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Cleavage-Site of a Novel Ribosome-Inactivating Protein on Eukaryotic Ribosomal RNA

Cleavage-Site of a Novel Ribosome-Inactivating Protein on Eu...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者：何文君徐洪刘望夷 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences .Chinese Academy of Sciences. Shanghai 200031 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences .Chinese Academy of Sciences. Shanghai 200031 Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences .Chinese Academy of Sciences. Shanghai 200031

AIM:To study the mechanism of action of a novel ribosome-inactivating protein(RIP)on the eukaryoticribosomal ***:By in vitro inhibition to protein synthesis and detection of the diagnostic RNAfragment produced to assa... 详细信息

AIM:To study the mechanism of action of a novel ribosome-inactivating protein(RIP)on the eukaryoticribosomal ***:By in vitro inhibition to protein synthesis and detection of the diagnostic RNAfragment produced to assay the activity of *** cleavage-site of this RIP on the 28S ribosomal RNA wasidentified by RNA sequencing and two-dimensional chromatography of ***:In the seeds ofgymnosperm was isolated a novel RIP that was capable of inactivating rat ribosome and of inhibiting protein synthe-

关键词： Cleavage-Site of a Novel Ribosome-Inactivating Protein on Eukaryotic Ribosomal RNA domain

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Localisation of the IL-1 receptor to focal adhesions and ruffles in attached and migrating fibroblasts-Regulation through the C-terminal end of the cytoplasmic domain

Localisation of the IL-1 receptor to focal adhesions and ruf...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者： Valles S. Carlotti F. Qwarnstrom E.E. Functional Genomics Division of Molecular and Genetic Medicine University of Sheffield UK Functional Genomics Division of Molecular and Genetic Medicine University of Sheffield UK Functional Genomics Division of Molecular and Genetic Medicine University of Sheffield UK

Analysis of the mechanisms involved in translocation of the IL-1 receptor to focal adhetions was carried out byconfocal microscopy of cells transfected with a series of receptor constructs and using EGFP(enhanced gree... 详细信息

Analysis of the mechanisms involved in translocation of the IL-1 receptor to focal adhetions was carried out byconfocal microscopy of cells transfected with a series of receptor constructs and using EGFP(enhanced greenfluoresent protein),as the *** cells attached to fibronectin,the receptor fusion protien was located to distinct

关键词： Localisation of the IL-1 receptor to focal adhesions and ruffles in attached and migrating fibroblasts-Regulation through the C-terminal end of the cytoplasmic domain domain

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A New Sterol from the Brown Algae Sargassum Carpophyllum and Ishige Okamurai

A New Sterol from the Brown Algae Sargassum Carpophyllum and...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者： Hai-Feng Tang yang-hua yi xin shengyao qiang zhi xu kun ma and yong pei jiang(researchcenter for marine drugs school of pharmacy second military medical university shanghai 200433)

Bioassay-guided fractionation of the extracts of Sargassum carpophyllum and Ishige okamurai,has led to theisolation and identification of new sterols 1a and 1b as an inseparable mixture. A known hydroperoxysterol 2 wa... 详细信息

Bioassay-guided fractionation of the extracts of Sargassum carpophyllum and Ishige okamurai,has led to theisolation and identification of new sterols 1a and 1b as an inseparable mixture. A known hydroperoxysterol 2 was alsoisolated from the former extract. These compounds induced morphological abnormality of Pyricularia oryzae myce-

关键词： A New Sterol from the Brown Algae Sargassum Carpophyllum and Ishige Okamurai

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Characterization and Molecular Cloning of a Superoxide Dismutase Purified from the Seeds of Camphor Tree

Characterization and Molecular Cloning of a Superoxide Dismu...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者：陈淮扬陈文锋汪宝忠刘望夷 Werner Schrder Peter Frank Norbert Ulbrich Freie Universitat Berlin Institut für Biochemie Fabeckstr.36a.D14195 Berlin Freie Universitat Berlin Institut für Biochemie Fabeckstr.36a.D14195 Berlin Universitat sklinikum Charite Medizintsche Universitsklinik Ⅲ AG Experimentelle Rheumatologie und Biochemie Tucholsky Str.2 10117 Berlin Germany

