90只大鼠随机分为5组(n=18),分别肌肉注射10μg、50μg、100μgpCIS、50μgpcDNA3.1和100μL生理盐水.20天后加强免疫一次,对每组中的12只大鼠进行两次加强免疫,观察卵泡发育和产仔.结果发现抑制素抗体P/N值随着免疫次数的增加而提高.100μg剂量组两次和三次免疫的成熟卵泡发育数分别比对照组多6.9和7.5个(P<0.05).三次免疫后大鼠成熟卵泡发育数比两次免疫后显著提高(35.2±2.73 vs 31.0±0.92,P<0.05).免疫组和阳性组的胎盘数和窝产仔数分别高于对照组(P>0.05)和阴性组(P<0.05).pCIS三次免疫后阳性鼠的抗体水平与成熟卵泡发育数和胎盘数的相关系数分别为0.45(P>0.05),0.77(P<0.05).pCIS免疫大鼠动情期和产后血浆FSH水平高于对照组,抗体阳性鼠的血浆FSH水平高于阴性鼠,其中两次免疫后阳性组动情期FSH浓度极显著高于阴性组(P<0.01).这些结果表明,抑制素基因免疫大鼠可促进大鼠的卵泡发育,提高血浆FSH水平,为抑制素基因免疫大动物提供了实验依据.
The chemical composition and microstructure of seven uroliths and four urinary sediment samples associated with the feeding of high-level cottonseed meal diet to buffalo calves were examined by chemical qualitative an...
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The chemical composition and microstructure of seven uroliths and four urinary sediment samples associated with the feeding of high-level cottonseed meal diet to buffalo calves were examined by chemical qualitative analyses, scanning electron microscopy ( ), X-ray diffraction, and X-ray energy dispersive spectrometry ( ). Struvite was a major component of kidney stones and of some bladder stones. The kidney stone sample appeared cracked under low power under, aggregated into tiny balls under high power, and as a bladelike structure under even higher power. The bladder stone samples appeared finely granular or granular with various forms of prismatic crystals. The urinary sediments were prismatic crystals, with granules. The newly found prismatic crystals, which were rich in potassium and similar to struvite in crystal structure, were identified as potassium magnesium phosphate (KMgP04·6H2O) in some bladder stones and urinary sediments. However, crystals which contained Mg and P only, which had been used for struvite identification, were not found by examination in urinary sediments from fresh urine samples of buffalo calves fed the high-level cottonseed meal diet.
Objective: The response to intravenous glucose loading in the buffalo using the intravenous glucose tolerance test (IGTT) was investigated to provide a reference for intravenous glucose injection in buffaloes. Method:...
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Objective: The response to intravenous glucose loading in the buffalo using the intravenous glucose tolerance test (IGTT) was investigated to provide a reference for intravenous glucose injection in buffaloes. Method: Twelve healthy, fasted, male swamp buffaloes were divided into three groups. Group I: six buffaloes were given 50% glucose at a dosage of 1 g/kg body weight via the jugular vein. Group II: three buffaloes received normal saline. Group III: three buffaloes were not injected. Blood samples were taken from the opposite vein at 60 and 10 min pre-injection (pre60 and pre10), and at 1, 5, 10, 30, 60, 120, 180, 240, 300, 360 and 420 min post-glucose injection (PGI). Plasma glucose was analyzed by the oxidase method. Insulin and glucagon were soon determined with a human radioimmunoassay kit. The insulin (pmol/l)/glucose (mmol/l) ratios (IGR) were also calculated for each sampling time. Results: Mean plasma glucose, insulin and glucagon concentrations of buffaloes in groups II and III were similar at all the sampling times (p>0.05) and the curves of the IGR for group II and group III were flat throughout. Group I Buffaloes showed an immediate 20 times increase in the mean plasma glucose concentration PGI, over the pre60 and pre10. The peak plasma insulin concentration occurred at 30 min PGI. The mean plasma glucose and insulin concentrations remained above pre-administration levels untill 420 min PGI (p<0.05). However, the mean plasma glucagon concentrations were different only at 1 and 5 min PGI sampling times. The curve of the IGR for group I showed an initial decrease at 1 min PGI, and fluctuated from 10.18 to 25.55 for the remainder of the sampling period. The correlation analysis showed that the mean plasma glucose concentration was positively correlated with insulin level (r=0.73, p<0.005), and significantly negatively correlated with mean plasma glucagon (r=-0.58, p<0.05). The mean plasma insulin level did not show significant correlation with the gluca
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