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检索条件"任意字段=中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会"

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Cloning and Function Characterization of a Membranebound Transcription Factor Gene TabZIP74 in Wheat

Cloning and Function Characterization of a Membranebound Tra...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Li Yuanyuan Wang Fengtao Lin Ruiming Feng Jing Chen Wanquan Xu Shichang State Key Laboratory For Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

Basic leucine zipper(bZIP) membrane-bound transcription factors(MTFs) comprise a special group in bZIP family,containing a transmembrane domain(TMD) at the *** has been proved that these type transcription factors are key components of signaling pathways in the endoplasmic reticulum(ER) stress ***,there are no reports about bZIP MTFs in *** this study,we cloned a bZIP *** analysis indicated that TabZIP74 contained a 906-bp open reading frame(ORF) encoding a 302-amino-acid *** transcription factor contained a bZIP DNA-bingding domain at its N-terminal and a transmembrane motif at its *** analysis showed that TabZIP74 is highly similar to the function-known ZmbZIP60 and *** expression vector spCam35S;TabZIP74-GFP and pCam35S:TabZIP74℃-GFP were constructed,which contained the fusion gene of TabZIP74 complete ORF and its cDNA fragment in which membrane-bound motif was deleted with the GFP gene respectively,and were transiently expressed in the tobacco epidermal cells mediated by Agrobacterium tumefaciens strain *** florescence observation results showed that the TabZIP74 localized on the cell membrane,but TabZIP74℃ in the *** qRT-PCR results showed that TabZIP14 expression was induced by stripe rust infection,treatments of tunicamycin,MeJA,SA and ET,but was inhibited by salt,drought and ***,TabZIP74 was possibly involved in both defence and stress responses.

关键词： Stripe rust bZIP membrane-bound transcription factor Subcellular localization VIGS Defense response

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Resistance to Barley yellow dwarf virus-GAV and Sitobion avenae for Wheat Resources

Resistance to Barley yellow dwarf virus-GAV and Sitobion ave...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Song Shuang Liu Xiaofeng Zhang Huawei Tao Ye Hao Xingan Wu Yunfeng State Key Laboratory of Crop Stress Biology in Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant ProtectionNorthwest A&F University

Barley yellow dwarf viruses(BYDVs) are recognized as serous pathogens of wheat,which is transmitted by different species of cereal aphids in a circulative,non-propagative *** is critical to find diverse sources of resistance to reduce yield losses caused by virus and its vector *** 2007 to 2013,the identification and evaluation of resistance of 251 wheat resources to BYDV-GAV and its vector Sitobion avenae were carried out by artificial inoculation of viruliferous aphids in the *** results showed five varieties of KOK,KOK IPPCAS,Astron,tpl9 and Amigo-6 from abroad are not only resistant to aphids but also to *** cultivars showed resistance to BYDVGAV,including 00zhong13,Zhong4,Xinong979,Linyou21,878 and *** influence of varieties resistance on the tripartite interactions in a wheat- Sitobion avenae-BYDV-GAV pathosystem had been studied for two years on three wheat varieties,98-10-30(only resistant aphids),Xiaoyan6(not resistant to aphids or virus) and Tam200(13) G(only resistant to virus).The aphid peak number(APN),production of alatae(PA),disease incidence(DIC) and disease index(DID) were measured and analyzed under viruliferous aphid treatment and non-viruliferous aphid treatment for each wheat *** concluded that aphid infestation and virus infection promoted each other under field *** viruliferous aphids caused greater yield reductions than non-viruliferous aphids or virus *** wheat cultivar resistance affected the aphid dispersal,virus transmission,and wheat yield loss obviously.98-10-30 prevented aphid population from increasing,but increased PA,and accelerated virus spread when infested by viruliferous ***200(13) G could prevent the BYDV symptom development,but not virus spread.

