RNA binding proteins(RBPs) are conventionally recognized as proteins that associate with RNA to accomplish their ***, accumulating evidences suggest the pervasive RBPchromatin interactions owing to discovery of regula...
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RNA binding proteins(RBPs) are conventionally recognized as proteins that associate with RNA to accomplish their ***, accumulating evidences suggest the pervasive RBPchromatin interactions owing to discovery of regulatory RNA's involvement on transcriptional ***, it is still unclear how extensively RBPs act on the chromatin *** we perform stringent nuclear fractionation coupled to mass spectrometry, providing genome-wide identification of human chromatin-enriched RBPs(Che-RBPs).Through a comprehensive analysis by integrating CLIP-seq, ChIP-seq and RNA-seq datasets for the same Che-RBPs, we demonstrate their distinct regulatory tendencies, including chromatin-biased, RNA-biased, and chromatin/RNAbalanced *** these Che-RBPs, we find QKI5 is specially tethered to the chromatin in monocytes and could promote monocytic ***5 ChIP-seq analysis presents a striking gene promoter binding, and its association to target loci is independent of the existence of RNA or certain transcription *** addition, promoter-located QKI5 activates the transcription of several monocyte-associated genes, including ***, the differentiation-promoting activity of QKI5 is largely dependent on CXCL2 rather than its RNA-binding *** together, these results provide an updated framework for the organization of RBPs in nucleus and indicate that Che-RBPs are versatile factors orchestrating gene expression at either transcriptional or post-transcriptional levels to adapt to complex regulatory networks in different cellular contexts.
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