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检索条件"任意字段=中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会"

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Molecular Variability and Genome Study of Apple Latent Viruses

Molecular Variability and Genome Study of Apple Latent Virus...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Liu Ping Zhao Lei Hao Xingan Wu Yunfeng State Key Laboratory of Crop Stress Biology in Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant ProtectionNorthwest A&F University

Apple chlorotic leaf spot virus（ACLSV）,apple stem pitting virus（ASPV） and apple stem grooving virus（ASGV） are important viruses infecting apple trees *** occurrence of the three latent viruses in Shaanxi was identified and their molecular variabilities were *** results showed a wide occurrence of three latent viruses in *** relationship was found between the occurrence rate and the apple-producing *** 58.5%samples was infected by single virus of ASGV,followed by 58.5%of ACLSV and 44.0%of *** infection of ACLSV,ASPV with ASGV was 17.8%,which is the most common *** 31 CP gene of ACLSV isolates were cloned（GenBank ID:KJ544862-KJ544880,KF134387-KF134398）.Sequence analysis revealed the pairwise identities ranging from 83%9%and 86%~ 100%at the nucleotide and amino acid level,***31 ACLSV isolates were separated into the P205 and B6 of three groups,*** acid sequences of CP revealed that there was a combination of six amino acids at positions 40,59,75,86,130 and 184 in isolates from each group that could be used to distinguish among the three *** recombination events were identified and three ACLSV isolates collected in this study participated in these two *** isolate of ASPV-YL was *** genome was found to contain five open reading frames（ORFs）.ASPV-YL had a highest identity（79.2%） with an apple isolate from Germany（D21829）,and a lowest identity（71.9%） with a pear isolate from China（EU095327）,which implied that ASPV isolates variables are more related to its hosts than geographic origin.A complete genomic sequence（6495 nt） of a Chinese ASGV isolate from apple（ASGV-CHN） and complete coat protein gene sequences of 14 other ASGV isolates from Shaanxi,China were *** 15 CP gene isolates were separated into two groups regardless of the geographic origin.

关键词： ACLSV ASPV ASGV Molecular varuability Virus genome

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Identification and Breeding of Soybean Cultivars Resistant to Soybean Cyst Nematode in Northeast China

Identification and Breeding of Soybean Cultivars Resistant t...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Chen Jingsheng Li Xiaobai Li Zeyu Tian Zhongyan Duan Yuxi Chen Lijie Nematology Institute of Northern China College of Plant ProtectionShenyang Agricultural University Daqing Branch Heilongjiang Academy of Agricultural Science

Soybean cyst nematode（SCN;Heterodera glycines Ichinohe） is the most major pathogen on soybean production in Heilongjiang *** crop rotation and using of SCN-resistant soybean cultivars were effective in long-term SCN management,and farmers select wisely to sow natural plant resistance soybean in the continuous soybean fields in *** soybean breeding programs continue to find and incorporate new sources of SCN resistance to high-yielding *** 3000 accessions of soybean varieties were evaluated for their resistance to the race 3 of soybean cyst nematode in the *** field identification was based on the egg *** egg population was more than fifty per one hundred grams of dry *** one month,eggs on the soybean varieties were counted,and the number was compared with that from the susceptible ' Hefeng 50' and ' Lee 67'.The level of SCN resistance was estimated based on the Egg Index:EI = number of eggs on a variety × 100/number of eggs on the susceptible *** resistance rating:R = resistant at EI 10%or less;MR = moderate resistant at EI11%0%;MS = moderate susceptible（low resistance） at EI 31%0%;and S = susceptible at EI more than 60%.The results showed that,210 soybean varieties and germplasms were resistant,accounting 7%of the total evaluated *** resistant varieties were qingnong09-1594,qingnong 51062,qingnong1600,qingnong31881 and so *** selected varieties were mostly the long leaf and semi-determinate type in order to adapt to the local ecological *** resistant varieties to SCN were derived from the resistance source ' PI548402（Peking）',' PI437654' and other Chinese black ***,their characteristics were researched in the process of additional field breeding of different *** is a remarkable influence in developing resistant resources for main soybean production area of northeast and the whole *** SCN-resistant materials will provide protection for SCN sustaina

