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检索条件"任意字段=中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会"

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Cloning and Sequencing Analysis of Chalcone Synthase Gene from Wheat (Triticum aestivum L.) Infected by Plant Pathogen

Cloning and Sequencing Analysis of Chalcone Synthase Gene fr...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Xue Baoguo Yan Jun Lu Shubing Yang Lirong Quan Xin Plant Protection Institute Henan Academy of Agricultural SciencesChina IPM Key Laboratory in Northern-South of the Ministry of AgricultureHenan Key Laboratory for Control of Crop Diseases and Insect Pests

By screening of expressed genes,the chalcone synthase(CHS) gene was obtained from the wheat(Triticum aestivum L.),infected by plant pathogen Gaeumannomyces graminis *** full-length DNA of the CHS gene was obtained from genomic DNA of Triticum aestivum L,named *** analysis indicated that TaCHS was 2543 bp in full length and contained a promoter region of 1264 bp,an intron of 94 bp,and an opening reading frame(ORF) of 1185 bp encoding a 394 predicted amino acids residues which possessed all the conserved active sites for the CHS function as well as the family *** alignment and phylogenetic analysis of the deduced amino acids revealed that TaCHS shared more than 88%of homology with CHS from other reported plants in *** TaCHS gene from Triticum aestivum was closely related to the gene from Secale cereal,Thinopyrum ponticum and Triticum *** promoter region of TaCHS gene contained fungal elicitor responsive *** also had such typical cis-acting elements as including light responsive elements,phytohormone responsive elements,Myc and Myb binding site,TATA-Box and CAAT-Box.

关键词： Chalcone synthase CHS gene Fungal elicitor Triticum aestivum L.,Gaeumannomyces graminis var.tritici. promoter Myb Myc

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The Plasma Membrane Protein PREN is a Potential Receptor Candidate of Fungal Elicitor PeaT1 which Induces Systemic Acquired Resistance in Tobacco

The Plasma Membrane Protein PREN is a Potential Receptor Can...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Meng Fanlu Xiao Yao Zeng Hongmei Guo Lihua Yang Xiufen Qiu Dewen The State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Science

Protein elicitor PeaT1,which was originally isolated from Alternaria tenuissima,could induce systemic acquired resistance(SAR) to tobacco mosaic virus(TMV) in tobacco *** our study,a 22 kDa tobacco plasma membrane protein which could interact with PeaTl was isolated via Far Western Blot(GenBank;CAA65195.1) and it was designated as PREN(Potential Receptor for an Elicitor with the NAC-domain).The MS analysis for the PREN indicated it belonging to DREPP(developmentally regulated plasma membrane polypeptide) family,which was involved in plant stress responses and signaling ***-Western blot and Co-Immunoprecipitation analysis showed that PREN could associate with PeaTl in vitro under denatured and native conditions,*** fluorescence complementation(BiFC) data indicated that PeaTl could associate with PREN in riving tobacco *** assay result showed that PREN silenced tobacco was more susceptible to TMV than TRV control when treated with PeaT1,providing convincing evidence that PREN could perceive the stimulus from PeaTl which induced SAR in *** downstream signaling pathway needs further study,one thing for certain is that PREN plays an important role as a transducer to perceive the stimulus from PeaTl and mediate the downstream resistance signaling in tobacco plants.

关键词： PeaT1 Systematic acquired resistance Receptor Signaling

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Structure and Function Analysis of Dynein Light Chain Tctex-1 Family Protein MoTctex from the Rice Blast Fungus

Structure and Function Analysis of Dynein Light Chain Tctex-...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Huang Jinguang Li Guorui Zhao Yanxiang Yang Jun Zhao Wensheng Zhang Yan Liu Junfeng Peng Youliang State key Laboratory of Agrobiotechnology China Agricultrual University MOA Key Laboratory of Plant Pathology China Agricultrual University College of Agronomy and Plant Protection Qingdao Agricultural University

Magnaporthe oryzae,the causal agent of rice blast,has been used as a model for investigating the interaction between phytopathogenic fungi and *** genes involved in mycelial growth of *** will contribute to reveal their functions in mycelial growth and to establish the foundation for the future construction of the gene network for regulation of the mycelial growth in ***_ 01005 encodes a protein belonging to dynein fight chain/Tctex-1 family,and the protein was named as *** with the wild type,the delta-MoTctex;mutants grew slower and formed less conidia,indicating that MoTctex plays vital roles in vegetative growth and conidia *** subcellular localization examination demonstrated that the MoTctex-GFP fusion protein was mainly located into cytoplasm of conidia and vegetative *** further understand the molecular bases of the function of MoTctex in ***,the authors have expressed the full-length recombinant protein in Escherichia *** purified protein was crystallized by vapor diffusion method and the crystals were subsequendy used for X-ray diffraction analysis to obtain data at 2 *** crystal structure of the protein will be solved by SAD（Single--wavelength anomalous dispersion） method and the interaction between it and other proteins will be anayzed by GST pull down,yeast two-hybrid and other biophysical methods.

