利用厌氧氨氧化菌16S rRNA基因特异引物对红壤稻田土壤DNA进行PCR-DGGE(聚合酶链反应-变性梯度凝胶电泳)结合DNA克隆测序，研究了氮肥供应量对厌氧氨氧化菌群落结构的影响.DGGE图谱及依据其条带位置和亮度数值计算的多样性指数均显示：高氮处理[N3：225 kg (N)hm-2]的厌氧氨氧化菌群落结构多样性在根层或表层土壤中均显著(P＜0.05)高于中、低氮[N2：150 kg (N) hm-2;Nl：75 kg (N) hm-2]和不施肥对照(CK);且高氮处理下表层土壤厌氧氨氧化菌群落多样性指数显著高于根层土壤(P＜0.05).冗余分析(RDA)结果表明表层土壤中厌氧氨氧化菌群落结构组成与不同氮肥水平处理存在显著相关性(P=0.006).同时获得厌氧氨氧化菌DGGE条带DNA序列18条登录GenBank.研究表明红壤稻田厌氧氨氧化菌群落结构对高氮水平具有较强的响应，尤其是在表层土壤中.
Qingcaosha Reservoir is located at Yangzhi Estuary River, and it is the largest tidal reservoirs in the *** Qingcaosha reservoir serve as a strategic water source of Shanghai, and has the strategic importance of safeg...
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Qingcaosha Reservoir is located at Yangzhi Estuary River, and it is the largest tidal reservoirs in the *** Qingcaosha reservoir serve as a strategic water source of Shanghai, and has the strategic importance of safeguard the safety of drinking water and economic development in Shanghai.
Cyanobacterial blooming has become frequent as a result of eutrophication in *** accumulation and breakdown of huge cyanobacterial biomass often causes hypoxia in ***, not much is known about microbial diversity in th...
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Cyanobacterial blooming has become frequent as a result of eutrophication in *** accumulation and breakdown of huge cyanobacterial biomass often causes hypoxia in ***, not much is known about microbial diversity in these areas.
Attached microbial communities are increasingly recognized as important sites for environmental mercury methylation and show higher methylation rates than unattached *** inhibition of methylation by chloroform and inc...
Attached microbial communities are increasingly recognized as important sites for environmental mercury methylation and show higher methylation rates than unattached *** inhibition of methylation by chloroform and increased expression of cooS indicate a significant role for the of the acetyl-CoA pathway in methylation by biofilms of Desulfovibrio desulfuricans.
With the rapid development of social economy,excess influx of nutrients from anthropogenic source, such as wastewater and runoff from fertilized fields into aquatic ecosystems, causes eutrophication and bloom in a lar...
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With the rapid development of social economy,excess influx of nutrients from anthropogenic source, such as wastewater and runoff from fertilized fields into aquatic ecosystems, causes eutrophication and bloom in a large area of red tide in coastal region, even brings to ecosystem deterioration.
In general, the bioleaching environment is very hostile for the survival of microorganism, because there are excess heavy metal ions, such as copper, silver, arsenic and so on, which are harmful to the growth metaboli...
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In general, the bioleaching environment is very hostile for the survival of microorganism, because there are excess heavy metal ions, such as copper, silver, arsenic and so on, which are harmful to the growth metabolism of organisms.
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