An iron-superoxide dismutase(SOD)was purified and characterized from the seeds of camphor tree(Cinnamomum camphora).The camphor Fe-SOD activity has been tested by three *** camphor Fe-SODactivity is inhibited neither ... 详细信息

An iron-superoxide dismutase(SOD)was purified and characterized from the seeds of camphor tree(Cinnamomum camphora).The camphor Fe-SOD activity has been tested by three *** camphor Fe-SODactivity is inhibited neither by 1-5 mM of cyanide nor by 1-30 mM of sodium azide,but by 2-5 mM of hydrogen

关键词： SOD Characterization and Molecular Cloning of a Superoxide Dismutase Purified from the Seeds of Camphor Tree

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Basic chromic salt,a wonderful sensitivity-enhancer of silver staining

Basic chromic salt,a wonderful sensitivity-enhancer of silve...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者：周志东李民乾刘望夷 Institute of Biochemistry and cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320Yue-Yang road Shanghai 20031 Shanghai Instrtute of Nulear Research Chinese Academy of Sciences 2019 Jia-Luo Road Shanghai 201800 Shanghai Instrtute of Nulear Research Chinese Academy of Sciences 2019 Jia-Luo Road Shanghai 201800 Institute of Biochemistry and cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320Yue-Yang road Shanghai 20031

Basic chromic salt,a new semsitivity enhancer of silver staining,was found that it could be combined withdifferent sliver staining procedures conveniently,diamine or non-diamine stainings,with obvious sensitivity *** ... 详细信息

Basic chromic salt,a new semsitivity enhancer of silver staining,was found that it could be combined withdifferent sliver staining procedures conveniently,diamine or non-diamine stainings,with obvious sensitivity *** standard enhancing procedure was convenient with only 5-10 minutes of reaction and wash twiceeach for 10 *** maximum sensitivity of double staining with coomassie brilliant blue R-250 could almost

关键词： Basic chromic salt,a wonderful sensitivity-enhancer of silver staining

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Expression,refolding and modelling of the camphor superoxide dismutase

Expression,refolding and modelling of the camphor superoxide...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者：胡荣贵汪宝忠徐洪刘望夷阮康成 Institute of Biochemistry and Cell Bioiogy Shanghai Institutes for Biologrcal Sciences Chinese Academy of Sciences Shanghai 200031 Institute of Biochemistry and Cell Bioiogy Shanghai Institutes for Biologrcal Sciences Chinese Academy of Sciences Shanghai 200031 Institute of Biochemistry and Cell Bioiogy Shanghai Institutes for Biologrcal Sciences Chinese Academy of Sciences Shanghai 200031 Institute of Biochemistry and Cell Bioiogy Shanghai Institutes for Biologrcal Sciences Chinese Academy of Sciences Shanghai 200031 Institute of Biochemistry and Cell Bioiogy Shanghai Institutes for Biologrcal Sciences Chinese Academy of Sciences Shanghai 200031

The cDNA of camphor Fe-SOD was cloned into the expression vector PMFT7-5 and then expressed in *** strain BL21(DE3).A large amount of the target protein was recovered in the inclusion *** incorporation ofmetal cofacto... 详细信息

The cDNA of camphor Fe-SOD was cloned into the expression vector PMFT7-5 and then expressed in *** strain BL21(DE3).A large amount of the target protein was recovered in the inclusion *** incorporation ofmetal cofactors,ferrous or manganese sulfate were added to the 8 M urea solution of inclusion *** recon-structed Fe-or Mn-SOD was purified to homogeneity through columns of DEAE-cellulose 52 and Bio-gel P-100.