关键词： Magnaporthe oryzae Dynein light chain Tctex-1 Protein crystallization

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Modulation of Virulence by the Second Messenger Cyclic Di-GMP in Dickeya zeae

Modulation of Virulence by the Second Messenger Cyclic Di-GM...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Chen Yufan Lv Mingfa Gu Yanfang Liao Lisheng Liu Shiyin Zhou Jianuan Zhang Lianhui Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University

Dickeya zeae(***) is the causal agent of devastating rice foot rot disease,which is an major concern of the agricultural industry in rice planting countries and *** di-GMP(c-diGMP) is an universal second messenger which regulates a range of important biological function in many bacterial pathogens,including biofilm formation,swarming motility and virulence factors *** activities of diguanylatecyclases(DGCs) and phosphodiesterases(PDEs) control c-diGMP homeostasis in the *** microbes employ large number of genes encoding DGCs and PDEs that are predicted to be part of c-di-GMP signaling ***,the function of cyclic di-GMP in *** and its modulation of virulenceremain largely *** this study,we investigated the influence of c-di-GMP turnover in the virulence of *** *** genes encoding DGCs or PDEs were selected by bioinformatics,and *** these mutants,4 mutants showed a reduction in the production of pectinases,cellulases,proteases and polygalacturonase;2 mutants displayed differences in producing zeamines,comparing with wild *** the pathogenicity assay against rice plant and potato tubers,3 mutants had significantly decreased the pathogenicity in the rice foot rot symptom,while 4 mutants showed little immersion in potato *** of mutants displayed differences in the hypersensitive reaction on tobacco,compared with wild *** findings suggested that the cyclic di-GMP turnover plays a role in the regulation of virulence in *** ECl.

关键词： Cyclic di-GMP Dickeya zeae EC1 Knock-out assay Exoenzyme assay Pathogenicity assay

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Germination and Infectivity of Microconidia in the Rice Blast Fungus Magnaporthe oryzae

Germination and Infectivity of Microconidia in the Rice Blas...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhang Huili Wu Zhongshou Wang Chenfang Li Yang Xu Jinrong State Key Laboratory of Crop Stress Biology for Arid Areas Northwestern A&F University

Many plant pathogenic fungi are ascomycetes that produce more than one type of *** rice blast fungus Magnaporthe oryzae,one of the most destructive diseases of rice and a model for studying fungal—plant interactions,produces both microconidia and ***,previous infection studies exclusively dealt with *** of microconidia has not been observed,and their role in plant infection is not ***,outbreaks of neck blast and wheat blast in fields without foliar diseases indicate that inoculum other than macroconidia may *** we showed that approximately 10%of microconidia could germinate on plant *** colonies derived from germinated microconidia were normal in growth,conidiation,and *** infection assays with rice and barley seedling,microconidia failed to infect intact plants but they could colonize and develop necrotic lesions on wounded leaves and *** also caused disease symptoms on inoculated spikelets without wounding in infection assays with barley and Brachypodium ***,microconidia were detected inside rice plants that developed blast lesions under laboratory or field *** genes encoding hypothetical proteins were identified by RNA-seq analysis to be specifically expressed in microconidia and may be suitable for microconidium *** results demonstrated that microconidiacan germinate and are *** to their size,microconidia produced in planta allow the pathogen to spread readily via the vascular system,which may be an important but neglected factor in the disease cycle and outbreaks of rice blast,wheat blast,and other important diseases caused by Magnaporthe species.

关键词： Wheat blast Pyriculariagrisea Neck blast Head infection

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侧孢短芽孢杆菌B8产生胞外抑菌物质对植物病原真菌抑菌机理的研究

侧孢短芽孢杆菌B8产生胞外抑菌物质对植物病原真菌抑菌机理的研究

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：李悦余惠荣李蔚赵秀香沈阳农业大学植物保护学院

芽孢杆菌在植物病害生物防治中应用广泛。近年来,侧孢短芽孢杆菌(Brevibacillus laterosporus)作为一种新型生防菌引起了广泛的关注,目前已证明侧孢短芽孢杆菌具有杀虫、杀线、溶磷、降解有机物及抗肿瘤作用,但是对于其抗菌作用研究较... 详细信息