关键词： Resistance to Barley yellow dwarf virus-GAV and Sitobion avenae for Wheat Resources

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Functional Analysis of Carbon Catabolite Repressor crel in Antagonistic Trichoderma Strain T23

Functional Analysis of Carbon Catabolite Repressor crel in A...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Li Yaqian Zhou Yucong Xie Qiujin Yu ChuanJin Gao Jinxin Chen Jie School of agriculture and biology State Key Laboratory of Microbial MetabolismShanghai Jiao Tong University

Species belonging to the genus Trichoderma are free-living fungi common in soil and root ecosystems,and have been widely used as antagonistic fungal agents against several phytopathogens as well as plant growth *** CH zinc finger CRE1 protein has been considered a master transcriptional regulator involved in the process of cell growth and glucose assimilations,which promote their competitive and survival in different *** this study,Crel gene and its flanking sequence in *** 23 have been analyzed and cloned by bioinformatics & genetic strategy,and two fragments of PCR product were purified and digested by restriction enzyme and linked to the plasmid pC1300 *** constructed a crel recombinant mutant strain of T23 Acrel by replacing its ORF and part of its 5'-and 3'-regions with a hygromycin B resistance gene under constitutive expression signals using Agrobacterium tumefaciens mediated transformation（ATMT）.The Acrel mutants display severe phenotypic changes such as reduced growth,abnormal hyphal morphology and lag sporulation,comparing with that of wild *** of the strain with a deletion of the crel gene on different medium was *** results showed that the regulation of crel on cell growth and sporulation color depend on the type of *** addition,deletion of crel resulted in an increase in the expression of all the main hydrolytic genes including chitnase and β-1,3- glucanase in chitnase- and glucanase-induced cultures,indicating that CREI acts as a repressor of chitnase and gluconase *** a result,the research revealed that carbon metabolic factor CREI,act as an key global regulator to dominate the growth,sporulation and metabolites of strain *** *** study provides are pretty valuable for the guidance of the control of synthesis and secretion of related metabolites.

关键词： Trichoderma atroviride 23 Carbon catabolite repression crel Sporulation Metabolic regulation Enzyme activity

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The Crystal Structure of MoSubl-DNA Complex with a Novel DNA Binding Mode

The Crystal Structure of MoSubl-DNA Complex with a Novel DNA...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Huang Jinguang Zhao Yanxiang Liu Huaian Huang Dan Zhao Wensheng Neil Justin Ian A.Taylor Liu Junfeng Peng Youliang State key Laboratory of Agrobiotechnology China Agricultrual University MOA Key Laboratory of Plant Pathology China Agricultrual University College of Agronomy and Plant Protection Qingdao Agricultural University

Sub1/PC4,the well-known general transcription cofactor,has multiple functions in transcription and DNA *** we present the crystal structure at 2.7A resolution of MoSub1,the Sub 1/PC4 orthologue from the rice blast fungus,Magnaporthe oryzae,bound to a(dTG) *** structure of the MoSub1-DNA complex superimposes well on to the DNA free structure with an overall RMSD of 1.0A and several key residues undergo small shifts towards the DNA to facilitate *** to PC4,the network of interactions between MoSub1 and DNA is significantly altered although it binds DNA using the same conserved DNA binding ***,the interaction site of MoSub1 with the ssDNA is smaller than that of PC4,at four nucleotides（nt） per monomer rather than the five bound by each PC4 *** nature of the MoSub1/PC4 DNA-protein interface was also probed by mutation of W89 of PC4,a key residue close to the site of DNA conformational change and equivalent to Y74 in *** conservative mutation of W74 Y does not diminish the DNA binding affinity with the dT or *** is still unknown that which one could has a key role in DNA recognition by MoSub1 contributing to both overall binding site size as well as making specific interactions with in the protein-nucleic acid interface.

