INTRODUCTION 17β-hydroxysteroid dehydrogenase type 8 (HSD17B8), also known as HKE6 or FABGL, is an important regulator of steroid and lipid metabolism. HSD17B8 mRNA expression has been measured in the reproductive or...
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INTRODUCTION 17β-hydroxysteroid dehydrogenase type 8 (HSD17B8), also known as HKE6 or FABGL, is an important regulator of steroid and lipid metabolism. HSD17B8 mRNA expression has been measured in the reproductive organs and peripheral tissues in humans, mice, and pigs . 17HSDB8 catalyzes the oxidation of estrone, testosterone, and dihydrotestosterone. Human HSD17B8 protein efficiently convert estradiol (E2) into estrone (E1) .
Marbling is an important trait regarding the quality of beef. Analysis of beef cattle transcriptome and its expression profile data are essential to extend the genetic information resources and would support further s...
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Marbling is an important trait regarding the quality of beef. Analysis of beef cattle transcriptome and its expression profile data are essential to extend the genetic information resources and would support further studies on beef cattle. RNA sequencing was performed in beef cattle using the Illumina High-Seq2000 platform. Approximately 251.58 million clean reads were generated from a high marbling(H) group and low marbling(L) group. Approximately 80.12% of the 19,994 bovine genes(protein coding) were detected in all samples, and 749 genes exhibited differential expression between the H and L groups based on fold change(>1.5-fold, p<0.05). Multiple gene ontology terms and biological pathways were found significantly enriched among the differentially expressed genes. The transcriptome data will facilitate future functional studies on marbling formation in beef cattle and may be applied to improve breeding programs for cattle and closely related mammals.
Adipogenesis is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. The recent explosion in knowledge have demonstrated that long noncoding RNAs(lncRNAs) are involved i...
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Adipogenesis is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. The recent explosion in knowledge have demonstrated that long noncoding RNAs(lncRNAs) are involved in adipogenic gene regulatory network. However, existing annotations of lncRNAs involved in adipogenic differentiation are derived from rodent and cell line studies, researches using primary cultures of farm animals in livestock are apparently needed. To comprehensively identify lncRNAs with potential functions during bovine adipogenesis, in the present study we performed Ribo-Zero-Seq to survey the transcriptome landscape of in vitro cultured bovine preadipocytes and differentiated adipocytes, which led to the annotation of 2,882 lncRNAs. The 2882 lnc RNAs shared many of the features of their mammalian counterparts: relatively shorter in length, significantly lower expression levels and fewer in exon number than RefSeq protein coding transcripts. Comparison of the lncRNAs expression profiles identified 41 specifically regulated lncRNAs during adipogenic differentiation. Integrative computational analyses associated these lncRNAs with several signaling pathways involved in lipid metabolism, including steroid biosynthesis, PPAR signaling pathway, glycolysis/gluconeogenesis, and fatty acid metabolism. Notably, one of the regulated lncRNAs, NONBTAT021534, was greatly enriched in adipose tissue and exhibited promoter binding of peroxisome proliferator-activated receptor γ(PPARγ), indicating a functional role in adipogenesis. In summary, our data provide a valuable genomic resource for the identification of lncRNAs with potential functions in adipogenic differentiation.
Zinc finger, BED-type containing 6(ZBED6) is an important transcription factor in placental mammals, affecting development, cell proliferation and growth. In this study, we found that the expression of the ZBED6 and I...
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Zinc finger, BED-type containing 6(ZBED6) is an important transcription factor in placental mammals, affecting development, cell proliferation and growth. In this study, we found that the expression of the ZBED6 and IGF2 were upregulated during C2C12 *** IGF2 expression levels were negatively associated with the methylation status in beef cattle(P<0.05). A luciferase assay for the IGF2 intron 3 and P3 promoter showed that the 439 G-SNP-pGL3 in driving reporter gene transcription is significantly higher than that of the 439 A-SNP-pGL3 construct(P < 0.05). An over-expression assay revealed that ZBED6 regulate IGF2 expression and promote myoblast differentiation. Furthermore, knockdown of ZBED6 led to IGF2 expression change in vitro. Taken together, these results suggest that ZBED6 inhibits IGF2 activity and expression via a G to A transition disrupts the interaction. Thus we propose that ZBED6 plays a critical role in myogenic differentiation.
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