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检索条件"任意字段=中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会"

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Colonization of a Green Fluorescent Protein Tagged Biocontrol Strain B96-IIG13 in Asparagus officinalis

Colonization of a Green Fluorescent Protein Tagged Biocontro...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Hao Bianqing Ma Liping Qiao Xiongwu Institute for Agro-Product Quality Safety and TestingShanxi Academy of Agricultural Sciences

Bacillus subtilis B96-Ⅱ is a broad-spectrum biological control strain isolated from compost *** could effectively suppress the soil-borne fungal diseases of *** B96-ⅡG13 was obtained by tagging B96-Ⅱ with the green fluorescent protein gene(gfp) for detecting colonization in *** strain B96-ⅡG13 exhibited brightly green fluorescence under a fluorescence *** gfp was stable with no apparent adverse affects on the growth of wild-type *** plants were planted in the soil inoculated with the B96-Ⅱ*** counting the number of colony forming units(cfu) of strain B96-ⅡG13 on selective plate culture and fluorescence microscope observation,the colonization and translocation capability of B96-ⅡG13 in asparagus plants were *** results showed B96-IIG13 could colonize in the asparagus root system.B96-ⅡG13 could be found from root tip to root base of the asparagus plant,and the population density showed an increasing trend from root tip to root *** addition,B96-ⅡG13 could also be found decreased from stem base to stem top;the most population density of B96-ⅡG13 appeared in the stem base 15 days after planting.B96-ⅡG13 could still maintain certain population density from root tip to stem top 45 days after ***,successful colonization of *** B96-ⅡG13 in asparagus root system and translocation from root to stem could be concluded.

关键词： Asparagus officinalis Bacillus subtilis Colonization Green fluorescent protein

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PCR-based Assay for the Detection of Xanthomonas campestris *** Causing mango Bacterial Black spot

PCR-based Assay for the Detection of Xanthomonas campestris ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Qi Yanxiang Pu Jinji Zhang He Xie Yixian Zhang Xin Lu Ying Yu Qunfang Zhang Huiqiang Environment and Plant Protection Institute Chinese Academy of Tropical Agricultural Sciences

Mango(Mangifera indica L.) is cultivated throughout the tropics as well as in subtropical areas and suffers from a number of diseases caused by bacteria,fungi and other *** no efficient chemical control is available,mango bacterial black spot(MBBS;synonym mango bacterial canker) caused by Xanthomonas campestris ***(Xcmi;synonym *** ***) is a recurring limiting factor for mango industries in the Old *** movement of infected,asymptomatic leaf,branch and fruit is a major means of pathogen dispersal.A reliable and sensitive diagnostic procedure is necessary for the safe movement of mango planting material.A set of primers based on the partial sequence of the hrpB gene was designed to develop a PCR assay for this *** pairs(XcmiHF and XcmiHR),which amplified a single specific band of 321 bp,were tested for specificity and sensitivity in detection DNA and cell from *** was positive only with genomic DNA from 12 Xcmi strains,no PCR products were observed with DNA from 10 other Xanthomonads and 7 other bacterial *** sensitivity of detection was 2.4 pg of plasmid DNA,and1.8 ×10～4CFU/mL *** primers also worked well for pathogen detection in direct PCR assays of Xcmi colonies grown on liquid medium and in PCR assays of total DNA from leaf,branch and fruit *** assays proved to be relatively sensitive and could become very useful in detecting Xcmi in mango planting material.

关键词： molecular diagnosis hrpB PCR phytopathogen Xanthomonas campestris pv.mangiferaeindicae

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Functional Analysis of the FgCDC14 Gene in the Wheat Scab Fungus Fusarium graminearum

Functional Analysis of the FgCDC14 Gene in the Wheat Scab Fu...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Li Chaohui Zhang Shijie Zhang Hongchang Mark Hall Xu Jinrong State Key Laboratory of Crop Stress Biology for Arid Areas Northwestern A&F University Department of Biochemistry Purdue University