关键词： SOD Expression,refolding and modelling of the camphor superoxide dismutase

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Crystal structure of a human rhinovirus type 14(HRV14):human immunodeficiency virus type 1(HIV-1)V3 loop chimeric virus that elicits a neutralizing antibody response against HIV-1

Crystal structure of a human rhinovirus type 14(HRV14):human...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者： Ding J. Smith A.D. Geisler S.C. Arnold G.F. Arnold,E Center for Advanced Biotechnology and Medicine(CABM)and Rutgers University Chemistry Department 679 Hoes Lane Piscataway NJ08854-5638、USA Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai 200031 Center for Advanced Biotechnology and Medicine(CABM)and Rutgers University Chemistry Department 679 Hoes Lanc Piscataway NJ 08854-5638 USA Center for Advanced Biotechnology and Medicine(CABM)and Rutgers University Chemistry Department 679 Hoes Lanc Piscataway NJ 08854-5638 USA Center for Advanced Biotechnology and Medicine(CABM)and Rutgers University Chemistry Department 679 Hoes Lanc Piscataway NJ 08854-5638 USA Center for Advanced Biotechnology and Medicine(CABM)and Rutgers University Chemistry Department 679 Hoes Lanc Piscataway NJ 08854-5638 USA

The structure of a chimeric human rhinovirus type 14(HRV14) that displays on its surface an immunogenic insert from the gp120 V3 loop of the human immunodeficiency virus type 1 (HIV-1) has been solved at 2.7 A *** vir... 详细信息

The structure of a chimeric human rhinovirus type 14(HRV14) that displays on its surface an immunogenic insert from the gp120 V3 loop of the human immunodeficiency virus type 1 (HIV-1) has been solved at 2.7 A *** virus, designated MN-III-2, was isolated from a combinatorial library by immunoselecting with a variety of

关键词： HIV-1)V3 loop chimeric virus that elicits a neutralizing antibody response against HIV-1 HRV14 Crystal structure of a human rhinovirus type 14 human immunodeficiency virus type 1

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Optimization of multiple factors in the discovery and development of highly potent and broad spectrum non-nucleoside HIV-1 reverse transcriptase inhibitors for the treatment of AIDS

Optimization of multiple factors in the discovery and develo...

中国生物化学与分子生物学会第八届会员代表大会暨全国学术会议

作者： E.Arnold K.Das A.D.Clark.Jr. D.Ludovici M.Kukla B.DeCorte K.Andries R.Pauwels M.-P.Bethune P.Lewi S.H.Hughes P.A.J.Janssen Center for Advanced Biotechnology and Medicine and Rutgers University Department of Chemistry Piscataway NJ Center for Advanced Biotechnology and Medicine and Rutgers University Department of Chemistry Piscataway NJ Center for Advanced Biotechnology and Medicine and Rutgers University Department of Chemistry Piscataway NJ Janssen Research Foundation Springhouse PA Janssen Research Foundation Springhouse PA Janssen Research Foundation Springhouse PA Janssen Research Foundation Beerse Belgium Tibotec.Mechelen.Belgium Tibotec.Mechelen.Belgium Center for Molecular Design Janssen Research Foundation Vosselaar Belgium HIV Drug Resistance Program NIH National Cancer Institute-Frederick Frederick MD Center for Molecular Design Janssen Research Foundation Vosselaar Belgium

A multidisciplinary approach has led to the discovery of novel non-nucleoside HIV-1 reverse transcriptaseinhibitors(NNRTIs)that show great promise as drug candidates for the treatment of *** the design

A multidisciplinary approach has led to the discovery of novel non-nucleoside HIV-1 reverse transcriptaseinhibitors(NNRTIs)that show great promise as drug candidates for the treatment of *** the design

关键词： HIV Optimization of multiple factors in the discovery and development of highly potent and broad spectrum non-nucleoside HIV-1 reverse transcriptase inhibitors for the treatment of AIDS

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帕金森病模型大鼠纹状体内褪黑素与多巴胺及其代谢产物的变化