芽孢杆菌在植物病害生物防治中应用广泛。近年来,侧孢短芽孢杆菌(Brevibacillus laterosporus)作为一种新型生防菌引起了广泛的关注,目前已证明侧孢短芽孢杆菌具有杀虫、杀线、溶磷、降解有机物及抗肿瘤作用,但是对于其抗菌作用研究较少。本试验初步研究了侧孢短芽孢杆菌B8产生胞外抑菌物质对不同细胞壁结构的植物病原真菌的抑菌机理,通过液体深层发酵,无菌发酵液经硫酸铵沉淀、透析等方法得到侧孢短芽孢杆菌B8胞外抑菌物质粗提物。用该粗提物处理辣椒疫霉(Phytophthora capsici)、烟草黑胫病菌(Phytophthora parasitica ***)、黄瓜枯萎病菌(Fusarium oxysporum ***)和立枯丝核菌(Rhizoctonia solani),研究抑菌物质对植物病原真菌的抗生作用、对菌丝的生长和形态的影响、对菌丝细胞膜的透性的影响、对真菌孢子的产生、形态和萌发等方面的影响。研究结果表明,B8产生的胞外抑菌物质均能抑制供试的几种植物病原真菌菌丝的生长,对烟草黑胫病菌、辣椒疫霉、黄瓜枯萎病菌和立枯丝核菌的抑菌率分别为80.25%、74.32%、70.5%和75.91%。用抑菌物质处理后,几种植物病原菌的菌丝形态均发生了明显变化,菌丝末端膨大,菌丝断裂,畸形,菌丝细胞壁消融,菌丝细胞液外渗,最终菌丝消融。B8产生的抑菌物质对供试的植物病原真菌孢子的形成和萌发、菌核的形成和萌发也具有抑制作用。抑菌物质对植物病原菌孢子形成的抑制率随浓度的增加而增加,其中对烟草黑胫病菌游动孢子的产生和萌发的抑制效果最显著。在浓度为0.04mg/L下,对烟草黑胫病菌游动孢子的萌发抑制率为85.06%。B8产生的抑菌物质处理立枯丝核菌发现菌核形成量减少,形成时间增长,萌发时间延迟,将用抑菌物质处理过菌核萌发产生的菌丝重新进行培养发现菌丝生长量明显减小,菌丝畸形。另外,抑菌物质对植物病原真菌的细胞膜有一定程度的损害,处理的菌丝细胞膜的透性变大,能降解菌丝内几丁质,对真菌体内的纤维素酶和蛋白酶活性有一定的影响,明显抑制其菌丝DNA的合成。研究结果表明,抑菌物质具有几丁质酶活性,可破坏植物病原真菌细胞壁中几丁质成分。

关键词：侧孢短芽孢杆菌辣椒疫霉菌烟草黑胫病菌黄瓜枯萎病菌立枯丝核菌抑菌机理

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Expression of the Single-stranded DNA-binding Protein (Ssb) in Transgenic Tobacco Enhanced Pathogen Defense

Expression of the Single-stranded DNA-binding Protein (Ssb) ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Sun Yujing Ma Wenxiu Cai Lulu Liu Liang Zou Lifang Chen Gongyou School of Agriculture and Biology Shanghai JiaoTong University

Previously we reported that a highly-conserved single-stranded DNA-binding protein（Ssb） of Xanthomonas species,secreted by the type-Ⅲ secretion system（T3SS）,acts as harpin-like proteins to elicit hypersensitive response（HR） in nonhost *** this study,we cloned ssb gene from *** ***（Xoc） that causes bacterial leaf streak in rice,into tobacco（***）via Agrobacterium-mediated *** ssb transgenic tobacco showed smaller phenotype in whole life cycle,including stems,***,HRs occurred earlier in ssbtransgenic tobacco than the wild-type and the empty vector（EV） plants when Ssb and Hpal（a harpin protein of Xoc）were *** micro-HR,and more callose deposition and reactive oxygen spcies（ROS） accumulation were also observed in *** addition,ssbtransgenic tobacco plants were more resistant to Pseudomonas syringae *** DC3000 infection,comparing to the wild-type and EV ***,the expression of HR-makered genes（HIN1 and HSR203J） and pathogenesis-related protein genes（PR1a,PR1 b and SGT1） were significantly higher in ssbtobacco than in the wildtype and EV *** findings displayed that ssb play an important role in nonspecific resistance to pathogens,and provide an insight into researching its molecular mechanism of interaction with host plant.

