Isocitrate dehydrogenase kinase/phosphatase(AceK) is a bifunctional enzyme with both kinase and phosphatase activities that are activated by *** have studied the interactions of Mnand Mg with AceK using isothermal t...
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Isocitrate dehydrogenase kinase/phosphatase(AceK) is a bifunctional enzyme with both kinase and phosphatase activities that are activated by *** have studied the interactions of Mnand Mg with AceK using isothermal titration calorimetry(ITC) combined with molecular docking simulations and show for the first time that Mn also activates the enzyme *** Mn and Mg exert their effects by different *** they have similar binding constants(of 1.11×10~5 and 0.98×10~5 M,respectively) for AceK and induce conformational changes of the enzyme they do not compete for the same binding *** Mn appears to bind to the regulatory domain of AceK,and its effect is transmitted to the active site of the enzyme by the conformational change that it induces.
Alternative splicing (AS) is an important mechanism for controlling gene expression, which regulates multiple biological processes in higher organisms. Chinese indigenous Xiang pig has distinctive biological character...
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Alternative splicing (AS) is an important mechanism for controlling gene expression, which regulates multiple biological processes in higher organisms. Chinese indigenous Xiang pig has distinctive biological characteristics, such as small size,early sexual maturity, lower litter size and not very clear exhibition of estrous behaviors. To further understand how AS responds to estrous cycles in Xiang pig, the genome-wide analysis of AS events was performed by RNA-seq method in Xiang
Microglial DICER was phosphorylated at serine 1456 by c-jun N-terminal kinase(JNK) and downregulated in response to 1-methyl-4-phenylpyridinium(MPP+),a causative agent in *** of JNK phosphorylation of DICER at serine ...
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Microglial DICER was phosphorylated at serine 1456 by c-jun N-terminal kinase(JNK) and downregulated in response to 1-methyl-4-phenylpyridinium(MPP+),a causative agent in *** of JNK phosphorylation of DICER at serine 1456 rescued the MPP+-induced DICER degradation,suppressed microglial inflammatory process,and prevented the loss of tyrosine hydroxylase-expressing neurons in the mouse MPTP ***-mediated microglial DICER degradation potentiates inflammation to induce dopaminergic neuronal ***,preventing microglial DICER degradation could be a novel strategy for controlling neuroinflammation in PD.
In this work,based on the high fluorescence quenching efficiency of polydopamine nanospheres(PDANS) and the specific recognition characteristic of aptamer,we construct a rapid,simple,sensitive and economical fluoresce...
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In this work,based on the high fluorescence quenching efficiency of polydopamine nanospheres(PDANS) and the specific recognition characteristic of aptamer,we construct a rapid,simple,sensitive and economical fluorescent biosensor for "turn-on" detection of ochratoxin A(OTA).In the absence of OTA,the fluorescence of FAM was readily quenched due to the strong binding between aptamer and *** the presence of OTA,the conformation of the OTA-specific aptamer changed from a random-coil to an antiparallel G-quadruplex,which resulted in the dissociation of the FAM from the surface of PDANS and the increasing of fluorescence *** method allowed quantitative detection of OTA with a detection limit of 20 ***,it was applied for the detection of OTA in red wine samples and showed satisfactory results.
Cytosolic RNA/DNA sensing warns host cells both microbe invasion and cellular damage,and to initiate counter measures by igniting cascades through MAVS/STING adaptors,TBK1/IKKεkinases and IRF3 transcription ***,how t...
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Cytosolic RNA/DNA sensing warns host cells both microbe invasion and cellular damage,and to initiate counter measures by igniting cascades through MAVS/STING adaptors,TBK1/IKKεkinases and IRF3 transcription ***,how the magnitude of this recently defined innate immune sensing is controlled remains largely *** functional screens of proteomics and analyses of interactome,we defined a few interesting players in governing of cytosolic nucleic acid sensing,via manipulating of phosphate moieties or changing cellular address of *** findings identify some physiological regulators of innate immune sensing,and provide mechanistic insights as well as new prevention strategies for both pathogen infection and tumorigenesis.
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