关键词： Soybean(Glycine max) Soybean Cyst Nematode(Heterodera glycines) Germplasm resources Resistance evaluation

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Current Research Progress of Wheat Blue Dwarf Phytoplasma

Current Research Progress of Wheat Blue Dwarf Phytoplasma

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Chen Wang Li Yan Wang Nan Wang Qiang Hao Xing'an Wu Yunfeng State Key Laboratory of Crop Stress Biology in Arid Areas and Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant ProtectionNorthwest A&F University

Wheat blue dwarf(WBD) disease,which was first identified using molecular technique by Gu and Wu in 2005,has caused heavy losses in wheat production in northwestern *** disease is caused by WBD phytoplasma,which is transmitted by Psammotettix *** work indicated that WBD phytoplasma belonged to 16 Sr I *** further research showed the polymorphisms of WBD phytoplasma,three subgroups 16 Sr IB,16 Sr IC and 16 Sr IS were found in WBD ***,some research involved in WBD plasmids,genome and the interaction with periwinkle(Catharanthus roseus) had been ***,three plasmids of wheat blue dwarf(WBD) phytoplasma,named pWBDl(3449 bp),pWBD2(3601 bp) and pWBD3(3844 bp),were cloned and confirmed by southern *** time PCR analysis revealed that the copy numbers of WBD phytoplasma plasmids had tissue specificity in infected periwinkle and changed in the course of ***,the WBD phytoplasma(16Sr IB) draft genome was *** was comprised of six contigs with a total size of611 462 bp,covering-94%of the ***-hundred-twenty-five protein-coding genes,two operons for rRNA genes and 32 tRNA genes were *** pathway analysis indicated that WBD phytoplasma had strongly reduced metabolic ***,46 transporters were identified,which were involved with dipeptides/oligopeptides,spermidine/putrescine,cobalt and Mn/Zn transport,and so on.A total of 37 secreted proteins were encoded in the WBD phytoplasma chromosome and *** these,three secreted proteins were similar to the reported phytoplasma virulence factors TENGU,SAP11 and ***,cDNA-AFLP technique was used to identify transcriptionally regulated genes in WBD phytoplasma-infected ***-hundred-fifty-eight reliable differentially expressed transcript-derived fragments(TDFs) were obtained,and 125 fragments had putative biological functions,such as metabolism,energy,disease/defense,transcription,and so *** TDFs

关键词： Wheat blue dwarf phytoplasma Genome cDNA-AFLP Transcriptionally regulated genes

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Optimize the System of Agrobacterium tumefaciens Mediated Transformation of Curvularia lunata and Knock out ClFtr1

Optimize the System of Agrobacterium tumefaciens Mediated Tr...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Li Hui Xiao Shuqin Zhang Li Zhang Meili Chen Jie Xue Chunsheng Department of Plant Protection Shenyang Agriculture University School of Agriculture and Biology Shanghai Jiaotong University