关键词： MoSub1 Magnaporthe oryzae Sub1/PC4 ssDNA binding protein

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Distribution and Diversity of Cyst-forming Nematodes in China

Distribution and Diversity of Cyst-forming Nematodes in Chin...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Wang Dong Zhu Xiaofeng Wang Yuanyuan Zhu Feng Chen Lijie Duan Yuxi Nematology Institute of Northern China College of Plant ProtectionShenyang Agricultural University

Cyst-forming nematodes are a group of economically important plant pathogens *** date,eighteen cyst nematode species have been documented to occur in *** of our works is to detect and accurately indentify various cyst nematodes from different regions of *** 2010 to 2011,we detected and identified two genera and eight species of cyst nematodes from 10 provinces by comparative morphological and molecular *** approaches based on D2D3 of the 28 S rDNA and rDNA-ITS were applied to study molecular characterization and phylogenetic *** species included Heterodera avenae,***,***,***,***,Cactodera cacti,*** and *** among which *** and *** were new records in China and *** was first reported in Liaoning Province,Northeastern *** cyst nematode(SCN),Heterodera glycines Ichinohe,is the most destructive pest of soybean(Glycines max) in *** another work is to investigate geographical distribution,population densities and virulence phenotypes of *** in *** the survey for SCN from 2012 to 2013,two soil samples from each of 330 soybean fields were collected in main soybean growing zones of 18 provinces;each sample was thoroughly mixed with 5points from each field;over 600 samples were processed for extractions of *** hundred and eighty of the samples had detectable cyst nematode populations,which ranged from 1 to 152 cysts per100cm3 of *** report and our survey indicate SCN has occurred in Anhui,Beijing,Gansu,Guizhou,Hebei,Heilongjiang,Henan,Jiangsu,Jiangxi,Jilin,Liaoning,Neimenggu,Ningxia,Shaanxi,Shandong,Shanxi,Xinjiang and Zhejiang provinces in *** virulence phenotypes can be described with the race determination test or the HG Type *** China,previous data revealed the occurrence of nine *** races including races 1 through 7,9 and 14,whereas HG Types have not been *** 2013,seven indicator

关键词： Cyst nematodes Heterodera glycines Distribution Virulence phenotypes HG Types

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Complete Genome Analysis of a PVYNTN-NW（SRY-I）Recombinant Isolate from Yulin in China

Complete Genome Analysis of a PVYNTN-NW（SRY-I）Recombinant ...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Gao Fangluan Chang Fei Shi Fengyang Shen Jianguo Xie Lianhui Zhan Jiasui Key Laboratory of Plant Virology of Fujian Province Institute of Plant VirologyFujian Agriculture and Forestry University Inspection & Quarantine Technology Center Fujian Exit-Entry Inspection and Quarantine Bureau

To determine the genomic structure of a potato virus Y（PVY） isolate from Yulin,China and its phylogenetic relationship with reported PVY *** complete genome of the PVY ShX14 was amplified and sequenced from overlapping fragments using 11 pairs of primers designed from the conserved regions of the known PVY *** structure and recombination events of the isolate were evaluated by various bioinformatics *** tree was reconstructed by Maximum Likelihood method（ML） using nucleotide sequences of coding *** addition,phylogeny-trait association analysis was used to evaluate the relationship between PVY isolate and known stains *** results show that the complete sequence of ShX14 has 9,724 nucleotides,excluding the 3- terminal poly（A） *** contains a single open reading frame of 9,186 nucleotides and encodes a polyprotein of3 061 amino *** additional protein,termed ' PIPO',is also translated by +2 nucleotide frame shifting within the P3 *** isolate shares 96%9%nucleotide identity and 96%~ 100%amino acid identity with HN2 and SYR-NB-16(PVY strain,SYR-I genotype),*** recombination breakpoints were identified at nucleotide positions 2318,5674 and 8385 with high *** analysis indicates that ShX14 is clustered with PVY（SYR-I）***-trait association and the multiplex PCR analyses also confirm that ShX14 is likely to be a PVY strain（SYR-I）.The above results indicate that ShX14 is a N ×O recombinant isolate,classified to PVY strain（SYR-I）.

