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检索条件"任意字段=中国海洋湖沼学会藻类学分会第十九次学术讨论会"

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Different sensitivity of Ge-Xian-Mi(Nostoc) to ammonium toxicity under two limiting light intensities

Different sensitivity of Ge-Xian-Mi(Nostoc) to ammonium toxi...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Guo-Zheng Dai & Bao-Sheng Qiu~* College of Life Sciences,Central China Normal University,Wuhan 430079,Hubei,P.R.China Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Central China Normal University,Wuhan 430079,Hubei,P.R.China

The sensitivity of Ge-Xian-Mi（Nostoc） to ammonium toxicity under two limiting light intensities(40 and 100μmol photons·m·s)was compared at pH 8.3±0.2 and 25℃.After 96 h exposure to 2 mmol·L NHC... 详细信息

关键词： Different sensitivity of Ge-Xian-Mi Nostoc to ammonium toxicity under two limiting light intensities

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Microwave-assisted staining and in vivo quantification of neutral lipids in Chlorophycean Microalgae

Microwave-assisted staining and in vivo quantification of ne...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Milton Sommerfeld Department of Applied Biological Sciences Arizona State University7001E.Williams Field RoadMesaAZ 85212USA

＜正＞Due to the complexities of lipid analysis by conventional solvent extraction protocols, in vivo determination of neutral lipids with Nile red fluorescence probe has been considered as a useful high-throughput screening method and is now widely ***,the traditional staining protocols was failed to stain some green algal cells in our *** present study employed the microwave-assisted staining technology,for the first time,in fluorescence staining of algal *** on a two-step novel staining protocol for the green algae,this study focused on the optimization of the microwave time for the two staining steps,and different calibration methods for quantitative analysis of neutral lipids in algal cells were further *** the optimized procedure,5μl of green algal cells were mixed with 50μl of DMSO,and then pretreated with microwave for 50 s at a power of 1200 *** the addition of 935μl of deionized water and 10 ul of Nile red,the mixture were stained for 1 min under the assistance of microwave irradiation（1200 W）.Finally,fluorescence emission intensities at 580 nm and excited at 490 nm were recorded for the stained cells,prior to cooling on ice water for 5 *** also indicated that quantification against the intracellular lipids contents could give a accurate results for the tested green ***,the factors that affecting the in vivo quantification of neutral lipids was also investigated and discussed in this study. Results from the present study will provide an important method in the future researches on algae-based biodiesel.

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Photosynthetic recoveries of Nostoc flagelliforme(Cyanophyceae) with different dry storage time during rehydration

Photosynthetic recoveries of Nostoc flagelliforme(Cyanophyce...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Ying-Hui Liu,Lan Yu,Xiang Gao & Bao-Sheng Qiu~* College of Life Sciences,Central China Normal University,Wuhan 430079,Hubei,P.R.China Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Central China Normal University,Wuhan 430079,Hubei,P.R.China

The photosynthetic recovery of Nostoc flagelliforme(Berkeley & Curtis) Bornet & Flahault upon rehydration was investigated with 2-year and 8-year stored dry samples. It was found that 2-year sample absorbed wa... 详细信息

关键词： PSI Cyanophyceae Photosynthetic recoveries of Nostoc flagelliforme with different dry storage time during rehydration

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海带配子体Ihcf5基因的克隆及在雌、雄配子体中的差异表达分析

海带配子体Ihcf5基因的克隆及在雌、雄配子体中的差异表达分析

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者：邹丹燕陆广琴毕燕会周志刚上海海洋大学农业部水产种质资源与利用重点开放实验室上海高校水产养殖学E-研究院

