Introduction Canine parvovirus(CPV)is a single-stranded DNA virus and a member of the genus parvovirus of the family *** has been continued to mutate,Up to the present time,the virus has undergone mutations from CPV-2...
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Introduction Canine parvovirus(CPV)is a single-stranded DNA virus and a member of the genus parvovirus of the family *** has been continued to mutate,Up to the present time,the virus has undergone mutations from CPV-2to CPV-2a,CPV-2b,new-type CPV-2a,new-type CPV-2b,CPV-2c,CPV-2c(a)and CPV-2c(b).The CPV disease has been shown to currently have a multi-type or mixed infection in *** found considerable variation of CPV-2a based on
The aim of this study was to construct the recombinant strain expressing OmpC gene of Salmonella pullorum by Bacillus subtilis expression system and evaluate immune activity of recombinant *** Bacillus subtilis WB800 ...
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The aim of this study was to construct the recombinant strain expressing OmpC gene of Salmonella pullorum by Bacillus subtilis expression system and evaluate immune activity of recombinant *** Bacillus subtilis WB800 n that is lack of extracellular enzyme was selected as host *** result showed that the recombinant Bacillus subtilis pHT43/WB800n-OmpC expressing OmpC gene was successfully constructed,the molecular weight of the expressed recombinant protein was about 37 kDa by the experiments of SDS-PAGE and western blot confirmed the recombinant protein had good ***,the humoral immunity of chick can be induced by oral immunization with recombinant strain of Bacillus subtilis pHT43/WB800n-OmpC,The challenge of Salmonella showed that the immunity protection rates was 76.93%.Based on experiments,it is concluded that the gene OmpC of Salmonella firstly expressed in Bacillus subtilis,which lay a theoretical foundation for Salmonella immune mechanism and developing of genetic engineering probiotics that play a dual role after oral immunization.
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