关键词： Potato virus Y Complete genome Recombination Phylogeny Phylogeny-trait association analysis

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First Report of Leaf Rust of Blueberry Caused by Pucciniastrum vaccinii in China

First Report of Leaf Rust of Blueberry Caused by Pucciniastr...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhao R J Fu J F Zhou Y Dai H P Yan X R Plant Protection College Shenyang Agricultural University College of Horticulture Shenyang Agricultural University Shenyang Agricultual University

Blueberry is becoming a high-value,niche market crop in *** late September and early October of 2013,rust-like symptoms were observed on high-bush blueberry（Vaccinium corymbosum L.） at a plantation in Liaoning province,which is the largest blueberry-production area in *** our investigation,rust was observed throughout the plants on the cultivars of ' Northblue',' Brigitta',' Bluecrop' and ' Berkeley' in this *** field observations suggest that the plants of ' Northblue' are highly susceptible to the rust and others from ' Bluecrop' shows some *** on the upper surface of leaves began as a chlorotic flecks,then expanded and developed into reddish brown,necrotic spots with a chlorotic halo *** were hypophyllous,numerous yellow rust pustules surrounded by small water-soaked halos appear,which up to about 300μm in diameter,clustered and sometimes *** were subglobose to spherical,（17～22） μm×（12-16） μm（average 19μm× 14μm,n = 50） with hyaline to yellowish,*** morphology and dimensions of urediniospore were consistent with the description of Pucciniastrum vaccinii（***） teleutosorus were not observed in the *** tests were completed with two 12-month-old ' Bluecrop' healthy plants,and another same plant used as control treatment,plants were daub with freshly collected urediniospores from blueberry rust spot,sprayed with a 0.05%solution of Tween 20 in sterile distilled water（SDW）,the control treatment was sprayed with sterile distilled water（SDW）.Then plants were covered with plastic bags for a moisturizing and held in a growth chamber at19 ~ 211 with a 12-h *** spots showed on the upper surface of leaves and yellowish orange pustules were observed blade back after 12 *** characters were the same as observed previously in the field,and the morphology of urediniospore are as same as we observed in the field,whereas no symptoms were observed on

关键词： First Report of Leaf Rust of Blueberry Caused by Pucciniastrum vaccinii in China

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The WRKY Transcription factor,Ta WRKY41,Plays a Positive Role in Wheat Adult Plant Resistance to Puccinia striiformis ***

The WRKY Transcription factor,Ta WRKY41,Plays a Positive Rol...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Fu Yanping Hao Yingbin Chang Haitao Wei Jinping Duan Xiaoyuan Wang Xiaojie Kang Zhensheng State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences Northwest A&F University State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University

Stripe rust,which is caused by Puccinia striiformis ***,is a devastating disease that affects wheat crops *** plant resistance(APR) has recently received much attention due to its non-race-specific and durable *** studies on wheat APR to Pst have involved genetic analyses,but these studies were limited in terms of the examination of transcriptional regulation,particularly for WRKY transcription factors(TFs).Here,we characterize a novel wheat WRKY gene,*** localization demonstrated that TaWRKY41 is a WRKY TF associated with nuclear localization,and TaWRKY41 protein possesses a separate region for mediating transcriptional activation at the C-terminus(381- 430) by yeast one-hybrid *** TaWRKY41 transcript levels were sharply elevated at the early infection stage in adult *** resistance and salicylic acid(SA)-regulated Pathogenesis-Related 1(PR1) gene expression was weakened in TaWRKY41-knockdown adult plants via improved virus-induced gene silencing(VIGS).The silencing of wheat phenylalanine ammonia-lyase(TaPAL),a SA biosynthesis-related gene,resulted in the reduced expression of TaWRKY41 in adult ***,the expression of TaWRKY41 was highly induced after treating adult plants with exogenous *** together,these results showed that TaWRKY41 likely contributes to wheat APR to Pst via the SA pathway.

关键词： WRKY APR wheat Puccinia striiformis f.sp.tritici VIGS.

