目的:从骨骼肌形态变化及肌纤维类型转化的角度,探讨4周红景天苷灌胃对小鼠骨骼肌的影响。通过MHC、Tn I的检测,探讨红景天苷的作用效果,为进一步深入研究及应用提供依据。方法:筛选20只C57BL/6小鼠,综合体重和运动耐力情况分为对照组(PS)和红景天苷组(SAL),每组10只。SAL(HPLC98%),按50mg/kg的标准灌胃,每天每只小鼠灌0.1ml液体。分别测得两组小鼠0天、14天、28天的体重情况。28天灌胃结束后颈部脱臼处死取腓肠肌,测得PS和SAL小鼠肌湿重。冰冻切片免疫荧光技术检测MHC-I、MHC-Ⅱa、MHC-Ⅱb,Western blot检测Tn I表达,RTq-PCR检测MHC基因表达。结果:(1)4周SAL灌胃小鼠与对照组相比体重及肌湿重变化不大。(2)检测腓肠肌发现,SAL组肌横截面积显著增加,肌纤维数量可能增加。I型横截面积及基因表达显著高于PS,Ⅱb型显著低于PS。(3)SAL组的Tn I SS表达显著高于PS,而Tn I FS表达显著低于PS。(4)SAL组MHC-I基因表达升高,而MHC-Ⅱb表达下降。结论:4周SAL灌胃对小鼠体重、腓肠肌湿重影响不大,可提升MHC-I和Tn I SS,抑制MHC-Ⅱb和Tn I FS,促进Ⅱb→Ⅱd/x→Ⅱa→I转化。
Under normal conditions,estrogen stimulates the proliferation of endometrial epithelium,whereas progesterone inhibits estrogen-induced ***,the currently studies cannot completely elucidate the mechanisms of the antago...
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Under normal conditions,estrogen stimulates the proliferation of endometrial epithelium,whereas progesterone inhibits estrogen-induced ***,the currently studies cannot completely elucidate the mechanisms of the antagonistic actions of progesterone to *** RNAs(mi RNAs)are a class of small noncoding regulatory RNAs that work in the post-transcriptional regulation of gene *** researches indicate that estrogen and progesterone also can regulate mi RNAs in endometrial ***,whether P4 directly induces mi RNA expression in the endometrial epithelium,and whether these mi RNAs facilitate the antagonistic function of P4 to E2 remain *** this study,RNA was extracted from endometrial epithelial cells of ovariectomized mice,which were treated with E2 alone or a combination of E2 and *** RNA high-throughput sequencing with bioinformatics analysis was used to identify P4-induced mi RNAs,predict their potential target genes,and analyze their possible biological *** we focus on the P4-induced mi RNAs targeting cell cycle positive regulatory factors,study their expression regulative mechanisms of progesterone receptor,and their fuctions on proliferative regulation of endometrial epithelial cells in vivo and in *** found 146mature mi RNAs in the P4 group were significantly up-regulated by high-throughput ***-predicted with KEGG and GO pathway analysis showed many of them may participate in regulation of cell *** expression of 9 progesterone-induced micro RNAs were validated by quantitative real-time *** cluster mi RNAs,mmu-mir-145a-5p and mmu-mir-143-3p,which potential target genes are cyclin D2,cyclin E1 and cyclin E2,were selected for further *** expression of mmu-mir-145a-5p and mmu-mir-143-3p were examined in cultured mouse endometrial epithelial *** results showed that mmu-mir-145a-5p and mmu-mir-143-3p were expressed in a dose-dependent manner to P4,and the ex
血管钙化(VC)是许多疾病血管病变的共同病理表现,但仍缺乏治疗VC的有效手段。星状神经节阻滞(SGB)是临床常采用的交感神经阻滞术,鉴于交感神经在血管调节中的关键作用,我们认为SGB通过抑制内质网应激(ERS)改善VC。维生素D3肌注和尼古丁灌胃制备大鼠(180～200 g SD雄性)VC模型(VDN模型)。以侧入路法注射0.5%利多卡因0.3 ml(1次/d,共4w)行左侧SGB,同侧眼睑下垂为阻滞成功。与对照组相比,VDN血管组织钙含量和ALP活性及血浆ALP活性升高;Western blot结果显示SMC收缩表型标志calponin和SM22α表达降低,成骨细胞样表型标志BMP2和RUNX2表达升高,ERS标志GRP78、caspase12和CHOP表达升高。SGB治疗后,VC程度和SMC表型转化改善,激活的ERS被抑制。而且,ERS激动剂Tm能阻断SGB改善VC和SMC表型转化的作用;其抑制剂PBA具有与SGB相似的作用。SGB改善VC,逆转SMC表型转化,有应用于临床治疗VC的潜力。其作用与抑制ERS有关,ERS可能成为治疗VC和SMC表型转化的新靶点。
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