Mitosis and cytokinesis are essential cellular processes for cell division and proliferation in eukaryotic *** genes important for cell cycle have been well characterized in Saccharomyces cerevisiae and Schizosaccharomyces pombe,including CDC14 that encodes a member of the dual specificity phosphatase family highly selective for serine-proline phosphorylation sites generated by cyclin-dependent ***,whereas CDC14 is an essential gene for regulating multiple events during anaphase such as mitotic exit in ***,its ortholog is not essential in *** although it is important for coordinating nuclear division with ***14 orthologs are well conserved in filamentous fungi,but none of them have been functionally *** this study,we aimed to determine the functions of CDC14 in growth,development,and pathogenesis in Fusarium graminearum,the causal agent of wheat and barley head *** of FgCDC14 is not lethal in *** but the Fgcdc14 deletion mutant was significantly reduced in vegetative growth,conidiation,and ascospore *** self-cross assays,the Fgcdc14 deletion mutants produced morphologically normal perithecia that contained aborted ascogenous *** plant infection assays,the Fgcdc14 mutant was *** conidia had abnormal morphology,fewer septa,and more nuclei per compartment compared to the wild-type *** in mutant hyphae also was reduced and *** eonidium compartments contained multiple nuclei,indicating that the Fgcdc14 mutant was defective in the coordination between nuclear division and ***,foot cells of macroconidia were able to exit Gl-arrest and extend and function as conidiogenous cells in the Fgcdcl4 mutant,resulting in the production of inter-connected *** conidia germinated when they were still on the *** germination mainly happened on the foot cell of the conidia but also could be observed on t

关键词： Phosphatase Mitosis Cytokinesis Septation CDC14

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Preliminary Screening of Bacteria against Soybean Cyst Nematode in the Northeast of China

Preliminary Screening of Bacteria against Soybean Cyst Nemat...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Liu Dan Zhao Dan Wu Jubao Wang Yuanyuan Zhu Xiaofeng Chen Lijie Duan Yuxi Nematology Institute of Northern China College Plant ProtectionShenyang Agricultural University

The soybean cyst nematode(SCN,Heterodera glycines) is one of most important pests on soybean,which can cause major losses in soybean production *** chemical control is neither continually effective nor environmentally friendly,many researches have been focused on eliciting the plants defense *** species of bacteria had been reported to induce soybean plant resistance against *** the research,810 species of bacterium cultures that conserved by Nematology Institute of Northern China were coated on soybean seeds(cultivar Liaodou 15).And all coated seeds were sowed in SCN high-infected *** 35 days,roots of soybean seedlings were pulled out to assess the infection *** that made roots have lower infection level were *** preliminary screening,soybean plants were well induced resistance against SCN by 27 selected *** bacteria were indetifited by morphology and 16 s rDNA polymerase chain reaction,which involved in 10 genera,including Bacillus spp.,Pseudomonas spp.,Rhodococcus sp.,Microbacterium sp.,Paenibacillus sp.,Leuconostoc sp.,Rahnella sp.,Sporosarcina sp.,Yersinia *** Stenotrophomonas sp..Most bacteria were from two genera,Bacillus and Pseudomonas,which have been reported as biocontrol bacteria many *** effective bacteria are potential to control SCN in an environmentally friendly way,and the mechanism of them is worth of research.

关键词： Soybean cyst nematode Bacterium Induced resistance Screening

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Distribution and Pathogen Identification of Wheat Soil-borne Virus Disease in Shandong Province

Distribution and Pathogen Identification of Wheat Soil-borne...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Wu Bin Xin Xiangqi Wang Shengji Zhang Mei Zhao Jiuhua Institute of Plant Protection Shangdong Academy of Agricultural Sciences

Wheat soil-borne virus disease is one of the most important threats to winter *** pathogen is Wheat yellow mosaic virus（WYMV） and Chinese wheat mosaic virus（CWMV）,which spread by Pofymyxa graminis L in a persistent *** order to investigate the distribution of these virus,117 wheat samples were collected from 38 areas and detected by using ELISA and RT-PCR in *** analysed the coat protein（CP） gene sequence of these two virus,then the phylogenetic tree was *** show that,WYMV was detected in samples from Junan,linshu,Tancheng in Linyi,Boshan in Zibo,Tengzhou in Zaozhuang and Juxian in Rizhao,with a positive rate of 30.77%;CWMV was detected in samples from Rongcheng in Weihai,Pingyuan in Dezhou and Yishui in Linyi,with a positive rate of 21.37%.Both WYMV and CWMV are detected in samples from Fushan,Wendeng in Yantai,and Jiaozhou in *** homology of WYMV isolates CP gene is 97.3%9.1%.Isolate from Junan and Juxian had a close phylogenetic relationship with isolate from Sichuan（AJ240050）,isolate from Tengzhou had a close relationship with Jiangsu isolate（FR828089）.It has a 95.2%~ 98.2%homology in CWMV CP gene,which were clustered into two *** group include isolates from Pingyuan,Wendeng and Yantai,the other include isolates from Yishui,Rongcheng and *** results shows that there were genetic diversity in CP gene of WYMV and CWMV.

