and correlation analysis indicated various miR482 family members negatively regulate the expression of their common target gene on different *** result further revealed the interaction between the miRNAs and their target genes,providing a new theoretical basis for the breeding of tomato disease resistance.

关键词： CC-NBS-LRR miR482 Tomato Expression characteristic Phytophthora infestans

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The Xanthomonas Hijack Host Basal Transcriptional Factor for its Successful Invading

The Xanthomonas Hijack Host Basal Transcriptional Factor for...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Meng Yuan National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural University

The battle between pathogens and plants is never *** of the parasites and their hosts is the basis of the continuous *** bacteria interact with plants by secreting proteins into host *** proteins,known as effectors and injected into host cells by the type HI secretion system,which is highly conserved in plant pathogens,play an essential role in pathogenicity in plants,and are through to interact with components for host immunity to suppress defence responses and support pathogen growth after translocation into the *** is one of the most widespread phytobacteria,causing diseases on a variety of agricultural plants,it contains different number TAL effectors,which target host specific genes to manipulate the host transcriptional machinery for virulence and/or ***,we found that Xanthomonas TAL effectors using its relatively conserved region by interacting with host basal transcriptional factor for targeting host specific genes to manipulate the host transcriptional for its growth and successful invading,the conserved interaction between Xanthomonas TAL effectors and host host basal transcriptional factor were found in Xanthomonas oryzae ***-rice,Xanthomonas oryzae ***-rice,Xanthomonas axonopodis ***-citrus and Xanthomonas campestris ***-pepper/*** results provide evidence that the interaction complexity between pathogens and plants.

关键词： Xanthomonas Plant-pathogen interaction TAL effector Basal transcriptional factor

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Analysis SSR Information in Coffee Leaf Rust Transcriptome

Analysis SSR Information in Coffee Leaf Rust Transcriptome

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：吴伟怀贺春萍 Zou Haijuan Liang Yanqiong Xi Jingen Zheng Xiaolan Zheng Jinlong Li Rui 易克贤 Environment and Plant Protection Institute CATAS College of Environment and Plant Protection Hainan University

Leaf rust,which is caused by the fungus Hemileia vastatrix,is a devastating disease that affects coffee plants(Coffea arabica L.).In this study,simple sequence repeat identification tool was used to screen SSRs in coffee leaf rust transcriptome.A total of 1292 SSRs were found in coffee leaf rust9234 *** the 1292 SSRs,the most abundant SSR is the tri- nucleotide type,with the SSR numbers being 459,followed by the di- and hexa- nucleotide ones,with 321 and 214 SSR numbers,accounting for 35.5%,24.8%and 16.6%,*** the di-nucleotide repeats,AT/AT was the most motifs and accounted for 49.8%.However,ATC/ATG and AAG/CTT were both the most frequent motif among the tri-nucleotide repeats,accounting for 23.5%and 23.3%,***,the polymorphism of SSRs was assessed by filter out in the length of lower than 22 bp SSR,and 223 potentially polymorphism SSRs were ***,these results are useful to analyze genetic viaration,diversity,and evolution of coffee leaf rust pathogen.

关键词： Coffee leaf rust Transcriptome SSR

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侧孢短芽孢杆菌B8产生胞外抑菌物质对植物病原真菌抑菌机理的研究

侧孢短芽孢杆菌B8产生胞外抑菌物质对植物病原真菌抑菌机理的研究

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：李悦余惠荣李蔚赵秀香沈阳农业大学植物保护学院

芽孢杆菌在植物病害生物防治中应用广泛。近年来,侧孢短芽孢杆菌(Brevibacillus laterosporus)作为一种新型生防菌引起了广泛的关注,目前已证明侧孢短芽孢杆菌具有杀虫、杀线、溶磷、降解有机物及抗肿瘤作用,但是对于其抗菌作用研究较... 详细信息