关键词： Xanthomonas Transgenic tobaccos Hypersensitive response(HR)

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Spatial Variation of Sensitivity to Azoxystrobin in the Phytophthora infestans Populations in China

Spatial Variation of Sensitivity to Azoxystrobin in the Phyt...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Qin Chunfang Chen Fengping Zhu Wen Zhan Jiasui Key Laboratory of Plant Virology of Fujian Province Institute of Plant VirologyFujian Agriculture and Forestay

Potato late blight（Solanum tuberosun L.） caused by Phytophthora infestans（Mont）de Bary,is an economically important crop disease in the *** is a fungicide commonly used in agriculture to control late *** is QoI fungicide which blocks electron transfer and impairs ATP synthesis in *** is well known that single point mutations in target sites of pathogens can lead to Azoxystrobin *** this study,we analyzed the spatial distribution of Azoxystrobin by *** sensitivity in the 140 isolates of Phytophthora *** isolates were collected from seven locations including one each in Gansu,Yunnan,Guangxi,Guizhou and Ningxia and two in Fujian.20 genetically distinct isolates from each population were *** to azoxystrobin in each isolate was measured by relative colony size in the presence and absence of the fungicide with a Common Garden experiment under four *** sensitivity in the 140 isolates ranged from 0.36 to 0.75 and displayed a continuous,unimodal distribution,suggesting other minor genes may modify the sensitivity of *** to the *** difference in the sensitivity was found among populations by least significant difference（LSD） analysis（P <0.001） and isolates（P<0.001）.Population differentiation for azoxystrobin sensitivity among the seven populations(Q =0.19) was higher than the corresponding population differentiation in SSRs(F =0.12),consistent with selection for local adaption in azoxystrobin sensitivity.

关键词： Phytophthora infestans Azoxystrobin Sensitivity Spatial Population Structure Common Garden design Experimental evolution

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SSR Linkage Map Construction and QTL Analysis for the Resistance to Rice Black-streaked Dwarf Disease in Rice

SSR Linkage Map Construction and QTL Analysis for the Resist...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Du Linlin Zhou Tong Lan Ying Fan Yongjian Zhou Yijun Institute of Plant Protection Jiangsu Academy of Agricultural SciencesJiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Disease

Rice black-streaked dwarf disease,caused by Rice black-streaked dwarf virus（RBSDV）transmitted by the small brown planthopper（SBPH,Laodephax striatellus Fallen）,is a serious threat to rice *** our study,an RBSDV-resistant variety Tetep from Vietnam was screened by the field test and verified by the artificial inoculation *** result of SBPH and RBSDV resistance evaluation revealed rice black-streaked dwarf disease resistance of Tetep derived from resistance to RBSDV rather than *** analysis and QTL mapping were carried out using F populations derived from the cross between Huaidao No.5 and Tetep.A genetic linkage map was constructed containing 127 SSR markers,which covered 2179.6 cM of the rice genome with an average distance of 17.16 cM between *** analysis of the inheritance of resistance to RBSDV in Tetep was controlled by quantitative trait locus（QTL）.Two new QTL were identified on chromosomes 3 and 11,which explained about 17.5%and12.4%of the phenotypic variance with LOD scores of 4.07 and 2.21,*** addition,resistance to RBSDV conferred by qRBSDV-3 and qRBSDV-11 was predominantly controlled by additive gene action and both derived from the resistant parent Tetep.

关键词： QTL SSR Linkage Map Construction and QTL Analysis for the Resistance to Rice Black-streaked Dwarf Disease in Rice

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Study on the Sporulation and Toxin for Corynespora *** Kiwi Brown spot

Study on the Sporulation and Toxin for Corynespora *** Kiwi ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：崔永亮 Wen X.W. 龚国淑 Xu J. Zhou Y. Zheng X.Q. Qi X.B. Lv Zh. Liu N. Agricultural college Sichuan Agricultural University Sichuan Provincial Academy of Natural Resource and Sciences

Kiwi brown spot is one major disease in Sichuan kiwifruit production areas in recent years,which occurs in mid-to-late of June and widely spreads in mid-to-late of July,and the diseased leaf rate nearly reaches 50%~ 80%in most of production orchards in *** this paper,the sporulation condition of the causal agent Corynespora *** optimized,and the pathogenesis of synthesized toxin was *** test of eight pathogenic isolates from the infected leaves in different disease sites showed that strain CQ2-0 from Qionglai had the strongest *** of the sporulation condition for three strong pathogenic strains demonstrated that nutritional deficiency positively stimulate sporulation,and the optimized condition was that pathogen was cultured on PDA medium with initial pH 4 at 25℃ under continuous *** of toxin production condition and toxicity of CQ2-0revealed that the fungus produced toxin in the modified Frise No.3 medium at 25 ℃ with light on ***,typical brown spot symptoms appeared when inoculated with mycelial plugs,spore suspension,and crude toxin of the *** was concluded that toxin secreted by Corynespora *** the main pathogenic factor.

关键词： Study on the Sporulation and Toxin for Corynespora sp.of Kiwi Brown spot

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