Curvularia lunata(Wakker) Boed,a causal agent of corn Curvularia leaf spot,has been one of the major leaf diseases in production regions of *** recent years,many studies mainly focused on breeding resistant varieties,occurrence investigation and identification of pathogenic *** have less understanding on relationship between nutrient element iron and pathogenicity mechanismof ***,in this study ATMT target gene knockout system of *** is optimized,and as a platform to knock out the gene ClFtr1,ecoding Fe transporter *** is the foundation for studying ClFtr1 and relationship with pathogenic,simultaneously explaining pathogenicity of *** from iron metabolic,to enrich machanism of molecular *** are as follows:*** of homology comparison showed the genome of *** only had one copy of the gene Ftr1,named ClFtr1(NCBI accession number was GI598062321).The full-length gene of Ftr1 gene was 1317 bp,contained five exons and four introns,ORF was 1030 ***1 genes encoding protein was analyzed by bioinformatics *** showed ClFtr1 protein was highly similar with Ftr1 of ***,such as physicochemical properties,structure,function,etc,and phylogenetic ***,we inferred that ClFtr1 had similar functions with *** Ftr1,participating the transportation in reduced iron absorption ***1 flanking sequences ClFtr1 3R and ClFtr1 5F and hygromycin marker gene were subcloned,and linked into the backbone vector pPZPlOO *** vector pPZP100HC/Ftr1 was verified by restriction enzyme Kpn I *** Freeze-thaw method to Agrobacterium transformation,and using hygromycin and ClFtr1 sequences internal primers to PCR verification,results showed Agrobacterium recombinant was *** of recombinant Agrobacterium OD,conidia concentration and coculture time were optimized,results showed that with the coculture time extension,t

关键词： Curvularia lunata Ftrl gene ATMT Target gene knockout

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Molecular Mapping of Wheat Leaf Rust Resistance Gene in Henong 6251 at Seedling Stage

Molecular Mapping of Wheat Leaf Rust Resistance Gene in Heno...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Ren Xiaodi Du Dongdong Zhang Heshan Hu Yaya Yao Hongpeng Zhao Sai Yang Wenxiang Liu Daqun College of Plant Protection Agricultural University of Hebei Biological Control Center of Plant Diseases and Plant Pests of Hebei Province National Engineering Rese arch Center for Agriculture in Northern Mountainous Areas

Henong 6251 is a new variety of wheat developed by Agricultural University of *** research showed that it contributed higher disease resistance level to some Puccinia triticina *** and mapping wheat leaf rust resistance gene is significant for application of the resistance in the wheat *** parent Henong 6251,susceptible parent Thatcher and their F,F3 populations were employed to mapping the wheat leaf rust resistance genes in the Henong 6251 based on *** marker-assisted identification results showed that Henong 6251 contained leaf rust resistance gene Lr26 and LrZH84,and probably contain Lr2 c and Lr17 *** 6251,Thatcher,and their F,F populations and F3 families were tested for wheat leaf rust resistance with single strain of Chinese *** pathotype PBGP in *** results showed that the wheat leaf rust resistance in Henong 6251 to PBGP was controlled by one dominant resistance gene,temporarily designated as *** molecular markers and BSA were used to test Henong 6251 and their resistant and susceptible bulks of F,F and F populations for mapping the leaf rust resistance *** indicated that Henong 6251 carried a single dominant resistance gene LrH6 to PBGP,and was preliminary located on chromosome *** resistance gene LrH6 was linked to the three known SSR loci（wmc419,wms582 and barc120） on the 1BL with genetic distance ranging 21.6cM,25.8 cM and 27.9 cM *** 6251 contained more than one leaf rust resistance genes including Lr26 and LrZH84 and the wheat leaf rust resistance to PBGP was controlled by one dominant and new resistance gene.

关键词： Wheat leaf rust Resistance gene SSR marker Genetic analysis Genetic mapping

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哈茨木霉T23发酵液对致病病原菌的抑制及体内酶活性的影响

哈茨木霉T23发酵液对致病病原菌的抑制及体内酶活性的影响

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：刘朝辉袁锐杰曾华兰叶鹏盛何炼韦树谷张骞方蒋秋平李琼英曾静四川省农业科学院经济作物育种栽培研究所农业部西南作物有害生物综合治理重点实验室

哈茨木霉T23对茄子黄萎病、烟草黑胫病等土传病害有一定的防治效果。木霉菌对植物病害的生防作用机制主要包括竞争作用、重寄生作用、抗生作用和诱导植物抗性作用等。木霉的次生代谢产物在其防治过程中,抗生作用是防治病害非常重要的机... 详细信息

