Fluoride and sulfur dioxide are two well known environ ment pollutants,co-existing in some places,which present a serious threat to public *** order to explore the potential toxic effects of the two pollutants on the ...
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Fluoride and sulfur dioxide are two well known environ ment pollutants,co-existing in some places,which present a serious threat to public *** order to explore the potential toxic effects of the two pollutants on the male reproduction and the mechanism of their interactio n,sexually matured rats and unmatur ed mice were treated with fluoride （100mg/L NaF） and sulfur dioxide （39.3 mg/m SO,4hr/day）,and fluoride（150mg/L NaF） and sulfur dioxide（26.2mg/m SO,3hr/day）, *** diverse methods as radioimmunoassay, transmission electronic microscopic, flow cytometry（FCM） and Terminal deoxynucleotidyl Transferase Bioti n-dUTP Nick End Labeling（TUNE L）,immunohidtochemidtry and Real-time PCR,this study dynamica lly observed the changes of sperm quality,morphology and subcellular structure of testis,protein level, antioxidation and the activity of relative enzymes in testis,apoptosis in seminaferous cell and the expressi on of p53,bcl-2,bax gene in these two kinds of ***, miRNA microarray,a new explored technology,was used to examined the changes of non-coded RNA in order to study the mechanism of effects of these two elements on the *** results indicate that Fluoride and sulfur dioxide can induce the damage of male reproduc tive system including the changes in structure of tissues,cell and molecu lar level so that significantly destroy the reproductive *** toxic effect of fluoride is in coordination with sulfur dioxide,the mechanism of which may be that fluoride and sulfur dioxide produce oxidative stress in testis tissue,disrupt the DNA single chain,activate DNA-PK, and induce phosphorylation and activation of p53 *** re,the activated p53 protein can enhance the expression of apoptosis gene such as bcl-2,bax et *** start spermatogenic cells apoptosis, finally impair spermiogenesis. Maybe the regulation of miRNAs existed during this process.
Ketosis is a common metabolic disorder frequently observed in dairy cows during the early lactation *** is a metabolic condition characterized by increased levels of ketone bodies in blood,urine and *** major ketone b...
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Ketosis is a common metabolic disorder frequently observed in dairy cows during the early lactation *** is a metabolic condition characterized by increased levels of ketone bodies in blood,urine and *** major ketone body is beta-hydroxybutyrate （BHBA）.Determination of BHBA in milk samples is an important tool in the diagnosis of subclinical/clinical ketosis in dairy *** this report, we describe a novel method based on the traditional spectrophotometric approach for measuring BHBA levels in milk of dairy *** two commercial dairy farms,milk samples were taken from dairy cows within 2 months after *** spectrophotometric approach involve d two ***,in the prese nce of NAD,was oxidized by BHBA dehydrogenase to AcAc and NADH. Then NADH and nitroblue tetrazoli um were reduced by diaphorase to fo rmazan and NAD,*** amount of formazan was measured *** were prepa red as follows:milk was deproteiniz ed with perchloric acid,centrifuged, the supernatant was neutralized with KOH *** standard curve was made by a series of dilutions the BHBA standard solution.A reaction buffer was prepared,and comprised Tris buffer,Triton x-100,NBT,BHB A dehydrogenase,diaphorase and oxamic ***-buffer（pH 8.5）, the reaction solution and prepared milk sample were added to centrifug e tubes,in order,and shaken vigorou *** resulting mixture was kept at 30 min at room temperature in the dark and assayed using a UV spectro *** following paramete rs were established:linearity,recove ry,repeatability,decision limit and *** fluorometric detectio n procedure as *** sample buffer comprised Tris-buffer pH 8.5, oxamic acid,NAD,rezasurin,Triton X-100,BHBA dehydrogenase and *** end-point process was read after 20 *** results showed that the standard-curve equa tion of the UV spectrophotometer method was y=0.2582x+0.0269 （R=0.9967）.The reaction buffer did not react with AcAc,L-s
实验旨在从雌激素α受体(ERα)和孕激素受体(PR)的角度探讨PMSG(Pregnant Mare SerumGonadotropin)和不同时间的Anti-PMSG处理的小鼠在卵巢、输卵管和子宫中分布是否存在显著差异。采用20只8周龄母鼠,根据处理方式的不同随机分为4个处理...
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实验旨在从雌激素α受体(ERα)和孕激素受体(PR)的角度探讨PMSG(Pregnant Mare SerumGonadotropin)和不同时间的Anti-PMSG处理的小鼠在卵巢、输卵管和子宫中分布是否存在显著差异。采用20只8周龄母鼠,根据处理方式的不同随机分为4个处理组:PMSG组、PMSG处理后36h注射Anti-PMSG-组、PMSG处理后42h注射Anti-PMSG-组、PMSG处理后48h注射Anti-PMSG-组。前三组在PMSG处理后48h将小鼠颈椎脱臼处死,第四组于PMSG处理后54h将小鼠颈椎脱臼处死;取小鼠卵巢、输卵管、子宫固定,采用免疫组织化学法分别观察组织中ERα和PR分布情况。发现此试验中不同时间的Anti-PMSG的作用影响了经PMSG处理后的小鼠卵巢、输卵管和子宫中的ERα和PR分布,其中Anti-PMSG-36h对子宫中ERα和PR的调节效果更为显著,而AP42h和AP48h也不同程度影响了各组织中ERα和PR的动态分布。
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