Three truncated α-mannanase from Capra hircus lysosomal (chLAMa, chLAMc and chLAMd) was expressed and secreted in Pichia *** recombinant engineered strain for chLAMc productions was stable, and the maximum yield of i...
Three truncated α-mannanase from Capra hircus lysosomal (chLAMa, chLAMc and chLAMd) was expressed and secreted in Pichia *** recombinant engineered strain for chLAMc productions was stable, and the maximum yield of it reached 1.9 U ***, No mannanase activity was detected in supematant of engineered strains GS 115/pPIC9K-chLAMa and GS 115/*** exhibited an optimal temperature at 55℃ for the 10-min assay at pH 6.0 which is similar pH optima, temperature optima to its full-length protein (chLAM).
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