检索结果-内蒙古大学图书馆

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Ying Shi Tong Cao WenJing Zhou Yue Cui Yu Sun Z.Peter Wang JIA MA Department of Biochemistry and Molecular Biology School of Laboratory MedicineBengbu Medical College Research Center of Clinical Laboratory Science Bengbu Medical College Center of Scientific Research The Second Affiliated Hospital of Wenzhou Medical University

Nitidine chloride(NC) exhibits tumor suppressive function in a variety of human cancer ***,the molecular mechanism of NC-triggered anti-cancer activity has not yet been completely *** aim of the present study was to investigate the anti-tumor molecular basis of NC in prostate cancer *** present study demonstrated that NC inhibited cell growth and induced apoptosis in prostate cancer *** addition,NC suppressed cell migration and invasion in prostate cancer ***,NC was demonstrated to decrease the expression of the oncoprotein Yes-associated protein(YAP) in prostate cancer *** downregulation of YAP enhanced the anti-tumor function mediated by NC in prostate cancer ***,the upregulation of YAP abrogated the anti-cancer activity of NC treatment in prostate cancer *** findings indicate that NC could be useful as a YAP inhibitor for the treatment of prostate cancer cells.

关键词： YAP Prostate cancer Nitidine chloride Hippo Growth Apoptosis Invasion

来源：评论

学校读者我要写书评

暂无评论

Protein Arginine Phosphatase YwlE Assay Based on colorimetric substrate p-nitrophenyl phosphate(pNPP)

Protein Arginine Phosphatase YwlE Assay Based on colorimetri...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Biling Huang Yan Liu Yufen Zhao Department of Chemical Biology College of Chemistry and Chemical EngineeringXiamen University Institute of Drug Discovery Technology Ningbo University

Protein arginine phosphorylation represents a novel post-translation modification that alters protein *** arginine phosphatase YwlE,a member of the low-molecular weight protein tyrosine phosphatase family,efficiently catalyzes the hydrolysis of phosphoarginine protein to fine-tune the cellular processes in Bacillus ***,we confirm that Zn2+ and Cu2+ inhibit YwlE with IC50 values of 0.11 ± 0.003 μM and 0.10 ± 0.008 μM using colorimetric substrate p-nitrophenyl phosphate（pNPP） which at an alkaline pH has an intense yellow color that can be measured at 405 ***（IAM） alkylates and H2 O2 oxidizes the Cysteine residues in active catalytic C（X）4 CR（S/T） motif to decrease the phosphatase ***,we evaluate commercial phosphatase inhibitors,metal cations and ions strengths that perform distinct ability of inhibition *** expect that the results contribute to biochemical experiments and identification phosphoarginine proteins in cell extract by Mass ***,we discovered that Ca2+ surprisingly improve the activity of YwlE with EC = 0.63 ± 0.03 μM.

关键词： Protein arginine phosphorylation YwlE p-nitrophenyl phosphate(pNPP) inhibitors

来源：评论

学校读者我要写书评

暂无评论

Oxidized ATM-mediated EMR plays a pivotal role in BCSC enrichment and stemness maintenance

Oxidized ATM-mediated EMR plays a pivotal role in BCSC enric...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者：柳满然重庆医科大学

经典的毛细血管扩张-共济失调突变基因(Ataxia-telangiectasia mutated gene, ATM)参与细胞周期调控、DNA损伤修复。近年来,我们与其他实验室揭示细胞中存在DNA损伤非依赖的ATM,即氧化型ATM (Oxidized ATM)。前期,我们运用芯片技术和磷... 详细信息

经典的毛细血管扩张-共济失调突变基因(Ataxia-telangiectasia mutated gene, ATM)参与细胞周期调控、DNA损伤修复。近年来,我们与其他实验室揭示细胞中存在DNA损伤非依赖的ATM,即氧化型ATM (Oxidized ATM)。前期,我们运用芯片技术和磷酸化组学分析,在乳腺肿瘤细胞中鉴定到数十个氧化型ATM靶基因和靶蛋白。其中,GLUT1、HK2、PKM2、DCA等参与肿瘤细胞能量代谢。氧化型ATM的活化,上调与葡萄糖吸收和糖酵解相关的GLUT1、HK2、PKM2表达、并上调与丙酮