帕金森病模型大鼠纹状体内褪黑素与多巴胺及其代谢产物的变化

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者：孟涛林玲郑志竑福建医科大学生物化学与分子生物学系福建医科大学神经生物学研究中心

目的探讨帕金森病(Parkinson's disease,PD)模型大鼠纹状体内褪黑素与多巴胺及其代谢产物的变化。方法 6-羟基多巴胺定点注射至大鼠右侧黑质致密部和前脑内侧束建立PD大鼠模型,采用脑微透析术分别于建模后3 w、4 w、5 w、6 w 22:00... 详细信息

目的探讨帕金森病(Parkinson's disease,PD)模型大鼠纹状体内褪黑素与多巴胺及其代谢产物的变化。方法 6-羟基多巴胺定点注射至大鼠右侧黑质致密部和前脑内侧束建立PD大鼠模型,采用脑微透析术分别于建模后3 w、4 w、5 w、6 w 22:00收集脑微透析液,结合高效液相色谱(HPLC)动态监测毁损侧纹状体内多巴胺及其代谢产物水平,并结合酶联免疫分析(ELISA)动态检测毁损侧纹状体内褪黑素水平。结果 PD模型组大鼠纹状体内多巴胺(DA)、二羟基苯乙酸(Dopac)及高香草酸(HVA)水平均明显低于溶剂对照组及假手术组,以建模后3 w降低最为显著(分别降低86.28%、80.51%、81.50%)。建模后3 w、4 w PD模型组大鼠纹状体内褪黑素水平与其余两组无显著差异,而5 w、6 w褪黑素水平较其余两组大鼠明显增高(分别增高1.31倍和1.34倍),而溶剂对照组及假手术组两者之间无显著差异。结论 6-羟基多巴胺PD模型大鼠晚期阶段褪黑素可能影响多巴胺及其代谢产物的合成及释放。

关键词：帕金森病多巴胺褪黑素脑微透析

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中国生物化学与分子生物学会2001年活动计划表

生命的化学 2001年第1期21卷 89-89页

序号会议名称主要内容时间规模(人) 地点联系人电话 1 第九次全国生物化学与分子生物学学术会议暨第八届中国生物化学与分子生物学会会员代表大会为我学会四年一次的学术大会，全国生化界学者共同讨论、交流21世纪生化及分子生... 详细信息

序号会议名称主要内容时间规模(人) 地点联系人电话 1 第九次全国生物化学与分子生物学学术会议暨第八届中国生物化学与分子生物学会会员代表大会为我学会四年一次的学术大会，全国生化界学者共同讨论、交流21世纪生化及分子生物学领域的发展方向；同时汇报中国生物化学与分子生物学会七届理事会的工作，进行新一届理事会的换届选举工作 9月12—14 日 400 上海王悦王同喜 010-64889892021-64310851 2 天然活性物质的研究开发及其产业化学术研讨会和产业化等方面问题的研讨各类天然活性物质的研制、开发 9月 100至150 大连金新根唐炳琴 021-64253238021-64253019 3 第二届二十一世纪农业生物技术展望论坛会 1.基础研究与农业生物技术发展2.生物信息与农业生物技术发展3.农业生物技术新进展4.农业生物技术产业化与生态保护 10月 150 待定马仁懿 010-62891836 4 第六届全国脂蛋白与动脉硬化学术讨论会脂蛋白的结构与功能以及动脉硬化的发病机制 7—8月 120至150 烟台姜安丽 0531-2942092 5 生物技术培训班生物学技术的实验方法教学 8月 30 北京马仁懿许雷 010-62891836 6 分子生物学技术培训班分子生物学技术的基本技术和新方法 8月 30 北京魏群北京师范大学生命科学学院

关键词：中国生物化学分子生物学技术学会活动生物技术产业化农业生物天然活性物质脂蛋白培训班北京学术讨论会二十一世纪硬化学术研讨学术会议学术大会选举工作师范大学生物信息

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