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First Report of the Occurrence of Geminivirus in Hubei

First Report of the Occurrence of Geminivirus in Hubei

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhang Songbai Du Zhenguo Wang Zhen He Zifu College of Agriculture Yangtze University Plant Protection Research Institute GDAAS

In September 2013,tall morning glory（Ipomoea purpurea） plants showing vein yellowing and leaf curl symptoms typical of a begomovirus infection（figure） were observed in Jingzhou,Hubei Province,*** nucleic acids were extracted from a symptomatic plant using CTAB *** circle amplification（RCA） was conducted using TempliPhikit（GE Healthcare,USA） to recover the genome of a putative *** of the RCA product with Pst I yielded a ~ 2.8 kbDNA fragment suggestive of a monomerized begomoviral *** fragment was cloned and sequenced and the sequence was deposited in GenBank under accession ***.1.0（Species Demarcation Tool） analysis revealed that the putative begomovirus showed 98.5%and 92.0%nucleotide sequence identity with sweet potato leaf curl Georgia virus（SPLCGV）-[China:Hebei:2011]（JX448368） and SPLCGV-[US:Geo:16]（AF326775）,*** virus contains six ORFs which show 98%~ 100%amino acid sequence identity with AV1,AV2 and AC1-AC4 of SPLCGV-[China:Hebei:2011],***（genus Begomovirus,family Geminiviridae） has been reported to infect sweet potato（Ipomoea batatas） in USA,India and *** our knowledge,this is first report of a geminivirus in Hubei,a Province of Central *** the finding of SPLCGV in sweet potato may be a result of vegetative propagation this crop,the detection of SPLCGV in tall morning glory clearly,an annual plant,shows that this virus is transmissible and is spreading in *** suggests that SPLCGV may pose a greater threat to sweet potato cultivation in China than previously thought.

关键词： Geminivirus Ipomoea purpurea Sweet potato leaf curl virus

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Functional Analysis of the FgCDC14 Gene in the Wheat Scab Fungus Fusarium graminearum

Functional Analysis of the FgCDC14 Gene in the Wheat Scab Fu...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Li Chaohui Zhang Shijie Zhang Hongchang Mark Hall Xu Jinrong State Key Laboratory of Crop Stress Biology for Arid Areas Northwestern A&F University Department of Biochemistry Purdue University

Mitosis and cytokinesis are essential cellular processes for cell division and proliferation in eukaryotic *** genes important for cell cycle have been well characterized in Saccharomyces cerevisiae and Schizosaccharomyces pombe,including CDC14 that encodes a member of the dual specificity phosphatase family highly selective for serine-proline phosphorylation sites generated by cyclin-dependent ***,whereas CDC14 is an essential gene for regulating multiple events during anaphase such as mitotic exit in ***,its ortholog is not essential in *** although it is important for coordinating nuclear division with ***14 orthologs are well conserved in filamentous fungi,but none of them have been functionally *** this study,we aimed to determine the functions of CDC14 in growth,development,and pathogenesis in Fusarium graminearum,the causal agent of wheat and barley head *** of FgCDC14 is not lethal in *** but the Fgcdc14 deletion mutant was significantly reduced in vegetative growth,conidiation,and ascospore *** self-cross assays,the Fgcdc14 deletion mutants produced morphologically normal perithecia that contained aborted ascogenous *** plant infection assays,the Fgcdc14 mutant was *** conidia had abnormal morphology,fewer septa,and more nuclei per compartment compared to the wild-type *** in mutant hyphae also was reduced and *** eonidium compartments contained multiple nuclei,indicating that the Fgcdc14 mutant was defective in the coordination between nuclear division and ***,foot cells of macroconidia were able to exit Gl-arrest and extend and function as conidiogenous cells in the Fgcdcl4 mutant,resulting in the production of inter-connected *** conidia germinated when they were still on the *** germination mainly happened on the foot cell of the conidia but also could be observed on t

关键词： Phosphatase Mitosis Cytokinesis Septation CDC14

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Study on Virus Elimination of Apple Potted Plants by Thermotherapy

Study on Virus Elimination of Apple Potted Plants by Thermot...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Hu Guojun Zhang Zunping Dong Yafeng Fan Xudong Ren Fang Zhu Hongjuan National Center for Eliminating Viruses from Deciduous Fruit Tree Research Institute of PomologyChinese Academy of Agriculture Sciences

