Thymosinβ4(Tβ4) is an important molecule functioning in angiogenesis,wound healing,cancer metastasis and cardiac repair and so *** Tβ4 is synthesized chemically for research and this is *** order to obtain sufficie...
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Thymosinβ4(Tβ4) is an important molecule functioning in angiogenesis,wound healing,cancer metastasis and cardiac repair and so *** Tβ4 is synthesized chemically for research and this is *** order to obtain sufficient biologically active Tβ4 economically,we cloned and overexpressed this protein in an Escherichia ***.A high level expression of GST-Tβ4 fusion protein was obtained and purified *** biological activity and function of this recombinant Tβ4 was evaluated by cell proliferation assay using prepared spleen *** data indicated that the recombinant Tβ4 could induce lymphocyte proliferation and *** Tβ4 with bioactive is expressed efficiency and purified,becoming the foundation of the further study of Tβ4.
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