哈茨木霉T23对茄子黄萎病、烟草黑胫病等土传病害有一定的防治效果。木霉菌对植物病害的生防作用机制主要包括竞争作用、重寄生作用、抗生作用和诱导植物抗性作用等。木霉的次生代谢产物在其防治过程中,抗生作用是防治病害非常重要的机制之一。通过生物信息学分析,引起黄萎病、烟草黑胫病的Verticillium dahliae、Phytophthoraparasitica在NCBI上有与植物类似的PAL、PPO、POD、SOD基因,表明病原菌在受到外界不利因素影响下可能利用类似PAL、PPO、POD、SOD的保护酶进行一定的自我保护。目前,有关木霉代谢产物对病原菌保护酶影响的研究较少,对Verticillium dahliae、Phytophthora parasitica有关保护酶活性的研究就更少。因此,本研究以生防菌哈茨木霉菌株T23,病原菌大丽轮枝菌V8、黑胫病菌HJ12为供试材料,将T23发酵液加入液体培养3天后的V8、HJ12中,同时设加入PD培养基的处理为对照,并于24、48、72、96、120h取V8、HJ12菌丝体,测定这两种病菌的生长量、产孢量、电导率以及过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)和苯丙氨酸解氨酶(PAL)活性,从木霉代谢产物对病菌生长、细胞壁、保护酶活性的影响情况进行综合研究,进一步探讨哈茨木霉发酵液对致病菌生长的抑制作用和机制。研究结果表明,经T23发酵液处理后两种致病菌与对照相比其菌丝体干重的相对生长量呈下降趋势,电导率呈上升趋势,四种保护酶POD、PPO、SOD、PAL的酶活都呈下降趋势。在120h时,大丽轮枝菌、黑胫病菌与对照相比菌丝体相对生长量分别为87.62%、86.58%,与对照相比相对产孢量分别为85.13%、85.27%,与对照相比相对电导率分别为113.47%、117.53%,大丽轮枝菌保护酶POD、PPO、SOD、PAL活性分别下降88.07%、85.49%、92.37%、95.43%,黑胫病菌保护酶POD、PPO、SOD、PAL活性分别下降90.12%、87.39%、90.25%、96.78%。研究表明哈茨木霉菌株T23发酵液可抑制病原菌的生长及孢子的产生,同时可以破坏病原菌的细胞壁致使菌体受到破坏,降低病原菌体内保护酶活性,使得其生理活动不能正常进行,这可能是哈茨木霉菌株T23发酵液抑制致病疫霉生长的机制之一。

关键词：哈茨木霉菌株T23 发酵液大丽轮枝菌黑胫病菌抑菌机制

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长白山区人参菌核病病原生物学及其药剂初筛

长白山区人参菌核病病原生物学及其药剂初筛

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：车喜庆芦小鹏周如军傅俊范沈阳农业大学植物保护学院

人参（Ginsengpanax ***.）属五加科多年生药用植物,具有极高的药用和经济价值。人参菌核病是由土壤传播的最严重的人参病害之一,该病菌株最早于19 1 2年从人参根腐病根上分离得到,1995年确认为核盘菌属一新种——人参核盘菌（Sclerotin... 详细信息