In this study,four kinds of potted apple varieties infected apple chlorotic leaf spot virus（ACLSV）,apple stem pitting virus（ASPV）,apple stem grooving virus（ASGV）,apple mosaic virus（ApMV） and apple scar skin viroid（ASSVd） were used as original *** effect of the size of shoot（1 cm and 2 ~ 3cm） cut after constant thermotherapy（37 ± 1） ℃ on graft survival rate and efficiency of virus elimination was compared,and the elimination rate of different variety and different virus was *** results showed that the size of shoot was significant to the graft survival rate and virus elimination *** rate of 1 cm shoos was half of 2 cm shoot,but its virus elimination rate was increased by 27.6%,comparing with 2 ~ 3cm *** virus elimination of different variety existed *** the four varieties,the average virus elimination rate of Zhongqiuwang was the highest,44.8%,and Dahongrong with 11.8%was relatively lower;the elimination rate of different viruses was different,the detection results of treated potted apples in 2013 showed that ASPV was easy to be eliminated,with the average elimination rate 90.9%,ACLSV,ASSVd and ApMV was in the middle,with the average elimination rate 78.7%,70.6%and 66.7%,respectively,and the average elimination rate of ASGV with 59.2%was the *** this research,we acquired twenty-eight virus-free varieties from four apple varieties,Dahongrong,Qiyuetianxian,Xinnonghuang and Zhongqiuwang.

关键词： Study on Virus Elimination of Apple Potted Plants by Thermotherapy

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Screening for New Fungicides of Ginseng Black Spot(Alternaria panax)

Screening for New Fungicides of Ginseng Black Spot(Alternari...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Li Xinyue Zhou Rujun Fu Junfan Plant Protection College Shenyang Agricultural University

Ginseng（Panax ginseng ***） as a valuable Chinese herbal medicine,is a perennial herb of *** is put as a superior medicine by the earliest pharmacy book of China "Sheng Nong's herbal classic".With the efficacy of nourishing vitality,calm the nerves and gain intelligence,regulate fortify the five organs,dredge the meridians,delay fading and so on,known as the " king of herbs " in the *** in recent years,the yield of ginseng is not very high,because the cultivation and growth of ginseng often suffers the threat of disease,thus affected the yield of *** has a wide variety of diseases,there are more than 20 kinds in China,in which ginseng black spot occurs most commonly in ginseng aboveground,mainly damaging the leaves,stems of fruits as well as the *** incidence of disease is 20%~ 30%and can reach 100%in severe,it causes the early defoliation,plants withered in advance,no fruits and ginseng roots outputs will cut 30%0%.The fungicides against this disease mainly were Carbendazim,Amobam,Polyoxin,Chlorothalonil,Mancozeb,Iprodione and so *** to the field crops,the variety of pesticides are used obsolete,large doses and high density,but with little effect in ginseng black spot In order to update the type of fungicides against ***,improve the prevention effect,prevent or slow down the generation of pathogens drug-resistant,we selected nine pesticides indoors against Alternaria panax to do efficacy *** test used the growth rate method measuring the nine kinds of fungicide on the virulence of *** results showed that there were large differences between nine kinds of fungicide to affect *** best inhibitory effect was 10%difenoconazole WG,inhibitory rate was 87.89%,50%Kaiser 1000 times,60%Baitai WG 1000 times,70%metiram WG2000 times was followed,inhibition rate was 76.99%,74.62%and 70.79%*** *** the worst with the inhibitory effect of 50%.

关键词： Alternaria panax Fungicide Toxicity test

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PCR-based Assay for the Detection of Xanthomonas campestris *** Causing mango Bacterial Black spot

PCR-based Assay for the Detection of Xanthomonas campestris ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Qi Yanxiang Pu Jinji Zhang He Xie Yixian Zhang Xin Lu Ying Yu Qunfang Zhang Huiqiang Environment and Plant Protection Institute Chinese Academy of Tropical Agricultural Sciences

Mango(Mangifera indica L.) is cultivated throughout the tropics as well as in subtropical areas and suffers from a number of diseases caused by bacteria,fungi and other *** no efficient chemical control is available,mango bacterial black spot(MBBS;synonym mango bacterial canker) caused by Xanthomonas campestris ***(Xcmi;synonym *** ***) is a recurring limiting factor for mango industries in the Old *** movement of infected,asymptomatic leaf,branch and fruit is a major means of pathogen dispersal.A reliable and sensitive diagnostic procedure is necessary for the safe movement of mango planting material.A set of primers based on the partial sequence of the hrpB gene was designed to develop a PCR assay for this *** pairs(XcmiHF and XcmiHR),which amplified a single specific band of 321 bp,were tested for specificity and sensitivity in detection DNA and cell from *** was positive only with genomic DNA from 12 Xcmi strains,no PCR products were observed with DNA from 10 other Xanthomonads and 7 other bacterial *** sensitivity of detection was 2.4 pg of plasmid DNA,and1.8 ×10～4CFU/mL *** primers also worked well for pathogen detection in direct PCR assays of Xcmi colonies grown on liquid medium and in PCR assays of total DNA from leaf,branch and fruit *** assays proved to be relatively sensitive and could become very useful in detecting Xcmi in mango planting material.