根据海带(Laminaria japonica)雄配子体抑制消减cDNA文库中E04克隆序列及糖海带(***)lhcf5基因的cDNA序列(GenBank登录号:AF226861)设计引物并进行反转录PCR扩增,获得海带雄配子体lhcf5基因的全长cDNA序列(GenBank登录号:EU751620),它长1078 bp,其中5’-非翻译区(UTR)长22 bp,开放阅读框(ORF)长657 bp,3’-UTR长399 bp并具有典型的poly(A)尾巴,且雌、雄配子体lhcf5基因的cDNA序列完全一致。海带配子体lhcf5基因的ORF编码218个氨基酸,与糖海带lhcf5所编码氨基酸序列的相似性高达98%;编码蛋白氨基端的前40个氨基酸组成了跨质体内质网和叶绿体膜信号肽;跨膜酶切后变为含178个氨基酸的成熟蛋白,它的分子量为19.3 kD,等电点为4.86。预测的成熟蛋白LHCF5高级结构存在4个保守性的β-折叠和3个跨类囊体膜的α-螺旋区。利用基因组DNA为模板,锚定PCR扩增产物的序列结果表明海带雌、雄配子体lhcf5基因都存在着一个1134bp的内含子,位于ORF的第62 bp后。实时荧光定量PCR(Q-RT-PCR)结果显示,lhcf5基因在黑暗中接近不转录;雌配子体在40μmol m·s时转录量最高,而雄配子体的最高转录量出现在80μmol m·s的光照强度处;高于最适宜的光照强度,lhcf5基因的转录量会随光照强度增加而降低。相对于同强度的白光来说,短时间(3 d)和长时间(7 d)的红光照射,都会促进这个基因的相对转录水平;蓝光对雌配子体lhcf5基因转录的促进作用不明显,而它对雄配子体lhcf5基因的转录有一定的促进作用。将海带配子体从营养生长转至有利于配子发生的条件下,雌、雄配子体lhcf5基因在第1天的转录量都明显增加;雌配子体在第4天以及第14天显著增加,第6及第10天接近不转录;雄配子体在第8天及第14天增加,第10天也近似不转录;发育阶段其它时间内的转录量都低于营养生长期的。结果表明海带配子体lhcf5的转录受光诱导,其中红光的促进作用最明显;其转录可能因有利于配子体细胞叶绿体结构的完善及物质与能量的积累,而利于细胞分裂与营养生长。

关键词：海带雌配子体雄配子体 lhcf5基因光转录量

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Study on the storage stability of Astaxanthin Esters in Haematococcus pluvialis

Study on the storage stability of Astaxanthin Esters in Haem...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Fengping Miao~(1,2) Yahong Geng~1 Dayan Lu~1 Jincheng Zuo~3 Yeguang Li~1 1 Key Laboratory of Aquatic Botany and Watershed Ecology,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,Hubei 430074,P R China 2 Laboratory of Coastal Zone Bioresources,Yantai Institute of Costal Zone Research for Sustainable Development,Chinese Academy of Sciences,Yantai,Shandong 264003,P R China 3 College of life science,Ludong University,Yantai,Shandong 264025,P R China

The effects of temperature,oxygen,antioxidant and corn germ oil on the stability of astaxanthin, as well as changes of each component of astaxanthin esters in Haematococcus pluvialis under different storage conditions... 详细信息

关键词： Haematococcus pluvialis astaxanthin ester storage stability Storage Life HPLC

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Polysaccharide release by Aphanothece halophytica inhibits cyanobacteria/clay flocculation

Polysaccharide release by Aphanothece halophytica inhibits c...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： CHEN Li~1,MEN Xiao~1,MA Meirong~1,LI Pengfu~(1,*),JIAO Qingcai~1,LU Shan~1, KONG Fanxiang~2 and WU Shengcai~3 1 School of Life Sciences,Nanjing University,Nanjing 210093,P.R.China 2 State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,73 East Beijing Road, Nanjing 210008,P.R.China 3 Department of Biology,Yancheng Normal University,Yancheng 224002,Jiangsu,P. R.China

Many microalgae release polysaccharides,but the effects of the polysaccharides on mutual flocculation of microalgae and clay in aquatic environments have not been well *** halophytica is a bloom-forming cyanobacterium... 详细信息

关键词： RPS Polysaccharide release by Aphanothece halophytica inhibits cyanobacteria/clay flocculation

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Genetic Analysis of Laminaria japonica Sporophytes Produced by Crossing of Gametophyte Clones of Different Lines

Genetic Analysis of Laminaria japonica Sporophytes Produced ...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Li Dapeng,Wu Chaoyuan,Peng Guang (Institute of Oceanology,Chinese Academy of Sciences 266071,

one-celled female and male gametophytes of Laminaria japonica were isolated,cultured and gametophyte clones were *** the basis of knowledge of growth and development of gametophyte clones,Experiment on 14 different crossing combinations was *** economic characteristics,frond length,fresh weight and iodine content,of F sporophyte of different combinations were *** result showed,there were significant differences in morphology among different combination,and sporophytes in the same combination were almost morphologically *** facts that there were significant differences among combinations and similarities in the same combination in frond length and fresh weight should be mainly attributable to genetic factors.F1 sporophytes of No.1 and No.2 showed a higher average fresh weight and longer frond length,therefore,No.1 Wh(♀×♂) and No.2 (Wh860♀×Lid♂) were selected as good combinations.