关键词：氧化型糖酵解 ATM EMR Oxidized ATM-mediated EMR plays a pivotal role in BCSC enrichment and stemness maintenance

来源：评论

学校读者我要写书评

暂无评论

Regrowth-delay Body as a Bacterial Subcellular Structure Marking Multidrug Tolerant Persisters

Regrowth-delay Body as a Bacterial Subcellular Structure Mar...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Jiayu Yu Yang Liu Huijia Yin Zengyi Chang School of Life Sciences Peking University

Bacteria have been known to be able to enter a non-growing state when encountering unfit conditions,termed *** take a variable lag time and exhibit multidrug tolerance before resuming growth when placed in growth-supportive conditions,thus posing a great challenge in treating infectious *** to their non-inheritability,low abundance of existence,lack of metabolic activities,and high heterogeneity,properties of persisters remain poorly ***,we report our accidental discovery of a subcellular structure that we termed the regrowth-delay body,which is formed in nongrowing bacterial cells and sequesters selected proteins essential for cell *** formation occurs progressively in each cell and heterogeneously among individual *** only disassemble in re-growing cells to release proteins for immediate re-functioning when conditions become *** regrowth-delay bodies apparently function as a biological timer for bacterial growth resumption and could mark the multidrug tolerant *** observations implicate that persisters exhibit different depth of persistence,as determined by the status of their regrowth-delay *** findings also implicate that suppressing the formation and/or promoting the disassembly of regrowth-delay bodies are viable strategies for eradicating persisters.

关键词： Regrowth-delay body Lag time FtsZ Antibiotic tolerance

来源：评论

学校读者我要写书评

暂无评论

Long noncoding RNA GACAT3 regulates fatty acid metabolism and promotes proliferation by acting as a competing endogenous RNA in gastric cancer

Long noncoding RNA GACAT3 regulates fatty acid metabolism an...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Zhen Jiang Daogang Chen Hui Chen Xiaohan Mei Linyu Wang Wenqian Chen Zhuowei Luo Yaoyao Lu Department of Biochemistry School of Preclinical MedicineNorth Sichuan Medical College

It is widely demonstrated that aberrantly expressed lncRNAs are associated with various cancers,but it is still uncertain how they may become coordinated during the gastric ***,we report that a novel lncRNA GACAT3 regulates the expression of fatty acid synthesis enzyme(FASN) through increasing the expression of miR-497 in gastric *** found that the GACAT3 expression was positively correlated with the TNM staging and lymph node ***,the expression of GACAT3 and FASN were both negatively regulated by ***3 elevates the levels of FASN mRNA by acting as a competing endogenous *** overexpression of GACAT3 promotes cancer cell growth by promoting the G0/G1 to S phase *** summary,the current study highlights some evidence regarding the potential role of GACAT3 in gastric cancer and suggests that GACAT3 can be identified as the candidate target to suppress the carcinogenesis of gastric cancer.

关键词： GACAT3 competing endogenous RNA gastric cancer

来源：评论

学校读者我要写书评

暂无评论

Hydrophobin HFBI promoting active expression of pediocin PA-1 in Pichia pastoris and extending its application in emulsion

Hydrophobin HFBI promoting active expression of pediocin PA-...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Bo Wang Xiangxiang Wang Lu Ge Xiaotian Yang Haijin Xu Mingqiang Qiao The Key Laboratory of Molecular Microbiology and Technology Ministry of EducationCollege of Life SciencesNankai University

Pediocin PA-1,originating from Pediococcus acidilactici,is regarded as a broad-spectrum bacteriocin in lactic acid *** was reported that PA-1 could highly expressed in Pichia pastoris but without bioactivity,resulting from covalent binding by "collagen-like" materials presented in the *** this study,*** was used as the host for expression of fusion protein PA-1-linker-HFBI(PB),and Ultra-filtration and aqueous two-phase system(ATPs) extraction were employed to purify the recombinant protein *** activity assays showed the fusion protein PB retained the biological properties of native PA-1,indicated HFBI can overcome the unfunctional expression of PA-1 in *** addition,Water contact angle,oil emulsion antibacterial activity assay indicated PB also retained the biological properties of *** method of active expression of PA-1 in *** and the applications of PA-1 in food emulsion and antiseptic explored a novel technology to be used for other bacteriocins with the same feature of pediocin PA-1 molecule.