关键词： molecular diagnosis hrpB PCR phytopathogen Xanthomonas campestris pv.mangiferaeindicae

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Preliminary Screening of Bacteria against Soybean Cyst Nematode in the Northeast of China

Preliminary Screening of Bacteria against Soybean Cyst Nemat...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Liu Dan Zhao Dan Wu Jubao Wang Yuanyuan Zhu Xiaofeng Chen Lijie Duan Yuxi Nematology Institute of Northern China College Plant ProtectionShenyang Agricultural University

The soybean cyst nematode(SCN,Heterodera glycines) is one of most important pests on soybean,which can cause major losses in soybean production *** chemical control is neither continually effective nor environmentally friendly,many researches have been focused on eliciting the plants defense *** species of bacteria had been reported to induce soybean plant resistance against *** the research,810 species of bacterium cultures that conserved by Nematology Institute of Northern China were coated on soybean seeds(cultivar Liaodou 15).And all coated seeds were sowed in SCN high-infected *** 35 days,roots of soybean seedlings were pulled out to assess the infection *** that made roots have lower infection level were *** preliminary screening,soybean plants were well induced resistance against SCN by 27 selected *** bacteria were indetifited by morphology and 16 s rDNA polymerase chain reaction,which involved in 10 genera,including Bacillus spp.,Pseudomonas spp.,Rhodococcus sp.,Microbacterium sp.,Paenibacillus sp.,Leuconostoc sp.,Rahnella sp.,Sporosarcina sp.,Yersinia *** Stenotrophomonas sp..Most bacteria were from two genera,Bacillus and Pseudomonas,which have been reported as biocontrol bacteria many *** effective bacteria are potential to control SCN in an environmentally friendly way,and the mechanism of them is worth of research.

关键词： Soybean cyst nematode Bacterium Induced resistance Screening

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Establish ATMT Mutant Library of Cecrospora zeaemaydis

Establish ATMT Mutant Library of Cecrospora zeaemaydis

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Lu Yuanyuan Xiao Shuqin He Ruihong Zhao Likun Xue Chunsheng Department of Plant Protection Shenyang Agriculture University

Gray leaf spot of corn is an important disease caused by Cecrospora *** present,research on *** mainly focus on the biological characteristics,resistance heredity,evaluation of varieties resources and pathogenesis factors,but less in pathogenesis *** study aims to establish the mutant library by ATMT to based on research the pathogenesis mechanism of *** the basis of hygromycin and green fluorescent protein gene,designs primers to amplify the marker gene,then connected to the backbone vector pPZP100,final constructed the vector with hygromycin and green fluorescent protein gene as a *** the condition of ATMT and establish ATMT mutant library of *** to get 660 *** factors are as follow;spores of *** cultured on V were collected 5d after cultivation and prepared for spore suspension at 10 /mL,then germination 12 h and cultured together with AGL-1(OD =0.5) for 2d in 25℃.Hygromycin B tolerance of *** was 150mg/*** efficiency of transformation is 25/10～5 per milliliter.28 transformants are confirmed by primers form hygromycin gene,which transferred to PDA select medium total 5 time,1300 bp fragment showed that T-DNA had successfully inserted to genome DNA of *** show green fluorescent via the micro-observation.23 strains were random selected through morphological observation,the result show that mutant strains and wild-type strain have significant different with colonial morphology *** side and back side colour of the wild-type strains are mainly black,the transformants strains of positive side are mainly white and red,back side are green *** analysis showed that most transformants strains infected plant,but the pathogenicity obviously weaken.

关键词： Cecrospora zeaemaydis ATMT Mutant library

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Jasmonate Signalling and the Two Layers of Plant Immunity-Lessons from Grapevine Cells

Jasmonate Signalling and the Two Layers of Plant Immunity-Le...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Chang Xiaoli Mitsunori Seo Yumiko Takebayashi Yuji Kamiya Michael Riemann Gong Guoshu Peter Nick Department of Plant Pathology Agricultural CollegeSichuan Agricultural University RIKEN Plant Science Center Molecular Cell Biology Botanical InstituteKarlsruhe Institute of Technology

