OBJECTIVES:The main objective of this study was to characterize the molecular characterization of ciprofloxacin-resistant salmonella and the relationship between virulence and resistance of these strains isolated form...
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OBJECTIVES:The main objective of this study was to characterize the molecular characterization of ciprofloxacin-resistant salmonella and the relationship between virulence and resistance of these strains isolated form sick ***:Thirteen non-duplicate ciprofloxac in-resistant salmonella were examined by PCR for plasmid-mediated ESBL and quinolone resistance（PMQR） *** QRDR of topoisomerase genes was also *** detection of efflux pump activities to antibiotic resistance was determined by the addition of carbonyl cyanidem-chlorophenylhydrazone（CCCP） and the organic solvent tolerance *** invasion ability was detected through the adhension and invasion assay to Hela cells,when compared the ciprofloxacin-resistant strains with the susceptible *** this sturdy also detected the expression of the virulence genes using the real-time quantitative PCR（RT-PCR）,the purpose is to further determined the deference of the invasion ability between the ciprofloxacin-resistant strains and the susceptible ***:The qnr,aac（6' ）-Ib-crand oqxA genes were detected in 13 of the isolates and were frequently co-prevalent.7 isolates demonstrated reducing susceptible or low resistant for ciprofloxacin with MIC from 0.125 to 4μg/*** isolates had either single mutation in gyrA or no mutation in any target genes as well the active *** isolates except one presented double mutations in both gyrA and parCand the active efflux conferring high resistance-level to ciprofloxacin（MIC,32-128μg/ml）.The bla type ESBLs were detected in 4 isolates with PMQR presenting high level of resistance to *** the invasion ability of multiple-drug resistant strains was higher than the susceptible strains,but no obvious difference in the adhension *** the expression of the virulence genes -avrA、invA and hilA - and the invasion assay showed the same *** comparision with the susceptible strains,ciprofloxacin-resistant strains s
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