关键词： PA-1 Hydrophobin Fusion protein Antibacterial activity Emulsion

来源：评论

学校读者我要写书评

暂无评论

The adiponectin receptor PAQR-2 signaling couples low temperature with autophagy to promote longevity in C. elegans

The adiponectin receptor PAQR-2 signaling couples low temper...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Yuan-Li Chen Jun Tao Wei Tang Ke-Qin Zhang Cheng-Gang Zou State Laboratory for Conservation and Utilization of Bio-Resources in Yunnan Yunnan University

Temperature is a key determinant factor for lifespan in both poikilotherms and *** is believed that animals live longer at lower body ***,the precise role of temperature reduction in lifespan extension remains largely *** lysosomal degradation pathway,autophagy,also plays a fundamental role in lifespan extension promoted by a variety of genetic approaches in model ***,we report that autophagy serves as a boost mechanism for longevity at low temperature in the nematode Caenorhabditis *** adiponectin receptor AdipoR2 homolog PAQR-2 signaling detects temperature drop and consequently augments the biosynthesis of arachidonic acid,a ω-6 polyunsaturated fatty acid,in the *** acid in turn initiates autophagy in the *** autophagic activity in this tissue extends the lifespan,at least in part,by improving an age-dependent decline in total *** findings reveal that the adiponectin receptor PAQR-2 signaling acts as a regulator linking low temperature to autophagy to extend lifespan,and suggest that such a mechanism may be evolutionally conserved in diverse organisms.

关键词： Autophagy PAQR-2 receptor Temperature Longevity

来源：评论

学校读者我要写书评

暂无评论

第二届生物化学与分子生物学名词编写委员会第二次全体委员扩大会议在重庆召开

引用

中国科技术语 2018年第6期20卷 46-46页

作者：高素婷不详

2018年10月25-29日,"第二届生物化学与分子生物学名词编写委员会第二次全体委员扩大会议"与"中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议"在重庆召开。会议由第二届生物化学与分子生... 详细信息

2018年10月25-29日,"第二届生物化学与分子生物学名词编写委员会第二次全体委员扩大会议"与"中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议"在重庆召开。会议由第二届生物化学与分子生物学名词编写委员会主任李刚教授主持,会议特邀中国生物化学与分子生物学会理事长李林院士出席会议并致辞,全国科技名词委审定一室主任高素婷编审、第二届生物化学与分子生物学名词编写委员会全体委员参会,中国生物化学与分子生物学会专职副秘书长孙晓丽、山西大学生命科学院裴雁曦教授、重庆医科大学基础医学院雷云龙副教授、中山大学生命科学院屈良鹄教授、《中国生物化学与分子生物学报》编辑部贾竹青、陈苹等出席会议。

关键词：中国生物化学与分子生物学会科技名词编写重庆会员代表大会学术会议山西大学医科大学

来源：评论

学校读者我要写书评

暂无评论

脑硫脂对整合素αV亚基的表达调控

脑硫脂对整合素αV亚基的表达调控

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者：蔡倩倩董懿为吴兴中复旦大学

脑硫脂(Sulfatide,Sulfated Cerebroside)在肝细胞癌(hepatocellular carcinoma,HCC)中过表达,但脑硫脂表达升高与肝癌发生的联系还不十分清楚。我们的前期研究发现脑硫脂对整合素αV亚基表达的促进作用与αV亚基基因启动子上组蛋白H3K9... 详细信息

