Cancer continues to increase with increasing age of the *** a number of situations,the malignancy of tumors is detected only at advanced stages when administration of chemotherapeutic drugs is toxic to healthy *** nan...
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Cancer continues to increase with increasing age of the *** a number of situations,the malignancy of tumors is detected only at advanced stages when administration of chemotherapeutic drugs is toxic to healthy *** nanotubes(CNTs) comprised of thin sheets of benzene rings rolled up into the shape of seamless cylinders has recently attracted significant attention for use in biomedical applications[1-6].A limited number of single-wall metal-organic nanotubes(SWMONTs) have been synthesized,while a majority of reports have focused solely on structural details,SWMONTs have already shown promise in highly selective adsorptions[7].Both single-walled nanotubes(SWNT) and multi-walled nanotubes(MWNT) are being considered as a drug delivery nanocarrier[8],because they were observed to cross cell membranes[9-11]and exhibit blood circulation half-lives of the order of hours[12].The possible mechanisms proposed for CNT-drug interaction are:(a) the absorption of active components of drug within the CNT mesh,(b)non-covalent or covalent linkage of drug molecules,peptides,and nucleic acids to the exterior walls of the CNTs,and(c) the use of CNT channels as ***,we used the reported self-assembled functionalized SWMONTs to realize drug delivery of 5-Fluorouracil(5-FU),which could be assigned to the first CNT-drug interaction[13].The characterization of the material indicated the presence of drug associated with SWMONTs(5FU@SWMONTs) in a proportion of 0.33 g 5-FU per 1.0 g of *** drug demonstrated a slow release profile where 79.6%of the drug was released in PBS buffer(pH7.4) in 24 hours,while 75%in PBS buffer(pH5.7) in 24 *** cytotoxic tests were carried out against two cell lines:4T1 and *** highest lethality of cancer cells occurred at the highest concentration of 5FU@SWMONTs for the longest period of *** results demonstrate that the cytotoxicity of 5FU@SWMONTs to cancer cells was improved by the SWMONTs component.
Background & Aim:As the leading cause of liver dysfunction all over the world,non-alcoholic fatty liver disease(NAFLD) is a clinical metabolic syndrome with fatty accumulation in hepatocytes,nevertheless,without a...
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Background & Aim:As the leading cause of liver dysfunction all over the world,non-alcoholic fatty liver disease(NAFLD) is a clinical metabolic syndrome with fatty accumulation in hepatocytes,nevertheless,without alcohol *** recent years,morbidity of NAFLD has grown in Eastern and Western countries,and the diet composition has become an important health *** and NASH are involved in the spectrum of NAFLD,NASH is easier develop into liver fibrosis,cirrhosis,and even hepatocellular *** clinical,hver biopsy as the gold standard to assess levels of steatosis,inflammation and fibrosis in NAFLD is invasive,costly and associated with possible *** kinds of noninvasive biomarkers for evaluating NAFLD have been investigated but none performed well ***,it is still interest to discover noninvasive method to help detecting *** published data showed that quality of fatty acids(FA) accumulated in liver is associated with the degree of liver damage in *** about serum FA variation in NAFLD is *** study aims to investigate the correlation between serum FA composition and the hepatic steatosis,inflammation,hepatocellular ballooning scores and even liver FA composition in the influence of high-fat diet(HFD) in ***:ICR mice(10mice/group) were fed with HFD or control diet(CD) for 4,6 or *** were collected for pathology and NAFLD activity score(NAS) *** and liver FA composition was analyzed by gas chromatography(GC).The correlation analysis was determined with Pearson correlation ***:Induced by HFD,mice got fatty liver disease of different level without obvious fibrosis,and the liver damage level increased as the HFD feeding time went *** could be diagnosed at the 4th week of HFD feeding and NASH could be diagnosed at the 6th and 8th *** fibrosis has not occurred from the beginning to the end of the *** composition analysis in mice serum and
The aim of the study was to develop a multiplex real-time PCR method to identify Campylobacter jejuni containing mutations commonly associated with macrolide resistance.A multiplex fluorescence real-time PCR assay was...
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The aim of the study was to develop a multiplex real-time PCR method to identify Campylobacter jejuni containing mutations commonly associated with macrolide resistance.A multiplex fluorescence real-time PCR assay was developed based on TaqMan minor groove binder(MGB) *** VS1-MGB probe was designed based on the VS1 gene and was used to identify Campylobacter *** 23 S rDNA-MGB probe was designed to distinguish macrolide resistance mutations in 23 S rDNA,while 57D-MGB and 74D-MGB were designed to detect resistance mutations in ribosomal protein *** specificity and accuracy of our method were identical to the conventional biochemical tests,mapA PCR,minimum inhibitory concentration(MIC) determination and DNA *** linear detection limit of the method was 003 ng genomic DNA and three colony formation unit(CFU) per *** 6 of 18 cases,the nature of Erythromycin resistance could be correctly determined from natural isolates;absence of the tested mutations was demonstrated in the remaining four resistant ***,a multiplex TaqMan MGB real-time PCR assay with high specificity and accuracy was developed to simultaneously identify *** and detect the gene mutations associated with macrolide *** multiplex method can potentially simplify the identification of Camp,jejuni and determine macrolide resistance due to mutations in 23 S rDNA or ribosomal protein *** method has a potential for application in different research areas and molecular surveillance.
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