人参（Ginsengpanax ***.）属五加科多年生药用植物,具有极高的药用和经济价值。人参菌核病是由土壤传播的最严重的人参病害之一,该病菌株最早于19 1 2年从人参根腐病根上分离得到,1995年确认为核盘菌属一新种——人参核盘菌（Sclerotinia schinseng Wang et Chen）。迄今国内外仅有少量该病药剂防治、病原菌生态型和致病性分化等相关研究报道,未见人参菌核病发生危害及其病原生物学系统研究报道。本文报道辽宁和吉林人参主产区发生危害,病原生物学及其室内药剂筛选初步研究结果,为该病流行规律及安全防控研究提供科学依据。通过对分离病原菌回接表明所分离菌株对人参具有致病性并证明分离的菌株为致病菌。在PDA培养基上,菌落成辐射状圆形生长,平铺。20℃、6天长满直径90mm的PDA平板,在20℃连续无光照下,8天开始产生菌核。10天左右长满平板菌核。菌核初为白色,渐加深,最后变成黑色,质地粗糙近球形,无光泽,直径（1.88～9.86）mn×（0.86～5.83）mm。该菌与1995年王崇仁等发现的人参核盘菌（Sclerotinia schinseng Wang et Chen）形态一致,确定为该菌。近年来对辽宁和吉林人参主产区病虫害调查发现,人参菌核病发生普遍,为害严重,病田率近90%,病株率5.7%,已经成为长白山区人参主要病害之一。病原生物学研究表明,菌丝在20℃、pH值4、在以Czapek培养基为基础的碳源为山梨醇,氮源为蛋白胨以和胡萝卜培养基上生长最快。菌核萌发最佳条件为20℃、pH值6～8、碳、氮源为乳糖和甘氨酸及PDA培养,菌核形成的最佳条件为20℃、pH值6、碳、氮源为果糖和蛋白胨及PDA培养。黑暗条件促进菌丝生长,有利菌核形成,但对菌核萌发无影响。在对7种不同杀菌剂室内筛选试验,10%苯醚甲环唑水分散粒剂药效最好,其EC为56.04mg/L,40%嘧霉胺悬浮剂最差1 892.024mg/L,其余药剂'70%甲基托布津可湿性粉剂,80%代森锰锌可湿性粉剂,80%腐霉利可湿性粉剂,50%嘧菌酯水分散粒剂,25%啶菌恶唑乳油分别为1 283.714mg/L,3 610.534mg/L,352.804mg/L,1 182.624mg/L,206.134mg/L。本试验研究表明,适宜人参菌核病生长的温度范围在15～20℃,与报道向日葵核盘菌适宜生长范围在20～28℃,西瓜菌核病菌生长范围在20～25℃,一串红菌核病病菌20～25℃有所不同,而与紫花苜蓿18～23℃相近,是由人参菌核病是一种低温病害而产生。其最适生长pH值=6与之前研究报道邵丹辣椒菌核病pH值=3差别较大,与大多菌核病最适pH值=生长范围相差不大。10%苯醚甲环唑水分散粒剂对人参菌核病室内药效最好,田间试验,发生规律及安全控害技术有待进一步研究。

关键词：人参菌核病药剂筛选生物学特性 Sclerotinia schinseng

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小麦白粉病的遥感监测及田间病原菌孢子的时空动态研究

小麦白粉病的遥感监测及田间病原菌孢子的时空动态研究

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：刘伟姚冬明范洁茹陈莉周益林邹亚飞段霞瑜安徽农业大学植物保护学院中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室

小麦白粉病是一种典型的气传病害,在各小麦种植区均有发生。本研究采用近地高光谱遥感和无人机航空遥感技术研究了对小麦白粉病田间病情监测及小麦产量、千粒重和蛋白质含量损失估计的可行性。此外,研究了田间空气中白粉菌分生孢子浓度... 详细信息