脑硫脂(Sulfatide,Sulfated Cerebroside)在肝细胞癌(hepatocellular carcinoma,HCC)中过表达,但脑硫脂表达升高与肝癌发生的联系还不十分清楚。我们的前期研究发现脑硫脂对整合素αV亚基表达的促进作用与αV亚基基因启动子上组蛋白H3K9/14和转录因子Sp1的乙酰化水平升高相关。为明确脑硫脂在肝癌细胞中对整合素αV亚基表达及其启动子上组蛋白乙酰化水平调控的分子机制,我们质谱分析了脑硫脂包被的环氧磁珠与肝癌细胞SMMC-7721裂解产物孵育得到的糖脂-蛋白复合物,得到了溴区包含蛋白1(BRD1,Bromodomain-containing protein 1)和配对两性螺旋蛋白(SIN3B,Paired amphipathic helix protein)2个蛋白评分最高且与乙酰化功能相关。我们利用脂质免疫印迹等方法验证了BRD1、SIN3B与脑硫脂间特异性相互作用;还通过构建BRD1基因的截短质粒,双荧光素酶报告基因实验发现BRD1是通过dN功能区与脑硫脂结合,并通过其PHD功能区直接与SIN3B及Sp1相结合。通过免疫荧光共定位及染色质蛋白免疫共沉淀(ChIP)等实验进一步发现通过脑硫脂处理后,BRD1与SIN3B发生由细胞质向细胞核内转位,其中BRD1与MYST乙酰基转移酶家族MOZ和HBO1结合,促进H3K9/14和Sp1乙酰化;同时脑硫脂可使SIN3B失去了与MAD1和HDAC2的结合亲和力,降低了整合素αV基因启动子上HDAC2的募集,阻止了组蛋白H3的去乙酰化,这两方面的共同作用使得整合素αV亚基表达增加。总之,在肝癌细胞中,脑硫脂通过与BRD1/SIN3B结合形成复合物入核促进调整合素αV亚基启动子上组蛋白H3K9/14的乙酰化,同时抑制了其去乙酰化,从而上调整合素αV亚基表达。

关键词：脑硫脂整合素溴区结合蛋白配对两性螺旋蛋白肝癌

来源：评论

学校读者我要写书评

暂无评论

Akt抑制剂MK-2206与顺铂协同抑制人三阴性乳腺癌MDA-MB-468细胞增殖

Akt抑制剂MK-2206与顺铂协同抑制人三阴性乳腺癌MDA-MB-468细胞增...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者：刘钰妮王世恩杨建林王艳林曹春雨三峡大学医学院肿瘤微环境与免疫治疗湖北省重点实验室

[目的]研究Akt抑制剂MK-2206对顺铂抗人三阴性乳腺癌MDA-MB-468细胞增殖的影响及MK-2206与顺铂联合应用在抗人三阴性乳腺癌临床治疗中的潜在价值。[方法]CCK-8法分析MK-2206对MDA-MB-468细胞的增殖抑制作用。Western blot检测MK-2206单... 详细信息

[目的]研究Akt抑制剂MK-2206对顺铂抗人三阴性乳腺癌MDA-MB-468细胞增殖的影响及MK-2206与顺铂联合应用在抗人三阴性乳腺癌临床治疗中的潜在价值。[方法]CCK-8法分析MK-2206对MDA-MB-468细胞的增殖抑制作用。Western blot检测MK-2206单独或联合顺铂对MDA-MB-468细胞内Akt磷酸化水平、凋亡相关基因Bax、Bcl-2及MDA-MB-468细胞浆内细胞色素C蛋白表达水平的影响。Annexin V/PI双染法检测细胞凋亡。使用Chou-Talalay中位效应与联合指数模型分析药物联合效应。[结果] MK-2206对MDA-MB-468细胞增殖具有剂量和时间依赖性抑制作用,其24小时的半数抑制浓度为11.23mmol/L。过表达组成性活化的Akt能够将顺铂对MDA-MB-468细胞的24小时半数抑制浓度从1.12mmol/L提高到1.75mmol/L,显著拮抗顺铂对MDA-MB-468细胞的增殖抑制效应。2mmol/L MK-2206可显著抑制MDA-MB-468细胞内Akt激酶活性,表现为Akt Thr308和Ser473磷酸化水平显著下降;顺铂处理不影响MDA-MB-468细胞内Akt磷酸化水平。单独使用2mmol/L MK-2206处理MDA-MB-468细胞24小时不能诱导MDAMB-468细胞凋亡,但可促进顺铂诱导的MDA-MB-468细胞内细胞色素C从线粒体向细胞浆的转移和Bax基因表达上调、Bcl-2基因表达下调,显著促进细胞凋亡发生。MK-2206和顺铂联合处理显示出抑制MDA-MB-468细胞增殖的药物协同效应。[结论] MK-2206顺铂产生药物协同效应抑制MDA-MB-468细胞增殖,并促进顺铂诱导的MDAMB-468细胞凋亡。提示顺铂与Akt抑制剂联合应用具有抗人三阴性乳腺癌治疗的临床价值。

关键词： Akt抑制剂 MK-2206 顺铂三阴性乳腺癌 MDA-MB-468细胞

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：