关键词： Wheat Soil-borne Virus Disease Wheat yellow mosaic virus(WYMV) Chinese wheat mosaic virus(CWMV) Sequence Analysis

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Jasmonate Signalling and the Two Layers of Plant Immunity-Lessons from Grapevine Cells

Jasmonate Signalling and the Two Layers of Plant Immunity-Le...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Chang Xiaoli Mitsunori Seo Yumiko Takebayashi Yuji Kamiya Michael Riemann Gong Guoshu Peter Nick Department of Plant Pathology Agricultural CollegeSichuan Agricultural University RIKEN Plant Science Center Molecular Cell Biology Botanical InstituteKarlsruhe Institute of Technology

To protect their tissues against various stimuli,plants have evolved two modes of immunity,pathogen-associated molecular pattern(PAMP) triggered immunity(PTI) and effector-triggered immunity(ETI),which extensively share signalling machinery and discriminate by the occurrence of hypersensitive cell *** hormones act an important layer of plant immunity with jasmonic acid(JA) and salicylic acid(SA) as prime *** our previous work,we have mapped defence related responses in two grapevine cell lines derived from genotypes that are either susceptible to important pathogens(cv.'Pinot Noir') or,in consequence of coevolution with those pathogens have acquired resistance correlated with the ability for a vigorous hypersensitive response(***).However,timing and magnitude of oxidative burst and the synthesis of toxic phytoalexins *** the current work,we use this experimental system to dissect the role of the JA- and the SA-pathway for *** observe on the one hand that,in ***,exogenous MeJA can amplify Harpin-induced cell death and induce StSy transcripts as well as the cytotoxic products of StSy,resveratrol and *** the other hand,Harpin does not produce any accumulation of JA,nor of *** contrast,flg22 can induce StSy transcripts as well,but not the accumulation of stilbenes nor any cell ***,flg22 causes a strong,but transient induction of JA and *** places(early and regulated) induction of JA into the PTI pathway,whereas continuous treatment with MeJA seems to be related with ETI-like cell death.

关键词： Effector-triggered immunity(ETI) Jasmonic acid(JA) PAMP-triggered immunity(PTI) Salicylic acid(SA) Vitis

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The Application of Renilla luciferase in the Research on Translational Recoding in Tobacco Bushy Top Virus and Beet Black scorch Virus

The Application of Renilla luciferase in the Research on Tra...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Wang Guolu Yu Chengming WangDeya Yuan Xuefeng Department of Plant Protection Shandong Agricultural University

The genome of tobacco bushy top virus(TBTV) has a 4152 nt of positive,single-strand RNA(+ssRNA),which lacks 5' cap and 3' poly(A) *** encodes 4 proteins including 0RF1 and ORF2,which are the functional proteins involved in the replication of ***-ORF2 was expressed as 0RF1/0RF2 fused pattern by translational frameshift mechanism,which functions as RNAdependent RNA polymerase(RdRp).Similarly,beet black scorch virus(BBSV) also has a + ssRNA genome of 3644 nt without 5' cap and 3' poly(A) *** genome of BBSV contains 6 ORFs,in which0RF1 and 0RF2 are the components of ***-0RF2 was expressed as ORF1-extended protein by translational read-through mechanism,which also functions as *** using the antibody of TBTV-ORF1 or BBSV-ORF1,the corresponding ORF1 and ORF1/ORF2 fused protein or ORFl-extented protein can be detected with low ratio of frameshift or read-through at <10%.In order to create a more sensitive system to determine the effect of some cis-elements on frameshift in TBTV or read-through in BBSV,Renilla luciferase(Rluc) was fused to the c-terminal of ORF2 in TBTV or *** Rluc-fused TBTV or BBSV,the expression of ORF2 by translational framshift or read-through can be detected with the antibody of Rluc *** addition,the expression of Rluc-associated protein can be detected more sensitively as bioluminescence by providing the *** detailed conditions on this Rluc-associated system are in optimization.

关键词： ORF The Application of Renilla luciferase in the Research on Translational Recoding in Tobacco Bushy Top Virus and Beet Black scorch Virus

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Establish ATMT Mutant Library of Cecrospora zeaemaydis

Establish ATMT Mutant Library of Cecrospora zeaemaydis

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Lu Yuanyuan Xiao Shuqin He Ruihong Zhao Likun Xue Chunsheng Department of Plant Protection Shenyang Agriculture University