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Characterization the Regions of the Conserved SNARE YKT6 Gene to Develop a Potential Molecular Target for the Detection of Phytophthora Species

Characterization the Regions of the Conserved SNARE YKT6 Gen...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhao Wei Wang Tao Qi Rende Institute of Plant Protection and Agro-Products Safety Anhui Academy of Agricultural Sciences Scientific Observing and Experimental Station of Crop Pests in Hefei Ministry of Agriculture

The genus Phytophthora consists of many species that are arguably the most destructive plant pathogens causing widespread damage to ornamental,agronomic,and forest ecosystems throughout the world.A variety of molecular techniques have been used for identification of Phytophthora *** most accurate molecular method for identification of isolates to a genus-or species-level is accomplished by development of molecular markers and sequence analysis of specific *** introns of the SNARE-related gene YKT6 were sufficiendy polymorphic for the development of molecular markers for almost all Phytophthora species,with more conserved flanking coding regions appropriate for the design of Phytophthora genus-specific *** Phytophthora genus-specific primer set P-YKT6-F/P-YKT6-R was able to amplify a single band about 600 bp in tested 24 different Phytophthora species,no amplified product from any of the Pythium *** other true fungi *** with the *** specific primer set Ps-YKT6-F/Ps-YKT6-R or *** primer set Pc-YKT6-F/Pc-YKT6-R amplified a specific 399 bp or 282 bp band only from isolates of *** or *** *** *** or ***,the detection sensitivity was achieved to 100 pg and 10 fg in standard PCR and nested PCR,*** primers also proved to be efficient in detecting pathogens from diseased plant tissues,residues and *** addition,real-time quantitative PCR assays coupled with the species-specific primers were developed to detect and quantify the *** results demonstrated that the YKT6-based molecular marker and methods have great potential as a tool for identification and detection of Phytophthora *** a range of applications from pathogen surveys to statutory testing.

关键词： Phytophthora spp. Molecular marker YKT6 PCR

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Exploration of New Genes for Resistance to Magnaporthe oryzae in Oryza glaberrima

Exploration of New Genes for Resistance to Magnaporthe oryza...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Dong Liying Xu Peng Liu Shufang Li Xundong Zhou Jiawu Li Jing Deng Wei Zhang Yu Hu Fengyi Tao Dayun Yang Qinzhong Institute of Agricultural Environment & Resources Yunnan Academy of Agricultural Sciences Institute of Food Crops Yunnan Academy of Agricultural Sciences

Rice blast,caused by filamentous ascomycete Magnaporthe oryzae,is one of the most destructive diseases of rice *** resistance is considered to be the most economic,effective and environmentally responsible method for its control However,resistance genes(R genes) typically lose their effectiveness after a short period in commercial production,due to the emergence of compatible races of *** virulent to R ***,it is necessary to mine new blast R genes with broadspectrum resistance from diverse genetic resources or pyramid R genes with different resistance spectra into a single elite cultivar in order to achieve durable resistance to this *** of new resistance genes to improve resistance to blast from diverse genetic resources is the inevitable choice for *** genus Oryza consists of twenty-one wild and two cultivated ***,the Asian cultivated rice is grown all over the world;The African cultivated rice,*** is only grown in West Africa,and being replaced everywhere by the Asian *** 80 known blast R genes conferring resistance to different races of ***,most of them are identified from Asian cultivated rice(***),with the exception of Pi 9,Pi 33 and Pi 40 genes identified from ***,*** and ***,*** African cultivated rice,*** has been cultivated for more than 3500 years in West *** their yields are lower than the Asian cultivated rice,the previous studies indicated that *** showed highly resistant to rice blast disease in blast nursery of *** order to explore new blast R genes in ***,twenty-four advanced backcrossed populations(BCF) derived from the crosses between 24 accessions of *** and Dianjingyou 1,an *** *** cultivar used as recurrent parent,have been *** 1137 introgression lines(ILs) from the 24 advanced backcrossed populations were inoculated with two high

关键词： Magnaporthe oryzae Resistance gene Exploration Oryza glaberrima

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