芽孢杆菌在植物病害生物防治中应用广泛。近年来,侧孢短芽孢杆菌(Brevibacillus laterosporus)作为一种新型生防菌引起了广泛的关注,目前已证明侧孢短芽孢杆菌具有杀虫、杀线、溶磷、降解有机物及抗肿瘤作用,但是对于其抗菌作用研究较少。本试验初步研究了侧孢短芽孢杆菌B8产生胞外抑菌物质对不同细胞壁结构的植物病原真菌的抑菌机理,通过液体深层发酵,无菌发酵液经硫酸铵沉淀、透析等方法得到侧孢短芽孢杆菌B8胞外抑菌物质粗提物。用该粗提物处理辣椒疫霉(Phytophthora capsici)、烟草黑胫病菌(Phytophthora parasitica ***)、黄瓜枯萎病菌(Fusarium oxysporum ***)和立枯丝核菌(Rhizoctonia solani),研究抑菌物质对植物病原真菌的抗生作用、对菌丝的生长和形态的影响、对菌丝细胞膜的透性的影响、对真菌孢子的产生、形态和萌发等方面的影响。研究结果表明,B8产生的胞外抑菌物质均能抑制供试的几种植物病原真菌菌丝的生长,对烟草黑胫病菌、辣椒疫霉、黄瓜枯萎病菌和立枯丝核菌的抑菌率分别为80.25%、74.32%、70.5%和75.91%。用抑菌物质处理后,几种植物病原菌的菌丝形态均发生了明显变化,菌丝末端膨大,菌丝断裂,畸形,菌丝细胞壁消融,菌丝细胞液外渗,最终菌丝消融。B8产生的抑菌物质对供试的植物病原真菌孢子的形成和萌发、菌核的形成和萌发也具有抑制作用。抑菌物质对植物病原菌孢子形成的抑制率随浓度的增加而增加,其中对烟草黑胫病菌游动孢子的产生和萌发的抑制效果最显著。在浓度为0.04mg/L下,对烟草黑胫病菌游动孢子的萌发抑制率为85.06%。B8产生的抑菌物质处理立枯丝核菌发现菌核形成量减少,形成时间增长,萌发时间延迟,将用抑菌物质处理过菌核萌发产生的菌丝重新进行培养发现菌丝生长量明显减小,菌丝畸形。另外,抑菌物质对植物病原真菌的细胞膜有一定程度的损害,处理的菌丝细胞膜的透性变大,能降解菌丝内几丁质,对真菌体内的纤维素酶和蛋白酶活性有一定的影响,明显抑制其菌丝DNA的合成。研究结果表明,抑菌物质具有几丁质酶活性,可破坏植物病原真菌细胞壁中几丁质成分。

关键词：侧孢短芽孢杆菌辣椒疫霉菌烟草黑胫病菌黄瓜枯萎病菌立枯丝核菌抑菌机理

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Molecular Detection of Sclerotinia sclerotiorum Based on cyp51 Gene

Molecular Detection of Sclerotinia sclerotiorum Based on cyp...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Qiao Guanghang Li Xinghong Zhou Ying Zhang Wei Institute of Environment and Plant Protection Beijing Agriculture and Forestry Academy of Sciences

Sterol 14α-demethylase(CYP51),the most widely distributed member of the P450 superfamily,is the key enzyme in sterol biosynthesis ***51 is not only an important model for fundamental P450 structure/function studies,but also an important target protein of cholesterol-lowering agents,antifungal drugs and *** this study,over 20 Ascomycetes cyp51 DNA sequences were compared and a pair of primers(Sscyp51F/Sscyp51R) was designed for detecting ***.6 isolates representing 3 species of Sclerotinia and 10 fungal species of plant pathogens were used to test the specificity of the *** amplification with species-specific(Sscyp51) primers resulted in a product of 340 bp only from isolates of *** detection sensitivity with Sscyp51F/Sscyp51 R primers was 100 pg of genomic *** results showed that cyp51 gene could be a molecular target for detection of *** with the Sscyp51 primers could also be used to detect *** from naturally infected vegetable tissues and infected *** PCR-based method developed in this study provides a rapid and sensitive tool for detection of ***.

关键词： Molecular detection Sclerotinia sclerotiorum cyp51 gene

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