Gray leaf spot of corn is an important disease caused by Cecrospora *** present,research on *** mainly focus on the biological characteristics,resistance heredity,evaluation of varieties resources and pathogenesis factors,but less in pathogenesis *** study aims to establish the mutant library by ATMT to based on research the pathogenesis mechanism of *** the basis of hygromycin and green fluorescent protein gene,designs primers to amplify the marker gene,then connected to the backbone vector pPZP100,final constructed the vector with hygromycin and green fluorescent protein gene as a *** the condition of ATMT and establish ATMT mutant library of *** to get 660 *** factors are as follow;spores of *** cultured on V were collected 5d after cultivation and prepared for spore suspension at 10 /mL,then germination 12 h and cultured together with AGL-1(OD =0.5) for 2d in 25℃.Hygromycin B tolerance of *** was 150mg/*** efficiency of transformation is 25/10～5 per milliliter.28 transformants are confirmed by primers form hygromycin gene,which transferred to PDA select medium total 5 time,1300 bp fragment showed that T-DNA had successfully inserted to genome DNA of *** show green fluorescent via the micro-observation.23 strains were random selected through morphological observation,the result show that mutant strains and wild-type strain have significant different with colonial morphology *** side and back side colour of the wild-type strains are mainly black,the transformants strains of positive side are mainly white and red,back side are green *** analysis showed that most transformants strains infected plant,but the pathogenicity obviously weaken.

关键词： Cecrospora zeaemaydis ATMT Mutant library

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The MADS-box transcription FvMcm1 Regulates Conidia Development and Sexual Across in Fusarium verticillioides

The MADS-box transcription FvMcm1 Regulates Conidia Developm...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Yang Cui Liu Huiquan Wang Chenfang Zhao Zhongtao Zhang Shijie Zhang Yimei Li Zhaohui Yin Tao Xu Jinrong State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwestern A&F University

Fusarium verticillioides is one of the most important fungal pathogens to cause destructive diseases of maize *** produced by the fungus are harmful to human and animal *** Fusarium verticillioides,conidia are the primary inoculum and the main source for disease dissemination in the *** this study,we identified and characterized the Fvmcm1 gene that was disrupted in two mating mutants called FGSC7603(FvA149) and FGSC7600(FvA102) producing abnormity microconidian.27%and 14.55%abnormity microcondian were produced in FvA149 and FvA102 mutants149-20 or 102-44,*** of FvMCM1 resulted in the loss of perithecium production and *** rarely produced macroconidia that were abnormal in conidium ***,during microconidium germination,we noticed that the Fvmcm1 mutant(102-44) often produced microconidia instead of germ tubes at early *** Fvmcm1 mutants were reduced to approximately 30%in the production of microconidia in 5-day-old CMC *** yeast two-hybrid assays,FvMCM1 interact with MAT1-1-1 but not with *** Fvmcm1 mutant was defective in phialide formation and defective in self-inhibition of conidium *** results demonstrate that FvMcm1 plays critical roles in the regulation of sexual across,condia development,fumonisin biosynthesis.

关键词： Fusarium verticillioides Condia development Sexual across:FB1

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Bio-control Effect of Rhizobia on Meloidogyne hapla in Peanut

Bio-control Effect of Rhizobia on Meloidogyne hapla in Peanu...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Hui Hongyan Liu Weiyu Wu Xiaojing Wang Yuanyuan Zhu Xiaofeng Chen Lijie Duan Yuxi Nematology Institute of Northern China College of Plant ProtectionShenyang Agricultural University

Root knot nematode is a kind of important pathogen,causing enormous damage to *** has an extensive host range,covering more than 3 000 host plants which belong to 114 *** plant was infected by nematode,its roots became deformity and formeda string of nodular root *** physiological active of the roots were destroyed,which affect the growth of deformity aerial *** always caused great threat on plant growth and huge yield loss when the diseases occurred *** recent years,some studies showed that rhizobia could compete to infection sites with nematodes,and the secondary metabolites from Rhizobia could inhibit the infection of *** control of nematode can be implemented by the screening of high toxic strains of *** September in 2012 to June in 2014,459 soil samples were collected from various ecological systems in Liaoning,Heilongjiang,Inner Mongolia,Jilin,Hebei,Shanxi,Sichuan,Qinghai *** root nodules were obtained from the soil samples with the trap method *** 637 strains of endophytic bacteria were isolated from peanut root *** bio-control rhizobia strains against nematodes could be screened from *** high-toxic strain rhizobia was finally obtained through the first screening with half nodule sieve and the second screening with shake flask *** results indicated that secondary metabolites of 17 endophytic bacteria strains were above 82%in the nodule fatality rate under treating the second juveniles for 24 *** morphological observations,two endophytic bacteria strains were identified as Rhizobium,which fatality rate was above 89%.And the molecular characterization needs to be researched next step.

关键词： Meloidogyne hapla Rhizobia peanuts Endophytic bacteria Biological control

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