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Ecophysilogical characteristics of aerial microalga Trentepohlia aurea

Ecophysilogical characteristics of aerial microalga Trentepo...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： Liu Jianguo~(1,4) Liu Qian~(1,2) Zhang Yutao~(1,3) Zhang Yong~4 Liu Chenlin~5 Lin Wei~1 Huang Xiaohang~5 1 Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,PR China 2 Faculty of Life Sciences and Engineering,Ningbo Uiversity,Ningbo 315211,PR China 3 Department of Plant Science,Shandong Agriculture University,Taian 271018,PR China 4 Yunan Alphy Biotech Corporation Limited,Chuxiong 76500,PR China 5 The first institute of Oceanography,State Oceanic Administration,Qingdao 266061,China

The ecological distribution and physiological property of Trentepohlia aurea,an aerial microalga in west plateau klint of Sichuan Basin,China have been *** data showed that *** wide distributed in the range of Heavy Snow Mountains and QiongLai Mountains at 1500-5000 meters altitude,which at least cover 30,000 square Km in Wenchuan,Xiaojin,Danba,Daofu,Kangding and

关键词： Ecophysilogical characteristics of aerial microalga Trentepohlia aurea

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Microbial degradation and preliminary chemical characterization of Microcystis exopolysaccharides from a cyanobacterial water bloom of Lake Taihu

Microbial degradation and preliminary chemical characterizat...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者： LI Pengfu~(1,*),CAI Yuanfeng~1,SHI Limei~1,GENG Lanfang~(1,3),XING Peng~2,YU Yang~2,KONG Fanxiang~(2,*),WANG Yujiong~3 1 School of Life Sciences,Nanjing University,Nanjing 210093,P.R.China 2 State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,73 East Beijing Road, Nanjing 210008,P.R.China 3 College of Life Sciences,Ningxia University,Yinchuan 750021,P.R.China

The bloom biomass composed mainly of Microcystis *** harvested from Lake Taihu,***(FEPS,exopolysaccharides from filed-grown Microcystis spp.) were extracted from the bloom mass with 3%*** contained 5.4%protein and 6.9... 详细信息

关键词： Microbial degradation and preliminary chemical characterization of Microcystis exopolysaccharides from a cyanobacterial water bloom of Lake Taihu

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The response and funtion of D1 protein to solar ultraviolet radiation in Arthrospira platensis

The response and funtion of D1 protein to solar ultraviolet ...

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庆祝中国藻类学会成立30周年暨第十五次学术讨论会

作者：吴海燕吴红艳汕头大学海洋生物研究所

Previous studies have shown that solar ultraviolet radiation could lead to the photoinhibition of Arthrospira platensis and the turnover of D1 protein is involved in the repair of the UV-B induced PSⅡ*** this study,cultures of *** 439 were exposed to full spectrum of solar radiation(PAB,280-700nm),solar radiation depleted of UV-B(PA,320-700nm) and solar radiation filtered out of UV-B + UV-A(P,400-700nm) in order to investigate the synergic effects of PAR and UV radiation on the changes of the amount of D1 protein and the role of de novo synthesis of D1 protein during the exposure to solar UV radiation..It was shown that at irradiance level of PAR120 W m,UV-A 27.5 W m and UV-B 0.84 W m,the effective photochemical efficiency(Fv’/Fm’) of *** decreased to 73.7%,40.5%and 35.1%of the initial value under P,PA and PAB treatments *** in the presence of lincomycin,an inhibitor of protein synmesis,Fv’/Fm’ decreased sharply to 7.9%under P treatment,and could not be detected under PA and PAB *** irradiance level of PAR 320 W m,UV-A 73.6 W m,UV-B 2.24 W m led to the inhibition of the photosynthetic activity to a much more extent,Fv’/Fm’ could not be detected regardless of the radiation treatments in the presence of *** oxygen evolution rate was also affected by UV radiation,but to a less extent as compared with the photochemical efficiency both in the absence and presence of *** addition,the relative electron transfer rate(rETR) was significantly decreased under the radiation treatment with UVR, showing an inhibition of 44.6%and 54.8%in PA and PAB treatments compared with that under PAR,while rETR could not be examined when the D1 protein synthesis was *** amount of D1 protein examined by western blot showed that it could be damaged by solar radiation treatments,particularly at high irradiance ***,when the synthesis of D1 protein was inhibited by lincomycin,it showed a much lower content.P t

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