小麦白粉病是一种典型的气传病害,在各小麦种植区均有发生。本研究采用近地高光谱遥感和无人机航空遥感技术研究了对小麦白粉病田间病情监测及小麦产量、千粒重和蛋白质含量损失估计的可行性。此外,研究了田间空气中白粉菌分生孢子浓度的变化动态及其与气象因子的关系,建立了基于空气中白粉菌孢子浓度以及气象因子的田间病情预测模型。主要研究结果如下。（1）近地高光谱遥感技术研究表明,小麦白粉病的病情指数在扬花期,灌浆初期和灌浆后期与红边参数中的dλ与SDr具有极显著的负相关性;在植被指数中,参数DVI、RVI、SAVI和NDVI与不同时期的病情指数呈极显著负相关;选取上述参数建立病情指数预测模型,其中基于dλ、DVI、NDVI、SAVI建立的模型均达到较好拟合效果。建立了基于扬花期、灌浆初期的dλ、SAVI产量因子损失率预测模型和基于灌浆后期的dλ、NDVI产量因子损失率预测模型,其中基于dλ所建模型在不同生育时期普适性更好。（2）采用无人机航空遥感技术,将距地面50m、100m、200m、300m和400m的数字图像的颜色特征值R、G、B与病情指数进行相关性分析,结果表明,R、G和B参数与小麦白粉病病情指数之间存在显著或极显著正相关,400m以下高度适合用无人机数字图像参数来估计田间小麦白粉病的发生情况。在相关性分析的基础上,建立了基于亮度I的病情指数、产量、千粒重、蛋白含量的估计模型,各模型均起到较好拟合效果,说明可以利用空中获取的数字图像来估计白粉病对产量、千粒重和蛋白质含量的影响。（3）采用Burkard定容式孢子捕捉器对田间空气中小麦白粉病菌分生孢子的监测结果表明,空气中病菌分生孢子浓度首先随着病情指数的上升而上升,到灌浆期达到最大值,之后逐渐降低。时间序列分析结果表明,两年度田间空气中白粉菌分生孢子浓度均符合ARIMA（1,1,0）模型,而且分生孢子浓度与温度具有显著相关性,由此建立了基于温度的白粉菌分生孢子浓度预测模型,模型均达到显著水平。此外,田间白粉病的发生程度与空气中病菌分生孢子浓度和关键气象因子具有显著相关性,并在此基础上分别建立了基于空气中分生孢子浓度,以及分生孢子浓度和气象因子的田间白粉病情估计模型,其中基于分生孢子浓度的预测模型普适性要优于基于分生孢子浓度和气象因子的预测模型,可以用于田间小麦白粉病的发生预测。

关键词：小麦白粉病高光谱遥感航空遥感数学建模

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不同药剂对根肿菌休眠孢子毒力效果测定及防病应用研究

不同药剂对根肿菌休眠孢子毒力效果测定及防病应用研究

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者：王鹏远蔡丽侯明生湖北省作物病害检测和安全控制重点实验室/华中农业大学

十字花科蔬菜根肿病是一种重要的土传病害,在世界范围内广泛分布。病菌侵染植株在根部形成肿瘤,严重影响植株生长发育,后期可导致全株枯死。目前根肿病在湖北省一些蔬菜种植区均有发生,发病面积多达15万亩,严重影响了十字花科蔬菜产业... 详细信息

十字花科蔬菜根肿病是一种重要的土传病害,在世界范围内广泛分布。病菌侵染植株在根部形成肿瘤,严重影响植株生长发育,后期可导致全株枯死。目前根肿病在湖北省一些蔬菜种植区均有发生,发病面积多达15万亩,严重影响了十字花科蔬菜产业的可持续发展。根肿菌的休眠孢子抗逆能力很强,能长期存活于土壤中,因此生产上对根肿病采取化学药剂防治为主。为了快速筛选一批有效的化学防治药剂,我们预先在室内进行毒力测定,以期获得一批防效较好的可用于大田的防治药剂。本研究结果如下:(1)通过供试药剂对根肿菌休眠孢子致死效果测定,得出了不同时间下,根肿菌休眠孢子在不同药剂处理下的存活率,并明确了存活率存在显著差异的时间阈值,以此作为依据,研究了供试药剂对根肿菌休眠孢子的萌发影响,并通过毒力测定证明了500g/L氟啶胺悬浮剂有着较好的抑菌效果。(2)通过盆栽试验筛选到防治根肿病的高效药剂。试验拌药法和灌药法施药,通过比较10种供试药剂对白菜根肿病的室内盆栽防治效果,从而筛选出高效药剂。结果表明,50%/L氟啶胺悬浮剂处理的防效在80%以上,明显优于其他药剂处理,并将盆栽防治结果与药剂对休眠孢子致死测定、萌发率测定结果进行比较分析,得出药剂防治效果相对一致,组内差异性不显著。(3)对筛选到的4种防效较好的药剂测定其持效期,它们分别为500g/L氟啶胺悬浮剂、72%霜脲·锰锌可湿性粉剂、75%代森锰锌可湿性粉剂、多福可湿性粉剂。72%霜脲·锰锌可湿性粉剂、75%代森锰锌可湿性粉剂20～40天的防效有明显下降趋势,到60天时防效低于50%,两种药剂的持效期仅持续20～40天;多福可湿性粉剂在第60天的防效还在50%以上,500g/L氟啶胺悬浮剂持效期60天的防效仍在80%以上,可满足白菜生长周期的要求。(4)通过室内药剂防治试验,将能有效防治根肿菌休眠孢子的4种药剂进行大田防治试验。试验结果表明,500g/L氟啶胺悬浮剂对大白菜根肿菌的防效与盆栽试验防效均达到80%以上,而72%霜脲·锰锌可湿性粉剂、75%代森锰锌水分散粒剂的防效均低于50%。大田药剂防治试验中,四种药剂在平均株鲜重上表现差异性不显著,从平均亩产量上看,500g/L氟啶胺悬浮剂表现较好,其次是多·福可湿性粉剂。灌根药剂处理后的发病率与平均亩产量呈现负相关性,根肿菌的发病率的高低显著影响白菜的平均亩产量。本研究通过对十字花科蔬菜根肿病进行室内及大田药剂筛选试验,比较了不同药剂对根肿病的防治效果,结果对指导大田根肿病防治具有重要应用价值。