To protect their tissues against various stimuli,plants have evolved two modes of immunity,pathogen-associated molecular pattern(PAMP) triggered immunity(PTI) and effector-triggered immunity(ETI),which extensively share signalling machinery and discriminate by the occurrence of hypersensitive cell *** hormones act an important layer of plant immunity with jasmonic acid(JA) and salicylic acid(SA) as prime *** our previous work,we have mapped defence related responses in two grapevine cell lines derived from genotypes that are either susceptible to important pathogens(cv.'Pinot Noir') or,in consequence of coevolution with those pathogens have acquired resistance correlated with the ability for a vigorous hypersensitive response(***).However,timing and magnitude of oxidative burst and the synthesis of toxic phytoalexins *** the current work,we use this experimental system to dissect the role of the JA- and the SA-pathway for *** observe on the one hand that,in ***,exogenous MeJA can amplify Harpin-induced cell death and induce StSy transcripts as well as the cytotoxic products of StSy,resveratrol and *** the other hand,Harpin does not produce any accumulation of JA,nor of *** contrast,flg22 can induce StSy transcripts as well,but not the accumulation of stilbenes nor any cell ***,flg22 causes a strong,but transient induction of JA and *** places(early and regulated) induction of JA into the PTI pathway,whereas continuous treatment with MeJA seems to be related with ETI-like cell death.

关键词： Effector-triggered immunity(ETI) Jasmonic acid(JA) PAMP-triggered immunity(PTI) Salicylic acid(SA) Vitis

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The Proteomics of Phytophthora infestans Effector Protein NPP1

The Proteomics of Phytophthora infestans Effector Protein NP...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：周晓罡 Ding Yumei Yao Chunxin Tao Nan Sun Maolin Biotechnology and Germplasm Resources Institute Yunnan Academy of Agricultural Sciences Yunnan Provincial Key Lab of Agricultural Biotechnology Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation Ministry of Agriculture

The secreted proteins of Phytophthora infestans were separated by two-dimensional electrophoresis with immobilized pH（4 ,non-inear） gradients as the first dimension and SDS-PAGE as the *** silver-stained proteins spots were analyzed by using PDQuest 7.1.0 software.850 detectable protein spots were detected on the *** were 257 proteins in the 0 ~ 25 kDa Molecular weight,448 proteins in the 25 0 kDa,128 proteins in the 50 5 kDa,and 17 proteins more than75 *** isoelectric point of 317 proteins was 4 ,363 proteins was 5 ,and 170 proteins was6 .By MALDI-TOF mass spectrometry analysis and Mascot database searching,13 protein spots were *** those proteins,NPP1（Necrosis-inducing Phytophthora） protein of *** were *** NPP1 published protein sequences of *** was analyzed,the signal peptide was predicted by SignalP v3.0 and PSORT,the transmembrane helix was predicted by TMHMM-2.0 and THUMBUP,the big-PI Predictor was used to predicted GPI-anchoring site,and subcellular protein location distribution was analyzed by TargetP *** resultes showed that the NPP1 protein was secreted protein with signal peptide,the signal peptide was composed by 19 amino acid,the NPP1's Molecular weight was 25518.2 Da,and the isoelectric point was 5.27.

关键词： Phytophthora infestans Proteomics Effector protein

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Identification of Resistance to Early Leaf Spot on Major Peanut Cultivars in Liaoning Province,China

Identification of Resistance to Early Leaf Spot on Major Pea...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Cui Jianchao Wang Zhen Zhou Rujun Fu Junfan College of Plant Protection Shenyang Agricultural University

Peanut（Arachis hypogeal L.） is an important oil crop because of its high protein and oil *** the increase of cultivated area,China has become the second largest peanut planted country in the *** leaf spot also becomes one of the important factors limiting peanut productivity in China,particularly in Liaoning *** leaf spot caused by Cercospora arachiddicola *** chlorotic spots appeared initially on leaflets in 10 days after infection,and then brown to dark brown,sporulating lesions develop into mature in 15 days,the spot surrounded by yellow *** foliar applications of fungicides,the disease may cause up to 100%defoliation prior to harvest and losses in excess of 50%of potential *** effective chemical control methods are available,their applications are limited because of the pesticide residues and possible existence of fungicide-tolerant strains of the *** plant resistance is thus a valuable tool in a disease management *** main objective of this research was to identify the major cultivars of peanut which were resistance to the leaf spot disease in Liaoning province and to provide the theoretical basis for resistance breeding and prevention field diseases.16 peanut cultivars were evaluated for disease resistance to *** in the cropping field of four consecutive years in Fuxin City,Liaoning Province,*** experiment was carried out under natural condition in 2013.20 days before harvest,the disease was evaluated to the resistance of the *** disease on individual leaves of each peanut plant was classified according to the following ratings:0 = no leaf spot;1 = very few lesions on the leaves,none on the upper canopy;2 = very few lesion on the middle leaves;3 = lesions numerous and very evident on lower and middle leaves,some lesions on the upper canopy;4 = leaves covered with lesions and plant defoliated *** to the disease assessment criterion,we calculat

关键词： Peanut cultivars Early leaf spot disease Relative resistance index Resistance identification

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