Aim of the study:To find a new strategy that can identify target genes of microRNAs effectively,conveniently and economically,we investigated the potential utilization of immunomagnetic beads in this area. Methods:A m...
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Aim of the study:To find a new strategy that can identify target genes of microRNAs effectively,conveniently and economically,we investigated the potential utilization of immunomagnetic beads in this area. Methods:A microRNA is tagged with a biotin group and transfected into suitable cells to fulfill its function specifically with target *** cells so the miRNA-mRNA complex can be captured on streptavidin *** the abstracted mRNA after amplified with *** BLAST
Human carbonyl reductase 1(CBR1) converts the antitumor drug and anthracycline daunorubicin(DNR) into the alcohol metabolite daunorubicinol(DNROL) with significantly reduced antitumor activity and cardiotoxicity,and t...
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Human carbonyl reductase 1(CBR1) converts the antitumor drug and anthracycline daunorubicin(DNR) into the alcohol metabolite daunorubicinol(DNROL) with significantly reduced antitumor activity and cardiotoxicity,and this limits the clinical use of *** of CBRl can thus increase the efficacy and decrease the toxicity of *** we report that(-)-epigallocatechin gallate(EGCG) from green tea is a promising inhibitor of *** directly
Although the fact that tumor cells have distinct metabolic patterns than those of normal cells was discovered by Otto Warburg in about 80 years ago,progress in searching molecular mechanism underline such phenomena wa...
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Although the fact that tumor cells have distinct metabolic patterns than those of normal cells was discovered by Otto Warburg in about 80 years ago,progress in searching molecular mechanism underline such phenomena was *** enzymes such as succinate dehydrogenase and fumarate hydratase mutations were frequently found in certain types of cancers provides the possibility that altered intermediary metabolites could be the direct cause of *** recent studies,together with studies from other labs,have demonstrated that mutations of NADP+
Atherosclerosis and its complications,in particular acute coronary heart disease is the leading cause of morbidity and mortality *** plays a central role in all stages of atherosclerosis from the initiation of atherom...
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Atherosclerosis and its complications,in particular acute coronary heart disease is the leading cause of morbidity and mortality *** plays a central role in all stages of atherosclerosis from the initiation of atheroma to plaque rupture.C-reactive protein(CRP),one of the prototypic human acute phase reactant,has important functions in host defense and *** evidence indicates that CRP is not merely a predictor
[Objective]To determine cerebral cortex fatty acid composition and content of rats.[Methods]Lipids were extracted from the cerebral cortex with methanol/ dichloromethane and were methylesterified using acetyl *** acid...
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[Objective]To determine cerebral cortex fatty acid composition and content of rats.[Methods]Lipids were extracted from the cerebral cortex with methanol/ dichloromethane and were methylesterified using acetyl *** acid methyl esters were then assayed by gas chromatography.[Results]Qualitative analysis results in this experimental conditions showed that there were 16 kinds of fatty acids in rat cerebral cortex,with major fatty acids
In order to clone the active catalase from bacteria,*** W3110 was cultivated and Genomic DNA was extracted and then was partially digested with restrictive enzyme Sau3AI. Genomic DNA fragments over 1.5kb were separate...
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In order to clone the active catalase from bacteria,*** W3110 was cultivated and Genomic DNA was extracted and then was partially digested with restrictive enzyme Sau3AI. Genomic DNA fragments over 1.5kb were separated and *** pUC18 after digested with BamHI was linked to the different DNA fragements partially digested with Sau3AI by ligases *** then the new ligated products were transformed into the *** competent DH5a. The transforments were incubated ananaer obically on
It has been known that food as one environmental factor can affect the expression of genes in animal cells,although we still lack examples of this kind of effect in which the detailed molecular mechanism is well ***,w...
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It has been known that food as one environmental factor can affect the expression of genes in animal cells,although we still lack examples of this kind of effect in which the detailed molecular mechanism is well ***,we found that the expression of a *** gene,che-2,is down regulated by oxidative stressed *** demonstrated that this down regulation is mediated by an *** noncoding RNA,OxyS *** stress induces the expression of OxyS in ***,which through a mechanism of feeding RNA interference,suppresses the expression of che-2 in ***
Human SIRTl(Silent Information Regulator Type 1) is an NAD+-dependent deacetylase protein which is an intermediary of cellular metabolism in gene silencing and *** has been extensively investigated and shown to delay ...
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Human SIRTl(Silent Information Regulator Type 1) is an NAD+-dependent deacetylase protein which is an intermediary of cellular metabolism in gene silencing and *** has been extensively investigated and shown to delay senescence,however less is known about the regulation of SIRTl during *** this study,we show that the peroxisome proliferator-activated receptor-γ(PPARγ), which is a ligand-regulated modular nuclear receptor that governs adipocyte differentiation and inhibits cellular
Alterations in cell growth and cell cycle regulations are commonly associated with the development of several human proliferative diseases,in particular cancer,and are believed to link with changes in cellular *** mos...
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Alterations in cell growth and cell cycle regulations are commonly associated with the development of several human proliferative diseases,in particular cancer,and are believed to link with changes in cellular *** most metabolic pathways have been well established several decades ago,how extracellular metabolic fuels signal to different metabolic pathways remain poorly understood. Recent proteomic analyses on protein acetylation identified potentially a large number of acetylated proteins in the
The ciliated protozoan Tetrahymena thermophila contains two distinct nuclei,the mitotic micronucleus and the amitotic *** a bipolar spindle does not form during macronuclear amitosis,microtubules are reassembled insid...
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The ciliated protozoan Tetrahymena thermophila contains two distinct nuclei,the mitotic micronucleus and the amitotic *** a bipolar spindle does not form during macronuclear amitosis,microtubules are reassembled inside the macronucleus and linking the macronucleus and cell cortex in the ***,a small GTPase belonging to the Ras superfamily,has been proposed to play a vital role in mitotic spindle *** further understand the function of Ran,we construct RAN gene overexpression *** overexpression strains RanOE-Cu428 and
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