关键词：根肿病芸薹根肿菌药剂筛选防效

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Transcriptome Analysis of Two Different Pathotypes with Different Virulence of Puccinia triticinia

Transcriptome Analysis of Two Different Pathotypes with Diff...

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中国植物病理学会第十届全国会员代表大会暨2014年学术年会及第四届中美植物病理学学术研讨会

作者： Zhang Heshan Du Dongdong Zhang Yumei Yan Hongfei Zhang Na Meng Qingfang Yang Wenxiang Liu Daqun College of Plant Protection Agricultural University of Hebei Biological Control Center of Plant Diseases and Plant Pests of Hebei Province National Engineering Rese arch Center for Agriculture in Northern Mountainous Areas

Puccinia triticinia（Pt） is an obligate parasite pathogen which caused wheat leaf rust all over the world,and causing significant yield losses in wheat production when the diesease developed *** Pt races or new races virulence tendecy allways break the resistance of wheat ***-seq will help to understand how the races changed and which gene contribute the virlence,and help to colong the effectors in *** this research,total RNA from wheat leaves infected with 09-12-284-1 and 09-19-727-1,and urediospores of this two Pt races,were sequenced using Illumina HiSeq 2000 paired-end *** sequencing samples were named as Tc284,Tc727,PT284 and PT727 *** on RPKM method,the significantly differentially expressed genes had 63,which have 34 genes up-regulated and 29 genes down-regulated in Tc727-vs-Tc284.64 significantly differentially expressed genes were detected,of which 62 were up-regulated and 2 were down-regulated in *** significantly differentially expressed genes were up to 7706,which contained 6384 up-regulated and 1322 down-regulated genes in PT284-vs-Tc284.7814 significantly differentially expressed genes had been detected,of which 6572 were up-regulated and 1242 were down-regulated in *** Ontology（GO）,KEGG were used for functional analysis,twele genes selected from the differentially expressed genes in Tc727-vs-Tc284 and PT727-vs-PT284,were suggested be associated with the wheat leaf rust development or *** annotation information included tyrosinase,Hsp20,thaumatin,subtilisin-like serine proteases,glycosyl hydrolase family 18,cytochromeP450,Pheromone A receptor,pectinesterase,major facilitator superfamily,PAZ domain/Piwi domain and major intrinsic *** of 12 had signal peptide,and 4 of them had transmembrane domains analysised by using TMHMM2.0 software,SignaTP 4.1 and Target PL 1.

关键词： Puccinia triticinia Transcriptome sequencing Differentially expressed genes Pathogenicity

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